FastQCFastQC Report
Thu 26 May 2016
SRR1043364_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1043364_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2743382
Sequences flagged as poor quality0
Sequence length52
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1079303.9341950920433244No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC129380.47160767257348774No Hit
CCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGCT111870.4077813443406715RNA PCR Primer, Index 38 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGCTT95310.3474178951381907RNA PCR Primer, Index 38 (96% over 25bp)
GCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGCT75940.27681161427755957RNA PCR Primer, Index 27 (95% over 24bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA74280.2707606888140259No Hit
CGCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGC42830.15612116723081218RNA PCR Primer, Index 38 (95% over 23bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCG40830.1488308955880005No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC35420.12911071079419492No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC34750.126668469793853No Hit
TCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGCT33100.12065399568853336RNA PCR Primer, Index 27 (95% over 24bp)
GGGCCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCT30410.11084858032895163RNA PCR Primer, Index 27 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGATAT301.8623541E-646.08
CGTTTTT257850.045.3934441
TATACGG1550.044.5161321
ATTACGG1900.043.5789451
GTTACGG2850.043.5789451
CGGTCTA2650.043.39622530
CACTTAC34550.042.1389336
CCGCACT34700.042.02305633
ACCCGCA35250.042.0198631
CCCGCAC35350.041.96605332
CGCACTT34950.041.78826534
TAGTAGG5300.041.6603741
CTTACGG2550.041.4901921
ACTTACT35000.041.46571437
TCGGCGT12000.041.44
GCACTTA35150.041.35419535
CTTACTG34800.041.30747238
TATAAGG2900.041.2413831
TTATGAC39700.041.19143326
TATGCGG5200.041.1346171
Produced by FastQC (version 0.11.5)