##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043355_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2878763
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.39795460758666	33.0	31.0	34.0	31.0	34.0
2	32.68100604322065	34.0	31.0	34.0	31.0	34.0
3	32.7873694361085	34.0	31.0	34.0	31.0	34.0
4	36.23821690080079	37.0	37.0	37.0	35.0	37.0
5	36.2255975917434	37.0	37.0	37.0	35.0	37.0
6	36.36694198167755	37.0	37.0	37.0	35.0	37.0
7	36.32563778261705	37.0	37.0	37.0	35.0	37.0
8	36.23312061465289	37.0	37.0	37.0	35.0	37.0
9	37.85880185343496	39.0	38.0	39.0	35.0	39.0
10	37.6126933686448	39.0	37.0	39.0	35.0	39.0
11	37.23768438040923	39.0	35.0	39.0	35.0	39.0
12	37.23165852833318	39.0	37.0	39.0	35.0	39.0
13	37.18533550695212	39.0	37.0	39.0	34.0	39.0
14	38.39770658439059	40.0	36.0	41.0	34.0	41.0
15	38.521113756151514	40.0	37.0	41.0	35.0	41.0
16	38.43242635812674	40.0	37.0	41.0	34.0	41.0
17	38.345431353675174	40.0	36.0	41.0	34.0	41.0
18	38.24478187332545	40.0	36.0	41.0	34.0	41.0
19	38.08716070062037	40.0	36.0	41.0	34.0	41.0
20	37.78508130054471	40.0	35.0	41.0	34.0	41.0
21	37.77527361578567	40.0	35.0	41.0	34.0	41.0
22	37.76780895127525	39.0	35.0	41.0	34.0	41.0
23	37.74456459250032	39.0	35.0	41.0	34.0	41.0
24	37.68617006679605	39.0	35.0	41.0	34.0	41.0
25	37.6405487356896	39.0	35.0	41.0	34.0	41.0
26	37.61834927015527	39.0	35.0	41.0	34.0	41.0
27	37.59869916349488	39.0	35.0	41.0	34.0	41.0
28	37.53535945821174	39.0	35.0	41.0	34.0	41.0
29	37.48330307149286	39.0	35.0	41.0	34.0	41.0
30	37.38309892130752	39.0	35.0	41.0	34.0	41.0
31	37.28969491410026	39.0	35.0	41.0	33.0	41.0
32	37.06522315313904	39.0	35.0	41.0	33.0	41.0
33	36.612336618193304	39.0	35.0	41.0	32.0	41.0
34	36.434506070836676	39.0	35.0	41.0	31.0	41.0
35	36.3216624640514	39.0	35.0	41.0	31.0	41.0
36	36.264662634610765	39.0	35.0	41.0	31.0	41.0
37	36.165701032005764	38.0	35.0	41.0	31.0	41.0
38	36.08237670138181	38.0	35.0	41.0	31.0	41.0
39	35.99965679703401	38.0	35.0	41.0	31.0	41.0
40	35.890856246241874	38.0	35.0	41.0	31.0	41.0
41	35.81075204870981	38.0	35.0	41.0	31.0	41.0
42	35.695013448484644	37.0	35.0	40.0	30.0	41.0
43	35.58021136161608	37.0	35.0	40.0	30.0	41.0
44	35.46524079960733	37.0	35.0	40.0	30.0	41.0
45	35.39371042353955	37.0	35.0	40.0	30.0	41.0
46	35.31580717134408	37.0	35.0	40.0	29.0	41.0
47	35.17934334990411	36.0	35.0	40.0	29.0	41.0
48	35.119071976400974	36.0	35.0	40.0	29.0	41.0
49	35.05519106644069	36.0	35.0	40.0	29.0	41.0
50	34.99415165472114	36.0	35.0	40.0	29.0	41.0
51	34.58976928632194	35.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	2.0
13	6.0
14	24.0
15	58.0
16	103.0
17	237.0
18	490.0
19	933.0
20	1751.0
21	2940.0
22	4563.0
23	7066.0
24	11598.0
25	20589.0
26	32563.0
27	41310.0
28	44600.0
29	44685.0
30	47583.0
31	54570.0
32	63935.0
33	86604.0
34	224786.0
35	318874.0
36	244474.0
37	277230.0
38	409318.0
39	937311.0
40	559.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.411313123032357	18.255167236761068	24.44136596169952	27.892153678507054
2	33.864718978255596	20.130382389936234	25.170116470164444	20.834782161643734
3	27.01003868675539	21.25645633211209	32.4498056977945	19.283699283338017
4	24.49729276081428	23.678677265200363	30.21950747595408	21.604522498031272
5	23.266729494578055	31.246128979704128	27.14051139326162	18.3466301324562
6	80.04222647018877	2.037298659181044	15.197534496587597	2.722940374042601
7	80.02982531038505	1.9464957691897526	13.756950467961412	4.266728452463784
8	70.89090001504118	3.3156254960898135	18.977942956749132	6.815531532119873
9	46.60592066800914	14.016263235285434	22.90699164884362	16.470824447861805
10	31.399806097271643	14.883406518702651	31.80418117087096	21.91260621315475
11	27.56694455222608	12.12864692230656	36.82713721136474	23.47727131410262
12	23.458686942968214	13.761570507888285	39.3424884229789	23.437254126164607
13	18.29261387616834	16.869051047272734	43.659168886080586	21.17916619047834
14	18.634948413606818	16.3533086954362	43.54741949927799	21.464323391678995
15	23.806440474606628	15.107461086584758	40.18708730103867	20.89901113776994
16	22.98591443616581	17.83984996333495	36.38903237258503	22.78520322791421
17	22.710101526245822	19.109700937520735	37.307239255193984	20.872958281039462
18	24.01976126551578	24.521330863290935	32.767963184187096	18.69094468700619
19	24.807981761610804	21.116396174329044	30.99855736647998	23.077064697580177
20	22.793887513491036	25.002926604239388	33.9579882053507	18.24519767691887
21	21.535812430547427	19.37214004765241	34.7194611018691	24.37258641993106
22	23.560501507070917	19.9718420724457	32.151170485378614	24.316485935104765
23	23.642099054350773	18.736207183432608	34.23748325235527	23.384210509861354
24	26.551751568295135	16.53751976109183	31.42981898822515	25.480909682387885
25	18.52552641533881	18.416625474205414	35.08395793609964	27.973890174356136
26	17.90407893946115	19.20053856465433	35.39350061120002	27.501881884684497
27	21.28636501163868	21.62220370346569	33.56493744014356	23.526493844752068
28	24.24093265058638	22.359534286080514	30.57302737321551	22.826505690117596
29	23.12673881107962	18.071720388236198	35.53522815181382	23.266312648870365
30	26.436493730119498	20.151641521028303	30.19612937918127	23.21573536967093
31	28.536597142592147	17.59589101291075	31.98252165947666	21.884990185020442
32	28.267766398275928	19.346052453779627	29.981141205441364	22.405039942503084
33	19.8515126114932	21.16047760791701	36.40438618948486	22.583623591104928
34	21.404436558341207	23.59350179226286	34.551437544528675	20.450624104867266
35	24.6315170786897	24.15964773758729	29.294526850595204	21.914308333127806
36	25.107103294018994	23.865354667959814	28.82168486950819	22.205857168513006
37	22.56274656857824	25.279399519863222	29.74767287199398	22.410181039564563
38	21.54147458474352	21.86334894536299	28.81546692103518	27.77970954885831
39	22.605369042189302	19.764113961448025	32.87373778251283	24.75677921384984
40	18.568496260372942	20.989605604907386	34.53827216759421	25.903625967125464
41	20.361245437710572	23.191454107198126	29.527474126907983	26.919826328183323
42	19.79211209814771	18.382860971882714	32.10656799465604	29.718458935313535
43	22.195957082955424	18.76271162301308	33.55597525742828	25.485356036603225
44	23.4205802978571	19.600224124042168	32.41979280684099	24.559402771259737
45	21.98767317768083	21.200077950147335	31.986690116553536	24.8255587556183
46	22.219196231159007	20.562477703096782	31.96567414545762	25.252651920286596
47	20.634592010526745	20.434089225128986	34.28962370295853	24.64169506138574
48	22.897577883278338	19.174902553631544	31.001023703583797	26.92649585950632
49	21.039731301256825	18.32356466996415	34.72237902182292	25.914325006956112
50	20.615625530826957	17.790523221258574	35.954922305170655	25.63892894274381
51	19.849358908670148	16.657501850621255	34.01353289590008	29.479606344808516
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	675.0
1	5164.5
2	9654.0
3	73173.0
4	136692.0
5	79284.5
6	21877.0
7	19523.0
8	17169.0
9	16590.0
10	16011.0
11	15431.0
12	14851.0
13	14023.0
14	13195.0
15	12540.0
16	11885.0
17	11208.0
18	10531.0
19	10072.0
20	9613.0
21	8944.0
22	8275.0
23	8232.5
24	8190.0
25	8122.0
26	8898.0
27	9742.0
28	10112.5
29	10483.0
30	12308.5
31	14134.0
32	16356.0
33	18578.0
34	22734.0
35	26890.0
36	29943.0
37	32996.0
38	36298.0
39	39600.0
40	48268.5
41	56937.0
42	72287.5
43	87638.0
44	120372.0
45	153106.0
46	197628.5
47	242151.0
48	268705.0
49	295259.0
50	266272.5
51	237286.0
52	210603.5
53	183921.0
54	170479.5
55	157038.0
56	157631.5
57	158225.0
58	159093.0
59	159961.0
60	158625.0
61	157289.0
62	155278.5
63	153268.0
64	147446.5
65	141625.0
66	117891.5
67	94158.0
68	75344.5
69	56531.0
70	45975.5
71	35420.0
72	31595.5
73	27771.0
74	23537.0
75	14925.5
76	10548.0
77	8353.0
78	6158.0
79	4789.0
80	3420.0
81	2422.5
82	1425.0
83	1057.5
84	690.0
85	461.5
86	233.0
87	179.5
88	126.0
89	103.5
90	81.0
91	68.0
92	55.0
93	46.5
94	38.0
95	22.5
96	7.0
97	3.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2878763.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.3517424362113
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.18859554986017	18.04754938775673
2	11.63024502740713	5.896939528097056
3	4.584563398755299	3.486800114031775
4	2.300619973305492	2.332989000273766
5	1.3647433342954545	1.7299310751297288
6	0.8802898613337412	1.33901291002447
7	0.6455217918307622	1.1455571542418563
8	0.4956464014168301	1.0052399926523576
9	0.41284680643744714	0.9419747312173089
>10	5.500174582006519	33.326620619739444
>50	0.7984249825202788	13.195868817025527
>100	0.18476806691299497	8.288456020511607
>500	0.007056851222601138	1.2375910949218298
>1k	0.005534785272628342	2.5445335955880037
>5k	4.1510889544712565E-4	0.7085980758906169
>10k+	5.534785272628342E-4	4.772337882897968
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	97221	3.3771797122583553	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	17051	0.5923030134818323	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG	11331	0.3936065594840562	No Hit
CCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCTGC	10442	0.3627252399728634	No Hit
CTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCTGCT	8780	0.3049921094581249	No Hit
GCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCTGC	6304	0.21898294510524136	No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	5116	0.1777152200441648	No Hit
CGCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCTG	4852	0.16854461447503669	No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	3794	0.13179271791390954	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCC	3239	0.1125136039333561	No Hit
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	3086	0.10719882116033866	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.3111753207888249	0.0
2	0.0	0.0	0.0	1.0583365146766164	0.0
3	0.0	0.0	0.0	1.6957283388733286	0.0
4	0.0	0.0	0.0	2.126086794918512	0.0
5	0.0	0.0	0.0	4.379519953535598	0.0
6	0.0	0.0	0.0	5.09833563930063	0.0
7	0.0	0.0	0.0	7.28139134760312	0.0
8	0.0	0.0	0.0	9.96827456793074	0.0
9	0.0	0.0	3.473714230730352E-5	12.1678304188292	0.0
10	0.0	0.0	3.473714230730352E-5	13.560616139640533	0.0
11	0.0	0.0	3.473714230730352E-5	14.744110578050364	0.0
12	0.0	0.0	3.473714230730352E-5	15.56772822215653	0.0
13	0.0	0.0	3.473714230730352E-5	16.018581592163024	0.0
14	0.0	0.0	3.473714230730352E-5	16.2967218906176	0.0
15	0.0	0.0	3.473714230730352E-5	17.02085235915565	0.0
16	0.0	0.0	3.473714230730352E-5	17.805981249585326	0.0
17	0.0	0.0	3.473714230730352E-5	18.84472601600062	0.0
18	0.0	0.0	3.473714230730352E-5	19.475170411735874	0.0
19	0.0	0.0	3.473714230730352E-5	20.148654126789875	0.0
20	0.0	0.0	3.473714230730352E-5	20.970152805215296	0.0
21	0.0	0.0	3.473714230730352E-5	21.753405889960376	0.0
22	1.0421142692191055E-4	0.0	3.473714230730352E-5	22.433975982045066	0.0
23	1.3894856922921407E-4	0.0	3.473714230730352E-5	23.093425891606916	0.0
24	1.736857115365176E-4	0.0	3.473714230730352E-5	23.601352386424306	0.0
25	1.736857115365176E-4	0.0	3.473714230730352E-5	24.140403360749044	0.0
26	1.736857115365176E-4	0.0	3.473714230730352E-5	24.6362760671858	0.0
27	1.736857115365176E-4	0.0	3.473714230730352E-5	25.1537552761377	0.0
28	1.736857115365176E-4	0.0	3.473714230730352E-5	25.662584936655083	0.0
29	1.736857115365176E-4	0.0	3.473714230730352E-5	26.187567368345363	0.0
30	2.4315999615112462E-4	0.0	3.473714230730352E-5	26.727313085516244	0.0
31	2.4315999615112462E-4	0.0	3.473714230730352E-5	27.241318580237415	0.0
32	3.126342807657317E-4	0.0	3.473714230730352E-5	27.81465511401946	0.0
33	3.126342807657317E-4	0.0	3.473714230730352E-5	28.341235454255873	0.0
34	3.126342807657317E-4	0.0	3.473714230730352E-5	28.907277188153383	0.0
35	3.126342807657317E-4	0.0	3.473714230730352E-5	29.414543677266938	0.0
36	3.126342807657317E-4	0.0	3.473714230730352E-5	29.974610622687592	0.0
37	3.126342807657317E-4	0.0	3.473714230730352E-5	30.710551719610123	0.0
38	3.126342807657317E-4	0.0	3.473714230730352E-5	31.61163319106158	0.0
39	3.126342807657317E-4	0.0	3.473714230730352E-5	32.4849944229518	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATTCG	45	3.8562575E-10	45.0	26
CGTTTTT	26470	0.0	44.430485	1
TATACGG	175	0.0	43.714287	1
GTTACGG	205	0.0	42.80488	1
AGGGTAC	1050	0.0	42.428574	5
CATACCA	85	0.0	42.35294	18
GTACCGG	75	0.0	42.0	1
TACCGGT	145	0.0	41.896553	40
CGCACTT	4370	0.0	41.39588	34
GCACTTA	4370	0.0	41.292908	35
CCGCACT	4385	0.0	41.202965	33
AATGCGG	400	0.0	41.0625	1
CACTTAC	4390	0.0	41.053528	36
CCCGCAC	4460	0.0	40.913677	32
ACTTACT	4385	0.0	40.895096	37
ACCCGCA	4465	0.0	40.81747	31
TAGCATA	1970	0.0	40.54569	29
ACGGGAT	390	0.0	40.384617	4
CTTACTG	4435	0.0	40.33258	38
ACGTAGG	190	0.0	40.263157	1
>>END_MODULE