FastQCFastQC Report
Thu 26 May 2016
SRR1043355_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1043355_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2878763
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT972213.3771797122583553No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC170510.5923030134818323No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG113310.3936065594840562No Hit
CCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCTGC104420.3627252399728634No Hit
CTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCTGCT87800.3049921094581249No Hit
GCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCTGC63040.21898294510524136No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC51160.1777152200441648No Hit
CGCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCTG48520.16854461447503669No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC37940.13179271791390954No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCC32390.1125136039333561No Hit
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC30860.10719882116033866No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTCG453.8562575E-1045.026
CGTTTTT264700.044.4304851
TATACGG1750.043.7142871
GTTACGG2050.042.804881
AGGGTAC10500.042.4285745
CATACCA850.042.3529418
GTACCGG750.042.01
TACCGGT1450.041.89655340
CGCACTT43700.041.3958834
GCACTTA43700.041.29290835
CCGCACT43850.041.20296533
AATGCGG4000.041.06251
CACTTAC43900.041.05352836
CCCGCAC44600.040.91367732
ACTTACT43850.040.89509637
ACCCGCA44650.040.8174731
TAGCATA19700.040.5456929
ACGGGAT3900.040.3846174
CTTACTG44350.040.3325838
ACGTAGG1900.040.2631571