##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043367_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1066165
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.107447721506524	33.0	31.0	34.0	30.0	34.0
2	32.41743444963959	34.0	31.0	34.0	31.0	34.0
3	32.46086581345289	34.0	31.0	34.0	31.0	34.0
4	36.0698859932562	37.0	35.0	37.0	35.0	37.0
5	36.022821045522974	37.0	35.0	37.0	35.0	37.0
6	36.27430275801588	37.0	37.0	37.0	35.0	37.0
7	36.20305956395117	37.0	37.0	37.0	35.0	37.0
8	36.09691933237351	37.0	37.0	37.0	35.0	37.0
9	37.65452251762157	39.0	38.0	39.0	35.0	39.0
10	37.5051863454531	39.0	37.0	39.0	35.0	39.0
11	37.287204138196245	39.0	37.0	39.0	34.0	39.0
12	37.309113504945294	39.0	37.0	39.0	34.0	39.0
13	37.31347774500195	39.0	37.0	39.0	34.0	39.0
14	38.719724432897344	40.0	38.0	41.0	35.0	41.0
15	38.83432114166194	40.0	38.0	41.0	35.0	41.0
16	38.88555523769773	40.0	38.0	41.0	35.0	41.0
17	38.83887484582593	40.0	38.0	41.0	35.0	41.0
18	38.563978371077646	40.0	38.0	41.0	35.0	41.0
19	38.275070931797615	40.0	37.0	41.0	35.0	41.0
20	37.96352722139631	40.0	35.0	41.0	34.0	41.0
21	37.87379158010252	40.0	35.0	41.0	34.0	41.0
22	37.82903584342011	40.0	35.0	41.0	34.0	41.0
23	37.80297796307326	40.0	35.0	41.0	34.0	41.0
24	37.69462043867507	39.0	35.0	41.0	34.0	41.0
25	37.64921283291048	39.0	35.0	41.0	34.0	41.0
26	37.6286447219708	39.0	35.0	41.0	34.0	41.0
27	37.57809344707433	39.0	35.0	41.0	34.0	41.0
28	37.480048585350296	39.0	35.0	41.0	34.0	41.0
29	37.49654884562896	39.0	35.0	41.0	34.0	41.0
30	37.22760360732157	39.0	35.0	41.0	33.0	41.0
31	37.16200212912636	39.0	35.0	41.0	33.0	41.0
32	36.90283211322825	39.0	35.0	41.0	33.0	41.0
33	36.626874827067105	39.0	35.0	41.0	31.0	41.0
34	36.380655902229016	39.0	35.0	41.0	31.0	41.0
35	36.25994944497334	39.0	35.0	41.0	30.0	41.0
36	36.2159374956034	39.0	35.0	41.0	30.0	41.0
37	36.13777792367973	39.0	35.0	41.0	30.0	41.0
38	36.12732456983675	39.0	35.0	41.0	30.0	41.0
39	36.08250223933444	39.0	35.0	41.0	30.0	41.0
40	36.02118433825909	39.0	35.0	41.0	30.0	41.0
41	35.97076718894355	39.0	35.0	41.0	30.0	41.0
42	35.86855036509358	38.0	35.0	41.0	30.0	41.0
43	35.73170194106916	38.0	35.0	40.0	29.0	41.0
44	35.61574052796706	38.0	35.0	40.0	28.0	41.0
45	35.55219689260105	38.0	35.0	40.0	28.0	41.0
46	35.46782439866249	38.0	35.0	40.0	27.0	41.0
47	35.30745616297665	38.0	35.0	40.0	26.0	41.0
48	35.317251082149575	38.0	35.0	40.0	26.0	41.0
49	35.24614670337143	38.0	35.0	40.0	26.0	41.0
50	35.12662392781605	38.0	35.0	40.0	26.0	41.0
51	34.65918220913273	37.0	34.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	0.0
12	0.0
13	2.0
14	5.0
15	11.0
16	24.0
17	70.0
18	151.0
19	332.0
20	644.0
21	1043.0
22	1712.0
23	2735.0
24	5232.0
25	9600.0
26	14382.0
27	17000.0
28	16646.0
29	16172.0
30	17396.0
31	20538.0
32	25359.0
33	32871.0
34	56463.0
35	99931.0
36	119976.0
37	84495.0
38	147052.0
39	376276.0
40	46.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.52381667002762	19.335562506741454	25.331444945200786	29.809175878030135
2	34.350968189726736	19.407971561625075	26.506591381259	19.734468867389193
3	20.332124952516732	21.322028016301417	39.80912898097386	18.53671805020799
4	22.049307564964145	23.784686235245015	37.08562933504664	17.080376864744203
5	19.455712764909748	28.546331946743702	32.783949951461544	19.214005336885005
6	69.84866319941098	1.6093193830223278	24.01035487002481	4.531662547541891
7	70.54827348487336	1.4009088649505472	20.035172792203834	8.015644857972266
8	57.505826959241766	3.070350274113294	28.94158033700225	10.48224242964269
9	31.157747628181383	17.32152152809368	32.055826255785924	19.464904587939014
10	24.206478359353383	18.98233387890242	44.25074918047394	12.560438581270253
11	20.749790135673184	15.572167535043826	42.80144255345092	20.876599775832073
12	16.730712413181827	18.999873377948067	45.450469674018564	18.818944534851546
13	11.663016512453513	22.284543199223386	45.123222015354095	20.929218272969006
14	10.643568303217608	22.35723363644464	45.646217986896964	21.35298007344079
15	11.10606707216988	17.907265760928187	49.82512087716254	21.161546289739395
16	11.616588426744453	24.967054817969075	41.38721492451919	22.029141830767283
17	13.331613774603369	22.28444940511084	42.870943990845696	21.512992829440098
18	14.958566450783884	28.863262253028378	40.552353528769	15.62581776741874
19	14.578418912644853	24.098427541703206	37.66959147974281	23.653562065909124
20	12.024686610421465	30.28452444040088	36.103604976715616	21.58718397246205
21	12.929143237678971	24.577715456800778	37.83223047089334	24.66091083462691
22	11.042193281527718	27.04890893998584	33.2453231910633	28.663574587423145
23	10.765031678961511	23.747918943127942	43.63161424357393	21.855435134336616
24	16.62012915449297	21.46253159689166	39.59443425736167	22.3229049912537
25	12.93805367837061	25.029803079260716	39.046770434219845	22.98537280814883
26	15.609966562398878	20.774082810821966	38.84614482748918	24.76980579928998
27	21.688387819896544	21.498454741995847	39.73109227933762	17.082065158769986
28	15.691473646199228	21.443772774382953	37.447111844789504	25.417641734628315
29	22.992313572477055	20.414288595104885	34.78101419573893	21.812383636679126
30	21.7014252015401	19.416131649416364	37.13308915599367	21.749353993049855
31	21.399877129712568	17.789460355573482	37.05636557193305	23.7542969427809
32	24.05809607330948	17.579642925813545	35.05948891588075	23.302772084996228
33	16.87037184675918	15.467118128995045	45.26710218399591	22.395407840249867
34	18.671500189933077	14.464552860016976	41.32549839846553	25.53844855158442
35	21.417229040533126	15.511576538340687	39.836892038286756	23.234302382839427
36	18.182457687130977	16.06055347905812	38.953070115788826	26.803918718022068
37	15.184328879676222	16.92655452017277	41.223450404018145	26.66566619613287
38	14.156439200311397	17.240858591306225	33.85310904034554	34.74959316803684
39	20.55920049898468	15.372948840001314	35.22522311274521	28.842627548268794
40	14.828567810798516	14.028128854351813	41.3906853066833	29.75261802816637
41	18.194463333536554	18.767170184727505	33.94802868223962	29.090337799496325
42	19.466123911402082	14.616499322337537	40.30295498351568	25.614421782744696
43	23.295080967767653	17.803248090117382	38.057054958660245	20.844615983454716
44	16.963228018177297	20.519994559941473	38.83967303372367	23.677104388157556
45	18.481004347357118	17.778955414968603	38.50117008155398	25.238870156120303
46	23.15570291652793	15.630507473045919	35.84370149085742	25.370088119568734
47	16.39530466672607	14.554689002171333	40.45424488704844	28.59576144405416
48	17.783645120595782	12.693813809307189	35.52180009660793	34.000740973489094
49	20.20362701833206	12.943399942785591	41.01485229772127	25.838120741161074
50	18.10939207345955	12.55265366992914	42.03139288946833	27.306561367142983
51	15.384391721731628	12.521420230452135	39.593308728011145	32.5008793198051
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	381.0
1	2045.5
2	3710.0
3	46973.5
4	90237.0
5	49137.5
6	8038.0
7	7401.0
8	6764.0
9	7119.5
10	7475.0
11	7695.5
12	7916.0
13	7668.5
14	7421.0
15	6738.5
16	6056.0
17	5466.5
18	4877.0
19	4408.5
20	3940.0
21	3620.0
22	3300.0
23	3216.0
24	3132.0
25	3146.5
26	3676.5
27	4192.0
28	4295.0
29	4398.0
30	5620.0
31	6842.0
32	7886.5
33	8931.0
34	10253.5
35	11576.0
36	14088.0
37	16600.0
38	18368.5
39	20137.0
40	23942.0
41	27747.0
42	33165.0
43	38583.0
44	46068.5
45	53554.0
46	61109.0
47	68664.0
48	94670.5
49	120677.0
50	149705.0
51	178733.0
52	172611.5
53	166490.0
54	126862.5
55	87235.0
56	64899.5
57	42564.0
58	31608.0
59	20652.0
60	15752.5
61	10853.0
62	8948.0
63	7043.0
64	5868.5
65	4694.0
66	3734.5
67	2775.0
68	2382.0
69	1989.0
70	1766.0
71	1543.0
72	1456.5
73	1370.0
74	1163.5
75	696.5
76	436.0
77	367.0
78	298.0
79	212.0
80	126.0
81	89.5
82	53.0
83	36.5
84	20.0
85	13.5
86	7.0
87	9.5
88	12.0
89	7.0
90	2.0
91	2.0
92	2.0
93	1.0
94	0.0
95	1.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1066165.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.60832774506294
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.96021060467439	21.602298279320163
2	12.092102043505898	7.160538408617359
3	4.812543543838109	4.274740996000363
4	2.370671541594004	2.8076647991763557
5	1.4286457157686057	2.1149905292028452
6	0.9138452749072693	1.623445824463894
7	0.6475208097676055	1.3420405850143473
8	0.4895755157181188	1.159640986027222
9	0.3532315907907768	0.9412737039038946
>10	3.314773853957899	21.004356206279027
>50	0.3702555657586312	7.421210675341046
>100	0.22937304238090359	13.12745682201676
>500	0.009583831854350633	1.8936333055452332
>1k	0.006389221236233754	3.448550284059624
>5k	9.583831854350632E-4	2.148640068369906
>10k+	3.194610618116877E-4	7.929518526662016
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	83833	7.8630418368639	No Hit
CCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTGC	9266	0.8690962468285865	TruSeq Adapter, Index 13 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTGCT	8154	0.7647971936801526	Illumina Single End Adapter 1 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTGC	5296	0.49673362003067073	TruSeq Adapter, Index 13 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTG	4224	0.3961863313839791	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCC	3442	0.32283933537491855	No Hit
TCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTGC	2911	0.27303466161429046	TruSeq Adapter, Index 13 (95% over 21bp)
CGTTCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTC	2433	0.22820107581847088	No Hit
GCCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTG	2281	0.21394437071185043	No Hit
ACCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTG	2138	0.20053181261812195	No Hit
TCCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTG	1645	0.15429131513414904	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTC	1592	0.14932022716934057	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCT	1584	0.14856987426899215	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTT	1521	0.14266084517874814	No Hit
ACTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTGC	1518	0.14237946284111747	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGC	1451	0.13609525730069924	No Hit
AGCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTG	1446	0.13562628673798144	No Hit
CGTCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCT	1409	0.1321559045738699	No Hit
GGCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTG	1218	0.11424122907805077	No Hit
TGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTGCTT	1176	0.11030187635122143	TruSeq Adapter, Index 13 (95% over 23bp)
ATCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTG	1164	0.10917634700069877	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCG	1147	0.10758184708745831	No Hit
TGCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTG	1104	0.10354870024808543	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.7932168097808501	0.0
2	0.0	0.0	0.0	2.631675209747084	0.0
3	0.0	0.0	0.0	4.383749232060704	0.0
4	0.0	0.0	0.0	5.465101555575356	0.0
5	0.0	0.0	0.0	11.14114607026117	0.0
6	0.0	0.0	0.0	12.75871933518733	0.0
7	0.0	0.0	0.0	17.540812163220515	0.0
8	0.0	0.0	0.0	23.785155205807733	0.0
9	0.0	0.0	0.0	28.33885936979736	0.0
10	0.0	0.0	0.0	31.152307569653853	0.0
11	0.0	0.0	0.0	33.60389808331731	0.0
12	0.0	0.0	0.0	35.29613146182814	0.0
13	0.0	0.0	0.0	35.97885880703268	0.0
14	0.0	0.0	0.0	36.47653036818879	0.0
15	0.0	0.0	0.0	37.93606055347906	0.0
16	0.0	0.0	0.0	39.72368254444668	0.0
17	0.0	0.0	0.0	41.63642588154742	0.0
18	0.0	0.0	0.0	42.64067944455127	0.0
19	0.0	0.0	0.0	43.71837379767672	0.0
20	0.0	0.0	0.0	44.868570999798344	0.0
21	0.0	0.0	0.0	45.943826705997665	0.0
22	0.0	0.0	0.0	47.007639530466676	0.0
23	0.0	0.0	0.0	47.78650584102836	0.0
24	5.627646752613338E-4	0.0	0.0	48.464637274718264	0.0
25	5.627646752613338E-4	0.0	0.0	49.073361065125944	0.0
26	5.627646752613338E-4	0.0	0.0	49.7238232356155	0.0
27	5.627646752613338E-4	0.0	0.0	50.3089109096622	0.0
28	5.627646752613338E-4	0.0	0.0	50.91951058232075	0.0
29	9.379411254355564E-4	0.0	0.0	51.6356286315908	0.0
30	9.379411254355564E-4	0.0	0.0	52.23797442234551	0.0
31	9.379411254355564E-4	0.0	0.0	52.7685677170044	0.0
32	9.379411254355564E-4	0.0	0.0	53.302068629152146	0.0
33	9.379411254355564E-4	0.0	0.0	53.817467277578984	0.0
34	0.001031735237979112	0.0	0.0	54.4146543921438	0.0
35	0.001031735237979112	0.0	0.0	54.87996698447238	0.0
36	0.001031735237979112	0.0	0.0	55.35043825299086	0.0
37	0.001031735237979112	0.0	0.0	55.839480755792955	0.0
38	0.001031735237979112	0.0	0.0	56.31764314154	0.0
39	0.001031735237979112	0.0	0.0	56.820660967111095	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCCGGAT	20	7.033056E-4	45.000004	21
GGTACTA	20	7.033056E-4	45.000004	6
ACACGGT	20	7.033056E-4	45.000004	35
ACCGGCC	25	3.890614E-5	45.000004	20
TGAAGTA	20	7.033056E-4	45.000004	36
CAACGAC	25	3.890614E-5	45.000004	11
GACACGA	20	7.033056E-4	45.000004	24
TGGCGTC	20	7.033056E-4	45.000004	45
CGGCACG	20	7.033056E-4	45.000004	23
GCAGTAC	50	2.1827873E-11	45.000004	37
CGCGATA	20	7.033056E-4	45.000004	10
GCCGATC	20	7.033056E-4	45.000004	8
CTAACGG	80	0.0	45.000004	1
CTTGCGG	200	0.0	45.000004	1
ACGGATT	20	7.033056E-4	45.000004	6
AACGCCA	25	3.890614E-5	45.000004	14
GGAACCG	20	7.033056E-4	45.000004	7
CGCAGTA	50	2.1827873E-11	45.000004	36
GCGATCG	20	7.033056E-4	45.000004	8
CATCGAA	20	7.033056E-4	45.000004	13
>>END_MODULE