FastQCFastQC Report
Thu 26 May 2016
SRR1043354_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1043354_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2418239
Sequences flagged as poor quality0
Sequence length52
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1261365.216027034548695No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC199770.826097006954234No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA145610.6021323781479002No Hit
CCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTCTGCT97920.40492275577393305No Hit
CTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTCTGCTT69850.28884655321496344Illumina Single End Adapter 2 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTCTGC67770.28024525284721646No Hit
CGTTCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTCT58830.24327620222815033No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC57170.23641170289619845No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTC49810.20597633236417076No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC47590.1967960983178255No Hit
GCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTCTGCT40700.16830429084966372No Hit
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC34190.14138387479484038No Hit
GGGCCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTCT34010.14063953149378536No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCG31600.13067360174077086No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTT31050.12839921943199165No Hit
TGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC27800.11495968760738702No Hit
CGTCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTCTG27560.113967229872647No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAA25320.10470429101507336No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT318250.045.313431
CACTTAC52450.042.14108736
CGGTCTA5950.042.13445330
CTTACTG51850.041.96335638
TCACGAC6200.041.91935324
CGACGGT6000.041.78333327
ACGGGTA2150.041.720934
ACTTACT52650.041.6315337
GCACTTA53050.041.57775535
TCGGCGT21650.041.538114
ACCCGCA54050.041.53191431
CCGCACT53300.041.4690433
CACGACG6050.041.4380225
ACTACGG1000.041.3999981
CGCACTT53350.041.34395634
TACGGGA3900.041.282053
CCCGCAC54900.040.93078632
TCGACGT451.5896148E-840.8888925
GTTTTTT359050.040.676792
TTACTGG52350.040.50811839