FastQCFastQC Report
Thu 26 May 2016
SRR1043372_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1043372_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1004825
Sequences flagged as poor quality0
Sequence length52
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT784567.807926753414773No Hit
CCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTGCT88940.8851292513621776No Hit
CTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTGCTT85150.847411240763317Illumina Single End Adapter 1 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTGCT58170.5789067748115343No Hit
CGCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTGC43390.43181648545766677No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCG33370.33209762894036277No Hit
TCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTGCT31320.3116960664792377No Hit
CGTTCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCT22900.2279003806632996No Hit
ACCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTGC22520.22411862762172519No Hit
GCCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTGC20840.20739929838529095No Hit
AGCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTGC18880.18789341427611772No Hit
ACTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTGCT17340.17256736247605306No Hit
TCCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTGC16640.16560097529420545No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCT16550.16470529694225364No Hit
CGTCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTG15620.1554499539720847No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTT15470.15395715671883162No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTC15270.1519667603811609No Hit
TGCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTGC13170.13106759883561814No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCC12980.12917672231483093No Hit
TGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTGCTTG12900.12838056377976265Illumina Single End Adapter 1 (95% over 22bp)
GGCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTGC12630.12569352872390716No Hit
ATCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTGC11290.11235787326151321No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGT10950.10897419948747294No Hit
ATGCCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCT10250.10200781230562536No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCGG800.046.0000041
TCCGCGG750.046.0000041
GCGAATA206.3110725E-446.0000048
TTGTCGC351.01939804E-746.00000437
TCGGGTA405.6097633E-946.0000044
CCCAGTT206.3110725E-446.00000418
CCAGCGG700.046.0000041
TTACTGA206.3110725E-446.00000430
GGTCACT206.3110725E-446.0000048
ACGGGTC405.6097633E-946.0000044
CTAACGG700.046.0000041
GAACGTT206.3110725E-446.0000048
GTCCGCA206.3110725E-446.00000430
AGTTCCG206.3110725E-446.00000423
ATGCGGC405.6097633E-946.0000042
GCTACGA405.6097633E-946.00000410
CCAACGG351.01939804E-746.0000041
AGCTACG405.6097633E-946.0000049
CCACGGC206.3110725E-446.0000042
TGTGTCA405.6097633E-946.00000420
Produced by FastQC (version 0.11.5)