FastQCFastQC Report
Thu 26 May 2016
SRR1043366_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1043366_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences738910
Sequences flagged as poor quality0
Sequence length52
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT675259.138460705633975No Hit
CCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTGCT68700.929747871865315Illumina Single End Adapter 1 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTGCTT60400.8174202541581519Illumina Single End Adapter 1 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTGCT40860.5529766818692398Illumina Single End Adapter 1 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTGC28220.38191390020435506Illumina Single End Adapter 1 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTGCT22640.3063972608301417Illumina Single End Adapter 1 (95% over 22bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCG21080.28528508208036163No Hit
ACCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTGC18220.24657942103909813Illumina Single End Adapter 1 (95% over 21bp)
GCCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTGC15440.2089564358311567Illumina Single End Adapter 1 (95% over 21bp)
CGTTCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCT15270.20665574968534733No Hit
TCCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTGC12690.17173945406071103Illumina Single End Adapter 1 (95% over 21bp)
ACTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTGCT12230.16551406801910923Illumina Single End Adapter 1 (95% over 22bp)
AGCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTGC11360.15373996833173187Illumina Single End Adapter 1 (95% over 21bp)
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9520.1288384241653246No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCT9490.12843242072782884No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTT9190.12437238635287112No Hit
TGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTGCTTG9130.12356037947787958Illumina Single End Adapter 1 (95% over 24bp)
CGTCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTG9070.12274837260288804No Hit
GGCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTGC8850.11977101406125239Illumina Single End Adapter 1 (95% over 21bp)
TGCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTGC8430.11408696593631158Illumina Single End Adapter 1 (95% over 21bp)
CGTTTCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTC8390.11354562801965057No Hit
ATCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTGC8060.10907959020719708Illumina Single End Adapter 1 (95% over 21bp)
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8050.10894425572803183No Hit
GTGTTGGGTTAACAGAGAAGTTATAGGTGGATTATTTATAGTGTGATTATTG8010.1084029178113708No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCC7680.10393687999891732No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGAC501.6370905E-1146.024
CTTGTAT206.309984E-446.016
TGGAGTC206.309984E-446.09
TTAGGCG206.309984E-446.01
AACCGGA301.8604951E-646.014
CCCCTAG206.309984E-446.026
GCGACGC206.309984E-446.08
GTCTGCC206.309984E-446.041
TTTACGG551.8189894E-1246.01
TTAGCCG206.309984E-446.014
ATGTGCG405.6061253E-946.01
GGCGTAG206.309984E-446.07
GAATCAC206.309984E-446.08
TGAACGG453.092282E-1046.01
GCACGGC301.8604951E-646.02
TTCCGTA206.309984E-446.045
TAACGGG1200.046.02
TCGATGG600.046.01
ACGGGCC253.4159624E-546.04
GGCCCAC453.092282E-1046.07
Produced by FastQC (version 0.11.5)