##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043370_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	495841
Sequences flagged as poor quality	0
Sequence length	52
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.23763464497692	33.0	31.0	34.0	30.0	34.0
2	32.55347379502704	34.0	31.0	34.0	31.0	34.0
3	32.621398795178294	34.0	31.0	34.0	31.0	34.0
4	36.12299305624182	37.0	35.0	37.0	35.0	37.0
5	36.009129942864746	37.0	35.0	37.0	35.0	37.0
6	36.274442815337984	37.0	37.0	37.0	35.0	37.0
7	36.19106729778296	37.0	37.0	37.0	35.0	37.0
8	36.17279329462469	37.0	37.0	37.0	35.0	37.0
9	37.76507590134741	39.0	38.0	39.0	35.0	39.0
10	37.58485482241283	39.0	37.0	39.0	35.0	39.0
11	37.13259290780714	39.0	35.0	39.0	34.0	39.0
12	36.99911866908948	39.0	35.0	39.0	33.0	39.0
13	37.0289467793103	39.0	35.0	39.0	33.0	39.0
14	38.421431466942025	40.0	37.0	41.0	34.0	41.0
15	38.54691524097443	40.0	37.0	41.0	35.0	41.0
16	38.55392151919668	40.0	37.0	41.0	35.0	41.0
17	38.56165585338849	40.0	37.0	41.0	35.0	41.0
18	38.312122232731866	40.0	36.0	41.0	35.0	41.0
19	38.05532015303293	40.0	36.0	41.0	34.0	41.0
20	37.723867126760396	39.0	35.0	41.0	34.0	41.0
21	37.67365949971866	39.0	35.0	41.0	34.0	41.0
22	37.64822392662164	39.0	35.0	41.0	34.0	41.0
23	37.63232568504823	39.0	35.0	41.0	34.0	41.0
24	37.53564348248733	39.0	35.0	41.0	34.0	41.0
25	37.44515681438203	39.0	35.0	41.0	34.0	41.0
26	37.37931716013803	39.0	35.0	41.0	34.0	41.0
27	37.39502985836185	39.0	35.0	41.0	34.0	41.0
28	37.37980118626737	39.0	35.0	41.0	33.0	41.0
29	37.2396695714957	39.0	35.0	41.0	33.0	41.0
30	37.09048061777868	39.0	35.0	41.0	33.0	41.0
31	36.988163544362	39.0	35.0	41.0	33.0	41.0
32	36.678324301540215	39.0	35.0	41.0	32.0	41.0
33	36.48546610707868	39.0	35.0	41.0	31.0	41.0
34	36.32120780653476	39.0	35.0	41.0	31.0	41.0
35	36.17810548139424	39.0	35.0	41.0	31.0	41.0
36	36.15209512726862	39.0	35.0	41.0	30.0	41.0
37	36.153633927004826	39.0	35.0	41.0	31.0	41.0
38	36.13361742978092	39.0	35.0	41.0	31.0	41.0
39	36.11312295675428	39.0	35.0	41.0	30.0	41.0
40	36.0640003549525	39.0	35.0	41.0	31.0	41.0
41	36.015303292789426	38.0	35.0	41.0	31.0	41.0
42	35.87001881651578	38.0	35.0	41.0	30.0	41.0
43	35.734959392224525	38.0	35.0	41.0	29.0	41.0
44	35.547689682781375	38.0	35.0	40.0	28.0	41.0
45	35.4571162933279	38.0	35.0	40.0	28.0	41.0
46	35.410357352457744	38.0	35.0	40.0	28.0	41.0
47	35.24653265865469	37.0	35.0	40.0	28.0	41.0
48	35.11106584570457	37.0	35.0	40.0	28.0	41.0
49	34.85583886770154	37.0	35.0	39.0	28.0	41.0
50	34.58426592395546	36.0	35.0	39.0	27.0	40.0
51	34.429839404163836	36.0	35.0	39.0	27.0	40.0
52	34.00730476100202	36.0	34.0	39.0	26.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	2.0
15	3.0
16	14.0
17	30.0
18	76.0
19	180.0
20	323.0
21	570.0
22	980.0
23	1711.0
24	2964.0
25	5141.0
26	6962.0
27	7530.0
28	7040.0
29	7150.0
30	8011.0
31	9681.0
32	11977.0
33	15825.0
34	29543.0
35	63933.0
36	46747.0
37	37892.0
38	68511.0
39	162901.0
40	143.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.24349337791752	20.36600442480553	24.360631734769818	29.029870462507134
2	32.30531561528797	20.407348323353656	26.96933089437945	20.31800516697893
3	21.585145238090437	23.15802848090416	37.34806117283564	17.908765108169757
4	23.58457650738846	24.58187201139075	33.969962145123134	17.86358933609766
5	18.862901615638883	29.99993949673383	30.561813161880526	20.57534572574676
6	71.95814787401606	1.662629754296236	22.006247970619615	4.372974401068085
7	72.34819226324568	1.6360083171823228	17.313816324184568	8.701983095387433
8	58.709949358766224	3.284117287598242	27.366635675549215	10.639297678086322
9	34.73109323351639	18.74492024661131	28.50087023864505	18.023116281227246
10	28.306049721584138	18.091686649550965	42.25850625502933	11.343757373835563
11	25.233290510466055	14.767032173620173	38.89492801119714	21.104749304716634
12	15.653203345427263	24.409236025258096	42.690902930576534	17.246657698738105
13	10.493686484175372	28.80661341034727	41.80513511387723	18.89456499160013
14	8.998247422056666	22.476963381406538	49.736508275838425	18.78828092069837
15	9.123489183024397	17.46023422831109	52.42829858765209	20.987978001012422
16	9.195286392210406	24.394916918931674	38.31207988044555	28.097716808412375
17	12.522562676341812	21.970551043580503	42.58824905564486	22.918637224432832
18	15.77582329819438	29.270068429194033	38.783803679001934	16.170304593609643
19	16.52626547623129	22.639515489844527	34.99871934753278	25.835499686391405
20	10.80749675803332	31.651476985565935	35.506341750682175	22.034684505718566
21	13.187291893974077	23.94396590842629	35.070516556718786	27.798225640880847
22	10.008046934400342	28.23626928793706	31.159787109174108	30.595896668488486
23	10.119372944149434	23.295975927767167	44.863373541114996	21.721277586968405
24	15.856090964643906	21.469382322155692	39.85047626154352	22.824050451656884
25	11.830606988933953	27.514465322552994	37.29360823328446	23.36131945522859
26	16.61399521217487	23.71909543583528	34.76941196875612	24.897497383233738
27	23.691465610951898	22.50277810830488	37.90005263784156	15.905703642901656
28	17.291026760594626	23.137860725514834	32.59613464800208	26.974977865888462
29	23.95747830453714	24.908993003805655	29.19242257094512	21.941106120712085
30	29.201094705762532	24.130921000885365	25.755232019941875	20.912752273410227
31	31.187416934057488	21.569011033778974	26.921331636552846	20.322240395610688
32	26.808190528818717	28.18463983414038	26.26587958639967	18.741290050641236
33	26.021849746188796	25.12035108028582	29.717187566175447	19.140611607349935
34	23.45328441980393	22.373704473813177	35.37787314885215	18.79513795753074
35	24.942874832859726	32.15002389879014	28.013415590884982	14.893685677465154
36	19.529244253702295	29.29910999695467	36.409252159462405	14.762393589880626
37	17.371496104598048	30.709239453776515	37.45091672532122	14.46834771630422
38	14.68091585810774	29.039954340201803	35.630978478988226	20.648151322702237
39	22.654842983940416	23.331269499698493	38.06744500757299	15.9464425087881
40	15.391425880473781	18.477294132594924	42.70965894308861	23.421621043842684
41	23.16347377485928	21.84571263772056	30.97787395556237	24.012939631857794
42	22.82263870877963	17.68006276205477	36.981209702303765	22.51608882686184
43	25.54326891080003	23.171540877015012	33.36109760991931	17.924092602265645
44	16.070070849324683	28.487156164980306	33.67006762248382	21.77270536321119
45	20.623546661127257	24.32836332614689	33.34677850359288	21.701311509132967
46	24.988252282485714	19.68312422732287	34.062532142360155	21.26609134783126
47	17.584064246401567	18.668282776131864	39.16759606406085	24.58005691340571
48	19.03392418134039	14.919097049255708	34.38985481232895	31.65712395707495
49	20.756250491589036	17.699020452120738	39.54352302451794	22.001206031772284
50	18.320792350773736	16.7152373442293	39.253712379573294	25.710257925423676
51	16.73742187515756	14.48448192061568	38.64444448926168	30.133651714965083
52	15.820394037604796	14.475809785798269	47.564642697961645	22.139153478635286
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	255.0
1	852.0
2	1449.0
3	15260.5
4	29072.0
5	15830.0
6	2588.0
7	2637.0
8	2686.0
9	2955.5
10	3225.0
11	3360.0
12	3495.0
13	3505.0
14	3295.0
15	3075.0
16	2783.0
17	2491.0
18	2263.0
19	2035.0
20	1921.0
21	1807.0
22	1671.0
23	1535.0
24	1509.0
25	1483.0
26	1858.0
27	2233.0
28	2091.5
29	1950.0
30	2003.5
31	2057.0
32	2583.0
33	3109.0
34	3760.5
35	4412.0
36	5092.5
37	5773.0
38	6856.5
39	10969.5
40	13999.0
41	20404.0
42	26809.0
43	42173.5
44	57538.0
45	71270.0
46	85002.0
47	85470.5
48	85939.0
49	70780.5
50	55622.0
51	42891.0
52	30160.0
53	23560.5
54	16961.0
55	13796.5
56	10632.0
57	9392.0
58	8152.0
59	7065.5
60	5979.0
61	4851.5
62	3724.0
63	3177.0
64	2163.0
65	1696.0
66	1482.5
67	1269.0
68	1131.0
69	993.0
70	893.5
71	794.0
72	806.0
73	818.0
74	628.5
75	439.0
76	364.0
77	289.0
78	197.5
79	106.0
80	83.0
81	60.0
82	42.0
83	24.0
84	18.5
85	13.0
86	10.5
87	8.0
88	4.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	495841.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.87776912859045
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.99537136140817	21.079434816304687
2	11.183020038796924	6.4588134168155635
3	4.673637552235451	4.048926786724978
4	2.424750616940348	2.8008555404164257
5	1.4481165436717507	2.0909187609722593
6	0.9897190691954376	1.7148527269433573
7	0.692632427123108	1.4001175525005456
8	0.5225852297786507	1.2072876492447415
9	0.4166867328662202	1.0829684943591613
>10	3.8524655470530265	25.147944902694174
>50	0.5720534595459353	11.074823136419788
>100	0.21285710122639456	10.786487895922187
>500	0.009802629661741854	1.8604295566749154
>1k	0.005601502663852488	3.7257118803708287
>5k	0.0	0.0
>10k+	7.00187832981561E-4	5.520426883636403
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	27302	5.506200576394448	No Hit
CCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTGCT	4490	0.9055322169808467	No Hit
CTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTGCTT	4143	0.8355501057798771	Illumina Single End Adapter 2 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTGCT	3089	0.6229819639763553	No Hit
TCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTGCT	1561	0.3148186616274169	No Hit
CGCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTGC	1544	0.311390143211231	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCG	1297	0.2615757873995898	No Hit
ACCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTGC	1250	0.2520969423666054	No Hit
GCCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTGC	1052	0.21216478669573513	No Hit
AGCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTGC	950	0.1915936761986201	No Hit
ACTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTGCT	886	0.17868631274944993	No Hit
TCCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTGC	828	0.16698901462363944	No Hit
CGGCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTG	794	0.16013197779126775	No Hit
CGTTCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCT	751	0.15145984297385653	No Hit
TGCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTGC	675	0.13613234887796694	No Hit
ATCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTGC	620	0.12504008341383627	No Hit
ATTGCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCT	567	0.11435117305749222	No Hit
AGAGCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCT	549	0.1107209770874131	No Hit
GGCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTGC	529	0.10668742600954742	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCC	519	0.10467065047061457	No Hit
ATAGCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCT	512	0.10325890759336157	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCT	511	0.10305723003946829	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	510	0.10285555248557503	No Hit
GTCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTGC	497	0.10023374428496232	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.8585413469237114	0.0
2	0.0	0.0	0.0	2.9388453153329395	0.0
3	0.0	0.0	0.0	4.813034823663231	0.0
4	0.0	0.0	0.0	6.016444787744458	0.0
5	0.0	0.0	0.0	12.289625101595067	0.0
6	0.0	0.0	0.0	13.675956607057504	0.0
7	0.0	0.0	0.0	18.935303857486574	0.0
8	0.0	0.0	0.0	25.13709031725896	0.0
9	0.0	0.0	0.0	29.61413033613598	0.0
10	0.0	0.0	0.0	32.06874784457114	0.0
11	0.0	0.0	0.0	34.471937576763516	0.0
12	0.0	0.0	0.0	36.27251477792276	0.0
13	0.0	0.0	0.0	36.93099199138433	0.0
14	0.0	0.0	0.0	37.38940507138377	0.0
15	0.0	0.0	0.0	39.01855635173372	0.0
16	0.0	0.0	0.0	40.82256207130915	0.0
17	0.0	0.0	0.0	43.428437745164274	0.0
18	0.0	0.0	0.0	44.3827759301873	0.0
19	0.0	0.0	0.0	45.50692661558846	0.0
20	0.0	0.0	0.0	46.51712948303992	0.0
21	0.0	0.0	0.0	47.37002385845462	0.0
22	0.0020167755389328434	0.0	0.0	48.31266474535184	0.0
23	0.002218453092826128	0.0	0.0	48.993729844849454	0.0
24	0.004436906185652256	0.0	0.0	49.5178898074181	0.0
25	0.005243616401225393	0.0	0.0	50.02773066366033	0.0
26	0.005243616401225393	0.0	0.0	50.52970609530071	0.0
27	0.005848649062905246	0.0	0.0	51.0226060370159	0.0
28	0.006655359278478383	0.0	0.0	51.52074959513231	0.0
29	0.006655359278478383	0.0	0.0	52.17035299622258	0.0
30	0.006655359278478383	0.0	0.0	52.73464679201599	0.0
31	0.006655359278478383	0.0	0.0	53.1218676954911	0.0
32	0.006655359278478383	0.0	0.0	53.54095365248134	0.0
33	0.006655359278478383	0.0	0.0	53.973350328028545	0.0
34	0.006655359278478383	0.0	0.0	54.58524002654077	0.0
35	0.006655359278478383	0.0	0.0	55.00493101619269	0.0
36	0.006655359278478383	0.0	0.0	55.38710998082046	0.0
37	0.006655359278478383	0.0	0.0	55.76868391278656	0.0
38	0.006655359278478383	0.0	0.0	56.180307800282755	0.0
39	0.006655359278478383	0.0	0.0	56.5812427774226	0.0
40	0.006655359278478383	0.0	0.0	56.988026403625355	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGTGGGA	75	0.0	46.000004	3
TATGAGG	55	1.8189894E-12	46.000004	1
TGATCGG	25	3.4143253E-5	46.0	1
GTGTTGT	20	6.307965E-4	46.0	37
GTGCATG	20	6.307965E-4	46.0	32
ACAACAG	25	3.4143253E-5	46.0	19
CGGGTAA	20	6.307965E-4	46.0	5
GTACCGG	25	3.4143253E-5	46.0	1
CAAACGG	20	6.307965E-4	46.0	1
CCCTCCG	20	6.307965E-4	46.0	22
GTCGACT	25	3.4143253E-5	46.0	33
GTACACT	25	3.4143253E-5	46.0	17
TTGTGCA	20	6.307965E-4	46.0	30
CTCCGGG	60	0.0	46.0	2
GCGGTCC	20	6.307965E-4	46.0	10
CTATCAA	20	6.307965E-4	46.0	43
GCGAAGC	30	1.8592473E-6	46.0	16
TCCTGAC	20	6.307965E-4	46.0	14
GCAACTC	20	6.307965E-4	46.0	45
ACCGCGA	20	6.307965E-4	46.0	23
>>END_MODULE