##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043365_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	720406
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.14844268370891	33.0	31.0	34.0	30.0	34.0
2	32.444255045071806	34.0	31.0	34.0	31.0	34.0
3	32.48069699586067	34.0	31.0	34.0	31.0	34.0
4	36.07706348919915	37.0	35.0	37.0	35.0	37.0
5	36.03388505925825	37.0	35.0	37.0	35.0	37.0
6	36.26875817247497	37.0	37.0	37.0	35.0	37.0
7	36.20700132980569	37.0	37.0	37.0	35.0	37.0
8	36.11893987557016	37.0	37.0	37.0	35.0	37.0
9	37.69546477958262	39.0	38.0	39.0	35.0	39.0
10	37.543611519060086	39.0	37.0	39.0	35.0	39.0
11	37.257504795906755	39.0	37.0	39.0	35.0	39.0
12	37.21180278898288	39.0	37.0	39.0	34.0	39.0
13	37.25595566944195	39.0	37.0	39.0	34.0	39.0
14	38.65676299197952	40.0	38.0	41.0	35.0	41.0
15	38.77370815901033	40.0	38.0	41.0	35.0	41.0
16	38.80926033375624	40.0	38.0	41.0	35.0	41.0
17	38.791135831739325	40.0	38.0	41.0	35.0	41.0
18	38.53040091281861	40.0	38.0	41.0	35.0	41.0
19	38.24546436315078	40.0	37.0	41.0	35.0	41.0
20	37.94815007093222	40.0	35.0	41.0	34.0	41.0
21	37.880690888193605	40.0	35.0	41.0	34.0	41.0
22	37.85495817636166	40.0	35.0	41.0	34.0	41.0
23	37.8076556830454	40.0	35.0	41.0	34.0	41.0
24	37.71640019655583	39.0	35.0	41.0	34.0	41.0
25	37.68717500964734	39.0	35.0	41.0	34.0	41.0
26	37.67604933884504	39.0	35.0	41.0	34.0	41.0
27	37.66470712348315	39.0	35.0	41.0	34.0	41.0
28	37.59844865256536	39.0	35.0	41.0	34.0	41.0
29	37.578343323070605	39.0	35.0	41.0	34.0	41.0
30	37.44395937846159	39.0	35.0	41.0	34.0	41.0
31	37.32005147097609	39.0	35.0	41.0	33.0	41.0
32	37.13840112381074	39.0	35.0	41.0	33.0	41.0
33	36.99165609392482	39.0	35.0	41.0	33.0	41.0
34	36.75725354869338	39.0	35.0	41.0	32.0	41.0
35	36.65419360749355	39.0	35.0	41.0	32.0	41.0
36	36.60274761731579	39.0	35.0	41.0	32.0	41.0
37	36.538973856408745	39.0	35.0	41.0	32.0	41.0
38	36.48789849057337	39.0	35.0	41.0	31.0	41.0
39	36.41454263290422	39.0	35.0	41.0	31.0	41.0
40	36.343120407103775	39.0	35.0	41.0	31.0	41.0
41	36.32414499601614	39.0	35.0	41.0	31.0	41.0
42	36.22787289389594	38.0	35.0	41.0	31.0	41.0
43	36.09607499104671	38.0	35.0	40.0	31.0	41.0
44	36.00082731126615	38.0	35.0	40.0	30.0	41.0
45	35.975909417744994	38.0	35.0	40.0	30.0	41.0
46	35.91025338489685	38.0	35.0	40.0	30.0	41.0
47	35.78027112489346	38.0	35.0	40.0	30.0	41.0
48	35.79494340691221	38.0	35.0	40.0	30.0	41.0
49	35.72676518518724	38.0	35.0	40.0	30.0	41.0
50	35.61335552452367	38.0	35.0	40.0	29.0	41.0
51	35.207859179407166	37.0	35.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	0.0
13	2.0
14	3.0
15	2.0
16	14.0
17	31.0
18	88.0
19	182.0
20	346.0
21	644.0
22	1009.0
23	1616.0
24	2807.0
25	5131.0
26	7872.0
27	9216.0
28	9475.0
29	9238.0
30	10558.0
31	12651.0
32	15701.0
33	21125.0
34	39350.0
35	81081.0
36	82706.0
37	53639.0
38	91698.0
39	264111.0
40	109.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.227988106706494	19.273298667695716	24.996460329314303	29.502252896283487
2	34.08841680941025	19.64170204023842	26.097506128488657	20.17237502186267
3	20.748994317093416	21.491214676168717	38.61475334741799	19.145037659319883
4	21.83088425138047	23.728008928298767	36.990807961066395	17.450298859254364
5	19.481931022229134	28.77974364455599	32.3327401493047	19.405585183910183
6	69.85519276630122	1.4004602959997556	23.95079996557497	4.793546972124052
7	70.72511889129186	1.2749754999264304	19.648781381609815	8.351124227171901
8	57.789635288989814	2.762747672840037	28.90175817525118	10.545858862918966
9	32.15478494071399	17.205575744788355	31.350238615447402	19.28940069905026
10	25.659975069613523	17.839246202835625	43.907602102147955	12.593176625402897
11	22.26550028733797	15.596483094255184	40.91345713389394	21.224559484512902
12	15.309839174021317	21.90542555170279	44.67453075071557	18.110204523560324
13	10.735335352565082	24.973695388433743	44.32694897044166	19.964020288559507
14	9.337373647637582	22.69775654283834	47.10982418247488	20.855045627049194
15	9.304891963698248	18.021088108649845	51.54357404019401	21.130445887457906
16	9.425934820087562	25.104732609112084	40.89749391315453	24.571838657645827
17	12.045846369963604	23.02215695038631	42.3445945758364	22.587402103813684
18	14.433666571350045	29.55916524848488	40.233007498549426	15.774160681615646
19	14.863285425163033	23.846553193615822	36.61615811084305	24.67400327037809
20	11.634411706732037	31.479054866283736	34.93044200076068	21.956091426223544
21	12.49767492219665	25.087658903451665	36.41155126414827	26.00311491020341
22	10.612210336948886	28.03349777764205	31.87105604339775	29.48323584201131
23	9.974097939217607	23.76826400668512	44.48519307168458	21.772444982412694
24	16.267771229001422	21.396407026038094	39.83101195714639	22.504809787814093
25	10.923701357290195	27.067514706984674	38.01814532360919	23.990638612115948
26	13.436312301674333	22.948726134984994	36.24386804107684	27.371093522263834
27	20.10755046459913	23.42359724932885	37.46845528771276	19.00039699835926
28	14.925611391354321	23.500914761953677	33.56121409316413	28.012259753527875
29	16.69516911297241	23.393058914001273	30.445193404830057	29.466578568196265
30	20.269126020605047	28.06070465820662	27.32126051143383	24.348908809754498
31	23.52617829390649	23.34877832777628	26.785312726434817	26.33973065188241
32	16.33065243765321	26.02116029016971	26.575014644519896	31.07317262765718
33	16.96612743369711	30.65799007781723	27.672590178316113	24.703292310169545
34	17.897546661188272	28.72255367112434	28.927854570894745	24.45204509679264
35	24.189415412975464	25.496594975610975	27.307101828691042	23.006887782722522
36	16.27832083575095	36.07049358278526	27.08250625341821	20.568679328045576
37	19.15503202360891	31.704344494632192	32.461139968295655	16.679483513463243
38	18.785101734299825	30.79124826833758	28.56430956988143	21.85934042748117
39	23.34072731209901	27.355269112139542	31.506539368078556	17.79746420768289
40	14.845795287657237	24.080171458871803	38.08158177472148	22.99245147874948
41	20.069655166669907	25.408311424391243	29.92451478749483	24.59751862144402
42	20.450551494573894	19.827708264506402	36.04300908099044	23.67873115992926
43	23.21926802386432	22.639733705715944	33.97806237038559	20.16293590003415
44	14.716284983745275	26.683009303087424	34.51442658722998	24.086279125937317
45	19.052312168416144	23.60099721545906	34.05496345116504	23.29172716495976
46	23.866264300963625	20.706934700710434	33.027348467391995	22.399452530933946
47	17.47917146720044	18.842430518346596	38.040355022029246	25.638042992423713
48	17.86353806048256	17.284003742334182	33.19031212954917	31.662146067634083
49	19.97193249362165	17.056631954758846	38.856145007120986	24.115290544498517
50	17.883526789060614	16.737506350585644	39.92290458435937	25.456062275994366
51	15.44642881930467	15.479604556319632	38.42569328961724	30.648273334758457
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	278.0
1	1528.5
2	2779.0
3	33330.0
4	63881.0
5	34217.5
6	4554.0
7	4119.5
8	3685.0
9	4023.0
10	4361.0
11	4526.5
12	4692.0
13	4570.5
14	4449.0
15	4062.5
16	3676.0
17	3205.5
18	2735.0
19	2462.0
20	2189.0
21	2049.5
22	1910.0
23	1826.0
24	1742.0
25	1752.0
26	2177.0
27	2592.0
28	2888.5
29	3185.0
30	4180.5
31	5176.0
32	5685.0
33	6194.0
34	7724.5
35	9255.0
36	10345.5
37	11436.0
38	12313.0
39	13190.0
40	17087.5
41	20985.0
42	27199.0
43	33413.0
44	40867.0
45	48321.0
46	65291.0
47	82261.0
48	103078.5
49	123896.0
50	119333.0
51	114770.0
52	88555.0
53	62340.0
54	46358.0
55	30376.0
56	23662.5
57	16949.0
58	14439.5
59	11930.0
60	9537.0
61	7144.0
62	5894.0
63	4644.0
64	3925.5
65	3207.0
66	2470.0
67	1733.0
68	1514.5
69	1296.0
70	1148.0
71	1000.0
72	985.5
73	971.0
74	845.5
75	561.5
76	403.0
77	272.0
78	141.0
79	104.0
80	67.0
81	60.0
82	53.0
83	44.5
84	36.0
85	21.0
86	6.0
87	10.5
88	15.0
89	10.0
90	5.0
91	3.0
92	1.0
93	0.5
94	0.0
95	0.5
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	720406.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.834424121254514
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.26375362580704	20.670888152570658
2	11.449468119594986	6.373787032071784
3	4.288671595272254	3.5811811229875534
4	2.122303735837464	2.362924091896915
5	1.2248717904049493	1.7046800454145865
6	0.8228777719102074	1.374259734198099
7	0.5919536445244421	1.1533682161270957
8	0.42591881302529816	0.948416390637396
9	0.3484727118795794	0.8729583531425946
>10	3.643603194530759	23.179845169721105
>50	0.5685477532908196	10.67094589143255
>100	0.22892852013029125	11.353110013393335
>500	0.013584769326412888	2.5930919487336523
>1k	0.0055345356515015464	2.9376051369970346
>5k	0.0010062792093639176	1.7277476437420107
>10k+	5.031396046819588E-4	8.495191056933644
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	60660	8.42025191350433	No Hit
CCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTGC	6317	0.8768666557468983	Illumina Single End Adapter 1 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTGCT	6020	0.8356399030546665	Illumina Single End Adapter 1 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTGC	4153	0.5764804846156195	Illumina Single End Adapter 1 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTG	2989	0.41490492860970063	No Hit
TCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTGC	2225	0.3088536186539257	Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCC	2034	0.282340791164982	No Hit
ACCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTG	1796	0.24930386476514632	No Hit
GCCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTG	1553	0.21557288528968385	No Hit
CGTTCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTC	1515	0.2102980819149202	No Hit
ACTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTGC	1232	0.17101467783444335	Illumina Single End Adapter 1 (95% over 21bp)
TCCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTG	1202	0.16685035938068254	No Hit
AGCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTG	1199	0.16643392753530648	No Hit
TGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTGCTT	1078	0.14963784310513792	Illumina Single End Adapter 1 (95% over 23bp)
CGTCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCT	953	0.13228651621446796	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTT	923	0.12812219776070716	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTC	913	0.12673409160945356	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCT	906	0.12576241730357604	No Hit
GGCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTG	873	0.12118166700443916	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	866	0.12020999269856164	No Hit
TGCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTG	862	0.1196547502380602	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	778	0.10799465856752997	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGC	776	0.10771703733727926	No Hit
ATCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTG	773	0.10730060549190318	No Hit
GTGTTGGGTTAACAGAGAAGTTATAGGTGGATTATTTATAGTGTGATTATT	769	0.10674536303140175	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.8577107908595987	0.0
2	0.0	0.0	0.0	2.8568612698950315	0.0
3	0.0	0.0	0.0	4.78466309275603	0.0
4	0.0	0.0	0.0	5.972326715768609	0.0
5	0.0	0.0	0.0	12.009200367570509	0.0
6	0.0	0.0	0.0	13.734893934808984	0.0
7	0.0	0.0	0.0	18.696262940619594	0.0
8	0.0	0.0	0.0	24.94218537880029	0.0
9	0.0	0.0	0.0	29.628570556047563	0.0
10	0.0	0.0	0.0	32.346898832047486	0.0
11	0.0	0.0	0.0	34.70168210703409	0.0
12	0.0	0.0	0.0	36.30036396143286	0.0
13	0.0	0.0	0.0	36.923207191500346	0.0
14	0.0	0.0	0.0	37.35948895483936	0.0
15	0.0	0.0	0.0	38.75925519776348	0.0
16	0.0	0.0	0.0	40.364461151073144	0.0
17	0.0	0.0	0.0	42.155118086190285	0.0
18	0.0	0.0	0.0	42.982984594797934	0.0
19	0.0	0.0	0.0	43.90996188260509	0.0
20	0.0	0.0	0.0	44.84179754194163	0.0
21	0.0	0.0	0.0	45.704644325560864	0.0
22	6.940530756267993E-4	0.0	0.0	46.62995588598651	0.0
23	8.328636907521592E-4	0.0	0.0	47.30277093749913	0.0
24	0.0013881061512535987	0.0	0.0	47.81748069838397	0.0
25	0.0013881061512535987	0.0	0.0	48.28610533504718	0.0
26	0.0013881061512535987	0.0	0.0	48.78110398858421	0.0
27	0.0013881061512535987	0.0	0.0	49.23432064696851	0.0
28	0.0015269167663789585	0.0	0.0	49.72237876974928	0.0
29	0.0015269167663789585	0.0	0.0	50.32537208185384	0.0
30	0.0015269167663789585	0.0	0.0	50.80843302249009	0.0
31	0.0015269167663789585	0.0	0.0	51.20140587390999	0.0
32	0.0015269167663789585	0.0	0.0	51.60798216561217	0.0
33	0.0015269167663789585	0.0	0.0	52.00373122933457	0.0
34	0.0015269167663789585	0.0	0.0	52.489984814118706	0.0
35	0.0015269167663789585	0.0	0.0	52.8556119743589	0.0
36	0.0015269167663789585	0.0	0.0	53.20846855800757	0.0
37	0.0015269167663789585	0.0	0.0	53.57034783163938	0.0
38	0.0016657273815043185	0.0	0.0	53.91806842252841	0.0
39	0.0016657273815043185	0.0	0.0	54.28522249953498	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACAACCG	25	3.88935E-5	45.000004	18
AAGTAGG	120	0.0	45.000004	1
CGGGTAG	60	0.0	45.000004	5
GGTACGT	25	3.88935E-5	45.000004	7
TCCGCGG	30	2.1643682E-6	45.000004	1
GGCACAT	25	3.88935E-5	45.000004	7
CTGAGAT	50	2.1827873E-11	45.000004	10
CGCATAC	25	3.88935E-5	45.000004	26
TTGTCGG	25	3.88935E-5	45.000004	1
GAATCTA	30	2.1643682E-6	45.000004	8
GAATCAC	25	3.88935E-5	45.000004	8
TCGGCAG	30	2.1643682E-6	45.000004	18
GTTACCT	25	3.88935E-5	45.000004	9
CCCCATG	25	3.88935E-5	45.000004	12
CTCGCGG	50	2.1827873E-11	45.000004	1
TCGTACC	25	3.88935E-5	45.000004	29
TCATCGG	25	3.88935E-5	45.000004	1
TTCGGCA	30	2.1643682E-6	45.000004	17
GCGGATA	25	3.88935E-5	45.000004	8
CGGTCTA	100	0.0	45.000004	30
>>END_MODULE