##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043371_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	981323
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.10587645454147	33.0	31.0	34.0	30.0	34.0
2	32.41016158797868	34.0	31.0	34.0	30.0	34.0
3	32.45246366384972	34.0	31.0	34.0	31.0	34.0
4	36.051037222199014	37.0	35.0	37.0	35.0	37.0
5	36.002857367044285	37.0	35.0	37.0	35.0	37.0
6	36.2621185888846	37.0	37.0	37.0	35.0	37.0
7	36.19390863151072	37.0	37.0	37.0	35.0	37.0
8	36.103219836893665	37.0	37.0	37.0	35.0	37.0
9	37.64717121681648	39.0	37.0	39.0	35.0	39.0
10	37.49525079917621	39.0	37.0	39.0	35.0	39.0
11	37.23572055276397	39.0	37.0	39.0	34.0	39.0
12	37.21287893996166	39.0	37.0	39.0	34.0	39.0
13	37.23366414524066	39.0	37.0	39.0	34.0	39.0
14	38.63018190748612	40.0	38.0	41.0	35.0	41.0
15	38.76441803565187	40.0	38.0	41.0	35.0	41.0
16	38.80667833119167	40.0	38.0	41.0	35.0	41.0
17	38.76765346374231	40.0	38.0	41.0	35.0	41.0
18	38.50083815420611	40.0	38.0	41.0	35.0	41.0
19	38.231808487113824	40.0	37.0	41.0	35.0	41.0
20	37.94138627139076	40.0	35.0	41.0	34.0	41.0
21	37.869681032646746	40.0	35.0	41.0	34.0	41.0
22	37.825124856953316	40.0	35.0	41.0	34.0	41.0
23	37.79012720582316	40.0	35.0	41.0	34.0	41.0
24	37.70496564332029	39.0	35.0	41.0	34.0	41.0
25	37.652716791515125	39.0	35.0	41.0	34.0	41.0
26	37.65591349637174	39.0	35.0	41.0	34.0	41.0
27	37.66420434454303	39.0	35.0	41.0	34.0	41.0
28	37.59914829266205	39.0	35.0	41.0	34.0	41.0
29	37.61526225310117	40.0	35.0	41.0	34.0	41.0
30	37.500875858407476	40.0	35.0	41.0	34.0	41.0
31	37.36021778761937	40.0	35.0	41.0	33.0	41.0
32	37.21378180272958	40.0	35.0	41.0	33.0	41.0
33	37.01787281048136	40.0	35.0	41.0	33.0	41.0
34	36.794520254798876	40.0	35.0	41.0	32.0	41.0
35	36.668196913758266	40.0	35.0	41.0	32.0	41.0
36	36.59170426047285	40.0	35.0	41.0	31.0	41.0
37	36.50870508487012	39.0	35.0	41.0	31.0	41.0
38	36.430228375366724	39.0	35.0	41.0	31.0	41.0
39	36.36001805725536	39.0	35.0	41.0	31.0	41.0
40	36.28570919055194	39.0	35.0	41.0	31.0	41.0
41	36.24489184498886	39.0	35.0	41.0	31.0	41.0
42	36.14418086603494	39.0	35.0	41.0	31.0	41.0
43	36.001502053859944	39.0	35.0	41.0	30.0	41.0
44	35.879730730860274	38.0	35.0	40.0	30.0	41.0
45	35.82155212911549	38.0	35.0	40.0	29.0	41.0
46	35.728776356001035	38.0	35.0	40.0	29.0	41.0
47	35.57089663647953	38.0	35.0	40.0	28.0	41.0
48	35.559854400640766	38.0	35.0	40.0	28.0	41.0
49	35.48453872985755	38.0	35.0	40.0	28.0	41.0
50	35.35454075773217	38.0	35.0	40.0	28.0	41.0
51	34.9632434988276	37.0	35.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	4.0
14	5.0
15	9.0
16	25.0
17	72.0
18	130.0
19	284.0
20	594.0
21	924.0
22	1550.0
23	2469.0
24	4345.0
25	7942.0
26	12115.0
27	14114.0
28	13773.0
29	13507.0
30	15011.0
31	17796.0
32	22452.0
33	29357.0
34	52645.0
35	98969.0
36	105433.0
37	75594.0
38	131307.0
39	360797.0
40	99.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.1494156358304	19.976093498267137	25.208111906069664	29.666378959832794
2	34.37665274328636	19.155160940893058	26.331697106864915	20.136489208955666
3	21.383988758033798	21.214421755120384	38.71345112669325	18.68813836015257
4	22.261273810967438	23.59793870112083	36.43204123412984	17.708746253781886
5	19.578467028694934	28.4220384114099	32.30577495890752	19.69371960098765
6	70.0469672065161	1.512244184636455	23.70035146429871	4.740437144548737
7	70.70465076228723	1.2590146159827091	19.4985748830915	8.537759738638552
8	57.37010138354039	2.7262175654702885	28.937363131201447	10.966317919787878
9	32.64959651409372	16.372387073369318	31.681617571380677	19.296398841156275
10	25.33508335176084	18.521424648153566	43.51981967201421	12.62367232807139
11	21.93987097010872	15.177265793219968	41.665791997130405	21.217071239540903
12	15.938890660873128	20.123649399840826	44.56106704927939	19.376392890006656
13	11.417851206993008	23.300992639528474	44.59398179804203	20.68717435543649
14	9.599591571786252	23.003129448713626	46.035199419559106	21.36207955994102
15	10.288253714628109	18.184124900771714	50.27783920279052	21.24978218180966
16	10.708910318009462	25.817595226036687	39.98561126153163	23.487883194422224
17	12.943648523472904	22.80421431068058	42.139947805156915	22.112189360689598
18	14.321074712403561	29.259886907776544	40.259119576327066	16.159918803492836
19	14.403004922945861	24.371282442172454	36.36549841387596	24.860214221005723
20	11.913508600124526	31.037385244206035	35.110458024524036	21.938648131145403
21	12.977174691717202	24.669043729740363	36.88072123042056	25.473060348121873
22	10.991997538017554	27.25392149170049	32.23597123475145	29.5181097355305
23	10.792369077255909	23.94451164397451	43.49189818235178	21.771221096417793
24	16.80720822807577	21.307051806591716	39.36369574543754	22.52204421989498
25	12.558861863015542	25.5888224366493	38.21891466927811	23.63340103105705
26	16.076969560481107	21.266800024049164	36.828241058244835	25.82798935722489
27	21.761642191205137	21.60002364155329	37.4229484074051	19.215385759836465
28	14.874103633564076	21.320401131941267	35.54028592013027	28.26520931436438
29	21.530118014150286	21.242852761017524	34.047912868647735	23.179116356184455
30	20.206496739605615	22.01629840531609	30.201880522519087	27.57532433255921
31	20.954058959180617	21.57607637852165	33.92990890868756	23.53995575361018
32	18.593368340495434	21.12719257573704	34.199035383864434	26.080403699903087
33	14.589487864851838	24.555013996410967	34.64435257300603	26.211145565731158
34	13.345453026169773	24.364047311639492	39.50340509699661	22.78709456519413
35	15.265615908319688	23.00465799741777	37.6486640993842	24.081061994878343
36	13.195349543422502	33.9352078775286	33.85572334491294	19.013719234135955
37	12.696736956129634	29.416716004822064	37.222912333655685	20.663634705392617
38	11.686977682169887	29.60360655971581	31.945546980963453	26.763868777150847
39	19.281317160608687	26.98336837106641	33.41804889929208	20.317265569032827
40	13.701197261248335	22.75163223525791	38.69307047730462	24.854100026189137
41	18.330254156888202	24.141796330056465	30.904707216686045	26.623242296369288
42	18.332597931567893	17.877294224225867	39.099970142348646	24.690137701857594
43	21.828388817952906	21.79914258608022	36.63941434165917	19.733054254307707
44	14.952161520722534	24.872646417132792	36.817846927056635	23.35734513508804
45	18.072438942122012	21.573936410335843	35.859650696050124	24.493973951492016
46	22.551290451767663	19.896405159157588	34.030895026408224	23.521409362666525
47	15.421731682636604	17.89828629309616	39.444199310522635	27.235782713744612
48	16.847358107371374	16.52279626585742	34.101921589527606	32.5279240372436
49	19.146601068149835	16.26172014718905	40.12562632283153	24.46605246182959
50	17.151539299496697	15.497343891868425	40.98803350171146	26.363083306923407
51	14.629433937653555	15.29537165642709	38.67758118376926	31.397613222150095
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	432.0
1	1723.5
2	3015.0
3	40410.5
4	77806.0
5	42140.0
6	6474.0
7	6018.5
8	5563.0
9	5927.0
10	6291.0
11	6541.5
12	6792.0
13	6600.0
14	6408.0
15	5889.0
16	5370.0
17	4860.0
18	4350.0
19	3897.0
20	3444.0
21	3233.5
22	3023.0
23	2878.5
24	2734.0
25	2695.5
26	3557.5
27	4458.0
28	4189.0
29	3920.0
30	4834.5
31	5749.0
32	6363.5
33	6978.0
34	8884.5
35	10791.0
36	12048.5
37	13306.0
38	16111.5
39	18917.0
40	26640.0
41	34363.0
42	46639.5
43	58916.0
44	86039.0
45	113162.0
46	143788.5
47	174415.0
48	169327.5
49	164240.0
50	129580.5
51	94921.0
52	73369.0
53	51817.0
54	40607.0
55	29397.0
56	24311.5
57	19226.0
58	16556.5
59	13887.0
60	11600.0
61	9313.0
62	7659.0
63	6005.0
64	4845.0
65	3685.0
66	3192.0
67	2699.0
68	2363.5
69	2028.0
70	1732.0
71	1436.0
72	1360.5
73	1285.0
74	1086.0
75	717.5
76	548.0
77	439.0
78	330.0
79	241.0
80	152.0
81	103.0
82	54.0
83	49.0
84	44.0
85	27.5
86	11.0
87	8.0
88	5.0
89	9.5
90	14.0
91	8.0
92	2.0
93	2.0
94	2.0
95	1.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	981323.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.089903977405427
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.28607520010843	20.305089112745538
2	12.0691774939122	6.780440737805127
3	4.900210979283835	4.129393676313322
4	2.457197821870653	2.760898034793456
5	1.5205236713507904	2.1355681961807833
6	0.9789157533902474	1.6498589708820925
7	0.6728404543992381	1.3230016629331902
8	0.4961911017180883	1.1150368321363286
9	0.4094365813560613	1.0350930829616016
>10	3.496612722150273	21.538583430902115
>50	0.4389671584994415	8.333350642458036
>100	0.2533488434182251	13.229368615633167
>500	0.01134944240746381	2.1235481385588373
>1k	0.007688331953443225	3.8291535372705576
>5k	0.0010983331362061752	2.28562435853892
>10k+	3.661110454020584E-4	7.425990969886924
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	72209	7.358331558518449	No Hit
CCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTGC	8380	0.8539492093836585	No Hit
CTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTGCT	8004	0.8156335885330315	No Hit
GCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTGC	5841	0.5952168653949821	No Hit
CGCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTG	4246	0.4326811865206461	No Hit
TCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTGC	3116	0.3175305174748783	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCC	3052	0.3110087096705162	No Hit
CGTTCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTC	2228	0.22704043418935457	No Hit
ACCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTG	2131	0.21715581923586832	No Hit
GCCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTG	2067	0.21063401143150626	No Hit
AGCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTG	1831	0.1865848451529211	No Hit
ACTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTGC	1734	0.17670023019943484	No Hit
TCCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTG	1609	0.16396232433154018	No Hit
CGTCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCT	1531	0.15601387106997391	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTT	1516	0.15448532236582654	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCT	1471	0.14989967625338446	No Hit
TGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTGCTT	1446	0.1473520950798055	Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTTCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTC	1439	0.14663877235120343	No Hit
TGCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTG	1318	0.13430847947108138	No Hit
GGCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTG	1234	0.12574860672785618	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGC	1193	0.12157057360318672	No Hit
ATCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTG	1027	0.10465463461062259	No Hit
ATGCCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTC	1021	0.10404321512896367	No Hit
CGGCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCT	1016	0.10353369889424786	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCG	1008	0.1027184729187026	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.8385618190952419	0.0
2	0.0	0.0	0.0	2.832706458525888	0.0
3	0.0	0.0	0.0	4.745634210142838	0.0
4	0.0	0.0	0.0	5.9798863371183595	0.0
5	0.0	0.0	0.0	12.09662873488138	0.0
6	0.0	0.0	0.0	13.904901851887706	0.0
7	0.0	0.0	0.0	19.06864508423832	0.0
8	0.0	0.0	0.0	25.525132907309825	0.0
9	0.0	0.0	0.0	30.05085991054933	0.0
10	0.0	0.0	0.0	32.835468036518044	0.0
11	0.0	0.0	0.0	35.244358891007344	0.0
12	0.0	0.0	0.0	36.90996746229325	0.0
13	0.0	0.0	0.0	37.572949986905435	0.0
14	0.0	0.0	0.0	38.02234330592476	0.0
15	0.0	0.0	0.0	39.48801770670819	0.0
16	0.0	0.0	0.0	41.185114381299535	0.0
17	0.0	0.0	0.0	43.09141842186518	0.0
18	0.0	0.0	0.0	44.063473494455955	0.0
19	0.0	0.0	0.0	45.13417091008771	0.0
20	0.0	0.0	0.0	46.23401265434521	0.0
21	1.0190324694315735E-4	0.0	0.0	47.280253290710604	0.0
22	6.11419481658944E-4	0.0	0.0	48.3116160530223	0.0
23	6.11419481658944E-4	0.0	0.0	49.09148160187828	0.0
24	0.0010190324694315735	0.0	0.0	49.737038671263186	0.0
25	0.0010190324694315735	0.0	0.0	50.33806402173393	0.0
26	0.0010190324694315735	0.0	0.0	50.93470753258611	0.0
27	0.0010190324694315735	0.0	0.0	51.50506000572696	0.0
28	0.0011209357163747308	0.0	0.0	52.097627386701426	0.0
29	0.0011209357163747308	0.0	0.0	52.803307371782786	0.0
30	0.0011209357163747308	0.0	0.0	53.40504604498213	0.0
31	0.0011209357163747308	0.0	0.0	53.92852302452913	0.0
32	0.0011209357163747308	0.0	0.0	54.46473790994403	0.0
33	0.0011209357163747308	0.0	0.0	54.97486556414147	0.0
34	0.0011209357163747308	0.0	0.0	55.58669265878819	0.0
35	0.0011209357163747308	0.0	0.0	56.053613336281735	0.0
36	0.0011209357163747308	0.0	0.0	56.51391030272398	0.0
37	0.0011209357163747308	0.0	0.0	56.981544302946126	0.0
38	0.0011209357163747308	0.0	0.0	57.443777431080285	0.0
39	0.0011209357163747308	0.0	0.0	57.9044820105103	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTCGTT	25	3.8903865E-5	45.000004	22
CGGGTAG	60	0.0	45.000004	5
GTCGCTC	25	3.8903865E-5	45.000004	16
GTCGCAA	25	3.8903865E-5	45.000004	8
CAAACGG	30	2.1651758E-6	45.000004	1
TACTTAG	30	2.1651758E-6	45.000004	22
TACTTAA	30	2.1651758E-6	45.000004	33
CTATCGG	35	1.2116834E-7	45.000004	1
CGTGCGG	25	3.8903865E-5	45.000004	1
CAACCGG	30	2.1651758E-6	45.000004	1
TTCGTCG	30	2.1651758E-6	45.000004	30
GCCATAC	25	3.8903865E-5	45.000004	12
TAGCCGT	25	3.8903865E-5	45.000004	20
CGTTATA	25	3.8903865E-5	45.000004	24
ATCGTCG	25	3.8903865E-5	45.000004	13
TAGCATA	35	1.2116834E-7	45.000004	29
ATCAACG	120	0.0	45.000004	14
GTTACGG	70	0.0	45.000004	1
GTAGCCG	25	3.8903865E-5	45.000004	19
CTAAAGT	30	2.1651758E-6	45.000004	23
>>END_MODULE