##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043369_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	494776
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.158180671657476	33.0	31.0	34.0	30.0	34.0
2	32.4393139521723	34.0	31.0	34.0	31.0	34.0
3	32.505058854916165	34.0	31.0	34.0	31.0	34.0
4	36.06690300257086	37.0	35.0	37.0	35.0	37.0
5	36.002241418338805	37.0	35.0	37.0	35.0	37.0
6	36.2702313774314	37.0	37.0	37.0	35.0	37.0
7	36.195658237262926	37.0	37.0	37.0	35.0	37.0
8	36.09703583035555	37.0	37.0	37.0	35.0	37.0
9	37.69336629100846	39.0	38.0	39.0	35.0	39.0
10	37.523406551651654	39.0	37.0	39.0	35.0	39.0
11	37.1213943279383	39.0	35.0	39.0	34.0	39.0
12	36.995317072776366	39.0	35.0	39.0	33.0	39.0
13	37.025983475350465	39.0	35.0	39.0	33.0	39.0
14	38.41931096091969	40.0	37.0	41.0	34.0	41.0
15	38.561049848820474	40.0	37.0	41.0	35.0	41.0
16	38.59615664462302	40.0	37.0	41.0	35.0	41.0
17	38.590509644768545	40.0	37.0	41.0	35.0	41.0
18	38.37019378466215	40.0	37.0	41.0	35.0	41.0
19	38.1284682361311	40.0	37.0	41.0	34.0	41.0
20	37.82108065063787	40.0	35.0	41.0	34.0	41.0
21	37.7699565055702	39.0	35.0	41.0	34.0	41.0
22	37.75319942761977	39.0	35.0	41.0	34.0	41.0
23	37.71619682442156	39.0	35.0	41.0	34.0	41.0
24	37.61648907788575	39.0	35.0	41.0	34.0	41.0
25	37.60188448914256	39.0	35.0	41.0	34.0	41.0
26	37.57307953498149	39.0	35.0	41.0	34.0	41.0
27	37.559477420085045	39.0	35.0	41.0	34.0	41.0
28	37.532418710689285	39.0	35.0	41.0	34.0	41.0
29	37.41764151858619	39.0	35.0	41.0	34.0	41.0
30	37.20435712322344	39.0	35.0	41.0	33.0	41.0
31	37.1039379436351	39.0	35.0	41.0	33.0	41.0
32	36.806932834252265	39.0	35.0	41.0	32.0	41.0
33	36.57969262858344	39.0	35.0	41.0	31.0	41.0
34	36.36257215386357	39.0	35.0	41.0	31.0	41.0
35	36.20727561563213	39.0	35.0	41.0	31.0	41.0
36	36.17129771856355	39.0	35.0	41.0	31.0	41.0
37	36.119825941436126	39.0	35.0	41.0	30.0	41.0
38	36.09524714214109	39.0	35.0	41.0	30.0	41.0
39	36.034534819797244	38.0	35.0	41.0	30.0	41.0
40	35.96246584312901	38.0	35.0	41.0	30.0	41.0
41	35.873944977120956	38.0	35.0	41.0	30.0	41.0
42	35.71088937216033	38.0	35.0	40.0	29.0	41.0
43	35.561896696686986	38.0	35.0	40.0	28.0	41.0
44	35.410511019127846	38.0	35.0	40.0	28.0	41.0
45	35.34942074797484	38.0	35.0	40.0	28.0	41.0
46	35.23990250133394	37.0	35.0	40.0	28.0	41.0
47	35.015724287354274	37.0	35.0	40.0	27.0	41.0
48	34.92498221417369	37.0	35.0	40.0	27.0	41.0
49	34.66554764176112	36.0	35.0	39.0	27.0	40.0
50	34.43071814315973	36.0	35.0	39.0	26.0	40.0
51	33.98259414361247	36.0	34.0	39.0	25.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	0.0
12	0.0
13	0.0
14	4.0
15	4.0
16	10.0
17	25.0
18	65.0
19	167.0
20	286.0
21	525.0
22	908.0
23	1424.0
24	2366.0
25	4511.0
26	6615.0
27	7784.0
28	7468.0
29	7334.0
30	8348.0
31	9850.0
32	12011.0
33	15970.0
34	30234.0
35	62030.0
36	45212.0
37	38338.0
38	71457.0
39	161819.0
40	10.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.662368425307616	20.475326208223517	24.47026533219073	28.39204003427814
2	32.17233657250958	20.341124064223003	27.058911507429624	20.427627855837795
3	21.664349119601596	23.32954710818633	36.94439503937135	18.06170873284072
4	23.67455171633224	24.814865717011333	33.621072970394685	17.889509596261743
5	19.16321729429075	30.14798615939334	30.092203340501563	20.596593205814347
6	72.4042799165683	1.5906187850663736	21.585121347842257	4.419979950523064
7	72.75777321454558	1.598299028247126	16.88582307953498	8.758104677672321
8	58.917772891166905	3.225095800928097	27.05143337591152	10.805697931993468
9	34.70580626384464	18.8412534156871	28.161632738855563	18.29130758161269
10	28.407602632302293	18.099099390431224	42.15079146927094	11.342506507995537
11	25.400181092049735	14.592462043429755	38.74015716202888	21.26719970249163
12	15.974097369314599	24.1082833443821	42.59361812214012	17.324001164163178
13	10.591863792908306	28.62931912623086	41.59660129028085	19.182215790579978
14	9.003872459456401	22.418831956279206	49.56000291040794	19.017292673856453
15	9.05197503516743	17.440619593513023	52.181391174996364	21.326014196323186
16	9.19688909728847	24.32555338173234	38.337752841689976	28.139804679289217
17	12.712823580771904	21.911127459698935	42.36401927336815	23.01202968616101
18	15.917708215434864	29.266375086908013	38.70701084935405	16.10890584830307
19	16.51939463514803	22.677736996135625	34.67043672288066	26.13243164583569
20	10.860065969246689	31.755986547447733	35.1961291574369	22.187818325868676
21	13.152214335376009	23.905363235080117	34.72884699338691	28.21357543615697
22	10.018675117628987	28.247934418807702	30.892969747926337	30.840420715636974
23	10.131251313725807	23.201408314065354	44.610490403738254	22.05684996847058
24	15.953481979724158	21.457588888709232	39.63975617249017	22.94917295907643
25	11.744506605009136	27.366323346322375	37.33063042669814	23.558539621970347
26	16.63419405953401	23.523170081006352	34.63809885685644	25.2045370026032
27	23.933052532863357	22.306659983507686	37.69342086115737	16.066866622471583
28	17.494785519103594	23.239809529969115	32.11817064691901	27.14723430400828
29	24.169523178165473	24.740488625155628	28.82031464743642	22.269673549242487
30	29.387439972836194	24.050883632189112	25.409680340194353	21.151996054780344
31	31.407950264362057	21.47335359839604	26.49057351205394	20.628122625187963
32	27.14602163403237	27.821276698950637	25.959221950943455	19.073479716073535
33	26.595671576632657	24.748775203324332	29.34439018869145	19.311163031351562
34	24.088072178116963	22.171851504519218	35.0435348521351	18.69654146522871
35	25.20190955098873	32.21619480330493	27.690308341552537	14.8915873041538
36	19.72084337154591	29.51315342700535	35.968599932090484	14.797403269358256
37	17.62454120652578	30.61910844503371	37.29748411402332	14.458866234417192
38	14.837825601888532	28.946432324930875	35.43542936601614	20.780312707164455
39	22.775559040858894	23.30590004365612	38.05115850405032	15.867382411434669
40	15.667898200397756	18.272915420311413	42.81674939770724	23.242436981583587
41	23.528222872572638	21.56531440490242	30.89782042782997	24.008642294694972
42	23.043559105534626	17.50549743722412	36.717423642213845	22.733519815027407
43	25.922437628340905	23.013646579462222	33.10973046388669	17.954185328310185
44	16.199856096496195	28.187907255000244	33.71141688359985	21.900819764903716
45	20.69320258056171	24.202871602502952	33.38824033502029	21.71568548191505
46	25.32034698530244	19.364116286966222	34.095226930974825	21.220309796756514
47	17.8763723381894	18.443093440263876	39.094256794994095	24.586277426552623
48	19.036493281808333	14.704027681213317	34.293296360373176	31.96618267660517
49	20.96524487849047	17.39049590117548	39.408338318754346	22.235920901579707
50	18.619536921758534	16.419551473798244	39.211077336006596	25.74983426843663
51	16.86338868498068	14.396817953983215	38.46387051918444	30.275922841851667
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	181.0
1	805.5
2	1430.0
3	14573.5
4	27717.0
5	15243.0
6	2769.0
7	2716.0
8	2663.0
9	3023.0
10	3383.0
11	3518.5
12	3654.0
13	3504.0
14	3354.0
15	3057.5
16	2761.0
17	2502.0
18	2243.0
19	2015.0
20	1787.0
21	1678.5
22	1570.0
23	1436.0
24	1302.0
25	1335.0
26	1699.0
27	2030.0
28	1961.5
29	1893.0
30	2042.0
31	2191.0
32	2872.0
33	3553.0
34	4026.0
35	4499.0
36	5439.0
37	6379.0
38	8130.0
39	9881.0
40	14514.5
41	19148.0
42	26981.5
43	34815.0
44	49978.5
45	65142.0
46	78679.5
47	92217.0
48	87704.5
49	83192.0
50	63837.0
51	44482.0
52	34343.0
53	24204.0
54	18754.5
55	13305.0
56	11358.5
57	9412.0
58	8229.5
59	7047.0
60	5930.5
61	4814.0
62	3765.0
63	2716.0
64	2426.0
65	2136.0
66	1878.0
67	1620.0
68	1290.5
69	961.0
70	938.5
71	916.0
72	914.0
73	912.0
74	737.0
75	425.0
76	288.0
77	225.5
78	163.0
79	115.5
80	68.0
81	45.0
82	22.0
83	17.0
84	12.0
85	13.0
86	14.0
87	7.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	494776.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.926148397841107
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.89378833363524	21.085365386195516
2	11.294770315792949	6.534284045483149
3	4.656243646999884	4.040615841288705
4	2.4302979016390016	2.8119663101508645
5	1.4597155716275674	2.1111974621769236
6	0.9505734050310624	1.6497856426181778
7	0.6857570001061724	1.3885416124950634
8	0.5219867277617818	1.2079252439152628
9	0.3915517039725619	1.0193474425084088
>10	3.937255537452088	25.697368338863523
>50	0.5455523202607602	10.630011402533333
>100	0.21710040985986603	11.065753951735779
>500	0.008403886833285138	1.5659181335820151
>1k	0.006302915124963853	3.929178281883152
>5k	0.0	0.0
>10k+	7.003239027737614E-4	5.262740904570138
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	25979	5.250658884020243	No Hit
CCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTGC	4432	0.8957588888709234	No Hit
CTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTGCT	3991	0.8066276456416641	No Hit
GCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTGC	3280	0.6629262534965318	No Hit
CGCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTG	1558	0.3148899704108526	No Hit
TCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTGC	1482	0.2995294840493476	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCC	1265	0.2556712532539978	No Hit
ACCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTG	1244	0.25142690833831877	No Hit
GCCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTG	1136	0.22959884877196954	No Hit
AGCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTG	1008	0.20372855595259268	No Hit
ACTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTGC	847	0.17118857826572026	No Hit
CGGCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCT	818	0.16532734004883018	No Hit
TCCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTG	792	0.16007243681989425	No Hit
TGCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTG	696	0.14066971720536162	No Hit
CGTTCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTC	693	0.14006338221740747	No Hit
AGAGCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTC	626	0.12652190081976492	No Hit
ATCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTG	605	0.12227755590408589	No Hit
ATTGCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTC	565	0.11419308939803062	No Hit
TGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTGCTT	533	0.1077255161931864	Illumina Single End Adapter 2 (95% over 21bp)
GGCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTG	525	0.10610862289197535	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	515	0.10408750626546154	No Hit
AGACCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTC	515	0.10408750626546154	No Hit
AGCTACCATTACATTTATTATTTTACTTCTACTCACAATTCTAGAATTTGC	498	0.10065160800038805	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.834114831762252	0.0
2	0.0	0.0	0.0	2.9257684285414007	0.0
3	0.0	0.0	0.0	4.842191213801801	0.0
4	0.0	0.0	0.0	6.071232234384853	0.0
5	0.0	0.0	0.0	12.41511310168642	0.0
6	0.0	0.0	0.0	13.826458841981017	0.0
7	0.0	0.0	0.0	19.152101152844924	0.0
8	0.0	0.0	0.0	25.497801025110352	0.0
9	0.0	0.0	0.0	30.198109851730884	0.0
10	0.0	0.0	0.0	32.724707746535806	0.0
11	0.0	0.0	0.0	35.17834333112358	0.0
12	0.0	0.0	0.0	36.9811793619739	0.0
13	0.0	0.0	0.0	37.64370139214513	0.0
14	0.0	0.0	0.0	38.11886591103853	0.0
15	0.0	0.0	0.0	39.842676281792166	0.0
16	0.0	0.0	0.0	41.72292107943797	0.0
17	0.0	0.0	0.0	44.376647210050606	0.0
18	0.0	0.0	0.0	45.34173039921096	0.0
19	0.0	0.0	0.0	46.498617556227465	0.0
20	0.0	0.0	0.0	47.539088395556774	0.0
21	2.0211166265138165E-4	0.0	0.0	48.441112745969896	0.0
22	0.0020211166265138164	0.0	0.0	49.4288324413472	0.0
23	0.0020211166265138164	0.0	0.0	50.13298947402461	0.0
24	0.004850679903633159	0.0	0.0	50.65666079195434	0.0
25	0.005254903228935922	0.0	0.0	51.18194900318528	0.0
26	0.005254903228935922	0.0	0.0	51.67813313499442	0.0
27	0.005659126554238686	0.0	0.0	52.2007938946109	0.0
28	0.006871796530146975	0.0	0.0	52.68909567157663	0.0
29	0.006871796530146975	0.0	0.0	53.33827833201287	0.0
30	0.006871796530146975	0.0	0.0	53.90883955567772	0.0
31	0.006871796530146975	0.0	0.0	54.308010089414196	0.0
32	0.006871796530146975	0.0	0.0	54.75144307727133	0.0
33	0.006871796530146975	0.0	0.0	55.192046501851344	0.0
34	0.006871796530146975	0.0	0.0	55.797775154817536	0.0
35	0.006871796530146975	0.0	0.0	56.216550519831195	0.0
36	0.006871796530146975	0.0	0.0	56.5969246689411	0.0
37	0.007073908192798357	0.0	0.0	56.98659595453296	0.0
38	0.007073908192798357	0.0	0.0	57.41062622277556	0.0
39	0.007073908192798357	0.0	0.0	57.81990233964461	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTGTTC	30	2.1629858E-6	45.000004	14
ACAACGA	30	2.1629858E-6	45.000004	33
GTTTGCG	25	3.887573E-5	45.000004	17
CGGGTAC	30	2.1629858E-6	45.000004	5
TCTGATT	30	2.1629858E-6	45.000004	25
CCTTAGG	30	2.1629858E-6	45.000004	1
CTGTCGG	25	3.887573E-5	45.000004	1
AGTCAGG	35	1.2100463E-7	45.000004	1
GTCGACT	25	3.887573E-5	45.000004	33
TCCGATT	30	2.1629858E-6	45.000004	41
CGGCCAT	25	3.887573E-5	45.000004	7
AACCATG	25	3.887573E-5	45.000004	10
CATGACA	25	3.887573E-5	45.000004	43
TGGGTAC	25	3.887573E-5	45.000004	5
GTCTCAC	70	0.0	45.000004	21
ACCTGGT	25	3.887573E-5	45.000004	10
TCGGGCA	25	3.887573E-5	45.000004	4
TATATGG	25	3.887573E-5	45.000004	1
ATGCTGG	30	2.1629858E-6	45.000004	1
TTGTAGC	35	1.2100463E-7	45.000004	1
>>END_MODULE