FastQCFastQC Report
Thu 26 May 2016
SRR1043358_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1043358_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1287534
Sequences flagged as poor quality0
Sequence length52
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT536314.165404564073648No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC82150.6380414031784792No Hit
CCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCTGCT57770.4486871802997047No Hit
CTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCTGCTT48750.37863077790567085Illumina Single End Adapter 2 (95% over 21bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA41910.3255059672210598No Hit
GCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCTGCT39070.3034482972876833No Hit
CGCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCTGC21570.1675295565010322No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCG18320.14228750464065415No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC17740.13778276923172514No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC14530.11285138877885945No Hit
TCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCTGCT14060.10920099974058936No Hit
GCCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCTGC13140.10205555736780543No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTAGCG351.0197073E-746.00000410
CGCATTA206.311738E-446.036
ATTACGT253.417387E-546.011
CCGGTTA206.311738E-446.026
CCAACGA206.311738E-446.045
GTACGAT206.311738E-446.026
AAGTCCG206.311738E-446.010
CGTTTTT127750.045.225831
CACGACG3450.044.66666825
CGGTCTA3550.044.0563430
TCACGAC3700.043.5135124
TACGGGA1850.043.513513
ATTGCGG1800.043.4444471
AATGCGG1600.043.1251
GCGATAT700.042.7142878
CTTGCGG3450.042.6666681
ACCCGCA19300.042.66320831
CACTTAC18900.042.59259436
CCGCACT19050.042.49868833
CGCACTT19100.042.38743234