##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043366_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	738910
Sequences flagged as poor quality	0
Sequence length	52
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.22731320458513	33.0	31.0	34.0	30.0	34.0
2	32.55004669039531	34.0	31.0	34.0	31.0	34.0
3	32.58727991230326	34.0	31.0	34.0	31.0	34.0
4	36.13414082905902	37.0	35.0	37.0	35.0	37.0
5	36.02422757846016	37.0	35.0	37.0	35.0	37.0
6	36.27149991203259	37.0	37.0	37.0	35.0	37.0
7	36.19939099484375	37.0	37.0	37.0	35.0	37.0
8	36.183256418237676	37.0	37.0	37.0	35.0	37.0
9	37.75362358067965	39.0	38.0	39.0	35.0	39.0
10	37.61003099159573	39.0	37.0	39.0	35.0	39.0
11	37.27241747980133	39.0	37.0	39.0	35.0	39.0
12	37.228912858128865	39.0	37.0	39.0	34.0	39.0
13	37.259099213706676	39.0	37.0	39.0	34.0	39.0
14	38.66925200633365	40.0	38.0	41.0	35.0	41.0
15	38.76760363237742	40.0	38.0	41.0	35.0	41.0
16	38.783884370221	41.0	38.0	41.0	35.0	41.0
17	38.777886346104395	41.0	38.0	41.0	35.0	41.0
18	38.48067558971999	40.0	38.0	41.0	35.0	41.0
19	38.160590599667074	40.0	37.0	41.0	35.0	41.0
20	37.82967208455698	40.0	35.0	41.0	34.0	41.0
21	37.75890568540147	39.0	35.0	41.0	34.0	41.0
22	37.721994559553934	39.0	35.0	41.0	34.0	41.0
23	37.71103382008634	39.0	35.0	41.0	34.0	41.0
24	37.6059019366364	39.0	35.0	41.0	34.0	41.0
25	37.503335994911424	39.0	35.0	41.0	34.0	41.0
26	37.48114249367311	39.0	35.0	41.0	34.0	41.0
27	37.48778741660013	39.0	35.0	41.0	34.0	41.0
28	37.45740076599315	39.0	35.0	41.0	34.0	41.0
29	37.41886833308522	39.0	35.0	41.0	34.0	41.0
30	37.33332476214965	39.0	35.0	41.0	33.0	41.0
31	37.2230217482508	39.0	35.0	41.0	33.0	41.0
32	37.04325425288601	39.0	35.0	41.0	33.0	41.0
33	36.939984571869374	39.0	35.0	41.0	33.0	41.0
34	36.74251667997456	39.0	35.0	41.0	32.0	41.0
35	36.645479151723485	39.0	35.0	41.0	32.0	41.0
36	36.609067410104075	39.0	35.0	41.0	32.0	41.0
37	36.585789879687646	39.0	35.0	41.0	32.0	41.0
38	36.53816026309023	39.0	35.0	41.0	32.0	41.0
39	36.50787646668742	39.0	35.0	41.0	32.0	41.0
40	36.46137418630144	39.0	35.0	41.0	32.0	41.0
41	36.476256918975245	39.0	35.0	41.0	32.0	41.0
42	36.39504269802818	39.0	35.0	41.0	32.0	41.0
43	36.27118864273051	39.0	35.0	41.0	31.0	41.0
44	36.116742228417536	38.0	35.0	41.0	31.0	41.0
45	36.06251776265039	38.0	35.0	41.0	31.0	41.0
46	36.059445669973336	38.0	35.0	41.0	31.0	41.0
47	35.97530010420755	38.0	35.0	41.0	31.0	41.0
48	35.97312663247215	38.0	35.0	41.0	31.0	41.0
49	35.91951929193001	38.0	35.0	40.0	31.0	41.0
50	35.764699354454535	38.0	35.0	40.0	30.0	41.0
51	35.711839060237374	38.0	35.0	40.0	30.0	41.0
52	35.401266730724984	37.0	35.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	1.0
14	1.0
15	9.0
16	9.0
17	56.0
18	91.0
19	233.0
20	426.0
21	704.0
22	1148.0
23	1891.0
24	3324.0
25	5968.0
26	8505.0
27	9475.0
28	8926.0
29	9069.0
30	10507.0
31	12674.0
32	16360.0
33	21461.0
34	39410.0
35	82430.0
36	90759.0
37	53675.0
38	86445.0
39	274353.0
40	999.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.8655316615014	19.243750930424543	24.723849995263294	30.16686741281076
2	34.67702426547211	19.561651621983735	25.814104559418606	19.94721955312555
3	20.530917161765302	21.284730210715786	39.31656088021545	18.86779174730346
4	21.58869145092095	23.55889079860876	37.62839858710804	17.22401916336225
5	19.1967898661542	28.666278707826393	33.01227483726029	19.124656588759116
6	69.1399493849048	1.4451015685266135	24.62830385297262	4.786645193595972
7	70.03694631281212	1.2988049965489707	20.473806011557564	8.19044267908135
8	57.328497381277835	2.8598882137202097	29.498179751255226	10.313434653746736
9	31.862067098834768	17.126578338363267	32.07224154497841	18.93911301782355
10	25.287788769944918	17.832753650647575	44.368190984017	12.511266595390508
11	21.985492143833486	15.703671624419755	41.31572180644463	20.995114425302134
12	15.114425302134224	21.925538969563277	45.12132736057165	17.838708367730845
13	10.55730738520253	25.054472127864013	44.73332340880486	19.654897078128595
14	9.221150072403947	22.698163511117727	47.46829789825554	20.612388518222787
15	9.190970483550094	18.135090877102762	51.89129934633446	20.782639293012682
16	9.389100161048031	25.108470585050952	41.21530362290401	24.287125630997007
17	11.888727991230326	23.12920382725907	42.73348581017986	22.248582371330745
18	14.272238838288832	29.41386637073527	40.63526004520172	15.67863474577418
19	14.71397057828423	23.829830425897605	37.097481425342735	24.35871757047543
20	11.552286476025497	31.28486554519495	35.52543611535911	21.637411863420443
21	12.47838031695335	25.057178817447323	37.0337388856559	25.430701979943432
22	10.396936027391698	28.042792762312054	32.54469421174432	29.01557699855192
23	9.836515949168371	23.79626747506462	44.92806972432366	21.43914685144334
24	16.139448647331882	21.46607841279723	40.32196072593415	22.072512213936747
25	10.870200701032601	27.069737857113857	38.40697784574576	23.65308359610778
26	13.261019609966032	23.124467120488287	36.69418467743027	26.92032859211541
27	19.738533786252724	23.55266541256716	37.89812020408439	18.810680597095722
28	14.772029069846123	23.38430932048558	34.29646371005941	27.547197899608882
29	16.42310971566226	23.519779134130005	31.090254564155313	28.96685658605243
30	19.88699570989701	28.13712089429024	28.077573723457526	23.898309672355225
31	23.048679812155743	23.414082905901935	27.603497042941633	25.93374023900069
32	16.21740130733107	26.07137540431176	27.341083487840194	30.370139800516977
33	16.76550594795036	30.572735515827365	28.382346970537686	24.27941156568459
34	17.493876114817773	28.71296910313841	29.70618884573223	24.086965936311593
35	23.71357810829465	25.651161846503634	27.952930668146326	22.682329377055392
36	16.209416573060317	35.842660134522475	27.779431865856463	20.168491426560745
37	18.812304610845707	31.525760918109107	33.07601737694713	16.58591709409806
38	18.368001515746165	30.82378097467892	29.271088495215924	21.53712901435899
39	22.964772435073282	27.343654842944336	32.21596676185192	17.475605960130462
40	14.731834729534043	24.172497327144036	38.44717218605784	22.64849575726408
41	19.63906294406626	25.435709355672543	30.57327685374403	24.351950846517166
42	20.04939708489532	20.086884735624093	36.460868035349364	23.40285014413122
43	22.850685469136973	22.740658537575616	34.49770608057815	19.91094991270926
44	14.470909853703429	26.813955691491522	34.85268841942862	23.862446035376433
45	18.73773531282565	23.860416018188953	34.431662854745504	22.970185814239894
46	23.422202974651853	20.943957992177666	33.58541635652515	22.04842267664533
47	17.149585199821356	19.112476485634243	38.44866086532866	25.289277449215735
48	17.53014575523406	17.527439065650753	33.77975666860646	31.162658510508724
49	19.628236185733037	17.410104072214477	39.265810450528484	23.695849291524002
50	17.583196871066843	17.062700464197263	40.16267204395664	25.191430620779254
51	15.16192770432123	15.717340406815444	38.95860118282335	30.16213070603998
52	14.687039016930342	15.532473508275704	47.56438537846287	22.216102096331085
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	333.0
1	1659.0
2	2985.0
3	36932.5
4	70880.0
5	37554.5
6	4229.0
7	3962.5
8	3696.0
9	4204.5
10	4713.0
11	5025.0
12	5337.0
13	5269.0
14	4723.5
15	4246.0
16	3751.5
17	3257.0
18	2966.5
19	2676.0
20	2492.5
21	2309.0
22	2138.0
23	1967.0
24	1965.0
25	1963.0
26	2262.0
27	2561.0
28	2853.5
29	3146.0
30	3731.5
31	4317.0
32	5257.0
33	6197.0
34	7293.0
35	8389.0
36	9801.0
37	11213.0
38	11684.5
39	13937.0
40	15718.0
41	21341.5
42	26965.0
43	35829.0
44	44693.0
45	59727.0
46	74761.0
47	93729.0
48	112697.0
49	115007.0
50	117317.0
51	97853.0
52	78389.0
53	60568.0
54	42747.0
55	32184.5
56	21622.0
57	17698.0
58	13774.0
59	11865.5
60	9957.0
61	7967.0
62	5977.0
63	4976.5
64	3341.5
65	2707.0
66	2160.5
67	1614.0
68	1389.5
69	1165.0
70	994.0
71	823.0
72	935.0
73	1047.0
74	837.5
75	628.0
76	437.0
77	246.0
78	196.0
79	146.0
80	108.5
81	71.0
82	49.5
83	28.0
84	34.0
85	40.0
86	24.0
87	8.0
88	13.0
89	11.0
90	4.0
91	2.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	738910.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.228861608193
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.99821541972803	21.171142439449486
2	11.166425856418954	6.304309803179973
3	4.212053832586222	3.5670445417900627
4	2.0081508011265763	2.2675124421353616
5	1.204890270373851	1.7006340347720847
6	0.7885421061646093	1.3355787592292274
7	0.552425508241547	1.0916040254690418
8	0.44444973566504614	1.003704806390924
9	0.3380743706458532	0.8589109160014864
>10	3.4899112162402415	22.606107325730143
>50	0.5540034622792284	10.57810520102436
>100	0.22314028341752115	11.237327732237397
>500	0.013465361930367655	2.6705571734729943
>1k	0.004809057832274163	2.6880694688866797
>5k	9.618115664548325E-4	1.752587083648656
>10k+	4.8090578322741625E-4	9.166804246582146
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	67525	9.138460705633975	No Hit
CCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTGCT	6870	0.929747871865315	Illumina Single End Adapter 1 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTGCTT	6040	0.8174202541581519	Illumina Single End Adapter 1 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTGCT	4086	0.5529766818692398	Illumina Single End Adapter 1 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTGC	2822	0.38191390020435506	Illumina Single End Adapter 1 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTGCT	2264	0.3063972608301417	Illumina Single End Adapter 1 (95% over 22bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCG	2108	0.28528508208036163	No Hit
ACCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTGC	1822	0.24657942103909813	Illumina Single End Adapter 1 (95% over 21bp)
GCCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTGC	1544	0.2089564358311567	Illumina Single End Adapter 1 (95% over 21bp)
CGTTCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCT	1527	0.20665574968534733	No Hit
TCCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTGC	1269	0.17173945406071103	Illumina Single End Adapter 1 (95% over 21bp)
ACTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTGCT	1223	0.16551406801910923	Illumina Single End Adapter 1 (95% over 22bp)
AGCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTGC	1136	0.15373996833173187	Illumina Single End Adapter 1 (95% over 21bp)
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	952	0.1288384241653246	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCT	949	0.12843242072782884	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTT	919	0.12437238635287112	No Hit
TGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTGCTTG	913	0.12356037947787958	Illumina Single End Adapter 1 (95% over 24bp)
CGTCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTG	907	0.12274837260288804	No Hit
GGCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTGC	885	0.11977101406125239	Illumina Single End Adapter 1 (95% over 21bp)
TGCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTGC	843	0.11408696593631158	Illumina Single End Adapter 1 (95% over 21bp)
CGTTTCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTC	839	0.11354562801965057	No Hit
ATCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTGC	806	0.10907959020719708	Illumina Single End Adapter 1 (95% over 21bp)
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	805	0.10894425572803183	No Hit
GTGTTGGGTTAACAGAGAAGTTATAGGTGGATTATTTATAGTGTGATTATTG	801	0.1084029178113708	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCC	768	0.10393687999891732	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.839073770824593	0.0
2	0.0	0.0	0.0	2.8440540796578744	0.0
3	0.0	0.0	0.0	4.710587216305098	0.0
4	0.0	0.0	0.0	5.8759524163971255	0.0
5	0.0	0.0	0.0	11.791422500710507	0.0
6	0.0	0.0	0.0	13.512877075692574	0.0
7	0.0	0.0	0.0	18.352302716162995	0.0
8	0.0	0.0	0.0	24.419618086099796	0.0
9	0.0	0.0	0.0	28.937083000636072	0.0
10	0.0	0.0	0.0	31.581112720087695	0.0
11	0.0	0.0	0.0	33.870972107563844	0.0
12	0.0	0.0	0.0	35.40742444952701	0.0
13	0.0	0.0	0.0	35.99910679243751	0.0
14	0.0	0.0	0.0	36.438943849724595	0.0
15	0.0	0.0	0.0	37.78985262075219	0.0
16	0.0	0.0	0.0	39.3214329214654	0.0
17	0.0	0.0	0.0	41.04045147582249	0.0
18	0.0	0.0	0.0	41.84230826487664	0.0
19	0.0	0.0	0.0	42.74295922372143	0.0
20	0.0	0.0	0.0	43.668917730170115	0.0
21	1.3533447916525693E-4	0.0	0.0	44.52287829370289	0.0
22	6.766723958262846E-4	0.0	0.0	45.397545032547946	0.0
23	6.766723958262846E-4	0.0	0.0	46.04985722212448	0.0
24	0.0012180103124873123	0.0	0.0	46.54233939180685	0.0
25	0.0012180103124873123	0.0	0.0	46.995709897010464	0.0
26	0.0012180103124873123	0.0	0.0	47.481831346172065	0.0
27	0.0012180103124873123	0.0	0.0	47.93411917554235	0.0
28	0.0013533447916525693	0.0	0.0	48.40467715959995	0.0
29	0.0013533447916525693	0.0	0.0	48.9922994681355	0.0
30	0.0013533447916525693	0.0	0.0	49.47016551406802	0.0
31	0.0013533447916525693	0.0	0.0	49.86791354833471	0.0
32	0.0013533447916525693	0.0	0.0	50.26106021030978	0.0
33	0.0013533447916525693	0.0	0.0	50.63945541405584	0.0
34	0.0013533447916525693	0.0	0.0	51.118674804780014	0.0
35	0.0013533447916525693	0.0	0.0	51.46607841279723	0.0
36	0.0013533447916525693	0.0	0.0	51.81185800706446	0.0
37	0.0013533447916525693	0.0	0.0	52.17563708706067	0.0
38	0.0013533447916525693	0.0	0.0	52.52926608111949	0.0
39	0.0013533447916525693	0.0	0.0	52.873150992678404	0.0
40	0.0013533447916525693	0.0	0.0	53.23314070725799	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGAC	50	1.6370905E-11	46.0	24
CTTGTAT	20	6.309984E-4	46.0	16
TGGAGTC	20	6.309984E-4	46.0	9
TTAGGCG	20	6.309984E-4	46.0	1
AACCGGA	30	1.8604951E-6	46.0	14
CCCCTAG	20	6.309984E-4	46.0	26
GCGACGC	20	6.309984E-4	46.0	8
GTCTGCC	20	6.309984E-4	46.0	41
TTTACGG	55	1.8189894E-12	46.0	1
TTAGCCG	20	6.309984E-4	46.0	14
ATGTGCG	40	5.6061253E-9	46.0	1
GGCGTAG	20	6.309984E-4	46.0	7
GAATCAC	20	6.309984E-4	46.0	8
TGAACGG	45	3.092282E-10	46.0	1
GCACGGC	30	1.8604951E-6	46.0	2
TTCCGTA	20	6.309984E-4	46.0	45
TAACGGG	120	0.0	46.0	2
TCGATGG	60	0.0	46.0	1
ACGGGCC	25	3.4159624E-5	46.0	4
GGCCCAC	45	3.092282E-10	46.0	7
>>END_MODULE