##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043359_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1001177
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.12597872304298	33.0	31.0	34.0	30.0	34.0
2	32.45337437835668	34.0	31.0	34.0	31.0	34.0
3	32.51188551075384	34.0	31.0	34.0	31.0	34.0
4	36.107446535427805	37.0	35.0	37.0	35.0	37.0
5	36.06004532665053	37.0	35.0	37.0	35.0	37.0
6	36.2654056175881	37.0	37.0	37.0	35.0	37.0
7	36.197343726433985	37.0	37.0	37.0	35.0	37.0
8	36.103557113277674	37.0	37.0	37.0	35.0	37.0
9	37.67915763146776	39.0	38.0	39.0	35.0	39.0
10	37.50348439886254	39.0	37.0	39.0	35.0	39.0
11	37.24669563923263	39.0	37.0	39.0	34.0	39.0
12	37.267404265179884	39.0	37.0	39.0	34.0	39.0
13	37.27986060406901	39.0	37.0	39.0	34.0	39.0
14	38.685532128684535	40.0	38.0	41.0	35.0	41.0
15	38.793417148016786	40.0	38.0	41.0	35.0	41.0
16	38.838285338156986	40.0	38.0	41.0	35.0	41.0
17	38.79658142366435	40.0	38.0	41.0	35.0	41.0
18	38.50818886170977	40.0	38.0	41.0	35.0	41.0
19	38.201739552546655	40.0	37.0	41.0	35.0	41.0
20	37.893169739216944	40.0	35.0	41.0	34.0	41.0
21	37.84856823518719	40.0	35.0	41.0	34.0	41.0
22	37.80473782358165	40.0	35.0	41.0	34.0	41.0
23	37.77566304459651	39.0	35.0	41.0	34.0	41.0
24	37.68110833548913	39.0	35.0	41.0	34.0	41.0
25	37.65832914659446	39.0	35.0	41.0	34.0	41.0
26	37.626946084458595	39.0	35.0	41.0	34.0	41.0
27	37.60998604642336	39.0	35.0	41.0	34.0	41.0
28	37.524235974258296	39.0	35.0	41.0	34.0	41.0
29	37.50521835799264	39.0	35.0	41.0	34.0	41.0
30	37.408197551481905	39.0	35.0	41.0	33.0	41.0
31	37.199498190629626	39.0	35.0	41.0	33.0	41.0
32	36.943812133119316	39.0	35.0	41.0	33.0	41.0
33	36.557049352911626	39.0	35.0	41.0	31.0	41.0
34	36.196687498813894	39.0	35.0	41.0	30.0	41.0
35	35.936870303652604	39.0	35.0	41.0	29.0	41.0
36	35.87358279305258	39.0	35.0	41.0	29.0	41.0
37	35.6930472833475	39.0	35.0	41.0	27.0	41.0
38	35.63916070784686	39.0	35.0	41.0	27.0	41.0
39	35.544911639000894	39.0	35.0	41.0	26.0	41.0
40	35.48620473702452	39.0	35.0	41.0	26.0	41.0
41	35.418402540210174	38.0	35.0	40.0	25.0	41.0
42	35.34844288272703	38.0	35.0	40.0	25.0	41.0
43	35.220900000699174	38.0	35.0	40.0	24.0	41.0
44	35.131885770448186	38.0	35.0	40.0	24.0	41.0
45	35.09674313333207	38.0	35.0	40.0	23.0	41.0
46	35.01989458407454	38.0	35.0	40.0	23.0	41.0
47	34.858209886963046	38.0	35.0	40.0	23.0	41.0
48	34.82477823601621	38.0	35.0	40.0	23.0	41.0
49	34.717092981560704	38.0	35.0	40.0	23.0	41.0
50	34.60862764526153	38.0	35.0	40.0	23.0	41.0
51	34.139200161410024	37.0	34.0	40.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	0.0
14	3.0
15	8.0
16	34.0
17	65.0
18	168.0
19	373.0
20	705.0
21	1140.0
22	1826.0
23	3091.0
24	5545.0
25	10934.0
26	17592.0
27	20022.0
28	18957.0
29	17122.0
30	17369.0
31	19643.0
32	23375.0
33	30428.0
34	54092.0
35	91248.0
36	99833.0
37	77463.0
38	136235.0
39	353850.0
40	55.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.87154918660736	18.988051063897792	25.32329448239422	29.817105267100626
2	35.672513451667385	18.650348539768693	25.062301670933312	20.61483633763061
3	20.637609533578978	20.937756260880942	39.225531549366394	19.199102656173682
4	21.8893362512323	23.566861803657098	36.985967516233394	17.55783442887721
5	19.83135849105603	29.012252578714854	33.19123391767889	17.96515501255023
6	71.78950375408144	1.5126196466758624	22.941597739460654	3.7562788597820362
7	72.17524973106654	1.0952109367274718	19.908267968600956	6.821271363605037
8	60.58998558696415	3.040221659107231	27.56525569404811	8.804537059880522
9	34.02255545223272	17.342787539066517	30.82651718926823	17.80813981943253
10	26.105773504585105	19.598033115023618	42.50137588058855	11.79481749980273
11	23.176221587191876	16.051707140695402	41.83775696005801	18.934314312054713
12	19.328750061177992	17.911118613392038	44.29586376834466	18.46426755708531
13	13.730239508098968	21.32440117981136	43.179377872244366	21.7659814398453
14	12.66938813017079	21.296733744382863	45.20739090090963	20.82648722453672
15	13.725345268618835	17.954567474083007	47.87704871366402	20.44303854363414
16	14.393558781314391	23.63328362517317	41.12779258812378	20.845365005388658
17	15.97150154268426	21.129730307428158	42.94844967473284	19.950318475154745
18	17.66001416332976	27.225255873836495	39.5465537062877	15.568176256546046
19	16.82050226882959	23.26022271786108	37.73678380546097	22.18249120784836
20	14.35969863470695	28.783322029970726	36.2188703895515	20.638108945770828
21	14.745244846815297	23.357308447956754	37.77613748617877	24.121309219049177
22	13.14013406220878	26.2301271403558	34.664000471445114	25.965738325990312
23	12.486603267953617	22.75092216461225	43.22202767342838	21.540446894005754
24	16.654897186012064	21.612062602317074	40.445096121864566	21.2879440898063
25	13.606185519643379	24.71790702343342	40.125572201518814	21.55033525540439
26	16.25217119450407	22.108977733208015	38.35235927313552	23.286491799152397
27	20.660083082212235	22.924417960061007	38.61015584656859	17.805343111158166
28	16.77036128476783	22.885863338850175	35.46106232963802	24.88271304674398
29	17.538657000710163	27.179709481939756	33.23877795834303	22.042855559007048
30	23.745451603462726	23.354012327490544	30.531264701446396	22.369271367600334
31	19.889490070187392	24.598947039334703	30.498703026537765	25.01285986394014
32	19.60033041110613	28.783621677285836	29.760172277229703	21.855875634378336
33	19.00932602327061	27.13306438322095	30.602680644880977	23.254928948627466
34	22.097291487918717	24.59205515108717	30.227322441486372	23.083330919507738
35	18.619384983873978	28.323363401276698	28.907975313056532	24.149276301792792
36	23.73256676891299	28.38888628084744	28.51943262779708	19.359114322442487
37	20.756369752800953	25.894022735240625	32.17293245849635	21.176675053462073
38	21.99171575056159	23.147854974694784	29.44943801146051	25.410991263283115
39	25.408494202323865	21.799042526945787	31.710676533719813	21.08178673701054
40	20.88312056709253	19.569067207896307	36.50453416328981	23.043278061721352
41	21.776868625627635	22.20286722527585	31.126464151693455	24.893799997403057
42	21.45075246434946	19.015618616887924	35.75901164329584	23.774617275466774
43	22.85020530835207	23.13656825915897	33.4955757073924	20.517650725096562
44	17.515783922323426	25.812119135777188	34.65740823051269	22.014688711386697
45	20.24856743612768	23.103107642305005	33.82109257404036	22.82723234752696
46	24.12740204778975	20.003655697244344	32.40036477066493	23.46857748430098
47	19.29049508728227	18.96028374603092	35.672113921913905	26.0771072447729
48	20.41147569310921	17.515284510131576	31.89226280667654	30.180976990082677
49	20.735294558304876	18.054549794891415	36.61180790209923	24.598347744704483
50	19.686628837857842	17.327805173311013	37.85963920465612	25.12592678417502
51	17.561130549343424	16.41328156759494	37.5303268053501	28.495261077711532
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	150.0
1	1030.0
2	1910.0
3	39609.0
4	77308.0
5	43431.0
6	9554.0
7	9032.5
8	8511.0
9	8753.5
10	8996.0
11	9020.5
12	9045.0
13	8717.5
14	8390.0
15	7845.0
16	7300.0
17	6623.5
18	5947.0
19	5470.0
20	4993.0
21	4627.0
22	4261.0
23	3975.0
24	3689.0
25	3671.5
26	3774.5
27	3895.0
28	4103.5
29	4312.0
30	5376.0
31	6440.0
32	7564.0
33	8688.0
34	9910.0
35	11132.0
36	12757.0
37	14382.0
38	16876.0
39	19370.0
40	22960.5
41	26551.0
42	31834.5
43	37118.0
44	42364.0
45	47610.0
46	55675.5
47	63741.0
48	85176.5
49	106612.0
50	130158.5
51	153705.0
52	149483.5
53	145262.0
54	116873.0
55	88484.0
56	67251.5
57	46019.0
58	35707.5
59	25396.0
60	19339.5
61	13283.0
62	10958.5
63	8634.0
64	6999.0
65	5364.0
66	4396.0
67	3428.0
68	2948.0
69	2468.0
70	2084.0
71	1700.0
72	1600.0
73	1500.0
74	1273.5
75	835.0
76	623.0
77	480.5
78	338.0
79	270.5
80	203.0
81	148.5
82	94.0
83	66.0
84	38.0
85	26.5
86	15.0
87	11.0
88	7.0
89	4.5
90	2.0
91	2.0
92	2.0
93	2.5
94	3.0
95	2.0
96	1.0
97	1.0
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1001177.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.320567059762254
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.18912993733247	26.91043273176491
2	11.139196620126906	7.868854824261389
3	3.9777170409218154	4.214856644659141
4	1.9913752827956641	2.813460168685491
5	1.1550609368979556	2.039870363990803
6	0.7613955611016949	1.6135753784938633
7	0.5687639968508442	1.4062346824364058
8	0.43681232107082624	1.2342767103130015
9	0.3514836721863145	1.117314235248139
>10	2.9424390942092336	21.50863322708256
>50	0.32603076221826993	7.84719377751394
>100	0.14948981649168264	9.289092793710028
>500	0.006549077674821801	1.5366497996408326
>1k	0.003701652598812323	2.6272598151722932
>5k	5.694850152018958E-4	1.0718029265509754
>10k+	2.847425076009479E-4	6.900491920476261
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	68612	6.853133861445079	No Hit
CCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCTGC	5336	0.5329726911425253	No Hit
CTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCTGCT	5321	0.5314744545669747	No Hit
GCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCTGC	3967	0.39623363301394254	No Hit
CGCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCTG	3652	0.3647706649273805	No Hit
CGTTCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTC	2548	0.254500452966858	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTT	2348	0.23452396529285033	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCT	1949	0.19467087238320496	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCC	1882	0.1879787490124124	No Hit
TCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCTGC	1864	0.1861808651217517	No Hit
GCCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCTG	1480	0.14782600878765692	No Hit
CGTCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCT	1479	0.1477261263492869	No Hit
ACCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCTG	1427	0.14253223955404487	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTC	1274	0.127250226483429	No Hit
AGCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCTG	1197	0.11955927872893603	No Hit
TCCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCTG	1056	0.10547585491876063	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.5441595242399696	0.0
2	0.0	0.0	0.0	1.7856982331795477	0.0
3	0.0	0.0	0.0	3.103746889910575	0.0
4	0.0	0.0	0.0	3.9617370355092056	0.0
5	0.0	0.0	0.0	8.324801708389225	0.0
6	0.0	0.0	0.0	9.866686909507509	0.0
7	0.0	0.0	0.0	14.167225175967886	0.0
8	0.0	0.0	0.0	19.237857042261258	0.0
9	0.0	0.0	0.0	23.514823053266305	0.0
10	0.0	0.0	0.0	25.767072156072302	0.0
11	0.0	0.0	0.0	27.660943070006603	0.0
12	0.0	0.0	0.0	28.97849231454578	0.0
13	0.0	0.0	0.0	29.567698818490637	0.0
14	0.0	0.0	0.0	30.008180371702505	0.0
15	0.0	0.0	0.0	31.26510097615107	0.0
16	0.0	0.0	0.0	32.71469480421544	0.0
17	0.0	0.0	0.0	34.328994773152	0.0
18	0.0	0.0	0.0	35.265092985556	0.0
19	0.0	0.0	0.0	36.28669056520475	0.0
20	0.0	0.0	0.0	37.374709966369586	0.0
21	0.0	0.0	0.0	38.471818669426085	0.0
22	9.988243837003846E-5	0.0	0.0	39.51628932746158	0.0
23	9.988243837003846E-5	0.0	0.0	40.373979825745096	0.0
24	4.994121918501923E-4	0.0	0.0	41.123098113520385	0.0
25	4.994121918501923E-4	0.0	0.0	41.80799199342374	0.0
26	4.994121918501923E-4	0.0	0.0	42.530241905277485	0.0
27	4.994121918501923E-4	0.0	0.0	43.2189313178389	0.0
28	4.994121918501923E-4	0.0	0.0	43.90831990746891	0.0
29	4.994121918501923E-4	0.0	0.0	44.7274557845416	0.0
30	4.994121918501923E-4	0.0	0.0	45.432326152118954	0.0
31	4.994121918501923E-4	0.0	0.0	46.05788986363051	0.0
32	4.994121918501923E-4	0.0	0.0	46.6826545156351	0.0
33	4.994121918501923E-4	0.0	0.0	47.293635391144626	0.0
34	5.992946302202308E-4	0.0	0.0	47.95086183561948	0.0
35	5.992946302202308E-4	0.0	0.0	48.548158817072306	0.0
36	5.992946302202308E-4	0.0	0.0	49.09281775350413	0.0
37	5.992946302202308E-4	0.0	0.0	49.66084918051453	0.0
38	5.992946302202308E-4	0.0	0.0	50.210502238864855	0.0
39	5.992946302202308E-4	0.0	0.0	50.76235271085932	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGAC	130	0.0	45.000004	24
TTCGATT	85	0.0	45.000004	39
TAGTCGG	30	2.1652195E-6	45.000004	1
TACACGG	60	0.0	45.000004	1
TCGCAGT	25	3.8904447E-5	45.0	40
CATGCGG	145	0.0	45.0	1
AACTTCG	110	0.0	45.0	10
CGCGCAG	50	2.1827873E-11	45.0	34
GTTAGTA	20	7.03285E-4	45.0	29
CTAAGCA	25	3.8904447E-5	45.0	18
TACGGAA	20	7.03285E-4	45.0	3
AGACGGT	25	3.8904447E-5	45.0	2
CCATCGA	25	3.8904447E-5	45.0	40
ACATACG	40	6.8139343E-9	45.0	16
GCCGTGG	20	7.03285E-4	45.0	13
GCCCGAC	20	7.03285E-4	45.0	8
ATCGATC	25	3.8904447E-5	45.0	42
GTACGAC	20	7.03285E-4	45.0	8
ACGGTAG	20	7.03285E-4	45.0	4
CGTTTTT	14670	0.0	44.616566	1
>>END_MODULE