##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043357_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1260208
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.33778868250321	33.0	31.0	34.0	31.0	34.0
2	32.62441358886787	34.0	31.0	34.0	31.0	34.0
3	32.73638796135241	34.0	31.0	34.0	31.0	34.0
4	36.2221299975877	37.0	37.0	37.0	35.0	37.0
5	36.20228803499105	37.0	37.0	37.0	35.0	37.0
6	36.35101665756764	37.0	37.0	37.0	35.0	37.0
7	36.29618761347333	37.0	37.0	37.0	35.0	37.0
8	36.20954080596219	37.0	37.0	37.0	35.0	37.0
9	37.74315033708721	39.0	38.0	39.0	35.0	39.0
10	37.56932823787819	39.0	37.0	39.0	35.0	39.0
11	37.32406555108363	39.0	37.0	39.0	35.0	39.0
12	37.33856712542691	39.0	37.0	39.0	35.0	39.0
13	37.34634361946599	39.0	37.0	39.0	35.0	39.0
14	38.70176351840331	40.0	38.0	41.0	35.0	41.0
15	38.77924437870574	41.0	38.0	41.0	35.0	41.0
16	38.70330294681513	40.0	38.0	41.0	35.0	41.0
17	38.66572661021038	40.0	38.0	41.0	34.0	41.0
18	38.50717976714955	40.0	38.0	41.0	35.0	41.0
19	38.297358055178194	40.0	37.0	41.0	35.0	41.0
20	38.03353335322423	40.0	35.0	41.0	34.0	41.0
21	37.972049852087906	40.0	35.0	41.0	34.0	41.0
22	37.942412681081215	40.0	35.0	41.0	34.0	41.0
23	37.89254948389472	40.0	35.0	41.0	34.0	41.0
24	37.81254285006919	40.0	35.0	41.0	34.0	41.0
25	37.754600827799855	40.0	35.0	41.0	34.0	41.0
26	37.722514061170855	40.0	35.0	41.0	34.0	41.0
27	37.70367669464088	40.0	35.0	41.0	34.0	41.0
28	37.60356941076394	40.0	35.0	41.0	34.0	41.0
29	37.56830697789571	39.0	35.0	41.0	34.0	41.0
30	37.46037558879169	40.0	35.0	41.0	34.0	41.0
31	37.35711327019032	40.0	35.0	41.0	33.0	41.0
32	37.129527823978265	40.0	35.0	41.0	33.0	41.0
33	36.83127150438658	39.0	35.0	41.0	32.0	41.0
34	36.599970004951565	39.0	35.0	41.0	31.0	41.0
35	36.43759522237599	39.0	35.0	41.0	31.0	41.0
36	36.353440860556354	39.0	35.0	41.0	31.0	41.0
37	36.24844628823178	39.0	35.0	41.0	31.0	41.0
38	36.13388504119955	39.0	35.0	41.0	31.0	41.0
39	36.035402885872806	39.0	35.0	41.0	30.0	41.0
40	35.91309053743509	38.0	35.0	41.0	30.0	41.0
41	35.81866882292447	38.0	35.0	41.0	30.0	41.0
42	35.6925626563234	38.0	35.0	40.0	29.0	41.0
43	35.57060421771644	38.0	35.0	40.0	29.0	41.0
44	35.44036778055686	37.0	35.0	40.0	29.0	41.0
45	35.357378305803486	37.0	35.0	40.0	28.0	41.0
46	35.2246184756802	37.0	35.0	40.0	28.0	41.0
47	35.066930221042874	37.0	35.0	40.0	27.0	41.0
48	34.98251717176847	36.0	35.0	40.0	27.0	41.0
49	34.89007132155962	36.0	35.0	40.0	27.0	41.0
50	34.72533899165852	36.0	35.0	40.0	27.0	41.0
51	34.281287692190496	35.0	34.0	39.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	0.0
13	4.0
14	7.0
15	19.0
16	47.0
17	95.0
18	211.0
19	422.0
20	829.0
21	1412.0
22	2201.0
23	3475.0
24	5710.0
25	9797.0
26	15154.0
27	19450.0
28	20171.0
29	19930.0
30	20689.0
31	22983.0
32	27292.0
33	36221.0
34	79216.0
35	128623.0
36	109818.0
37	117183.0
38	193566.0
39	425598.0
40	83.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.467979889034194	17.383082792681844	22.621503751761615	28.527433566522355
2	34.14047522313777	19.687384939629013	24.802889681703338	21.36925015552988
3	26.16861660932164	20.980822213475868	32.76816208118025	20.082399096022243
4	23.966678516562347	23.917004177088227	30.543529322143648	21.57278798420578
5	21.787038330180415	31.838077523710375	28.400470398537387	17.97441374757183
6	78.93062097685461	1.8893706435762985	16.304451328669554	2.875557050899534
7	79.12876287089115	2.1027481177710348	14.521332986300676	4.247156025037137
8	69.95956223099678	3.4981526859058185	19.97519457105494	6.567090512042456
9	39.35945494711984	16.917604078056954	25.61410497314729	18.10883600167591
10	27.683525259322273	17.832929167248583	34.81552251691784	19.668023056511306
11	24.699017939895636	15.200030471160316	38.3260541117022	21.774897477241854
12	20.31283724591496	17.972112540152104	40.51362949608318	21.20142071784975
13	16.21319655168036	21.416940695504234	41.974102687810266	20.395760065005142
14	16.386898035879792	19.83648731003136	41.790244150172036	21.986370503916813
15	19.507494000990313	16.059650470398537	42.48013026421035	21.952725264400797
16	18.080427992839276	19.906634460343053	37.75797328694945	24.25496425986821
17	19.431792212079277	19.898381854424034	37.246232367990046	23.423593565506646
18	20.902977921105087	25.027852544976703	34.11920889250028	19.949960641417924
19	22.20498520879093	21.145001460076433	33.101043637240835	23.548969693891802
20	20.511058491931493	25.221312672193797	34.948516435382096	19.319112400492617
21	19.4822600713533	19.98860505567335	35.535562383352584	24.99357248962076
22	19.532727930627324	21.68634066757234	32.49471515813263	26.28621624366771
23	19.88901832078514	20.292205731117402	38.29423396772596	21.524541980371495
24	23.627052042202557	18.635177684953593	33.80219773243782	23.935572540406028
25	17.937356372916216	21.934394957022967	34.494623109835835	25.633625560224978
26	18.064398892881176	20.264591242080673	34.764181785863926	26.906828079174232
27	21.930348006043445	20.971934791716922	34.6520574381372	22.445659764102434
28	21.575247895585488	21.386707591127813	32.50209489227176	24.535949621014943
29	23.230530198189506	19.68048131736983	33.942333329101224	23.146655155339435
30	24.05523532623186	20.27736691085916	30.81372281401166	24.853674948897325
31	27.73835747749578	19.380689536965328	29.871497403603215	23.009455581935683
32	24.89128778741287	20.09112781382121	30.349116971166662	24.668467427599253
33	20.948922717519647	20.892344755786347	32.09486053095997	26.06387199573404
34	21.830205807295304	20.385603011566346	34.54628124880972	23.23790993232863
35	23.291710574762263	24.06293246829095	29.996159364168456	22.649197592778336
36	21.8885295125884	23.85995010347498	30.938860886456837	23.312659497479782
37	21.370043675329786	25.252974112210048	32.39330332770463	20.983678884755534
38	19.849183626829856	24.87906758249432	29.782147074133793	25.489601716542033
39	22.734580323248228	22.42177481812526	33.036689181468454	21.806955677158058
40	18.66985450020949	21.513750111092772	34.845041453474344	24.97135393522339
41	21.314338585376383	24.75702423726877	28.567903076317556	25.36073410103729
42	20.63032451785737	19.75800820182065	32.51145842591065	27.100208854411335
43	23.140703756840143	19.805540037835023	32.7305492426647	24.323206962660134
44	21.58826162030395	20.907500983964553	33.2255468938461	24.278690501885404
45	22.22998108248797	21.074457549864782	32.10446212053883	24.591099247108414
46	22.37543326181075	21.751091883244673	31.913382552721455	23.960092302223124
47	19.395210949303607	22.534851389611873	34.665150514835645	23.40478714624887
48	21.483755062656325	20.63976740347625	31.077171387580467	26.79930614628696
49	21.19332681589071	18.935604281198025	34.82544151441667	25.045627388494594
50	20.002412300191715	19.315700265353023	35.647051915239395	25.034835519215875
51	19.35870903850793	18.121611670454403	34.18173825273288	28.337941038304788
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	401.0
1	2310.5
2	4220.0
3	34334.0
4	64448.0
5	36941.5
6	9435.0
7	8595.5
8	7756.0
9	7651.5
10	7547.0
11	7428.5
12	7310.0
13	6966.0
14	6622.0
15	6249.0
16	5876.0
17	5542.5
18	5209.0
19	4860.5
20	4512.0
21	4444.5
22	4377.0
23	4395.0
24	4413.0
25	4627.5
26	5335.5
27	5829.0
28	6737.5
29	7646.0
30	9439.5
31	11233.0
32	12396.0
33	13559.0
34	15476.5
35	17394.0
36	19484.0
37	21574.0
38	23525.5
39	25477.0
40	29855.0
41	34233.0
42	39342.5
43	44452.0
44	51542.5
45	58633.0
46	71470.0
47	84307.0
48	106092.0
49	127877.0
50	132179.5
51	136482.0
52	115271.0
53	94060.0
54	84443.5
55	74827.0
56	69809.0
57	64791.0
58	61647.0
59	58503.0
60	56224.5
61	53946.0
62	50998.5
63	48051.0
64	46733.5
65	45416.0
66	37535.5
67	29655.0
68	24357.0
69	19059.0
70	16151.0
71	13243.0
72	13128.0
73	13013.0
74	11227.0
75	7364.5
76	5288.0
77	3875.5
78	2463.0
79	1927.0
80	1391.0
81	1034.0
82	677.0
83	539.0
84	401.0
85	306.0
86	211.0
87	137.0
88	63.0
89	44.0
90	25.0
91	15.5
92	6.0
93	8.0
94	10.0
95	7.0
96	4.0
97	2.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1260208.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.921106613407783
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.06948405363067	21.421714451151605
2	10.70974982833975	6.194756331766793
3	4.0152208506881495	3.4837389089738964
4	1.9347004887924517	2.2381471640551456
5	1.1096193435914095	1.604570966815336
6	0.7340071803578543	1.2736979950881802
7	0.5571641784070226	1.1279663223416878
8	0.43604928614545246	1.008882231465039
9	0.37854411394389537	0.9853123209524443
>10	5.428637495735635	37.194466985127285
>50	0.516306259677931	9.572978047043268
>100	0.09888356061927271	5.204395395353631
>500	0.007201603853173581	1.4856655121136322
>1k	0.0036008019265867906	2.0553869141492274
>5k	5.539695271671985E-4	1.0875334299824582
>10k+	2.7698476358359927E-4	4.060787023620416
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	50692	4.022510569683735	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	7997	0.634577783984866	No Hit
CCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCTGC	5579	0.44270469636758375	No Hit
CTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCTGCT	4671	0.37065309853611467	No Hit
GCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCTGC	3660	0.2904282467656133	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG	3476	0.275827482447342	No Hit
CGCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCTG	2093	0.1660836941203357	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCC	1770	0.14045300458337034	No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	1577	0.12513807244518366	No Hit
TCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCTGC	1459	0.1157745388062923	No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	1380	0.10950573238703452	No Hit
GCCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCTG	1358	0.10775998882724122	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.38200043167477116	0.0
2	0.0	0.0	0.0	1.3223213945634371	0.0
3	0.0	0.0	0.0	2.1060015489506494	0.0
4	0.0	0.0	0.0	2.6164728362302094	0.0
5	0.0	0.0	0.0	5.23969059075962	0.0
6	0.0	0.0	0.0	5.804438632352754	0.0
7	0.0	0.0	0.0	7.756179932201668	0.0
8	0.0	0.0	0.0	10.02929675101253	0.0
9	0.0	0.0	0.0	11.839712174498178	0.0
10	0.0	0.0	0.0	13.122198875106331	0.0
11	0.0	0.0	0.0	14.293434099767657	0.0
12	0.0	0.0	0.0	15.167258103424196	0.0
13	0.0	0.0	0.0	15.59417175577365	0.0
14	0.0	0.0	0.0	15.883012962939452	0.0
15	0.0	0.0	0.0	16.833094219366963	0.0
16	0.0	0.0	0.0	17.837769638028007	0.0
17	7.935197999060473E-5	0.0	0.0	19.008687454769372	0.0
18	7.935197999060473E-5	0.0	0.0	19.66588055305156	0.0
19	7.935197999060473E-5	0.0	0.0	20.341086550791616	0.0
20	7.935197999060473E-5	0.0	0.0	21.12389383339893	0.0
21	7.935197999060473E-5	0.0	0.0	21.860042151771772	0.0
22	2.3805593997181418E-4	0.0	0.0	22.595317598364716	0.0
23	3.174079199624189E-4	0.0	0.0	23.283140560923275	0.0
24	6.348158399248379E-4	0.0	0.0	23.79829361502228	0.0
25	8.72871779896652E-4	0.0	0.0	24.30471795132232	0.0
26	8.72871779896652E-4	0.0	0.0	24.791859756484644	0.0
27	8.72871779896652E-4	0.0	0.0	25.31359902492287	0.0
28	8.72871779896652E-4	0.0	0.0	25.853589248758936	0.0
29	8.72871779896652E-4	0.0	0.0	26.40127661465409	0.0
30	8.72871779896652E-4	0.0	0.0	26.925475794472025	0.0
31	8.72871779896652E-4	0.0	0.0	27.408094536774882	0.0
32	8.72871779896652E-4	0.0	0.0	27.947846704670976	0.0
33	8.72871779896652E-4	0.0	0.0	28.442368243972425	0.0
34	8.72871779896652E-4	0.0	0.0	28.994419968767062	0.0
35	8.72871779896652E-4	0.0	0.0	29.44315541561393	0.0
36	8.72871779896652E-4	0.0	0.0	29.930455924736233	0.0
37	8.72871779896652E-4	0.0	0.0	30.47290605995201	0.0
38	8.72871779896652E-4	0.0	0.0	31.02836991988624	0.0
39	8.72871779896652E-4	0.0	0.0	31.67389827710981	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATAACGG	60	0.0	45.000004	1
AATCGAG	35	1.2120472E-7	45.000004	30
CGGAATA	25	3.8910206E-5	45.0	5
CCCGTTA	25	3.8910206E-5	45.0	42
GCCGATT	25	3.8910206E-5	45.0	8
CGCTAAT	20	7.033547E-4	45.0	33
CTTGACG	20	7.033547E-4	45.0	11
ACGTCGA	20	7.033547E-4	45.0	29
TATACGG	50	2.1827873E-11	45.0	1
CGTTTAG	20	7.033547E-4	45.0	28
GCGTAGG	165	0.0	45.0	1
CGTTTTT	12110	0.0	44.163914	1
CGGTCTA	290	0.0	43.448273	30
CGACGGT	300	0.0	42.750004	27
TACGAAA	180	0.0	42.5	19
CGTAGGG	525	0.0	42.428574	2
CACGACG	320	0.0	42.1875	25
CTCACGT	245	0.0	41.32653	44
GTAGGGT	540	0.0	41.25	3
GCACCGA	290	0.0	41.12069	8
>>END_MODULE