FastQCFastQC Report
Thu 26 May 2016
SRR1043370_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1043370_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences495841
Sequences flagged as poor quality0
Sequence length52
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT273025.506200576394448No Hit
CCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTGCT44900.9055322169808467No Hit
CTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTGCTT41430.8355501057798771Illumina Single End Adapter 2 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTGCT30890.6229819639763553No Hit
TCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTGCT15610.3148186616274169No Hit
CGCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTGC15440.311390143211231No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCG12970.2615757873995898No Hit
ACCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTGC12500.2520969423666054No Hit
GCCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTGC10520.21216478669573513No Hit
AGCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTGC9500.1915936761986201No Hit
ACTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTGCT8860.17868631274944993No Hit
TCCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTGC8280.16698901462363944No Hit
CGGCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTG7940.16013197779126775No Hit
CGTTCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCT7510.15145984297385653No Hit
TGCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTGC6750.13613234887796694No Hit
ATCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTGC6200.12504008341383627No Hit
ATTGCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCT5670.11435117305749222No Hit
AGAGCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCT5490.1107209770874131No Hit
GGCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTGC5290.10668742600954742No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCC5190.10467065047061457No Hit
ATAGCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCT5120.10325890759336157No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCT5110.10305723003946829No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5100.10285555248557503No Hit
GTCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTGC4970.10023374428496232No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTGGGA750.046.0000043
TATGAGG551.8189894E-1246.0000041
TGATCGG253.4143253E-546.01
GTGTTGT206.307965E-446.037
GTGCATG206.307965E-446.032
ACAACAG253.4143253E-546.019
CGGGTAA206.307965E-446.05
GTACCGG253.4143253E-546.01
CAAACGG206.307965E-446.01
CCCTCCG206.307965E-446.022
GTCGACT253.4143253E-546.033
GTACACT253.4143253E-546.017
TTGTGCA206.307965E-446.030
CTCCGGG600.046.02
GCGGTCC206.307965E-446.010
CTATCAA206.307965E-446.043
GCGAAGC301.8592473E-646.016
TCCTGAC206.307965E-446.014
GCAACTC206.307965E-446.045
ACCGCGA206.307965E-446.023
Produced by FastQC (version 0.11.5)