##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043358_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1287534
Sequences flagged as poor quality	0
Sequence length	52
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.35035424307242	33.0	31.0	34.0	31.0	34.0
2	32.68159520447615	34.0	31.0	34.0	31.0	34.0
3	32.82143694846117	34.0	31.0	34.0	31.0	34.0
4	36.25278555750761	37.0	37.0	37.0	35.0	37.0
5	36.18483317722095	37.0	37.0	37.0	35.0	37.0
6	36.33155551620384	37.0	37.0	37.0	35.0	37.0
7	36.2617220205447	37.0	37.0	37.0	35.0	37.0
8	36.25535636340477	37.0	37.0	37.0	35.0	37.0
9	37.78007027387238	39.0	38.0	39.0	35.0	39.0
10	37.62277734024888	39.0	37.0	39.0	35.0	39.0
11	37.33468630731305	39.0	37.0	39.0	35.0	39.0
12	37.34554349632709	39.0	37.0	39.0	35.0	39.0
13	37.343690341381276	39.0	37.0	39.0	35.0	39.0
14	38.69785885265942	40.0	38.0	41.0	35.0	41.0
15	38.760257981536796	41.0	38.0	41.0	35.0	41.0
16	38.644212114010195	40.0	38.0	41.0	34.0	41.0
17	38.63906584214475	40.0	38.0	41.0	34.0	41.0
18	38.453713843673256	40.0	37.0	41.0	34.0	41.0
19	38.22100309584057	40.0	37.0	41.0	34.0	41.0
20	37.93410193439552	40.0	35.0	41.0	34.0	41.0
21	37.87039487889252	40.0	35.0	41.0	34.0	41.0
22	37.831797063223185	40.0	35.0	41.0	34.0	41.0
23	37.81082984993018	40.0	35.0	41.0	34.0	41.0
24	37.72544259025393	40.0	35.0	41.0	34.0	41.0
25	37.600815201773315	40.0	35.0	41.0	34.0	41.0
26	37.51207269089593	39.0	35.0	41.0	34.0	41.0
27	37.53960905110079	39.0	35.0	41.0	34.0	41.0
28	37.48146301379226	39.0	35.0	41.0	33.0	41.0
29	37.42323930863185	39.0	35.0	41.0	33.0	41.0
30	37.36895336356166	39.0	35.0	41.0	33.0	41.0
31	37.28493150472143	40.0	35.0	41.0	33.0	41.0
32	37.03589963449509	39.0	35.0	41.0	33.0	41.0
33	36.75565460795598	39.0	35.0	41.0	32.0	41.0
34	36.561415853872596	39.0	35.0	41.0	31.0	41.0
35	36.397605034119486	39.0	35.0	41.0	31.0	41.0
36	36.33585831519789	39.0	35.0	41.0	31.0	41.0
37	36.28519945881041	39.0	35.0	41.0	31.0	41.0
38	36.17783918715933	39.0	35.0	41.0	31.0	41.0
39	36.12340334313502	39.0	35.0	41.0	31.0	41.0
40	36.02709520680619	39.0	35.0	41.0	31.0	41.0
41	35.97393699894527	38.0	35.0	41.0	30.0	41.0
42	35.865555395041994	38.0	35.0	41.0	30.0	41.0
43	35.753716018373105	38.0	35.0	41.0	30.0	41.0
44	35.57478637457341	38.0	35.0	40.0	29.0	41.0
45	35.45556932865462	37.0	35.0	40.0	29.0	41.0
46	35.38176001565784	37.0	35.0	40.0	29.0	41.0
47	35.278627981863	37.0	35.0	40.0	28.0	41.0
48	35.162655898795684	37.0	35.0	40.0	28.0	41.0
49	35.082834317385014	36.0	35.0	40.0	28.0	41.0
50	34.88478595516701	36.0	35.0	40.0	28.0	41.0
51	34.681396374775346	36.0	35.0	39.0	27.0	41.0
52	34.29861580354383	35.0	34.0	39.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	4.0
13	1.0
14	7.0
15	28.0
16	49.0
17	115.0
18	251.0
19	495.0
20	914.0
21	1523.0
22	2386.0
23	3927.0
24	6642.0
25	11603.0
26	16482.0
27	19546.0
28	20018.0
29	19680.0
30	20802.0
31	23795.0
32	28987.0
33	37474.0
34	81010.0
35	130921.0
36	115423.0
37	119104.0
38	187950.0
39	437462.0
40	934.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.287406779160783	17.21849675426047	22.545657046726532	28.948439419852214
2	34.34084070789587	19.633034933446417	24.759035489548236	21.267088869109475
3	26.096475898888883	20.929000709884164	33.001303266554515	19.973220124672437
4	23.81405073574756	23.87338897458242	30.876621510577586	21.435938779092435
5	21.596788900331955	31.754112901096203	28.71574653562547	17.933351662946375
6	78.5749347201705	1.9194832913150255	16.632725815395943	2.872856173118535
7	78.8492575730039	2.103711435969846	14.826559919971045	4.220471071055211
8	69.7308964268128	3.5444500883083476	20.208165376603645	6.516488108275199
9	39.115005894989956	16.972833338770084	25.91558747186482	17.99657329437514
10	27.531544798040287	17.743143093696943	35.09243251052011	19.63287959774266
11	24.447432067813356	15.195326880688198	38.55051594754003	21.806725103958417
12	20.055315044107573	18.032300506239057	40.72506046442268	21.18732398523068
13	16.10559410469937	21.411162734343325	42.19515756477111	20.288085596186196
14	16.28337581764831	19.83023360936488	41.87524368288527	22.011146890101543
15	19.3747116580999	16.071342582021135	42.671649836043166	21.882295923835798
16	17.99051520192865	19.87396060997224	37.92847412184843	24.207050066250677
17	19.23071546071793	19.935551216511563	37.46899111013767	23.36474221263283
18	20.702909593067055	24.97363176428739	34.27202699113188	20.051431651513667
19	22.14729863444383	21.022668139249138	33.429020126847135	23.401013099459895
20	20.43130511504939	25.11778329737312	35.16885767676815	19.282053910809346
21	19.438787635899324	19.9024647116115	35.75439561207704	24.904352040412135
22	19.458126931016967	21.57333320906477	32.7305531349075	26.23798672501076
23	19.762973249638456	20.20568000534355	38.59781566933378	21.433531075684215
24	23.49631155371431	18.62063448421556	34.024421879344544	23.858632082725585
25	18.012417536158267	21.90404292236166	34.60390172220695	25.479637819273123
26	17.95463265436097	20.25996983380633	34.9432325670623	26.842164944770392
27	21.817520935369476	20.966048275230012	34.82766280346771	22.388767985932798
28	21.490383943258976	21.262739469404305	32.87385032162257	24.37302626571415
29	23.155039012562	19.680179319536418	34.257425434978806	22.907356232922783
30	23.955095554758167	20.28148382877656	31.079179268275638	24.68424134818964
31	27.50940946025503	19.501931599476208	30.1233986830639	22.86526025720486
32	24.700862268491548	20.20327230193533	30.673908417175777	24.421957012397343
33	20.770635959904748	21.05831768326118	32.30982638128391	25.86121997555016
34	21.7401637548989	20.440858260830392	34.69438476964492	23.12459321462579
35	23.152320637746264	24.033928424414423	30.204173249017114	22.6095776888222
36	21.900392533323394	23.91641696452288	31.072810504421632	23.1103799977321
37	21.335669582317827	25.34729179967286	32.43525996206702	20.88177865594229
38	19.705654374952427	24.902022004855795	29.911054776029218	25.481268844162564
39	22.614781434898028	22.426669897649305	33.16821148024052	21.790337187212145
40	18.72300071299088	21.49784005703927	34.99767773122884	24.781481498741005
41	21.19268306701027	24.74552128332145	28.681883352206622	25.379912297461658
42	20.494915085737542	19.852368947150133	32.554169443292366	27.098546523819955
43	22.97112153931469	19.862931775005556	32.882704456736676	24.28324222894308
44	21.63142876226958	20.958669829301595	33.228559401149795	24.18134200727903
45	22.018525336029963	21.19322674197342	32.21848898747528	24.569758934521342
46	22.247257159810925	21.924624903109354	31.877837789138	23.950280147941726
47	19.241433624277107	22.734545262494038	34.76413050063144	23.259890612597413
48	21.393609799818876	20.764733203162013	31.126323654365635	26.715333342653473
49	21.117578254244158	19.029633392205564	34.890962102748354	24.96182625080192
50	19.90774612553921	19.455020216941843	35.73692034540447	24.900313312114474
51	19.230171785754784	18.10290058359624	34.40157696806453	28.265350662584442
52	18.023291035421202	19.35692572001982	38.197049553642856	24.422733690916125
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	473.0
1	2443.0
2	4413.0
3	36179.0
4	67945.0
5	38537.0
6	9129.0
7	8473.0
8	7817.0
9	7714.0
10	7611.0
11	7634.5
12	7658.0
13	7301.0
14	6596.0
15	6248.0
16	5889.5
17	5531.0
18	5226.0
19	4921.0
20	4828.0
21	4735.0
22	4577.5
23	4420.0
24	4601.0
25	4782.0
26	5508.5
27	6235.0
28	6953.5
29	7672.0
30	8915.5
31	10159.0
32	11415.5
33	12672.0
34	14689.5
35	16707.0
36	18413.5
37	20120.0
38	22329.5
39	26545.0
40	28551.0
41	33785.5
42	39020.0
43	47298.5
44	55577.0
45	67509.0
46	79441.0
47	95496.5
48	111552.0
49	121063.5
50	130575.0
51	120708.5
52	110842.0
53	97468.5
54	84095.0
55	76641.5
56	69188.0
57	64994.0
58	60800.0
59	58639.0
60	56478.0
61	54162.0
62	51846.0
63	50981.0
64	43397.5
65	36679.0
66	30310.0
67	23941.0
68	20008.5
69	16076.0
70	14145.5
71	12215.0
72	12724.5
73	13234.0
74	10629.0
75	8024.0
76	6041.0
77	4058.0
78	3084.5
79	2111.0
80	1633.0
81	1155.0
82	917.5
83	680.0
84	509.5
85	339.0
86	224.0
87	109.0
88	83.0
89	42.0
90	27.0
91	16.0
92	5.0
93	3.5
94	2.0
95	5.5
96	9.0
97	5.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1287534.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.136888293947464
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.58063887523205	21.73047743798872
2	10.655393519427793	6.209300214072387
3	3.9421068267801083	3.4458217876409925
4	1.8319475086278747	2.13508999677063
5	1.0753862103939757	1.566670394255038
6	0.7240922584779028	1.2658677149869675
7	0.5191060274677467	1.0587594034529793
8	0.4362210333436028	1.0168098816002311
9	0.35585584527159664	0.9331678811184088
>10	5.226207538922653	36.388272363051584
>50	0.5392865045382298	10.010253685618423
>100	0.10248938509736752	5.3763717759605045
>500	0.006707420487585287	1.3580273423030003
>1k	0.0037561554730477613	2.2187940396376957
>5k	5.36593639006823E-4	1.0938014375721608
>10k+	2.682968195034115E-4	4.192514643970308
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	53631	4.165404564073648	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	8215	0.6380414031784792	No Hit
CCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCTGCT	5777	0.4486871802997047	No Hit
CTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCTGCTT	4875	0.37863077790567085	Illumina Single End Adapter 2 (95% over 21bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA	4191	0.3255059672210598	No Hit
GCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCTGCT	3907	0.3034482972876833	No Hit
CGCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCTGC	2157	0.1675295565010322	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCG	1832	0.14228750464065415	No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	1774	0.13778276923172514	No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	1453	0.11285138877885945	No Hit
TCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCTGCT	1406	0.10920099974058936	No Hit
GCCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCTGC	1314	0.10205555736780543	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.3912129699099208	0.0
2	0.0	0.0	0.0	1.3410907983789166	0.0
3	0.0	0.0	0.0	2.1108568783426302	0.0
4	0.0	0.0	0.0	2.6054457591022837	0.0
5	0.0	0.0	0.0	5.19295024442073	0.0
6	0.0	0.0	0.0	5.750294749497877	0.0
7	0.0	0.0	0.0	7.641584610581157	0.0
8	0.0	0.0	0.0	9.854730049847227	0.0
9	0.0	0.0	0.0	11.598373324510264	0.0
10	0.0	0.0	0.0	12.843233654412233	0.0
11	0.0	0.0	0.0	13.985727755538884	0.0
12	0.0	0.0	0.0	14.849471936275082	0.0
13	0.0	0.0	0.0	15.256839819375644	0.0
14	7.766785187808633E-5	0.0	0.0	15.534813061247315	0.0
15	7.766785187808633E-5	0.0	0.0	16.44896367785239	0.0
16	7.766785187808633E-5	0.0	0.0	17.42439422958928	0.0
17	1.5533570375617266E-4	0.0	0.0	18.568752359161	0.0
18	1.5533570375617266E-4	0.0	0.0	19.224113693308293	0.0
19	1.5533570375617266E-4	0.0	0.0	19.88778548760654	0.0
20	1.5533570375617266E-4	0.0	0.0	20.669357081055722	0.0
21	1.5533570375617266E-4	0.0	0.0	21.401609588562323	0.0
22	2.3300355563425897E-4	0.0	0.0	22.119415875619595	0.0
23	2.3300355563425897E-4	0.0	0.0	22.7970678832559	0.0
24	4.6600711126851794E-4	0.0	0.0	23.30641365587239	0.0
25	9.320142225370359E-4	0.0	0.0	23.804031582855288	0.0
26	9.320142225370359E-4	0.0	0.0	24.270116361975685	0.0
27	9.320142225370359E-4	0.0	0.0	24.793675351485863	0.0
28	9.320142225370359E-4	0.0	0.0	25.328496179518368	0.0
29	9.320142225370359E-4	0.0	0.0	25.86479269673655	0.0
30	9.320142225370359E-4	0.0	0.0	26.3668376912765	0.0
31	9.320142225370359E-4	0.0	0.0	26.848067701513123	0.0
32	9.320142225370359E-4	0.0	0.0	27.377684783469796	0.0
33	9.320142225370359E-4	0.0	0.0	27.86264285059657	0.0
34	9.320142225370359E-4	0.0	0.0	28.39987138203729	0.0
35	9.320142225370359E-4	0.0	0.0	28.854927326190996	0.0
36	9.320142225370359E-4	0.0	0.0	29.32613818353535	0.0
37	9.320142225370359E-4	0.0	0.0	29.867250107569976	0.0
38	9.320142225370359E-4	0.0	0.0	30.425448959017782	0.0
39	9.320142225370359E-4	0.0	0.0	31.053626545007745	0.0
40	9.320142225370359E-4	0.0	0.0	31.655940736322304	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTAGCG	35	1.0197073E-7	46.000004	10
CGCATTA	20	6.311738E-4	46.0	36
ATTACGT	25	3.417387E-5	46.0	11
CCGGTTA	20	6.311738E-4	46.0	26
CCAACGA	20	6.311738E-4	46.0	45
GTACGAT	20	6.311738E-4	46.0	26
AAGTCCG	20	6.311738E-4	46.0	10
CGTTTTT	12775	0.0	45.22583	1
CACGACG	345	0.0	44.666668	25
CGGTCTA	355	0.0	44.05634	30
TCACGAC	370	0.0	43.51351	24
TACGGGA	185	0.0	43.51351	3
ATTGCGG	180	0.0	43.444447	1
AATGCGG	160	0.0	43.125	1
GCGATAT	70	0.0	42.714287	8
CTTGCGG	345	0.0	42.666668	1
ACCCGCA	1930	0.0	42.663208	31
CACTTAC	1890	0.0	42.592594	36
CCGCACT	1905	0.0	42.498688	33
CGCACTT	1910	0.0	42.387432	34
>>END_MODULE