FastQCFastQC Report
Thu 26 May 2016
SRR1043362_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1043362_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2319469
Sequences flagged as poor quality0
Sequence length52
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT848723.659113357410683No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC184950.7973807798250375No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA120180.5181358319511923No Hit
CCTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGCCGTCTTCTGCT62470.26932888518880826No Hit
CTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGCCGTCTTCTGCTT57390.24742732064968317Illumina Single End Adapter 2 (95% over 21bp)
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC54210.233717286154719No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC47050.20284815188303876No Hit
GCTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGCCGTCTTCTGCT43930.189396797284206No Hit
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC35450.15283670529763493No Hit
CGCTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGCCGTCTTCTGC32420.1397733705429993No Hit
TGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC27640.11916520548453115No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAA23350.10066959291113614No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATACGG1450.046.0000041
TACGGTC206.31279E-446.023
ACGTATA253.418239E-546.046
CGAAGTA206.31279E-446.020
CGTTTTT207400.045.2569921
CCGGTAT1400.042.71428742
CGGTCTA3500.042.71428330
ACGGGAT5300.042.5283054
ACAACGG1300.042.461541
GCACTTA47600.041.8445435
TCACGAC3850.041.8181824
CCGCACT47700.041.75681333
CGCACTT47950.041.68300234
ACCCGCA48200.041.65767731
CACTTAC47850.041.62591636
CCCGCAC48550.041.35736532
CTTACTG47800.041.18828638
TACGGGA3300.041.1212123
CACGACG3750.041.09333425
ACTTACT48300.040.9523837
Produced by FastQC (version 0.11.5)