SRR1043367_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1043367_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1066165
Sequences flagged as poor quality	0
Sequence length	51
%GC	43

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	83833	7.8630418368639	No Hit
CCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTGC	9266	0.8690962468285865	TruSeq Adapter, Index 13 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTGCT	8154	0.7647971936801526	Illumina Single End Adapter 1 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTGC	5296	0.49673362003067073	TruSeq Adapter, Index 13 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTG	4224	0.3961863313839791	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCC	3442	0.32283933537491855	No Hit
TCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTGC	2911	0.27303466161429046	TruSeq Adapter, Index 13 (95% over 21bp)
CGTTCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTC	2433	0.22820107581847088	No Hit
GCCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTG	2281	0.21394437071185043	No Hit
ACCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTG	2138	0.20053181261812195	No Hit
TCCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTG	1645	0.15429131513414904	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTC	1592	0.14932022716934057	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCT	1584	0.14856987426899215	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTT	1521	0.14266084517874814	No Hit
ACTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTGC	1518	0.14237946284111747	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGC	1451	0.13609525730069924	No Hit
AGCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTG	1446	0.13562628673798144	No Hit
CGTCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCT	1409	0.1321559045738699	No Hit
GGCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTG	1218	0.11424122907805077	No Hit
TGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTGCTT	1176	0.11030187635122143	TruSeq Adapter, Index 13 (95% over 23bp)
ATCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTG	1164	0.10917634700069877	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCG	1147	0.10758184708745831	No Hit
TGCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTG	1104	0.10354870024808543	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCCGGAT	20	7.033056E-4	45.000004	21
GGTACTA	20	7.033056E-4	45.000004	6
ACACGGT	20	7.033056E-4	45.000004	35
ACCGGCC	25	3.890614E-5	45.000004	20
TGAAGTA	20	7.033056E-4	45.000004	36
CAACGAC	25	3.890614E-5	45.000004	11
GACACGA	20	7.033056E-4	45.000004	24
TGGCGTC	20	7.033056E-4	45.000004	45
CGGCACG	20	7.033056E-4	45.000004	23
GCAGTAC	50	2.1827873E-11	45.000004	37
CGCGATA	20	7.033056E-4	45.000004	10
GCCGATC	20	7.033056E-4	45.000004	8
CTAACGG	80	0.0	45.000004	1
CTTGCGG	200	0.0	45.000004	1
ACGGATT	20	7.033056E-4	45.000004	6
AACGCCA	25	3.890614E-5	45.000004	14
GGAACCG	20	7.033056E-4	45.000004	7
CGCAGTA	50	2.1827873E-11	45.000004	36
GCGATCG	20	7.033056E-4	45.000004	8
CATCGAA	20	7.033056E-4	45.000004	13

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content