##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043372_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1004825
Sequences flagged as poor quality	0
Sequence length	52
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.1940795660936	33.0	31.0	34.0	30.0	34.0
2	32.5194188042694	34.0	31.0	34.0	31.0	34.0
3	32.56415296195855	34.0	31.0	34.0	31.0	34.0
4	36.10895330032593	37.0	35.0	37.0	35.0	37.0
5	36.00052645983131	37.0	35.0	37.0	35.0	37.0
6	36.26570198790834	37.0	37.0	37.0	35.0	37.0
7	36.18619958699276	37.0	37.0	37.0	35.0	37.0
8	36.16577613017192	37.0	37.0	37.0	35.0	37.0
9	37.70786554872739	39.0	38.0	39.0	35.0	39.0
10	37.56193566043839	39.0	37.0	39.0	35.0	39.0
11	37.249747966063744	39.0	37.0	39.0	34.0	39.0
12	37.22005622869654	39.0	37.0	39.0	34.0	39.0
13	37.22554076580499	39.0	37.0	39.0	34.0	39.0
14	38.62669221008633	40.0	38.0	41.0	34.0	41.0
15	38.74763963874307	40.0	38.0	41.0	35.0	41.0
16	38.76647774488095	40.0	38.0	41.0	35.0	41.0
17	38.73984126589207	40.0	38.0	41.0	35.0	41.0
18	38.44201527629189	40.0	38.0	41.0	35.0	41.0
19	38.14781479362078	40.0	37.0	41.0	34.0	41.0
20	37.82770631702038	40.0	35.0	41.0	34.0	41.0
21	37.747188813972585	40.0	35.0	41.0	34.0	41.0
22	37.69146667330132	39.0	35.0	41.0	34.0	41.0
23	37.686863881770456	39.0	35.0	41.0	34.0	41.0
24	37.59460702112308	39.0	35.0	41.0	34.0	41.0
25	37.472614634389075	39.0	35.0	41.0	33.0	41.0
26	37.44163112979872	39.0	35.0	41.0	33.0	41.0
27	37.48796059015251	39.0	35.0	41.0	34.0	41.0
28	37.44361655014555	39.0	35.0	41.0	33.0	41.0
29	37.440561291767224	39.0	35.0	41.0	33.0	41.0
30	37.38503620033339	39.0	35.0	41.0	33.0	41.0
31	37.24990620257259	40.0	35.0	41.0	33.0	41.0
32	37.08279650685443	40.0	35.0	41.0	33.0	41.0
33	36.92591545791556	40.0	35.0	41.0	33.0	41.0
34	36.7438927176374	40.0	35.0	41.0	32.0	41.0
35	36.623705620381656	40.0	35.0	41.0	31.0	41.0
36	36.556797452292685	40.0	35.0	41.0	31.0	41.0
37	36.52729181698306	39.0	35.0	41.0	31.0	41.0
38	36.4589077700097	39.0	35.0	41.0	31.0	41.0
39	36.43447366456846	39.0	35.0	41.0	31.0	41.0
40	36.38369865399448	39.0	35.0	41.0	31.0	41.0
41	36.3774547806832	39.0	35.0	41.0	31.0	41.0
42	36.28637125867689	39.0	35.0	41.0	31.0	41.0
43	36.150825268081505	39.0	35.0	41.0	31.0	41.0
44	35.99032269300624	39.0	35.0	41.0	30.0	41.0
45	35.89670838205658	38.0	35.0	41.0	30.0	41.0
46	35.85983031871221	38.0	35.0	41.0	30.0	41.0
47	35.754294528898065	38.0	35.0	40.0	29.0	41.0
48	35.70811136267509	38.0	35.0	40.0	29.0	41.0
49	35.642208344736645	38.0	35.0	40.0	29.0	41.0
50	35.48213569527032	38.0	35.0	40.0	28.0	41.0
51	35.389217027840665	38.0	35.0	40.0	28.0	41.0
52	35.089155823153284	37.0	35.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	1.0
14	5.0
15	8.0
16	39.0
17	72.0
18	159.0
19	342.0
20	634.0
21	1133.0
22	1782.0
23	2942.0
24	5429.0
25	9417.0
26	13272.0
27	14091.0
28	13067.0
29	13067.0
30	14868.0
31	18404.0
32	23018.0
33	30390.0
34	53631.0
35	101045.0
36	113147.0
37	76246.0
38	124135.0
39	373503.0
40	977.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.698330555071777	19.8446495658448	25.05043166720573	30.40658821187769
2	34.7916303834001	19.094668225810466	26.12444953101286	19.989251859776576
3	21.329982832831586	21.05928893090837	39.13696414798597	18.473764088274077
4	22.077874256711368	23.401985420346826	37.01719204836663	17.502948274575175
5	19.373323713084368	28.23834996143607	32.87477919040629	19.513547135073274
6	69.37407011171099	1.5358893339636255	24.36891996118727	4.721120593138109
7	69.958549996268	1.3328689075212101	20.154653795437017	8.553927300773767
8	56.91389047844152	2.8066578757495084	29.558082253128653	10.721369392680318
9	32.500982758191725	16.270395342472572	32.20292090662553	19.025700992710174
10	25.05491005896549	18.483517030328663	43.92536013733735	12.536212773368497
11	21.69581767969547	15.26365287487871	42.01378349463837	21.02674595078745
12	15.720150274923494	20.33289378747543	44.79207822257607	19.154877715025005
13	11.294404498295723	23.352424551538824	44.92642997536885	20.426740974796605
14	9.620282138680865	23.171796083895206	46.15649491204936	21.051426865374566
15	10.246162267061429	18.295325056601897	50.577165178016074	20.881347498320604
16	10.72316074938422	25.93884507252507	40.0997188565173	23.23827532157341
17	12.743612071753788	22.86298609210559	42.464409225487024	21.928992610653598
18	14.145945811459706	29.21374368671162	40.45808971711492	16.182220784713756
19	14.277212449929092	24.31766725549225	36.789888786604635	24.615231507974027
20	11.899982584032045	30.910656084392805	35.50289851466673	21.686462816908417
21	12.907222650710324	24.65533799417809	37.38501729156818	25.052422063543407
22	10.985793546139876	27.28146692210086	32.66081158410669	29.071927947652576
23	10.6966884780932	24.04577911576643	43.88017814047222	21.377354265668153
24	16.680815067300276	21.314159181947108	39.61505734829448	22.38996840245814
25	12.626377727464982	25.672629562361603	38.35294703057746	23.34804567959595
26	16.001393277436367	21.392282238200682	37.04515711691091	25.561167367452043
27	21.45189460851392	21.911278083248327	37.70477446321499	18.932052845022763
28	14.633095315104619	21.302017764287314	36.02766650909363	28.03722041151444
29	21.32301644564974	21.399447665016297	34.58831139750703	22.689224491826934
30	19.972632050357028	22.12912696240639	30.777697609036398	27.120543378200185
31	20.69723583708606	21.78866966884781	34.37782698479835	23.136267509267782
32	18.33990993456572	21.31495534048217	34.71221356952704	25.632921155425077
33	14.38827656557112	24.756450128131764	35.05137710546612	25.80389620083099
34	13.121389296643693	24.58179284950116	39.827532157340826	22.46928569651432
35	15.326449879332221	23.13338143457816	37.724877466225465	23.815291219864154
36	13.150150523723036	33.88022790038066	34.15530067424676	18.81432090164954
37	12.54387579926853	29.264896872589752	37.511009379742745	20.680217948398973
38	11.684721220112955	29.63262259597442	32.38305177518473	26.299604408727888
39	19.108800039807928	26.94011395019033	33.892966436941755	20.058119573059983
40	13.617396063991242	22.860597616500385	38.9112532032941	24.610753116214266
41	18.207548578110615	24.253676013236138	31.14572189187172	26.393053516781528
42	18.18545517876247	18.193416764113156	39.279476525763194	24.341651531361183
43	21.526235911725923	22.051003906152815	36.770780981763	19.651979200358273
44	14.889259323762843	24.953399845744283	36.93737715522604	23.21996367526684
45	17.93158012589257	21.80250292339462	36.05244694349762	24.213470007215186
46	22.25551712984848	20.04030552583783	34.25312865424328	23.45104869007041
47	15.149603164730177	18.178787351031275	39.69009529022466	26.981514194013883
48	16.809991789615104	16.78332047869032	34.40877764784913	31.997910083845444
49	18.999925360137336	16.6105540765805	40.3258278804767	24.063692682805463
50	16.872092155350433	15.825541761003159	41.086258801283805	26.2161072823626
51	14.421615704227102	15.49633020675242	39.02968178538552	31.05237230363496
52	13.53320229890777	15.085512402657178	48.37618490781977	23.005100390615283
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	514.0
1	1790.5
2	3067.0
3	43574.0
4	84081.0
5	45147.5
6	6214.0
7	6025.0
8	5836.0
9	6301.5
10	6767.0
11	7218.5
12	7670.0
13	7470.0
14	6730.5
15	6191.0
16	5625.0
17	5059.0
18	4567.5
19	4076.0
20	3787.5
21	3499.0
22	3272.0
23	3045.0
24	3042.0
25	3039.0
26	3640.5
27	4242.0
28	4232.5
29	4223.0
30	4689.5
31	5156.0
32	5940.0
33	6724.0
34	8183.0
35	9642.0
36	11218.5
37	12795.0
38	14380.0
39	20363.0
40	24761.0
41	37694.0
42	50627.0
43	73809.0
44	96991.0
45	129051.5
46	161112.0
47	163392.0
48	165672.0
49	142233.0
50	118794.0
51	93235.0
52	67676.0
53	52340.5
54	37005.0
55	30367.0
56	23729.0
57	20289.5
58	16850.0
59	14263.5
60	11677.0
61	9794.0
62	7911.0
63	6552.5
64	4169.0
65	3144.0
66	2703.0
67	2262.0
68	2058.0
69	1854.0
70	1561.0
71	1268.0
72	1341.5
73	1415.0
74	1102.5
75	790.0
76	618.5
77	447.0
78	411.0
79	375.0
80	228.5
81	82.0
82	61.5
83	41.0
84	46.5
85	52.0
86	31.0
87	10.0
88	5.0
89	4.5
90	9.0
91	4.5
92	0.0
93	0.0
94	0.0
95	1.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1004825.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.42906102293031
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.7372892162593	20.67852833771566
2	12.000546138350089	6.823285169512906
3	4.862225447427903	4.146855118565174
4	2.494089234685187	2.8361846019799493
5	1.4332413178988366	2.0372852443565543
6	0.9274625371170839	1.5820133438510002
7	0.6157294634483753	1.2253227342993
8	0.49290120292116896	1.1210174700897333
9	0.38680817674226864	0.989693393469693
>10	3.346516461660383	20.97853479444725
>50	0.44134198717460293	8.547126316530521
>100	0.2421397656245398	12.939854664795128
>500	0.010910367346454418	2.075147384133623
>1k	0.007390894008888476	3.8453261459509838
>5k	0.0010558420012697824	2.3238849153272674
>10k+	3.519473337565941E-4	7.84994036497529
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	78456	7.807926753414773	No Hit
CCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTGCT	8894	0.8851292513621776	No Hit
CTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTGCTT	8515	0.847411240763317	Illumina Single End Adapter 1 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTGCT	5817	0.5789067748115343	No Hit
CGCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTGC	4339	0.43181648545766677	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCG	3337	0.33209762894036277	No Hit
TCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTGCT	3132	0.3116960664792377	No Hit
CGTTCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCT	2290	0.2279003806632996	No Hit
ACCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTGC	2252	0.22411862762172519	No Hit
GCCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTGC	2084	0.20739929838529095	No Hit
AGCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTGC	1888	0.18789341427611772	No Hit
ACTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTGCT	1734	0.17256736247605306	No Hit
TCCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTGC	1664	0.16560097529420545	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCT	1655	0.16470529694225364	No Hit
CGTCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTG	1562	0.1554499539720847	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTT	1547	0.15395715671883162	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTC	1527	0.1519667603811609	No Hit
TGCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTGC	1317	0.13106759883561814	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCC	1298	0.12917672231483093	No Hit
TGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTGCTTG	1290	0.12838056377976265	Illumina Single End Adapter 1 (95% over 22bp)
GGCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTGC	1263	0.12569352872390716	No Hit
ATCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTGC	1129	0.11235787326151321	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGT	1095	0.10897419948747294	No Hit
ATGCCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCT	1025	0.10200781230562536	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.8672156843231409	0.0
2	0.0	0.0	0.0	2.8610952155848035	0.0
3	0.0	0.0	0.0	4.768093946707138	0.0
4	0.0	0.0	0.0	5.9726818102654695	0.0
5	0.0	0.0	0.0	12.006966387181848	0.0
6	0.0	0.0	0.0	13.78528599507377	0.0
7	0.0	0.0	0.0	18.902196899957705	0.0
8	0.0	0.0	0.0	25.168163610578958	0.0
9	0.0	0.0	0.0	29.488716940760828	0.0
10	0.0	0.0	0.0	32.17694623441893	0.0
11	0.0	0.0	0.0	34.5244196750678	0.0
12	0.0	0.0	0.0	36.1614211429851	0.0
13	0.0	0.0	0.0	36.809991789615104	0.0
14	0.0	0.0	0.0	37.27116662105342	0.0
15	0.0	0.0	0.0	38.65041176324235	0.0
16	0.0	0.0	0.0	40.30244072350907	0.0
17	0.0	0.0	0.0	42.119174980718036	0.0
18	0.0	0.0	0.0	43.06162764660513	0.0
19	0.0	0.0	0.0	44.102604931206926	0.0
20	9.951981688353694E-5	0.0	0.0	45.18528101908292	0.0
21	1.9903963376707387E-4	0.0	0.0	46.19490956136641	0.0
22	8.956783519518324E-4	0.0	0.0	47.189610131117355	0.0
23	8.956783519518324E-4	0.0	0.0	47.94188042694002	0.0
24	0.0018908765207872017	0.0	0.0	48.564874480630955	0.0
25	0.0018908765207872017	0.0	0.0	49.16099818376334	0.0
26	0.0018908765207872017	0.0	0.0	49.73980543875799	0.0
27	0.0018908765207872017	0.0	0.0	50.305177518473364	0.0
28	0.0018908765207872017	0.0	0.0	50.860896175951034	0.0
29	0.0018908765207872017	0.0	0.0	51.549075709700695	0.0
30	0.0018908765207872017	0.0	0.0	52.152464359465576	0.0
31	0.0018908765207872017	0.0	0.0	52.65941830667032	0.0
32	0.0018908765207872017	0.0	0.0	53.18508197944916	0.0
33	0.0018908765207872017	0.0	0.0	53.69860423456821	0.0
34	0.0018908765207872017	0.0	0.0	54.30458039957207	0.0
35	0.0018908765207872017	0.0	0.0	54.75659940785709	0.0
36	0.0018908765207872017	0.0	0.0	55.19667603811609	0.0
37	0.0018908765207872017	0.0	0.0	55.6657129350882	0.0
38	0.0018908765207872017	0.0	0.0	56.11922474062648	0.0
39	0.0018908765207872017	0.0	0.0	56.57243798671411	0.0
40	0.0020899161545542755	0.0	0.0	57.032120020899164	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCTCGG	80	0.0	46.000004	1
TCCGCGG	75	0.0	46.000004	1
GCGAATA	20	6.3110725E-4	46.000004	8
TTGTCGC	35	1.01939804E-7	46.000004	37
TCGGGTA	40	5.6097633E-9	46.000004	4
CCCAGTT	20	6.3110725E-4	46.000004	18
CCAGCGG	70	0.0	46.000004	1
TTACTGA	20	6.3110725E-4	46.000004	30
GGTCACT	20	6.3110725E-4	46.000004	8
ACGGGTC	40	5.6097633E-9	46.000004	4
CTAACGG	70	0.0	46.000004	1
GAACGTT	20	6.3110725E-4	46.000004	8
GTCCGCA	20	6.3110725E-4	46.000004	30
AGTTCCG	20	6.3110725E-4	46.000004	23
ATGCGGC	40	5.6097633E-9	46.000004	2
GCTACGA	40	5.6097633E-9	46.000004	10
CCAACGG	35	1.01939804E-7	46.000004	1
AGCTACG	40	5.6097633E-9	46.000004	9
CCACGGC	20	6.3110725E-4	46.000004	2
TGTGTCA	40	5.6097633E-9	46.000004	20
>>END_MODULE