FastQCFastQC Report
Thu 26 May 2016
SRR1043371_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1043371_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences981323
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT722097.358331558518449No Hit
CCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTGC83800.8539492093836585No Hit
CTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTGCT80040.8156335885330315No Hit
GCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTGC58410.5952168653949821No Hit
CGCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTG42460.4326811865206461No Hit
TCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTGC31160.3175305174748783No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCC30520.3110087096705162No Hit
CGTTCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTC22280.22704043418935457No Hit
ACCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTG21310.21715581923586832No Hit
GCCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTG20670.21063401143150626No Hit
AGCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTG18310.1865848451529211No Hit
ACTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTGC17340.17670023019943484No Hit
TCCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTG16090.16396232433154018No Hit
CGTCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCT15310.15601387106997391No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTT15160.15448532236582654No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCT14710.14989967625338446No Hit
TGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTGCTT14460.1473520950798055Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTTCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTC14390.14663877235120343No Hit
TGCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTG13180.13430847947108138No Hit
GGCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTG12340.12574860672785618No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGC11930.12157057360318672No Hit
ATCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTG10270.10465463461062259No Hit
ATGCCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTC10210.10404321512896367No Hit
CGGCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCT10160.10353369889424786No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCG10080.1027184729187026No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCGTT253.8903865E-545.00000422
CGGGTAG600.045.0000045
GTCGCTC253.8903865E-545.00000416
GTCGCAA253.8903865E-545.0000048
CAAACGG302.1651758E-645.0000041
TACTTAG302.1651758E-645.00000422
TACTTAA302.1651758E-645.00000433
CTATCGG351.2116834E-745.0000041
CGTGCGG253.8903865E-545.0000041
CAACCGG302.1651758E-645.0000041
TTCGTCG302.1651758E-645.00000430
GCCATAC253.8903865E-545.00000412
TAGCCGT253.8903865E-545.00000420
CGTTATA253.8903865E-545.00000424
ATCGTCG253.8903865E-545.00000413
TAGCATA351.2116834E-745.00000429
ATCAACG1200.045.00000414
GTTACGG700.045.0000041
GTAGCCG253.8903865E-545.00000419
CTAAAGT302.1651758E-645.00000423