FastQCFastQC Report
Thu 26 May 2016
SRR1043356_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1043356_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2923522
Sequences flagged as poor quality0
Sequence length52
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1020383.4902422489038907No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC178490.6105307228746697No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA134590.4603693763891635No Hit
CCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCTGCT106350.3637735580577126No Hit
CTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCTGCTT87490.2992623281097252Illumina Single End Adapter 1 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCTGCT60120.20564237245349956No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC54720.18717150067623914No Hit
CGCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCTGC49130.168050727854964No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC39540.13524782779127367No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCG34230.11708480387696758No Hit
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC30510.10436042554152149No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGATAA301.862396E-646.011
CGTTTTT272500.045.3078881
ACGTAGG2250.043.9555551
AATGCGG4150.042.67471
ATTACGG2050.042.6341481
CACGACG4250.042.21176525
GTACCGG1800.042.1666681
ATAACGG1700.041.9411771
TAGTAGG5650.041.9292031
ACGGGTA2200.041.818184
GTTACGG2200.041.818181
CTAACGG2600.041.5769231
ATAGCGG4600.041.51
GCACTTA46650.041.2175835
TAGCATA18850.040.9973529
CGCACTT46950.040.95420534
CACTTAC46950.040.95420536
ACCCGCA47300.040.94291731
ATACCGG900.040.888891
GCGCGAC20500.040.8390278
Produced by FastQC (version 0.11.5)