##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043364_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2743382
Sequences flagged as poor quality	0
Sequence length	52
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.290896783605056	33.0	31.0	34.0	31.0	34.0
2	32.61781625745157	34.0	31.0	34.0	31.0	34.0
3	32.734844801052134	34.0	31.0	34.0	31.0	34.0
4	36.18198267685652	37.0	37.0	37.0	35.0	37.0
5	36.102939364623666	37.0	35.0	37.0	35.0	37.0
6	36.29350487828527	37.0	37.0	37.0	35.0	37.0
7	36.231685197322136	37.0	37.0	37.0	35.0	37.0
8	36.201881473305576	37.0	37.0	37.0	35.0	37.0
9	37.7897019080828	39.0	38.0	39.0	35.0	39.0
10	37.55914816091963	39.0	37.0	39.0	35.0	39.0
11	36.98687969812443	39.0	35.0	39.0	34.0	39.0
12	36.948090349794526	39.0	35.0	39.0	33.0	39.0
13	36.91436263706622	39.0	35.0	39.0	33.0	39.0
14	38.132902745589206	40.0	35.0	41.0	34.0	41.0
15	38.163224078892405	40.0	35.0	41.0	34.0	41.0
16	37.95994433148574	40.0	35.0	41.0	33.0	41.0
17	37.925756967130354	40.0	35.0	41.0	33.0	41.0
18	37.8014990256552	39.0	35.0	41.0	33.0	41.0
19	37.65140837112732	39.0	35.0	41.0	33.0	41.0
20	37.428245501355626	39.0	35.0	41.0	33.0	41.0
21	37.355300501351984	39.0	35.0	41.0	33.0	41.0
22	37.305308557102144	39.0	35.0	41.0	33.0	41.0
23	37.34288407520353	39.0	35.0	41.0	34.0	41.0
24	37.28284176246691	39.0	35.0	41.0	33.0	41.0
25	37.15866693008848	39.0	35.0	41.0	33.0	41.0
26	37.06809478227968	38.0	35.0	41.0	33.0	41.0
27	37.113953142508045	39.0	35.0	41.0	33.0	41.0
28	37.08251384604842	38.0	35.0	41.0	33.0	41.0
29	36.96700969824837	38.0	35.0	41.0	33.0	41.0
30	36.91879585125221	38.0	35.0	41.0	33.0	41.0
31	36.805384740440815	38.0	35.0	41.0	33.0	41.0
32	36.61280419569714	38.0	35.0	41.0	32.0	41.0
33	36.26740023810027	38.0	35.0	41.0	31.0	41.0
34	36.083352956314506	38.0	35.0	41.0	30.0	41.0
35	36.01976647801874	38.0	35.0	41.0	30.0	41.0
36	36.04894651929626	38.0	35.0	41.0	31.0	41.0
37	35.99916161876108	38.0	35.0	41.0	31.0	41.0
38	35.929398457815935	38.0	35.0	41.0	31.0	41.0
39	35.89867397249089	38.0	35.0	41.0	30.0	41.0
40	35.83719474721347	38.0	35.0	41.0	30.0	41.0
41	35.79943587878028	37.0	35.0	40.0	30.0	41.0
42	35.72336152967396	37.0	35.0	40.0	30.0	41.0
43	35.613879146250866	37.0	35.0	40.0	30.0	41.0
44	35.43760256500917	37.0	35.0	40.0	29.0	41.0
45	35.347129564894715	37.0	35.0	40.0	29.0	41.0
46	35.31105438469743	36.0	35.0	40.0	29.0	41.0
47	35.23883476672224	36.0	35.0	40.0	29.0	41.0
48	35.14562463411949	36.0	35.0	40.0	29.0	41.0
49	35.0979688574176	36.0	35.0	40.0	29.0	41.0
50	34.985810215274434	35.0	35.0	40.0	28.0	41.0
51	34.86414141377322	35.0	35.0	40.0	28.0	41.0
52	34.47167583661335	35.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	2.0
12	4.0
13	7.0
14	21.0
15	61.0
16	122.0
17	285.0
18	567.0
19	1119.0
20	2008.0
21	3369.0
22	5078.0
23	7709.0
24	12721.0
25	21416.0
26	31494.0
27	39539.0
28	44505.0
29	48959.0
30	54591.0
31	62456.0
32	74348.0
33	96866.0
34	242624.0
35	328818.0
36	232657.0
37	234260.0
38	358252.0
39	837497.0
40	2025.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.12453242020251	17.885114067235257	24.33128160788399	27.65907190467824
2	33.655830649905845	20.08185517000549	25.580287397088703	20.682026782999962
3	26.30712747987703	21.00473065726902	33.1973090149312	19.490832847922746
4	24.126680134228483	23.960024524473805	30.191384211167094	21.721911130130618
5	21.477286065156072	31.1912085156205	28.168370281645068	19.16313513757836
6	78.54822988559377	2.2340672935814263	16.180685008504103	3.037017812320705
7	78.79817684886757	1.9623953208120488	14.344921706127693	4.894506124192693
8	68.89674861175003	3.4656857849180316	20.225983840383876	7.411581762948069
9	45.209927017090585	14.285250832731277	23.647089614206116	16.857732535972023
10	34.99917255416854	14.48474911623682	32.77771743052918	17.738360899065462
11	31.495395099916816	12.420472249216479	35.6032080111337	20.480924639733
12	23.91497064572123	13.801395503797867	38.70842631467291	23.57520753580799
13	17.5621550334587	16.31752340723968	43.298454243703574	22.821867315598045
14	20.44768100104178	16.39217578886207	42.005816178716636	21.154327031379516
15	25.975857536427664	13.685443733318948	40.252432945904	20.08626578434939
16	22.268827308774352	18.528881504653743	37.71180972974234	21.490481456829563
17	23.156600138077746	19.314772787748844	37.20772389700013	20.320903177173285
18	22.475141996265926	26.967042868984343	33.66873443071362	16.889080704036115
19	24.508435208804315	23.316986114219603	30.87962230560673	21.294956371369352
20	23.115555908728716	28.15714326331513	31.29626132999342	17.431039497962733
21	18.836385162547543	22.09754237652649	36.46346006498548	22.602612395940486
22	21.974774202061543	21.103112873088765	33.589270469806976	23.33284245504272
23	25.22288182979986	18.774344950867214	34.81465577888898	21.18811744044395
24	25.200573598572856	16.912555378725965	31.346454850254172	26.540416172447003
25	17.341660767621864	18.509562284800293	36.94742474799354	27.20135219958431
26	16.660494236675753	17.41726088455782	36.55287524668457	29.36936963208186
27	21.087402337698506	22.43030682566263	33.24546125913198	23.23682957750689
28	23.625364604710537	23.505075122604143	29.35679391349801	23.51276635918731
29	23.212334264787042	19.445997677319454	33.17591206765955	24.165755990233954
30	29.493559409517157	17.72822742148195	30.678046294682986	22.100166874317903
31	28.577135812657517	19.984493592215742	28.085188282200583	23.35318231292616
32	28.100825914874417	18.337839936253864	27.63461304331661	25.92672110555511
33	22.796788781146773	20.14859760689543	34.795336559035526	22.25927705292227
34	20.648199922577316	25.83898268633388	30.946911512869878	22.565905878218928
35	19.589688931399273	28.149852991672326	30.700390977268206	21.560067099660202
36	24.798733825621078	25.84434103599134	27.60804000317856	21.748885135209022
37	21.562144827078402	28.2794375701233	30.870327209262143	19.28809039353615
38	23.21051169687634	23.858325235056583	29.096385410416776	23.834777657650303
39	24.86955881463099	18.808463422155572	32.34562303025973	23.976354732953705
40	20.195510504916925	19.913741505922253	33.90475697514965	25.985991014011173
41	20.158512376329654	22.738174997138568	28.920689863825018	28.18262276270676
42	20.247563044446597	16.845776490477814	32.29306017171506	30.613600293360534
43	22.323613700170082	18.203261521727562	32.06367177447399	27.40945300362837
44	21.5302498886411	20.29148693109454	32.32112771753988	25.85713546272448
45	21.629069520759415	21.219064643567684	32.78901735157554	24.362848484097366
46	21.832832613176002	20.097201191813607	32.12917486518465	25.940791329825743
47	19.14337850142634	19.1230386435429	36.05615258830159	25.677430266729168
48	22.575200974563515	17.691156390178254	32.05000251514372	27.683640120114518
49	21.172880772710474	16.313039890179347	36.01616544834077	26.49791388876941
50	19.155553255069837	16.573958712275576	37.92136858811496	26.34911944453962
51	17.70668466877744	15.21818689486189	36.50403042667773	30.57109800968294
52	16.56903777891668	16.10442876712029	40.90906771277204	26.41746574119098
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	937.0
1	4955.0
2	8973.0
3	82901.5
4	156830.0
5	88193.0
6	19556.0
7	17236.5
8	14917.0
9	14100.5
10	13284.0
11	12749.5
12	12215.0
13	11513.0
14	10133.5
15	9456.0
16	8692.0
17	7928.0
18	7661.0
19	7394.0
20	7109.0
21	6824.0
22	6323.5
23	5823.0
24	5987.0
25	6151.0
26	7336.5
27	8522.0
28	9810.5
29	11099.0
30	11483.0
31	11867.0
32	13840.5
33	15814.0
34	18452.0
35	21090.0
36	24418.5
37	27747.0
38	30141.0
39	35708.0
40	38881.0
41	47267.5
42	55654.0
43	70671.0
44	85688.0
45	111994.5
46	138301.0
47	181156.5
48	224012.0
49	255302.5
50	286593.0
51	276263.5
52	265934.0
53	240633.0
54	215332.0
55	200754.0
56	186176.0
57	174900.5
58	163625.0
59	159570.5
60	155516.0
61	151096.0
62	146676.0
63	138872.0
64	108426.0
65	85784.0
66	69476.0
67	53168.0
68	42907.5
69	32647.0
70	27620.5
71	22594.0
72	21953.5
73	21313.0
74	16947.5
75	12582.0
76	9325.0
77	6068.0
78	4708.0
79	3348.0
80	2499.0
81	1650.0
82	1099.0
83	548.0
84	397.5
85	247.0
86	188.0
87	129.0
88	83.5
89	30.0
90	22.0
91	15.5
92	9.0
93	5.5
94	2.0
95	3.0
96	4.0
97	2.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	2743382.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.579391500884203
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.43955721267932	19.70259517018299
2	11.567345349964974	6.3804069206523275
3	4.659739957884967	3.8553837777246946
4	2.4046463892985357	2.652747367666077
5	1.376684339714696	1.8984058189063937
6	0.9192936731902882	1.5212136070320512
7	0.6951040648259388	1.3419382996383395
8	0.5397007405734489	1.1907694414073828
9	0.4406678913714614	1.0938017068202384
>10	5.307012056726604	32.551477586066554
>50	0.45094518473573303	8.225531750660632
>100	0.1848330495606206	9.394419180401927
>500	0.008628952438314097	1.6834428033281266
>1k	0.005044618348552856	2.774055987876587
>5k	3.9825934330680446E-4	0.8989467409768482
>10k+	3.9825934330680446E-4	4.834863840658889
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	107930	3.9341950920433244	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	12938	0.47160767257348774	No Hit
CCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGCT	11187	0.4077813443406715	RNA PCR Primer, Index 38 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGCTT	9531	0.3474178951381907	RNA PCR Primer, Index 38 (96% over 25bp)
GCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGCT	7594	0.27681161427755957	RNA PCR Primer, Index 27 (95% over 24bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA	7428	0.2707606888140259	No Hit
CGCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGC	4283	0.15612116723081218	RNA PCR Primer, Index 38 (95% over 23bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCG	4083	0.1488308955880005	No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	3542	0.12911071079419492	No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	3475	0.126668469793853	No Hit
TCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGCT	3310	0.12065399568853336	RNA PCR Primer, Index 27 (95% over 24bp)
GGGCCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCT	3041	0.11084858032895163	RNA PCR Primer, Index 27 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.3564213806170632	0.0
2	0.0	0.0	0.0	1.2475477348761492	0.0
3	0.0	0.0	0.0	1.9845212952479823	0.0
4	0.0	0.0	0.0	2.4725320790177965	0.0
5	0.0	0.0	0.0	5.085292533085076	0.0
6	0.0	0.0	0.0	5.878474087822986	0.0
7	0.0	0.0	0.0	8.371418927440656	0.0
8	0.0	0.0	0.0	11.505688963476468	0.0
9	0.0	0.0	0.0	14.279856031715598	0.0
10	0.0	0.0	0.0	15.959315910070124	0.0
11	0.0	0.0	0.0	17.392145898748332	0.0
12	0.0	0.0	0.0	18.424229655221183	0.0
13	0.0	0.0	0.0	18.970963577073846	0.0
14	0.0	0.0	0.0	19.316741161092402	0.0
15	3.645135821405841E-5	0.0	0.0	20.180966412989516	0.0
16	3.645135821405841E-5	0.0	0.0	21.126186582838262	0.0
17	3.645135821405841E-5	0.0	0.0	22.25257000301088	3.645135821405841E-5
18	7.290271642811682E-5	0.0	0.0	23.023953645536785	3.645135821405841E-5
19	7.290271642811682E-5	0.0	0.0	23.868859677580446	3.645135821405841E-5
20	7.290271642811682E-5	0.0	0.0	24.75539316070456	3.645135821405841E-5
21	7.290271642811682E-5	0.0	0.0	25.584187692417608	3.645135821405841E-5
22	7.290271642811682E-5	0.0	0.0	26.372120251572692	3.645135821405841E-5
23	1.0935407464217524E-4	0.0	0.0	27.11350442628843	3.645135821405841E-5
24	1.0935407464217524E-4	0.0	0.0	27.751257389601594	3.645135821405841E-5
25	1.0935407464217524E-4	0.0	0.0	28.329485284951204	3.645135821405841E-5
26	1.4580543285623365E-4	0.0	0.0	28.88263464584954	3.645135821405841E-5
27	1.4580543285623365E-4	0.0	0.0	29.50391159524995	3.645135821405841E-5
28	1.4580543285623365E-4	0.0	0.0	30.091616843735213	3.645135821405841E-5
29	1.4580543285623365E-4	0.0	0.0	30.697073903670724	3.645135821405841E-5
30	1.4580543285623365E-4	0.0	0.0	31.27005280343751	3.645135821405841E-5
31	1.4580543285623365E-4	0.0	0.0	31.839641726890385	3.645135821405841E-5
32	1.8225679107029206E-4	0.0	0.0	32.51898568992579	3.645135821405841E-5
33	1.8225679107029206E-4	0.0	0.0	33.148792257148294	3.645135821405841E-5
34	1.8225679107029206E-4	0.0	0.0	33.78869585059609	3.645135821405841E-5
35	1.8225679107029206E-4	0.0	0.0	34.36663213507998	3.645135821405841E-5
36	1.8225679107029206E-4	0.0	0.0	34.95845638704344	3.645135821405841E-5
37	2.1870814928435047E-4	0.0	0.0	35.83828282025617	3.645135821405841E-5
38	2.1870814928435047E-4	0.0	0.0	36.703237099317555	3.645135821405841E-5
39	2.1870814928435047E-4	0.0	0.0	37.75598877589778	3.645135821405841E-5
40	2.1870814928435047E-4	0.0	0.0	38.69373641731264	3.645135821405841E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGATAT	30	1.8623541E-6	46.0	8
CGTTTTT	25785	0.0	45.393444	1
TATACGG	155	0.0	44.516132	1
ATTACGG	190	0.0	43.578945	1
GTTACGG	285	0.0	43.578945	1
CGGTCTA	265	0.0	43.396225	30
CACTTAC	3455	0.0	42.13893	36
CCGCACT	3470	0.0	42.023056	33
ACCCGCA	3525	0.0	42.01986	31
CCCGCAC	3535	0.0	41.966053	32
CGCACTT	3495	0.0	41.788265	34
TAGTAGG	530	0.0	41.660374	1
CTTACGG	255	0.0	41.490192	1
ACTTACT	3500	0.0	41.465714	37
TCGGCGT	1200	0.0	41.4	4
GCACTTA	3515	0.0	41.354195	35
CTTACTG	3480	0.0	41.307472	38
TATAAGG	290	0.0	41.241383	1
TTATGAC	3970	0.0	41.191433	26
TATGCGG	520	0.0	41.134617	1
>>END_MODULE