FastQCFastQC Report
Thu 26 May 2016
SRR1043365_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1043365_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences720406
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT606608.42025191350433No Hit
CCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTGC63170.8768666557468983Illumina Single End Adapter 1 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTGCT60200.8356399030546665Illumina Single End Adapter 1 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTGC41530.5764804846156195Illumina Single End Adapter 1 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTG29890.41490492860970063No Hit
TCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTGC22250.3088536186539257Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCC20340.282340791164982No Hit
ACCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTG17960.24930386476514632No Hit
GCCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTG15530.21557288528968385No Hit
CGTTCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTC15150.2102980819149202No Hit
ACTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTGC12320.17101467783444335Illumina Single End Adapter 1 (95% over 21bp)
TCCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTG12020.16685035938068254No Hit
AGCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTG11990.16643392753530648No Hit
TGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTGCTT10780.14963784310513792Illumina Single End Adapter 1 (95% over 23bp)
CGTCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCT9530.13228651621446796No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTT9230.12812219776070716No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTC9130.12673409160945356No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCT9060.12576241730357604No Hit
GGCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTG8730.12118166700443916No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8660.12020999269856164No Hit
TGCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTG8620.1196547502380602No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7780.10799465856752997No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGC7760.10771703733727926No Hit
ATCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTG7730.10730060549190318No Hit
GTGTTGGGTTAACAGAGAAGTTATAGGTGGATTATTTATAGTGTGATTATT7690.10674536303140175No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAACCG253.88935E-545.00000418
AAGTAGG1200.045.0000041
CGGGTAG600.045.0000045
GGTACGT253.88935E-545.0000047
TCCGCGG302.1643682E-645.0000041
GGCACAT253.88935E-545.0000047
CTGAGAT502.1827873E-1145.00000410
CGCATAC253.88935E-545.00000426
TTGTCGG253.88935E-545.0000041
GAATCTA302.1643682E-645.0000048
GAATCAC253.88935E-545.0000048
TCGGCAG302.1643682E-645.00000418
GTTACCT253.88935E-545.0000049
CCCCATG253.88935E-545.00000412
CTCGCGG502.1827873E-1145.0000041
TCGTACC253.88935E-545.00000429
TCATCGG253.88935E-545.0000041
TTCGGCA302.1643682E-645.00000417
GCGGATA253.88935E-545.0000048
CGGTCTA1000.045.00000430
Produced by FastQC (version 0.11.5)