FastQCFastQC Report
Thu 26 May 2016
SRR1043368_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1043368_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1104179
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT917988.31368826974612No Hit
CCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTGCT96250.8716883766128499TruSeq Adapter, Index 13 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTGCTT85050.7702555473342637Illumina Single End Adapter 1 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTGCT55270.5005528994845945TruSeq Adapter, Index 13 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTGC42430.3842674059187867No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCG39680.35936202372984816No Hit
TCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTGCT31460.28491757224145725TruSeq Adapter, Index 13 (95% over 22bp)
CGTTCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCT26490.23990675424908461No Hit
ACCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTGC22460.20340904871402188No Hit
GCCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTGC21490.1946242411782872TruSeq Adapter, Index 13 (95% over 21bp)
TCCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTGC17220.15595297501582622No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCT17120.1550473247544103No Hit
ACTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTGCT16250.14716816748009154Illumina Single End Adapter 1 (95% over 21bp)
CGTTTCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTC15830.14336443638214455No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTT15690.14209652601616224No Hit
AGCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTGC14680.13294945837586117No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCC14670.13285889334971956No Hit
CGTCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTG14630.1324966332451532No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGT12620.11429306299069263No Hit
TGCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTGC12030.10894972644833853No Hit
ATCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTGC12030.10894972644833853No Hit
GGCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTGC11490.10405921503669241TruSeq Adapter, Index 13 (95% over 21bp)
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11230.10170452435701094No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAACGG351.0195254E-746.0000041
TATGCAA351.0195254E-746.0000049
CCGTACG351.0195254E-746.00000413
GCTAGTC351.0195254E-746.00000426
CCGTTGG700.046.0000041
AGTACGG700.046.0000041
CTTACGG351.0195254E-746.0000041
TGCAACG351.0195254E-746.00000411
ACAACGG453.092282E-1046.01
AGCGTCA206.311346E-446.010
ATCTCGG453.092282E-1046.01
CTCACGG405.6115823E-946.01
ACACGGC206.311346E-446.02
ACCGGCC301.8613373E-646.020
CAACGCA1050.046.016
CGGCATA206.311346E-446.09
GGCGTCA600.046.035
CCGTGTG206.311346E-446.035
CCTACGG253.4170684E-546.01
TTCGTTC206.311346E-446.040
Produced by FastQC (version 0.11.5)