##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043368_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1104179
Sequences flagged as poor quality	0
Sequence length	52
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.20146552325302	33.0	31.0	34.0	30.0	34.0
2	32.52692000119546	34.0	31.0	34.0	31.0	34.0
3	32.57760290677508	34.0	31.0	34.0	31.0	34.0
4	36.12812053118199	37.0	35.0	37.0	35.0	37.0
5	36.020310112762516	37.0	35.0	37.0	35.0	37.0
6	36.27437127494727	37.0	37.0	37.0	35.0	37.0
7	36.19293610909101	37.0	37.0	37.0	35.0	37.0
8	36.16523045629377	37.0	37.0	37.0	35.0	37.0
9	37.72081791086409	39.0	38.0	39.0	35.0	39.0
10	37.57844787846898	39.0	37.0	39.0	35.0	39.0
11	37.311214033231934	39.0	37.0	39.0	34.0	39.0
12	37.32270764069956	39.0	37.0	39.0	34.0	39.0
13	37.32259805701793	39.0	37.0	39.0	34.0	39.0
14	38.73911838569652	40.0	38.0	41.0	35.0	41.0
15	38.83627835704175	41.0	38.0	41.0	35.0	41.0
16	38.85857999472911	41.0	38.0	41.0	35.0	41.0
17	38.83276443402745	41.0	38.0	41.0	35.0	41.0
18	38.520787843275414	40.0	38.0	41.0	35.0	41.0
19	38.21125560257893	40.0	37.0	41.0	34.0	41.0
20	37.87164581105056	40.0	35.0	41.0	34.0	41.0
21	37.779062996126534	40.0	35.0	41.0	34.0	41.0
22	37.723341052492394	39.0	35.0	41.0	34.0	41.0
23	37.73105175881809	39.0	35.0	41.0	34.0	41.0
24	37.611987730250256	39.0	35.0	41.0	34.0	41.0
25	37.492229973582184	39.0	35.0	41.0	33.0	41.0
26	37.4298922547884	39.0	35.0	41.0	33.0	41.0
27	37.41100220163579	39.0	35.0	41.0	33.0	41.0
28	37.322755640163415	39.0	35.0	41.0	33.0	41.0
29	37.32580677589412	39.0	35.0	41.0	33.0	41.0
30	37.08640265754013	39.0	35.0	41.0	33.0	41.0
31	37.03606933296141	39.0	35.0	41.0	33.0	41.0
32	36.76155043702153	39.0	35.0	41.0	32.0	41.0
33	36.50824639845532	39.0	35.0	41.0	31.0	41.0
34	36.30675189439393	39.0	35.0	41.0	31.0	41.0
35	36.19451194054587	39.0	35.0	41.0	30.0	41.0
36	36.170037647881365	39.0	35.0	41.0	30.0	41.0
37	36.14784559387563	39.0	35.0	41.0	30.0	41.0
38	36.165052948842536	39.0	35.0	41.0	30.0	41.0
39	36.194016549852876	39.0	35.0	41.0	30.0	41.0
40	36.14445393364663	39.0	35.0	41.0	30.0	41.0
41	36.13474989109556	39.0	35.0	41.0	30.0	41.0
42	36.037428714003795	39.0	35.0	41.0	30.0	41.0
43	35.912421808420554	39.0	35.0	41.0	30.0	41.0
44	35.760629390705674	38.0	35.0	41.0	29.0	41.0
45	35.6704863975859	38.0	35.0	41.0	28.0	41.0
46	35.64592878509735	38.0	35.0	41.0	28.0	41.0
47	35.53378030192568	38.0	35.0	40.0	28.0	41.0
48	35.507395992859855	38.0	35.0	40.0	28.0	41.0
49	35.45560094875921	38.0	35.0	40.0	27.0	41.0
50	35.29986623545639	38.0	35.0	40.0	27.0	41.0
51	35.20721277981197	38.0	35.0	40.0	26.0	41.0
52	34.8390116095307	37.0	35.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	2.0
13	0.0
14	3.0
15	10.0
16	34.0
17	92.0
18	177.0
19	407.0
20	749.0
21	1218.0
22	1936.0
23	3421.0
24	6360.0
25	11549.0
26	15550.0
27	16802.0
28	16113.0
29	15751.0
30	17895.0
31	21348.0
32	26336.0
33	33903.0
34	57454.0
35	102362.0
36	130416.0
37	84974.0
38	141562.0
39	397292.0
40	463.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.09040653734585	19.092375421014165	25.41589724129874	30.401320800341246
2	34.74047233283734	19.339889637459144	26.374890303112085	19.54474772659143
3	20.202249816379407	21.13823936155279	40.37497543423666	18.284535387831138
4	21.86728782199263	23.51140530656714	37.74188786419593	16.879419007244294
5	19.249958566500542	28.283004838889347	33.49266740265844	18.974369191951666
6	69.14123525261756	1.657973933574176	24.629068294180563	4.571722519627705
7	69.83469165778374	1.4562856203568442	20.75252291521574	7.956499806643668
8	57.01838198335596	3.1465912682635695	29.48697629641571	10.348050451964763
9	30.90730760139434	17.221664241033384	32.687997145390376	19.1830310121819
10	23.89286519667554	18.948558159501314	44.703621423700326	12.454955220122823
11	20.475122240144035	15.673183424064396	43.10632605764101	20.745368278150554
12	16.45756711547675	19.083137788347724	45.84582753339812	18.613467562777412
13	11.604368494600966	22.41928165632565	45.36981775599789	20.60653209307549
14	10.494856359340288	22.471899936513918	45.908226836409675	21.125016867736118
15	11.033356004778211	18.0937148777508	49.96517774744856	20.907751370022435
16	11.448143824506715	25.13315322968468	41.70908883432849	21.709614111480114
17	13.139717382779423	22.525152171885175	43.049451221224096	21.28567922411131
18	14.712016801623651	28.8459570413855	40.8965394197861	15.545486737204747
19	14.366330096841182	24.127881439512976	37.998458583255065	23.50732988039077
20	11.954221190585947	30.275254283952147	36.505131867206316	21.265392658255593
21	12.83351702939469	24.633234285383075	38.27477247801308	24.258476207209156
22	10.935455211519146	27.118066907630013	33.698974532209	28.24750334864184
23	10.614311628821053	23.71300305475833	44.0288214139193	21.643863902501316
24	16.459468981025722	21.621132080939777	39.917803182273886	22.001595755760615
25	12.919010414072357	25.067946410862728	39.210852588212596	22.802190586852316
26	15.463344258494322	21.069862766815888	39.12146490741085	24.345328067278945
27	21.44425858488524	21.573313747137014	40.06017140336848	16.92225626460927
28	15.43545023044271	21.556468652274678	37.80519281746891	25.202888299813708
29	22.734176252219974	20.52194435865924	35.22191601180606	21.52196337731473
30	21.401964717677117	19.555796659780704	37.63973051470821	21.402508107833963
31	21.078919269430045	18.011029008883522	37.52262993590713	23.387421785779296
32	23.72758402396713	17.874185254383573	35.395529166919495	23.002701554729803
33	16.66441763518415	15.769182351774486	45.460020522034924	22.10637949100644
34	18.314602976510148	14.820513702941279	41.533211553561515	25.331671766987057
35	21.187959560904527	15.86092472325592	39.87025654354955	23.08085917229
36	17.850547782560618	16.46499344762036	39.03089988126925	26.65355888854977
37	14.937976541846929	17.252365784895385	41.35968896347422	26.449968709783466
38	14.047178944718203	17.597871359625568	34.22171586309828	34.13323383255795
39	20.275245227449535	15.771355912401885	35.652099885978636	28.301298974169946
40	14.746974901714305	14.460698854080725	41.557301850515174	29.23502439368979
41	17.9961763445963	18.991848241996994	34.31970722138349	28.692268192023214
42	19.276403554133886	15.010790822864772	40.41020522940574	25.302600393595604
43	22.952890790351926	18.131571058677988	38.38526180990583	20.530276341064265
44	16.72690750322185	20.83484652397845	38.91379930246816	23.524446670331532
45	18.34928938152238	18.10902036716873	38.55162976292793	24.990060488380962
46	22.77022113262433	15.942252116731073	36.089710092294816	25.19781665834978
47	16.071397844008988	14.908271213272487	40.663334477471494	28.35699646524703
48	17.465555856432697	13.06518236626489	35.91093473069131	33.558327046611105
49	20.056168429213017	13.306990986062948	41.30571220789383	25.331128376830208
50	17.827091440789943	12.959945805888356	42.22277366260362	26.99018909071808
51	15.132147957894507	12.866663828962514	39.94986320152801	32.05132501161497
52	13.717884509667364	13.120789292315829	49.10752694988766	24.053799248129152
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	391.0
1	2234.5
2	4078.0
3	51393.0
4	98708.0
5	53299.5
6	7891.0
7	7425.5
8	6960.0
9	7531.0
10	8102.0
11	8367.0
12	8632.0
13	8479.0
14	7749.5
15	7173.0
16	6445.0
17	5717.0
18	5183.5
19	4650.0
20	4333.5
21	4017.0
22	3798.5
23	3580.0
24	3511.0
25	3442.0
26	4037.0
27	4632.0
28	4712.5
29	4793.0
30	5252.5
31	5712.0
32	7208.0
33	8704.0
34	9891.5
35	11079.0
36	12750.0
37	14421.0
38	17235.5
39	21337.5
40	22625.0
41	28314.5
42	34004.0
43	40131.0
44	46258.0
45	58037.0
46	69816.0
47	87406.5
48	104997.0
49	135216.0
50	165435.0
51	168437.5
52	171440.0
53	143190.0
54	114940.0
55	87804.0
56	60668.0
57	45162.0
58	29656.0
59	22757.5
60	15859.0
61	12508.0
62	9157.0
63	7703.0
64	4920.0
65	3591.0
66	2970.5
67	2350.0
68	2071.0
69	1792.0
70	1608.0
71	1424.0
72	1332.0
73	1240.0
74	1018.0
75	796.0
76	598.0
77	400.0
78	325.5
79	251.0
80	164.0
81	77.0
82	63.5
83	50.0
84	37.0
85	24.0
86	15.0
87	6.0
88	6.5
89	6.0
90	5.0
91	3.5
92	2.0
93	2.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1104179.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.010376051107723
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.66856042673065	22.1082120154994
2	11.76576523763795	7.061900786211314
3	4.672500905179107	4.206705277906987
4	2.3873154242933214	2.8657693454260946
5	1.3683393027902637	2.0532188521123187
6	0.9093133454270754	1.6373301266734408
7	0.6316281464104772	1.3268778738769784
8	0.4728123186153044	1.1351420386593158
9	0.3535687510505329	0.9549658061052284
>10	3.160659561461801	20.374925047815513
>50	0.3733526852515308	7.5919474841727075
>100	0.21862150294842858	12.691369313458086
>500	0.00999239556410072	1.9577329750097319
>1k	0.0063587971771550044	3.542348070545366
>5k	9.083995967364292E-4	2.1497441974473275
>10k+	3.027998655788097E-4	8.341810789080178
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	91798	8.31368826974612	No Hit
CCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTGCT	9625	0.8716883766128499	TruSeq Adapter, Index 13 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTGCTT	8505	0.7702555473342637	Illumina Single End Adapter 1 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTGCT	5527	0.5005528994845945	TruSeq Adapter, Index 13 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTGC	4243	0.3842674059187867	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCG	3968	0.35936202372984816	No Hit
TCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTGCT	3146	0.28491757224145725	TruSeq Adapter, Index 13 (95% over 22bp)
CGTTCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCT	2649	0.23990675424908461	No Hit
ACCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTGC	2246	0.20340904871402188	No Hit
GCCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTGC	2149	0.1946242411782872	TruSeq Adapter, Index 13 (95% over 21bp)
TCCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTGC	1722	0.15595297501582622	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCT	1712	0.1550473247544103	No Hit
ACTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTGCT	1625	0.14716816748009154	Illumina Single End Adapter 1 (95% over 21bp)
CGTTTCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTC	1583	0.14336443638214455	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTT	1569	0.14209652601616224	No Hit
AGCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTGC	1468	0.13294945837586117	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCC	1467	0.13285889334971956	No Hit
CGTCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTG	1463	0.1324966332451532	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGT	1262	0.11429306299069263	No Hit
TGCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTGC	1203	0.10894972644833853	No Hit
ATCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTGC	1203	0.10894972644833853	No Hit
GGCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTGC	1149	0.10405921503669241	TruSeq Adapter, Index 13 (95% over 21bp)
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1123	0.10170452435701094	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.8032212168498043	0.0
2	0.0	0.0	0.0	2.676468217562551	0.0
3	0.0	0.0	0.0	4.4112412933047995	0.0
4	0.0	0.0	0.0	5.477463346069795	0.0
5	0.0	0.0	0.0	11.097204348208034	0.0
6	0.0	0.0	0.0	12.718499446194865	0.0
7	0.0	0.0	0.0	17.42932984597606	0.0
8	0.0	0.0	0.0	23.541835155350718	0.0
9	0.0	0.0	0.0	27.94130299525711	0.0
10	0.0	0.0	0.0	30.652548182858034	0.0
11	0.0	0.0	0.0	33.09354733245244	0.0
12	0.0	0.0	0.0	34.73440447608585	0.0
13	0.0	0.0	0.0	35.40431397445523	0.0
14	0.0	0.0	0.0	35.88883686431276	0.0
15	0.0	0.0	0.0	37.3451224846696	0.0
16	0.0	0.0	0.0	39.07844651999359	0.0
17	0.0	0.0	0.0	40.92823717893566	0.0
18	0.0	0.0	0.0	41.91358466335621	0.0
19	0.0	0.0	0.0	42.98225197182703	0.0
20	0.0	0.0	0.0	44.10091117472801	0.0
21	0.0	0.0	0.0	45.15083152278752	0.0
22	4.52825130707974E-4	0.0	0.0	46.202925431474426	0.0
23	4.52825130707974E-4	0.0	0.0	46.98287143660584	0.0
24	9.962152875575428E-4	0.0	0.0	47.649611159060264	0.0
25	9.962152875575428E-4	0.0	0.0	48.244532815784396	0.0
26	9.962152875575428E-4	0.0	0.0	48.86979375626597	0.0
27	9.962152875575428E-4	0.0	0.0	49.45085896399044	0.0
28	0.001267910365982327	0.0	0.0	50.03282982197633	0.0
29	0.0019018655489734908	0.0	0.0	50.7410483264036	0.0
30	0.0019018655489734908	0.0	0.0	51.348377391709136	0.0
31	0.0019018655489734908	0.0	0.0	51.87682431924534	0.0
32	0.0019018655489734908	0.0	0.0	52.40282599107572	0.0
33	0.0019018655489734908	0.0	0.0	52.90636753642299	0.0
34	0.0019018655489734908	0.0	0.0	53.504277839009795	0.0
35	0.0019018655489734908	0.0	0.0	53.958914270240605	0.0
36	0.0019018655489734908	0.0	0.0	54.43293161706571	0.0
37	0.0019018655489734908	0.0	0.0	54.91274512556388	0.0
38	0.0019018655489734908	0.0	0.0	55.3748078889383	0.0
39	0.0019018655489734908	0.0	0.0	55.849368625920256	0.0
40	0.0019018655489734908	0.0	0.0	56.30862387348428	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAAACGG	35	1.0195254E-7	46.000004	1
TATGCAA	35	1.0195254E-7	46.000004	9
CCGTACG	35	1.0195254E-7	46.000004	13
GCTAGTC	35	1.0195254E-7	46.000004	26
CCGTTGG	70	0.0	46.000004	1
AGTACGG	70	0.0	46.000004	1
CTTACGG	35	1.0195254E-7	46.000004	1
TGCAACG	35	1.0195254E-7	46.000004	11
ACAACGG	45	3.092282E-10	46.0	1
AGCGTCA	20	6.311346E-4	46.0	10
ATCTCGG	45	3.092282E-10	46.0	1
CTCACGG	40	5.6115823E-9	46.0	1
ACACGGC	20	6.311346E-4	46.0	2
ACCGGCC	30	1.8613373E-6	46.0	20
CAACGCA	105	0.0	46.0	16
CGGCATA	20	6.311346E-4	46.0	9
GGCGTCA	60	0.0	46.0	35
CCGTGTG	20	6.311346E-4	46.0	35
CCTACGG	25	3.4170684E-5	46.0	1
TTCGTTC	20	6.311346E-4	46.0	40
>>END_MODULE