##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043356_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2923522
Sequences flagged as poor quality	0
Sequence length	52
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.39161258235785	33.0	31.0	34.0	31.0	34.0
2	32.72947732221615	34.0	31.0	34.0	31.0	34.0
3	32.86152387428588	34.0	31.0	34.0	31.0	34.0
4	36.258166348671224	37.0	37.0	37.0	35.0	37.0
5	36.20310365374367	37.0	37.0	37.0	35.0	37.0
6	36.34595532374991	37.0	37.0	37.0	35.0	37.0
7	36.29250883010287	37.0	37.0	37.0	35.0	37.0
8	36.27500391650892	37.0	37.0	37.0	35.0	37.0
9	37.89069143314126	39.0	38.0	39.0	35.0	39.0
10	37.65713136415597	39.0	37.0	39.0	35.0	39.0
11	37.23712084260013	39.0	35.0	39.0	35.0	39.0
12	37.22641628829884	39.0	37.0	39.0	35.0	39.0
13	37.164506372792815	39.0	37.0	39.0	34.0	39.0
14	38.37050071796963	40.0	36.0	41.0	34.0	41.0
15	38.48914870488404	40.0	37.0	41.0	35.0	41.0
16	38.35992443361124	40.0	37.0	41.0	34.0	41.0
17	38.296629202721924	40.0	36.0	41.0	34.0	41.0
18	38.181351465800496	40.0	36.0	41.0	34.0	41.0
19	38.004195966372066	40.0	36.0	41.0	34.0	41.0
20	37.67593984242294	40.0	35.0	41.0	34.0	41.0
21	37.6717199323282	39.0	35.0	41.0	34.0	41.0
22	37.64594006817804	39.0	35.0	41.0	34.0	41.0
23	37.65250509488213	39.0	35.0	41.0	34.0	41.0
24	37.59550261636478	39.0	35.0	41.0	34.0	41.0
25	37.485489419953055	39.0	35.0	41.0	34.0	41.0
26	37.38321346649692	39.0	35.0	41.0	34.0	41.0
27	37.42578198487988	39.0	35.0	41.0	34.0	41.0
28	37.43771074751618	39.0	35.0	41.0	34.0	41.0
29	37.343528798483476	39.0	35.0	41.0	33.0	41.0
30	37.28164727339148	39.0	35.0	41.0	33.0	41.0
31	37.20197214182072	39.0	35.0	41.0	33.0	41.0
32	36.96058213346778	39.0	35.0	41.0	33.0	41.0
33	36.493873485474026	39.0	35.0	41.0	31.0	41.0
34	36.37037107981401	39.0	35.0	41.0	31.0	41.0
35	36.26130673892654	39.0	35.0	41.0	31.0	41.0
36	36.227733535099105	39.0	35.0	41.0	31.0	41.0
37	36.172653395459314	39.0	35.0	41.0	31.0	41.0
38	36.09367947290973	38.0	35.0	41.0	31.0	41.0
39	36.046720701947855	38.0	35.0	41.0	31.0	41.0
40	35.951492070181104	38.0	35.0	41.0	31.0	41.0
41	35.91086196717521	38.0	35.0	41.0	31.0	41.0
42	35.81596375878136	38.0	35.0	41.0	31.0	41.0
43	35.70857650464063	37.0	35.0	41.0	30.0	41.0
44	35.52798234458301	37.0	35.0	40.0	30.0	41.0
45	35.429437849279054	37.0	35.0	40.0	30.0	41.0
46	35.40673372733299	37.0	35.0	40.0	30.0	41.0
47	35.32906610588188	36.0	35.0	40.0	30.0	41.0
48	35.23829647938343	36.0	35.0	40.0	29.0	41.0
49	35.19117454905419	36.0	35.0	40.0	29.0	41.0
50	35.10707051289506	36.0	35.0	40.0	29.0	41.0
51	34.987560209911194	35.0	35.0	40.0	29.0	41.0
52	34.614801598893386	35.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	5.0
12	5.0
13	12.0
14	23.0
15	54.0
16	128.0
17	233.0
18	503.0
19	1126.0
20	1983.0
21	3289.0
22	5107.0
23	8061.0
24	13605.0
25	24083.0
26	35699.0
27	42704.0
28	44910.0
29	45355.0
30	48301.0
31	55764.0
32	66742.0
33	89061.0
34	231617.0
35	320931.0
36	254465.0
37	280326.0
38	392716.0
39	951864.0
40	4849.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.156578948268564	18.150709999787924	24.36677404856197	28.325937003381537
2	34.060253351950145	20.079069013333918	25.164407861476672	20.696269773239266
3	26.96121322158684	21.16252246434267	32.680547640825004	19.19571667324549
4	24.378164419491284	23.593391806184457	30.52855425750174	21.499889516822517
5	23.08386938767692	31.126018548859903	27.45363298104136	18.336479082421818
6	79.82211182265773	2.0483854747800767	15.457656894663355	2.671845807898829
7	79.8270031831469	1.9534657170358218	14.011113991959014	4.208417107858262
8	70.78007280259907	3.3600568081923106	19.14358776845189	6.716282620756744
9	46.4870454198737	14.063413923343147	23.104016320041374	16.34552433674178
10	31.162447212642835	14.939925199810366	32.01911940460855	21.87850818293825
11	27.399793810342455	12.149079090220631	37.010154190732955	23.44097290870395
12	23.361684981334157	13.821000833925655	39.481180576031235	23.33613360870895
13	18.240225317271427	16.934916173026917	43.74148715145636	21.083371358245294
14	18.607350996503534	16.323051442746113	43.68590350953404	21.383694051216306
15	23.75928759899874	15.108591623391238	40.256033647087314	20.876087130522706
16	22.92813257434013	17.842896342151693	36.44012256449584	22.78884851901234
17	22.575783592529834	19.086362271260487	37.44640881785736	20.891445318352318
18	23.971907856345872	24.367116101743033	32.905276580781674	18.755699461129417
19	24.77628011692746	20.979352985884834	31.263250285101325	22.98111661208638
20	22.825414004067696	24.878964481881784	34.19710882969241	18.098512684358113
21	21.59149135871049	19.2190104948757	34.882036119447704	24.307462026966103
22	23.524946964654276	19.86788537934724	32.46224930067227	24.14491835532621
23	23.528743754964047	18.705486054149755	34.46743345868442	23.298336732201776
24	26.50087805051578	16.555202936731792	31.56199953343946	25.381919479312963
25	18.68834918977863	18.396509415697913	35.163238039597445	27.75190335492601
26	17.87268917422205	19.313143530303517	35.489488363692836	27.324678931781598
27	21.216327429723464	21.626722836359704	33.71088023281508	23.446069501101753
28	24.24014596093342	22.29427382451714	30.803051935302694	22.662528279246743
29	23.125976134265454	18.051446166644205	35.7402475507282	23.082330148362146
30	26.344662362725508	20.162701016103178	30.46691627427466	23.025720346896655
31	28.432828622462907	17.66311319018636	32.10312766587698	21.800930521473756
32	28.202626831609273	19.397562255389218	30.115319809462697	22.284491103538816
33	19.847088545938767	21.16833736842069	36.52676463525843	22.457809450382108
34	21.388038126615776	23.599138299626272	34.57353835544935	20.4392852183086
35	24.619140885548322	24.17758443411748	29.44140663213754	21.76186804819666
36	25.08895092973475	23.935787040425897	28.865286459277545	22.109975570561808
37	22.565556202416126	25.444344184856483	29.714843945077206	22.275255667650185
38	21.529955991437724	21.89249815804362	28.919330861885083	27.658214988633574
39	22.628767630276084	19.784082349987447	32.88314573996707	24.7040042797694
40	18.565894150959014	21.01020618281648	34.58838346350737	25.835516202717134
41	20.31419636999482	23.25007302835416	29.61294630243932	26.822784299211705
42	19.65817257403912	18.51362842489299	32.10258038078728	29.72561862028061
43	22.048782256470105	18.90226924921379	33.599644538334246	25.44930395598186
44	23.475109816173777	19.676369803271534	32.37677021072528	24.471750169829402
45	21.940454014028283	21.36580466984685	31.914793184385136	24.77894813173973
46	22.053434179732527	20.61161161092682	32.05937906401935	25.275575145321294
47	20.522814605123546	20.61681081927894	34.30311111050302	24.557263465094497
48	22.76151846984562	19.33298261480502	31.150099092806556	26.75539982254281
49	20.932183852216607	18.44655179608705	34.800764283627764	25.82050006806858
50	20.56057727631261	17.95885237053116	35.89488979388559	25.585680559270635
51	19.83456939951196	16.623374135717125	34.23524775938064	29.30680870539028
52	17.659795274330072	17.54903845430272	38.067201136163845	26.723965135203358
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	765.0
1	5114.5
2	9464.0
3	76678.0
4	143892.0
5	82493.0
6	21094.0
7	19168.5
8	17243.0
9	16896.0
10	16549.0
11	15976.5
12	15404.0
13	14574.0
14	12954.5
15	12165.0
16	11720.0
17	11275.0
18	10707.0
19	10139.0
20	9688.0
21	9237.0
22	8788.0
23	8339.0
24	8540.0
25	8741.0
26	9167.0
27	9593.0
28	10130.0
29	10667.0
30	11927.0
31	13187.0
32	15427.5
33	17668.0
34	20884.0
35	24100.0
36	28138.0
37	32176.0
38	34354.0
39	41012.5
40	45493.0
41	60151.5
42	74810.0
43	103701.0
44	132592.0
45	173099.5
46	213607.0
47	236194.5
48	258782.0
49	255239.0
50	251696.0
51	232624.0
52	213552.0
53	191540.0
54	169528.0
55	165281.0
56	161034.0
57	159015.5
58	156997.0
59	157208.5
60	157420.0
61	156479.0
62	155538.0
63	155402.0
64	135686.5
65	116107.0
66	97457.5
67	78808.0
68	63026.5
69	47245.0
70	38875.5
71	30506.0
72	28719.0
73	26932.0
74	21721.5
75	16511.0
76	12859.5
77	9208.0
78	7240.0
79	5272.0
80	3896.0
81	2520.0
82	1867.0
83	1214.0
84	822.5
85	431.0
86	326.5
87	222.0
88	158.0
89	78.0
90	62.0
91	51.0
92	40.0
93	45.5
94	51.0
95	30.5
96	10.0
97	5.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	2923522.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.682717344688527
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.06385145176048	18.507955276051863
2	11.250431496990739	5.778833042859883
3	4.43947238362664	3.420531431647009
4	2.2161429828573476	2.276662952965607
5	1.312091230640971	1.6849034103498288
6	0.8725786872380714	1.3446115071200855
7	0.6294677640961168	1.1316509864011552
8	0.5019439356246568	1.0313027377222874
9	0.3940951991686062	0.9109292046431439
>10	5.303924231284626	32.63743521404963
>50	0.8226765078807725	13.783902431608496
>100	0.17979674989906683	8.038950843160354
>500	0.007366394467906178	1.310116812755876
>1k	0.005223443349969832	2.4935801714574244
>5k	4.0180333461306404E-4	0.6959748332105344
>10k+	5.357377794840855E-4	4.952659143996737
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	102038	3.4902422489038907	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	17849	0.6105307228746697	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA	13459	0.4603693763891635	No Hit
CCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCTGCT	10635	0.3637735580577126	No Hit
CTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCTGCTT	8749	0.2992623281097252	Illumina Single End Adapter 1 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCTGCT	6012	0.20564237245349956	No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	5472	0.18717150067623914	No Hit
CGCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCTGC	4913	0.168050727854964	No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	3954	0.13524782779127367	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCG	3423	0.11708480387696758	No Hit
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	3051	0.10436042554152149	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.3070269353197958	0.0
2	0.0	0.0	0.0	1.036386933294841	0.0
3	0.0	0.0	0.0	1.6715454852058578	0.0
4	0.0	0.0	0.0	2.0930576202265625	0.0
5	0.0	0.0	0.0	4.278606420611851	0.0
6	0.0	0.0	0.0	4.97478726002404	0.0
7	0.0	0.0	0.0	7.081834855355972	0.0
8	0.0	0.0	0.0	9.653219643977367	0.0
9	0.0	0.0	0.0	11.769434264561717	0.0
10	0.0	0.0	0.0	13.113327007629838	0.0
11	0.0	0.0	0.0	14.26970619684066	0.0
12	0.0	0.0	0.0	15.073633788286868	0.0
13	0.0	0.0	0.0	15.502397450746052	0.0
14	0.0	0.0	0.0	15.770430323424964	0.0
15	0.0	0.0	0.0	16.46370371079814	0.0
16	0.0	0.0	0.0	17.234075885182325	0.0
17	3.420531810603786E-5	0.0	0.0	18.2559255582821	0.0
18	3.420531810603786E-5	0.0	0.0	18.874494530911686	0.0
19	3.420531810603786E-5	0.0	0.0	19.52446398556262	0.0
20	3.420531810603786E-5	0.0	0.0	20.32415011756368	0.0
21	3.420531810603786E-5	0.0	0.0	21.088741593187944	0.0
22	6.841063621207572E-5	0.0	0.0	21.755198011165984	0.0
23	6.841063621207572E-5	0.0	0.0	22.398600044740554	0.0
24	1.3682127242415143E-4	0.0	0.0	22.892148579692577	0.0
25	1.3682127242415143E-4	0.0	0.0	23.41891047852556	0.0
26	1.3682127242415143E-4	0.0	0.0	23.8978533426463	0.0
27	1.3682127242415143E-4	0.0	0.0	24.409564901512628	0.0
28	1.3682127242415143E-4	0.0	0.0	24.910672811766084	0.0
29	1.3682127242415143E-4	0.0	0.0	25.420674104727105	0.0
30	1.3682127242415143E-4	0.0	0.0	25.955611074587434	0.0
31	1.3682127242415143E-4	0.0	0.0	26.46232865701028	0.0
32	1.3682127242415143E-4	0.0	0.0	27.03232607792929	0.0
33	1.3682127242415143E-4	0.0	0.0	27.56042198416841	0.0
34	1.3682127242415143E-4	0.0	0.0	28.114240289623268	0.0
35	1.3682127242415143E-4	0.0	0.0	28.616271743465585	0.0
36	1.3682127242415143E-4	0.0	0.0	29.17118461909984	0.0
37	1.3682127242415143E-4	0.0	0.0	29.900544617074885	0.0
38	1.7102659053018927E-4	0.0	0.0	30.789985503786188	0.0
39	1.7102659053018927E-4	0.0	0.0	31.65421707105334	0.0
40	1.7102659053018927E-4	0.0	0.0	32.437279418454864	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGATAA	30	1.862396E-6	46.0	11
CGTTTTT	27250	0.0	45.307888	1
ACGTAGG	225	0.0	43.955555	1
AATGCGG	415	0.0	42.6747	1
ATTACGG	205	0.0	42.634148	1
CACGACG	425	0.0	42.211765	25
GTACCGG	180	0.0	42.166668	1
ATAACGG	170	0.0	41.941177	1
TAGTAGG	565	0.0	41.929203	1
ACGGGTA	220	0.0	41.81818	4
GTTACGG	220	0.0	41.81818	1
CTAACGG	260	0.0	41.576923	1
ATAGCGG	460	0.0	41.5	1
GCACTTA	4665	0.0	41.21758	35
TAGCATA	1885	0.0	40.99735	29
CGCACTT	4695	0.0	40.954205	34
CACTTAC	4695	0.0	40.954205	36
ACCCGCA	4730	0.0	40.942917	31
ATACCGG	90	0.0	40.88889	1
GCGCGAC	2050	0.0	40.839027	8
>>END_MODULE