##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043362_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2319469
Sequences flagged as poor quality	0
Sequence length	52
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.29466183855012	33.0	31.0	34.0	31.0	34.0
2	32.637917557854834	34.0	31.0	34.0	31.0	34.0
3	32.7899769300646	34.0	31.0	34.0	31.0	34.0
4	36.220128400077776	37.0	37.0	37.0	35.0	37.0
5	36.165852184271486	37.0	37.0	37.0	35.0	37.0
6	36.28752701588165	37.0	37.0	37.0	35.0	37.0
7	36.22545117007384	37.0	37.0	37.0	35.0	37.0
8	36.219520071188704	37.0	37.0	37.0	35.0	37.0
9	37.81536161940513	39.0	38.0	39.0	35.0	39.0
10	37.59553501253951	39.0	37.0	39.0	35.0	39.0
11	37.24866898415112	39.0	37.0	39.0	35.0	39.0
12	37.257237324577304	39.0	37.0	39.0	34.0	39.0
13	37.252641445089374	39.0	37.0	39.0	34.0	39.0
14	38.56715179206965	40.0	37.0	41.0	35.0	41.0
15	38.62961824452062	40.0	38.0	41.0	35.0	41.0
16	38.4940609251514	40.0	37.0	41.0	34.0	41.0
17	38.487878044500704	40.0	37.0	41.0	34.0	41.0
18	38.309425131355496	40.0	37.0	41.0	34.0	41.0
19	38.105174934435425	40.0	37.0	41.0	34.0	41.0
20	37.85965408462023	40.0	35.0	41.0	34.0	41.0
21	37.79698801751608	40.0	35.0	41.0	34.0	41.0
22	37.743878016908184	40.0	35.0	41.0	34.0	41.0
23	37.75013074113084	40.0	35.0	41.0	34.0	41.0
24	37.654453239081874	40.0	35.0	41.0	34.0	41.0
25	37.54864669456673	39.0	35.0	41.0	34.0	41.0
26	37.45042335120668	39.0	35.0	41.0	33.0	41.0
27	37.487918139884606	39.0	35.0	41.0	33.0	41.0
28	37.41913127530482	39.0	35.0	41.0	33.0	41.0
29	37.351912873161915	39.0	35.0	41.0	33.0	41.0
30	37.28205722947796	39.0	35.0	41.0	33.0	41.0
31	37.16766725487601	39.0	35.0	41.0	33.0	41.0
32	36.9311385493835	39.0	35.0	41.0	33.0	41.0
33	36.59053559241361	39.0	35.0	41.0	31.0	41.0
34	36.374864678079334	39.0	35.0	41.0	31.0	41.0
35	36.22486396670962	39.0	35.0	41.0	31.0	41.0
36	36.16328737310134	39.0	35.0	41.0	31.0	41.0
37	36.08110778803252	38.0	35.0	41.0	31.0	41.0
38	35.94109384518612	38.0	35.0	41.0	30.0	41.0
39	35.90301875127454	38.0	35.0	41.0	30.0	41.0
40	35.804253042398926	38.0	35.0	41.0	30.0	41.0
41	35.748252725084924	38.0	35.0	41.0	30.0	41.0
42	35.64975561216813	37.0	35.0	41.0	30.0	41.0
43	35.52727456154835	37.0	35.0	40.0	29.0	41.0
44	35.335330629553575	37.0	35.0	40.0	29.0	41.0
45	35.20516721715185	37.0	35.0	40.0	28.0	41.0
46	35.13810660974559	36.0	35.0	40.0	28.0	41.0
47	35.02823146159746	36.0	35.0	40.0	28.0	41.0
48	34.89858195992272	36.0	35.0	40.0	28.0	41.0
49	34.77071476273233	35.0	35.0	39.0	28.0	41.0
50	34.600111491035236	35.0	35.0	39.0	27.0	41.0
51	34.43651283979221	35.0	34.0	39.0	27.0	41.0
52	34.036031091598986	35.0	34.0	39.0	26.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	5.0
12	3.0
13	5.0
14	17.0
15	46.0
16	79.0
17	207.0
18	430.0
19	862.0
20	1628.0
21	2737.0
22	4244.0
23	6754.0
24	11114.0
25	18846.0
26	28824.0
27	35027.0
28	37745.0
29	38758.0
30	41180.0
31	46660.0
32	56276.0
33	73858.0
34	164414.0
35	236716.0
36	213310.0
37	239722.0
38	356054.0
39	702453.0
40	1494.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.663397527623783	17.818561058587115	23.72452488047911	27.79351653330999
2	33.15293284799236	20.358883865229497	24.675302838710067	21.81288044806807
3	27.319270057069094	21.225289064005597	31.573519628846086	19.88192125007922
4	23.915516870456127	24.14798387044621	29.253419640443568	22.683079618654098
5	21.871902577702052	32.54042196726923	26.889559636278822	18.698115818749894
6	80.68204403680325	2.198261757324629	14.50426800271959	2.615426203152532
7	80.5380455612901	2.0352071961298037	13.327964288378071	4.098782954202018
8	72.01005919889423	3.4996803147616973	18.016408065811614	6.473852420532459
9	43.707288176733556	16.529516022848334	22.306139896674626	17.457055903743488
10	29.33317927508408	16.64208489098151	32.228626465798854	21.796109368135554
11	25.31566492158335	13.82014590408408	37.774119852431745	23.090069321900835
12	21.51742489336999	15.221026881583674	39.895467453973296	23.366080771073033
13	17.77217113054755	18.62797045358226	41.45737666681469	22.142481749055495
14	17.881851406507266	17.892543508880696	40.9955468255881	23.230058259023938
15	21.558770563434994	14.573033741774518	40.54458153999902	23.323614154791464
16	21.153720959409245	18.04106888257614	36.46692411064774	24.338286047366875
17	21.97451226983417	19.722531320746256	35.483983618664446	22.818972790755126
18	22.515110139432775	24.77610177156927	32.35270658930988	20.356081499688074
19	23.954361968191858	20.817825114282623	31.096901920223985	24.130910997301537
20	22.982587825058236	24.606062853178894	32.51718388993343	19.894165431829443
21	20.941775897845584	19.159126506972072	34.02451164469109	25.87458595049125
22	22.55943062830329	21.415418787662176	31.618702384037036	24.4064481999975
23	22.946803772760056	19.685324529019358	34.51229570216287	22.855575996057716
24	25.57331009813022	18.286857897216994	31.38144980596852	24.758382198684266
25	20.225361925509674	19.515285610628986	33.96040214376653	26.298950320094818
26	19.865667529938964	18.83008567909293	33.99605685611664	27.30818993485147
27	23.70952144650349	20.604284860026155	33.156209460010025	22.529984233460333
28	23.73202659746692	21.764033061015258	29.915597061223924	24.588343280293895
29	25.02051116009742	19.149986483975425	32.60034947653968	23.22915287938748
30	29.598584848514896	19.35063585674135	28.7853383683938	22.265440926349953
31	29.291316245226817	19.02913985916604	27.19842343225971	24.48112046334743
32	29.353787440142547	19.86131308502075	26.509645095493838	24.275254379342858
33	26.340770236636057	19.63729629497096	30.461540982009243	23.560392486383737
34	24.515783569429036	23.320337542773796	28.576109445739522	23.587769442057642
35	26.466014419679677	24.3869609811556	26.210740475514005	22.936284123650715
36	26.23734139149952	24.530183416980353	26.789493629791995	22.442981561728136
37	24.759891164745035	26.771041130534616	26.61419488684695	21.854872817873403
38	22.81673089832199	22.48549129132573	27.37544670784563	27.322331102506652
39	23.85645162750612	19.9104191519697	32.48933268778328	23.743796532740898
40	21.051154380593147	19.635528649014063	34.371918745195565	24.941398225197233
41	22.30437225071773	23.18539286362525	28.373778653648746	26.136456232008275
42	21.292287157103633	18.26918143764801	30.964716493300838	29.47381491194752
43	23.66709794353794	19.33295939717237	31.590333822094625	25.409608837195062
44	24.701645074799448	20.8768472439166	30.850121299314626	23.571386381969322
45	25.17149399280611	20.883960941060216	29.65402857291906	24.29051649321461
46	24.191010959836063	20.51887738098677	29.846357075692755	25.443754583484406
47	21.673451984053248	22.0318529801433	31.908639434284314	24.38605560151914
48	23.41833411009158	19.973752613205868	29.713180042501108	26.89473323420145
49	22.45375989073361	18.144109707868484	33.46416787635446	25.937962525043446
50	21.956145997208846	18.38170719246517	34.60572225798232	25.05642455234366
51	21.05425853934672	16.83919897183364	33.26739870203051	28.83914378678913
52	18.97348056818177	18.589685828954817	36.216392631244474	26.220440971618935
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	592.0
1	3618.0
2	6644.0
3	59531.0
4	112418.0
5	64318.5
6	16219.0
7	14703.0
8	13187.0
9	12915.0
10	12643.0
11	12335.5
12	12028.0
13	11440.0
14	10189.5
15	9527.0
16	8857.5
17	8188.0
18	7738.0
19	7288.0
20	6884.5
21	6481.0
22	6522.5
23	6564.0
24	6621.5
25	6679.0
26	7578.5
27	8478.0
28	9159.5
29	9841.0
30	11136.0
31	12431.0
32	13885.0
33	15339.0
34	18749.5
35	22160.0
36	23836.0
37	25512.0
38	29020.5
39	35015.0
40	37501.0
41	42309.5
42	47118.0
43	56902.5
44	66687.0
45	76187.0
46	85687.0
47	103012.0
48	120337.0
49	155253.5
50	190170.0
51	207476.0
52	224782.0
53	209757.0
54	194732.0
55	181006.0
56	167280.0
57	156673.0
58	146066.0
59	140601.0
60	135136.0
61	132539.0
62	129942.0
63	127684.0
64	106859.0
65	88292.0
66	76358.0
67	64424.0
68	54051.5
69	43679.0
70	37704.5
71	31730.0
72	31123.5
73	30517.0
74	23801.0
75	17085.0
76	12721.5
77	8358.0
78	6503.5
79	4649.0
80	3508.5
81	2368.0
82	1667.0
83	966.0
84	739.5
85	513.0
86	356.5
87	200.0
88	129.0
89	68.0
90	78.0
91	74.5
92	71.0
93	41.5
94	12.0
95	8.0
96	4.0
97	2.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	2319469.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.021761649650223
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.88957029062327	20.236481184379485
2	9.8965776432451	5.348459244460524
3	3.6085937035272506	2.925316768414257
4	1.7682699631022731	1.9112707790074162
5	1.0466021814737925	1.414051734489439
6	0.7300475952471825	1.1836303267001809
7	0.5637205187306331	1.0662905045909448
8	0.466841753605739	1.009190927523121
9	0.42752950842819515	1.0397340427444934
>10	5.774097356694969	36.490905768719536
>50	0.676876583944638	11.892700959446987
>100	0.13890807385845702	6.257190869581798
>500	0.007065615724937552	1.2876615388658241
>1k	0.004335718740302589	2.1749872611326224
>5k	4.817465267002877E-4	0.755326854491572
>10k+	4.817465267002877E-4	5.006801235451832
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	84872	3.659113357410683	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	18495	0.7973807798250375	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA	12018	0.5181358319511923	No Hit
CCTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGCCGTCTTCTGCT	6247	0.26932888518880826	No Hit
CTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGCCGTCTTCTGCTT	5739	0.24742732064968317	Illumina Single End Adapter 2 (95% over 21bp)
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	5421	0.233717286154719	No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	4705	0.20284815188303876	No Hit
GCTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGCCGTCTTCTGCT	4393	0.189396797284206	No Hit
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	3545	0.15283670529763493	No Hit
CGCTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGCCGTCTTCTGC	3242	0.1397733705429993	No Hit
TGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	2764	0.11916520548453115	No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAA	2335	0.10066959291113614	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.2576882898628953	0.0
2	0.0	0.0	0.0	0.8542041303419016	0.0
3	0.0	0.0	0.0	1.4220065023503223	0.0
4	0.0	0.0	0.0	1.804852748624793	0.0
5	0.0	0.0	0.0	3.767112214045542	0.0
6	0.0	0.0	0.0	4.401740225887908	0.0
7	0.0	0.0	0.0	6.427290039228806	0.0
8	0.0	0.0	0.0	8.664655574185298	0.0
9	0.0	0.0	0.0	10.699431637154884	0.0
10	0.0	0.0	0.0	11.829992123197163	0.0
11	4.311331602189984E-5	0.0	0.0	12.795342382243522	0.0
12	4.311331602189984E-5	0.0	0.0	13.503133691375051	0.0
13	4.311331602189984E-5	0.0	0.0	13.860111948036382	0.0
14	4.311331602189984E-5	0.0	0.0	14.07994674643205	0.0
15	4.311331602189984E-5	0.0	0.0	14.718670523296495	0.0
16	4.311331602189984E-5	0.0	0.0	15.411199718556272	0.0
17	4.311331602189984E-5	0.0	0.0	16.310586604089124	0.0
18	4.311331602189984E-5	0.0	0.0	16.80263887984707	0.0
19	4.311331602189984E-5	0.0	0.0	17.3397445708479	0.0
20	4.311331602189984E-5	0.0	0.0	17.9407873095092	0.0
21	4.311331602189984E-5	0.0	0.0	18.542476747910836	0.0
22	4.311331602189984E-5	0.0	0.0	19.13554352310809	0.0
23	4.311331602189984E-5	0.0	0.0	19.661181072047093	0.0
24	8.622663204379967E-5	0.0	0.0	20.083044869321384	0.0
25	8.622663204379967E-5	0.0	0.0	20.49434590417031	0.0
26	8.622663204379967E-5	0.0	0.0	20.91508875522803	0.0
27	1.7245326408759935E-4	0.0	0.0	21.35829364393316	0.0
28	1.7245326408759935E-4	0.0	0.0	21.804602691391867	0.0
29	1.7245326408759935E-4	0.0	0.0	22.258930815630645	0.0
30	1.7245326408759935E-4	0.0	0.0	22.68385565834249	0.0
31	1.7245326408759935E-4	0.0	0.0	23.122576762181343	0.0
32	1.7245326408759935E-4	0.0	0.0	23.59678012510622	0.0
33	1.7245326408759935E-4	0.0	0.0	24.031060557394817	0.0
34	1.7245326408759935E-4	0.0	0.0	24.5312612498809	0.0
35	1.7245326408759935E-4	0.0	0.0	24.93725934685913	0.0
36	1.7245326408759935E-4	0.0	0.0	25.37684271701842	0.0
37	2.155665801094992E-4	0.0	0.0	25.906834710875636	0.0
38	2.155665801094992E-4	0.0	0.0	26.481578326763582	0.0
39	2.155665801094992E-4	0.0	0.0	27.146256319873213	0.0
40	2.155665801094992E-4	0.0	0.0	27.725699287207547	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AATACGG	145	0.0	46.000004	1
TACGGTC	20	6.31279E-4	46.0	23
ACGTATA	25	3.418239E-5	46.0	46
CGAAGTA	20	6.31279E-4	46.0	20
CGTTTTT	20740	0.0	45.256992	1
CCGGTAT	140	0.0	42.714287	42
CGGTCTA	350	0.0	42.714283	30
ACGGGAT	530	0.0	42.528305	4
ACAACGG	130	0.0	42.46154	1
GCACTTA	4760	0.0	41.84454	35
TCACGAC	385	0.0	41.81818	24
CCGCACT	4770	0.0	41.756813	33
CGCACTT	4795	0.0	41.683002	34
ACCCGCA	4820	0.0	41.657677	31
CACTTAC	4785	0.0	41.625916	36
CCCGCAC	4855	0.0	41.357365	32
CTTACTG	4780	0.0	41.188286	38
TACGGGA	330	0.0	41.121212	3
CACGACG	375	0.0	41.093334	25
ACTTACT	4830	0.0	40.95238	37
>>END_MODULE