##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043361_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2276402
Sequences flagged as poor quality	0
Sequence length	51
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.29453365442483	33.0	31.0	34.0	31.0	34.0
2	32.58680408820586	34.0	31.0	34.0	31.0	34.0
3	32.709792470749896	34.0	31.0	34.0	31.0	34.0
4	36.190913994979795	37.0	37.0	37.0	35.0	37.0
5	36.18076640241926	37.0	37.0	37.0	35.0	37.0
6	36.31255331878992	37.0	37.0	37.0	35.0	37.0
7	36.26162821856597	37.0	37.0	37.0	35.0	37.0
8	36.17481490527596	37.0	37.0	37.0	35.0	37.0
9	37.77687113260312	39.0	38.0	39.0	35.0	39.0
10	37.53941043805092	39.0	37.0	39.0	35.0	39.0
11	37.235479937199145	39.0	37.0	39.0	35.0	39.0
12	37.250593700058246	39.0	37.0	39.0	34.0	39.0
13	37.25943704143644	39.0	37.0	39.0	35.0	39.0
14	38.568292419353	40.0	38.0	41.0	35.0	41.0
15	38.63893591729405	40.0	38.0	41.0	35.0	41.0
16	38.54410205227372	40.0	38.0	41.0	34.0	41.0
17	38.50697504219378	40.0	37.0	41.0	34.0	41.0
18	38.352345060318875	40.0	37.0	41.0	34.0	41.0
19	38.16972353740684	40.0	37.0	41.0	34.0	41.0
20	37.94555399266035	40.0	35.0	41.0	34.0	41.0
21	37.88562389244079	40.0	35.0	41.0	34.0	41.0
22	37.85203096816819	40.0	35.0	41.0	34.0	41.0
23	37.82278613355638	40.0	35.0	41.0	34.0	41.0
24	37.72523042942328	40.0	35.0	41.0	34.0	41.0
25	37.692656657303935	40.0	35.0	41.0	34.0	41.0
26	37.65481536213727	39.0	35.0	41.0	34.0	41.0
27	37.63574140244122	39.0	35.0	41.0	34.0	41.0
28	37.51961340747373	39.0	35.0	41.0	34.0	41.0
29	37.47353411216472	39.0	35.0	41.0	34.0	41.0
30	37.35911275776423	39.0	35.0	41.0	33.0	41.0
31	37.23593240561201	39.0	35.0	41.0	33.0	41.0
32	37.019074398985765	39.0	35.0	41.0	33.0	41.0
33	36.66630234905786	39.0	35.0	41.0	32.0	41.0
34	36.40868440635705	39.0	35.0	41.0	31.0	41.0
35	36.254265722838056	39.0	35.0	41.0	31.0	41.0
36	36.184104125721205	39.0	35.0	41.0	31.0	41.0
37	36.051841458582444	38.0	35.0	41.0	31.0	41.0
38	35.91847397779478	38.0	35.0	41.0	30.0	41.0
39	35.841246844801574	38.0	35.0	41.0	30.0	41.0
40	35.718786049212746	38.0	35.0	40.0	30.0	41.0
41	35.618779108435156	38.0	35.0	40.0	29.0	41.0
42	35.500630820039696	37.0	35.0	40.0	29.0	41.0
43	35.36147789362336	37.0	35.0	40.0	29.0	41.0
44	35.21538638606011	37.0	35.0	40.0	28.0	41.0
45	35.123686413911074	36.0	35.0	40.0	28.0	41.0
46	35.00608723766716	36.0	35.0	40.0	28.0	41.0
47	34.839887243114354	36.0	35.0	40.0	27.0	41.0
48	34.74116478548165	36.0	35.0	40.0	27.0	41.0
49	34.60155060485802	35.0	34.0	39.0	27.0	41.0
50	34.45896594713939	35.0	34.0	39.0	27.0	41.0
51	34.05012866795935	35.0	34.0	39.0	26.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	2.0
13	6.0
14	17.0
15	34.0
16	107.0
17	188.0
18	413.0
19	865.0
20	1505.0
21	2442.0
22	3903.0
23	5867.0
24	9459.0
25	16505.0
26	26516.0
27	34488.0
28	37410.0
29	38108.0
30	40312.0
31	45809.0
32	54226.0
33	71918.0
34	160756.0
35	233832.0
36	202785.0
37	237668.0
38	364845.0
39	686313.0
40	103.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.89568538421597	17.94182222647845	23.791711657255615	27.370780732049965
2	33.013632917208824	20.353391009145135	24.731308442006288	21.901667631639754
3	27.38470621621313	21.34882151746484	31.370162212122466	19.896310054199564
4	24.0281813141967	24.27703894127663	28.931357466739176	22.76342227778749
5	22.066752708880067	32.59050905771476	26.617662433963773	18.7250757994414
6	80.91163160109683	2.165215106997797	14.299802934631053	2.6233503572743304
7	80.81538322317411	2.019283061603355	13.072076021721998	4.093257693500533
8	72.29101011157081	3.4378813583892476	17.7878511791854	6.48325735085455
9	43.93134428804754	16.49985371652283	22.00670180398717	17.56210019144246
10	29.657635162857876	16.64692791519248	31.858081305498764	21.837355616450875
11	25.509026964481667	13.77924461496695	37.61747705370141	23.094251366849967
12	21.75077161239535	15.184488504227284	39.694658500563605	23.37008138281376
13	17.881990966446175	18.595792834481784	41.35284541131136	22.169370787760688
14	18.008550335134128	17.936023602158144	40.849331532831194	23.206094529876534
15	21.724238513232724	14.533461137356232	40.38952698161397	23.352773367797074
16	21.280863397589705	17.940196854509878	36.3223191685827	24.45662057931771
17	22.151491696106397	19.64758421403601	35.31788322097766	22.883040868879924
18	22.706138898138377	24.862787855572083	32.135712409319616	20.29536083696992
19	24.09618336304396	20.932418790705682	30.833262314828403	24.138135531421952
20	23.11850015946217	24.744618920559724	32.19835512356781	19.9385257964103
21	20.995764368507846	19.289738807117548	33.827417125797645	25.887079698576965
22	22.647318004464942	21.41559355509264	31.346176993343004	24.590911447099415
23	23.17319172975599	19.691732831020182	34.19396046919657	22.94111497002726
24	25.712989182051327	18.23742027989784	31.233674895734588	24.815915642316252
25	20.18567019357741	19.513600849059173	33.85245663990806	26.448272317455352
26	19.936329347804122	18.67899430768379	33.94281853556622	27.441857808945873
27	23.75713955619438	20.629792101746528	32.976556864736544	22.636511477322546
28	23.78863662920697	21.770627507795197	29.756651066024364	24.68408479697347
29	25.08915384892475	19.159884765520324	32.42410611131075	23.326855274244178
30	29.782964520326377	19.30647574549662	28.52198337551979	22.388576358657215
31	29.531207581086292	18.97362592371646	26.933160311755127	24.56200618344212
32	29.519478545529303	19.8171061174608	26.291270171085774	24.37214516592412
33	26.635234022813194	19.503233611638016	30.236004009836577	23.625528355712216
34	24.67951618387262	23.302386836771362	28.389186092790293	23.62891088656573
35	26.562268000115974	24.363315442527288	25.961275732493643	23.113140824863095
36	26.212769097900985	24.533408422589684	26.671870785564238	22.581951693945093
37	24.903114651981504	26.72111516331474	26.551285757085086	21.82448442761867
38	23.00129766183653	22.461366665465942	27.251469643762395	27.285866028935136
39	23.948450229792453	19.822465452059873	32.34380395026889	23.885280367878785
40	21.165725561653872	19.545141851043883	34.279534106893244	25.009598480408997
41	22.419150923255206	23.12719809594263	28.252303415653294	26.201347565148865
42	21.43057333458677	18.143675853386178	30.867658700001144	29.558092112025907
43	23.87407847998728	19.11138718029592	31.549743850163548	25.464790489553252
44	24.80040871515664	20.74866390031286	30.760867368768785	23.690060015761716
45	25.272030159875104	20.78178634529402	29.656976228276026	24.28920726655485
46	24.369509427596707	20.46220307309517	29.79785644187626	25.37043105743186
47	21.89986654378269	21.744533698353806	31.876663260707026	24.47893649715648
48	23.59429485653237	19.83054838293061	29.65767909183	26.91747766870702
49	22.621663484744786	17.979469355588336	33.368974372716245	26.029892786950636
50	22.09600940431435	18.357302444823016	34.49799288526367	25.04869526559896
51	21.173544918691867	16.879180390809708	33.07754957164859	28.869725118849832
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	535.0
1	3403.5
2	6272.0
3	56795.0
4	107318.0
5	61871.0
6	16424.0
7	14804.5
8	13185.0
9	12741.0
10	12297.0
11	11931.5
12	11566.0
13	10935.5
14	10305.0
15	9564.5
16	8824.0
17	8183.5
18	7543.0
19	7268.5
20	6994.0
21	6483.0
22	5972.0
23	6155.5
24	6339.0
25	6482.0
26	7448.0
27	8271.0
28	8899.5
29	9528.0
30	11493.5
31	13459.0
32	15197.0
33	16935.0
34	19460.0
35	21985.0
36	25174.5
37	28364.0
38	30945.0
39	33526.0
40	37650.0
41	41774.0
42	48533.0
43	55292.0
44	62661.0
45	70030.0
46	80568.5
47	91107.0
48	122183.5
49	153260.0
50	183277.5
51	213295.0
52	216190.0
53	219085.0
54	200285.0
55	181485.0
56	168777.5
57	156070.0
58	148374.5
59	140679.0
60	135167.0
61	129655.0
62	127366.5
63	125078.0
64	118563.0
65	112048.0
66	93829.0
67	75610.0
68	63268.0
69	50926.0
70	43745.0
71	36564.0
72	33291.0
73	30018.0
74	25493.0
75	15823.5
76	10679.0
77	7967.5
78	5256.0
79	4070.5
80	2885.0
81	2021.5
82	1158.0
83	924.5
84	691.0
85	498.0
86	305.0
87	189.5
88	74.0
89	79.0
90	84.0
91	65.5
92	47.0
93	28.0
94	9.0
95	5.5
96	2.0
97	1.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2276402.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.691025015879816
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.23492128641789	19.814061411076487
2	10.143176580990787	5.414635597274228
3	3.6002715132953966	2.8828481107598076
4	1.8215974424438608	1.9448121162052714
5	1.0919688944177952	1.4572884538734
6	0.7795924122936422	1.2484872347231817
7	0.5975296413181721	1.1164075022907118
8	0.4832161791721966	1.031802810109037
9	0.4337392004691562	1.0419249465080962
>10	5.999612774629765	37.299463442749726
>50	0.6659164732938766	11.554029051530565
>100	0.1363082735487762	6.204808674911876
>500	0.006823595014404423	1.2575767749746882
>1k	0.00432715781401256	2.150896059493974
>5k	4.992874400783723E-4	0.7517475013386312
>10k+	4.992874400783723E-4	4.8292103121802645
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	80807	3.549768450387937	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	17787	0.781364627161635	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG	10119	0.4445172689182315	No Hit
CCTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGCCGTCTTCTGC	5860	0.25742377664401983	No Hit
CTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGCCGTCTTCTGCT	5794	0.25452446448386534	No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	5269	0.23146175411900005	No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	4646	0.20409400448602663	No Hit
GCTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGCCGTCTTCTGC	4363	0.19166210537506118	No Hit
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	3402	0.14944636316432686	No Hit
CGCTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGCCGTCTTCTG	3243	0.14246165659668195	No Hit
TGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	2757	0.12111217614463526	No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA	2322	0.10200307327088975	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.2644524121837883	0.0
2	0.0	0.0	0.0	0.8543745788309798	0.0
3	0.0	0.0	0.0	1.436345601523808	0.0
4	0.0	0.0	0.0	1.826303087064587	0.0
5	0.0	0.0	0.0	3.8336374682503354	0.0
6	0.0	0.0	0.0	4.485235911758995	0.0
7	0.0	0.0	0.0	6.522222349128142	0.0
8	0.0	0.0	0.0	8.809955359378527	0.0
9	0.0	0.0	0.0	10.908530215664896	0.0
10	0.0	0.0	0.0	12.07664551340229	0.0
11	0.0	0.0	0.0	13.050287251548715	0.0
12	0.0	0.0	0.0	13.7672080766051	0.0
13	0.0	0.0	0.0	14.129182806903174	0.0
14	0.0	0.0	0.0	14.355812374088584	0.0
15	0.0	0.0	0.0	15.009475479287051	0.0
16	0.0	0.0	0.0	15.731755638942508	0.0
17	4.392897212355287E-5	0.0	0.0	16.640909646011558	0.0
18	4.392897212355287E-5	0.0	0.0	17.150793225449636	0.0
19	4.392897212355287E-5	0.0	0.0	17.703858984485166	0.0
20	4.392897212355287E-5	0.0	0.0	18.307970209128264	0.0
21	4.392897212355287E-5	0.0	0.0	18.93009231234202	0.0
22	8.785794424710574E-5	0.0	0.0	19.5324903070723	0.0
23	8.785794424710574E-5	0.0	0.0	20.076550626822502	0.0
24	8.785794424710574E-5	0.0	0.0	20.51865180227394	0.0
25	8.785794424710574E-5	0.0	0.0	20.94612463000823	0.0
26	8.785794424710574E-5	0.0	0.0	21.36973170819565	0.0
27	8.785794424710574E-5	0.0	0.0	21.815962207026704	0.0
28	8.785794424710574E-5	0.0	0.0	22.268869909620534	0.0
29	8.785794424710574E-5	0.0	0.0	22.734165582353207	0.0
30	8.785794424710574E-5	0.0	0.0	23.169238122264872	0.0
31	8.785794424710574E-5	0.0	0.0	23.617840785590595	0.0
32	8.785794424710574E-5	0.0	0.0	24.093152263967436	0.0
33	8.785794424710574E-5	0.0	0.0	24.539250975882116	0.0
34	8.785794424710574E-5	0.0	0.0	25.033979059937568	0.0
35	8.785794424710574E-5	0.0	0.0	25.455345760546688	0.0
36	8.785794424710574E-5	0.0	0.0	25.904914861259126	0.0
37	8.785794424710574E-5	0.0	0.0	26.42696676597543	0.0
38	8.785794424710574E-5	0.0	0.0	26.99597874189181	0.0
39	8.785794424710574E-5	0.0	0.0	27.6823250023502	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTTTA	40	6.8193913E-9	45.0	37
ACACGAA	20	7.0347456E-4	45.0	27
CGTTTTT	20465	0.0	44.0215	1
GGCGATA	485	0.0	41.288662	7
CTTACTG	4745	0.0	41.15911	38
ACCCGCA	4850	0.0	40.917526	31
GCACTTA	4805	0.0	40.87929	35
CCCGCAC	4915	0.0	40.788403	32
CACTTAC	4800	0.0	40.78125	36
CGCACTT	4850	0.0	40.77835	34
CCGCACT	4860	0.0	40.648148	33
CGGTCTA	355	0.0	40.563377	30
ACAACGG	100	0.0	40.5	1
ATTACGG	200	0.0	40.5	1
ACTTACT	4835	0.0	40.25336	37
ACGGGAT	425	0.0	40.235294	4
CACGACC	600	0.0	40.125	26
AATACGG	135	0.0	40.000004	1
TCATCGG	90	0.0	40.0	1
TTATGAC	5320	0.0	39.92481	26
>>END_MODULE