SRR1043369_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1043369_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	494776
Sequences flagged as poor quality	0
Sequence length	51
%GC	43

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	25979	5.250658884020243	No Hit
CCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTGC	4432	0.8957588888709234	No Hit
CTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTGCT	3991	0.8066276456416641	No Hit
GCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTGC	3280	0.6629262534965318	No Hit
CGCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTG	1558	0.3148899704108526	No Hit
TCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTGC	1482	0.2995294840493476	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCC	1265	0.2556712532539978	No Hit
ACCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTG	1244	0.25142690833831877	No Hit
GCCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTG	1136	0.22959884877196954	No Hit
AGCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTG	1008	0.20372855595259268	No Hit
ACTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTGC	847	0.17118857826572026	No Hit
CGGCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCT	818	0.16532734004883018	No Hit
TCCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTG	792	0.16007243681989425	No Hit
TGCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTG	696	0.14066971720536162	No Hit
CGTTCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTC	693	0.14006338221740747	No Hit
AGAGCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTC	626	0.12652190081976492	No Hit
ATCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTG	605	0.12227755590408589	No Hit
ATTGCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTC	565	0.11419308939803062	No Hit
TGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTGCTT	533	0.1077255161931864	Illumina Single End Adapter 2 (95% over 21bp)
GGCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTG	525	0.10610862289197535	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	515	0.10408750626546154	No Hit
AGACCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTC	515	0.10408750626546154	No Hit
AGCTACCATTACATTTATTATTTTACTTCTACTCACAATTCTAGAATTTGC	498	0.10065160800038805	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTGTTC	30	2.1629858E-6	45.000004	14
ACAACGA	30	2.1629858E-6	45.000004	33
GTTTGCG	25	3.887573E-5	45.000004	17
CGGGTAC	30	2.1629858E-6	45.000004	5
TCTGATT	30	2.1629858E-6	45.000004	25
CCTTAGG	30	2.1629858E-6	45.000004	1
CTGTCGG	25	3.887573E-5	45.000004	1
AGTCAGG	35	1.2100463E-7	45.000004	1
GTCGACT	25	3.887573E-5	45.000004	33
TCCGATT	30	2.1629858E-6	45.000004	41
CGGCCAT	25	3.887573E-5	45.000004	7
AACCATG	25	3.887573E-5	45.000004	10
CATGACA	25	3.887573E-5	45.000004	43
TGGGTAC	25	3.887573E-5	45.000004	5
GTCTCAC	70	0.0	45.000004	21
ACCTGGT	25	3.887573E-5	45.000004	10
TCGGGCA	25	3.887573E-5	45.000004	4
TATATGG	25	3.887573E-5	45.000004	1
ATGCTGG	30	2.1629858E-6	45.000004	1
TTGTAGC	35	1.2100463E-7	45.000004	1

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content