FastQCFastQC Report
Thu 26 May 2016
SRR1043360_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1043360_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1033644
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT754857.302804447179106No Hit
CCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCTGCT55280.5348069548122951No Hit
CTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCTGCTT54730.5294859738943001Illumina Single End Adapter 1 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCTGC38150.3690825854936516No Hit
GCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCTGCT37970.36734117355685325No Hit
CGTTCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCT26770.2589866530449555No Hit
CGTTTCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTC24550.23750923915777578No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTT19740.19097484240221974No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCG19640.19000739132622063No Hit
TCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCTGCT18390.17791425287623205No Hit
CGTCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCTG16330.15798476071065087No Hit
GCCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCTGC15250.14753628908986072No Hit
ACCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCTGC14320.13853899408306922No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCT13900.13447569956387306No Hit
AGCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCTGC11480.11106338352469516No Hit
TCCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCTGC10830.1047749515307011No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGATG351.0194344E-746.00000446
CATCGCG351.0194344E-746.00000418
ACATCGC351.0194344E-746.00000417
TTCGCGA351.0194344E-746.00000444
TCGCGAT351.0194344E-746.00000445
ATCGATC351.0194344E-746.00000442
TCACGAC1850.046.024
AGATCGT253.416915E-546.042
CTCCGTG206.3111575E-446.034
GATCGCG206.3111575E-446.08
CGACGGT1800.046.027
TAGACGG1050.046.01
TAGCGGA206.3111575E-446.02
ATTGTCG206.3111575E-446.021
CTAAGCA206.3111575E-446.018
TCGGCTA253.416915E-546.037
TATTGGT301.8612209E-646.022
GCCGACA405.6097633E-946.08
AATTGCG253.416915E-546.026
AGACCCG206.3111575E-446.038
Produced by FastQC (version 0.11.5)