FastQCFastQC Report
Thu 26 May 2016
SRR1043363_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1043363_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2704221
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1028053.801649347446085No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC125930.4656793952861101No Hit
CCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGC106720.39464230179412113RNA PCR Primer, Index 38 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGCT93570.34601461936727806RNA PCR Primer, Index 38 (95% over 24bp)
GCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGC77840.28784629658596694RNA PCR Primer, Index 27 (95% over 23bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG62000.22927120231667455No Hit
CGCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTG43390.16045286239549209RNA PCR Primer, Index 38 (95% over 22bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCC38940.14399710674534366No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC33420.12358457389392362No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC33300.1231408231797623No Hit
TCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGC32350.11962779669265197RNA PCR Primer, Index 27 (95% over 23bp)
GGGCCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTC30490.11274966062315174No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG253.8922102E-545.00000414
TTGCGAC207.034981E-445.00000411
CGTAAAA207.034981E-445.00000431
TGTCGAT207.034981E-445.00000416
CGTTTTT244550.044.337561
CAACCGG900.042.51
TATACGG1750.042.428571
ATAGCGG3400.042.352941
ATTACGG2300.042.0652161
GCGATCG750.042.08
CACTTAC34250.041.71532436
TATGCGG5300.041.6037751
TCACGAC3750.041.424
CTAACGG2200.040.909091
ATTGCGG5450.040.8715631
CGCACTT34950.040.81545334
ACCCGCA35850.040.73221631
GCACTTA35000.040.69285635
CCGCACT35200.040.58948533
CCCGCAC36150.040.39418832