Basic Statistics
Measure | Value |
---|---|
Filename | SRR1043363_1.fastq.gz |
File type | Conventional base calls |
Encoding | Illumina 1.5 |
Total Sequences | 2704221 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 49 |
Per base sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 102805 | 3.801649347446085 | No Hit |
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC | 12593 | 0.4656793952861101 | No Hit |
CCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGC | 10672 | 0.39464230179412113 | RNA PCR Primer, Index 38 (95% over 23bp) |
CTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGCT | 9357 | 0.34601461936727806 | RNA PCR Primer, Index 38 (95% over 24bp) |
GCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGC | 7784 | 0.28784629658596694 | RNA PCR Primer, Index 27 (95% over 23bp) |
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG | 6200 | 0.22927120231667455 | No Hit |
CGCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTG | 4339 | 0.16045286239549209 | RNA PCR Primer, Index 38 (95% over 22bp) |
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCC | 3894 | 0.14399710674534366 | No Hit |
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC | 3342 | 0.12358457389392362 | No Hit |
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC | 3330 | 0.1231408231797623 | No Hit |
TCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGC | 3235 | 0.11962779669265197 | RNA PCR Primer, Index 27 (95% over 23bp) |
GGGCCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTC | 3049 | 0.11274966062315174 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
TATCACG | 25 | 3.8922102E-5 | 45.000004 | 14 |
TTGCGAC | 20 | 7.034981E-4 | 45.000004 | 11 |
CGTAAAA | 20 | 7.034981E-4 | 45.000004 | 31 |
TGTCGAT | 20 | 7.034981E-4 | 45.000004 | 16 |
CGTTTTT | 24455 | 0.0 | 44.33756 | 1 |
CAACCGG | 90 | 0.0 | 42.5 | 1 |
TATACGG | 175 | 0.0 | 42.42857 | 1 |
ATAGCGG | 340 | 0.0 | 42.35294 | 1 |
ATTACGG | 230 | 0.0 | 42.065216 | 1 |
GCGATCG | 75 | 0.0 | 42.0 | 8 |
CACTTAC | 3425 | 0.0 | 41.715324 | 36 |
TATGCGG | 530 | 0.0 | 41.603775 | 1 |
TCACGAC | 375 | 0.0 | 41.4 | 24 |
CTAACGG | 220 | 0.0 | 40.90909 | 1 |
ATTGCGG | 545 | 0.0 | 40.871563 | 1 |
CGCACTT | 3495 | 0.0 | 40.815453 | 34 |
ACCCGCA | 3585 | 0.0 | 40.732216 | 31 |
GCACTTA | 3500 | 0.0 | 40.692856 | 35 |
CCGCACT | 3520 | 0.0 | 40.589485 | 33 |
CCCGCAC | 3615 | 0.0 | 40.394188 | 32 |