##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043354_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2418239
Sequences flagged as poor quality	0
Sequence length	52
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.342004243583865	33.0	31.0	34.0	31.0	34.0
2	32.66682904377938	34.0	31.0	34.0	31.0	34.0
3	32.782717919940914	34.0	31.0	34.0	31.0	34.0
4	36.202934449407195	37.0	37.0	37.0	35.0	37.0
5	36.1593146913932	37.0	37.0	37.0	35.0	37.0
6	36.299223112355726	37.0	37.0	37.0	35.0	37.0
7	36.2464843218557	37.0	37.0	37.0	35.0	37.0
8	36.2358261528327	37.0	37.0	37.0	35.0	37.0
9	37.82448674427962	39.0	38.0	39.0	35.0	39.0
10	37.647789155662444	39.0	37.0	39.0	35.0	39.0
11	37.362791270837995	39.0	37.0	39.0	35.0	39.0
12	37.37789440994046	39.0	37.0	39.0	35.0	39.0
13	37.36425349190051	39.0	37.0	39.0	35.0	39.0
14	38.712464318043004	40.0	38.0	41.0	35.0	41.0
15	38.75912182377341	41.0	38.0	41.0	35.0	41.0
16	38.607468079044295	41.0	38.0	41.0	34.0	41.0
17	38.60154972275279	40.0	38.0	41.0	34.0	41.0
18	38.37934257118506	40.0	38.0	41.0	34.0	41.0
19	38.11109158358624	40.0	37.0	41.0	34.0	41.0
20	37.79285463512912	40.0	35.0	41.0	34.0	41.0
21	37.73225847403834	40.0	35.0	41.0	34.0	41.0
22	37.6829093402265	39.0	35.0	41.0	34.0	41.0
23	37.702594739395074	39.0	35.0	41.0	34.0	41.0
24	37.61297539242399	39.0	35.0	41.0	34.0	41.0
25	37.505237075409006	39.0	35.0	41.0	34.0	41.0
26	37.41402566082178	39.0	35.0	41.0	34.0	41.0
27	37.44475628752989	39.0	35.0	41.0	34.0	41.0
28	37.4022877804882	39.0	35.0	41.0	33.0	41.0
29	37.31604733857985	39.0	35.0	41.0	33.0	41.0
30	37.24226224124249	39.0	35.0	41.0	33.0	41.0
31	37.1265784730128	39.0	35.0	41.0	33.0	41.0
32	36.84569391197479	39.0	35.0	41.0	32.0	41.0
33	36.453477096349864	39.0	35.0	41.0	31.0	41.0
34	36.20736535966875	39.0	35.0	41.0	30.0	41.0
35	36.02739141995477	39.0	35.0	41.0	30.0	41.0
36	35.967975042996166	39.0	35.0	41.0	30.0	41.0
37	35.90074595604487	39.0	35.0	41.0	30.0	41.0
38	35.765083186566756	38.0	35.0	41.0	29.0	41.0
39	35.73111880173961	38.0	35.0	41.0	29.0	41.0
40	35.62471658095002	38.0	35.0	41.0	28.0	41.0
41	35.57313028199446	38.0	35.0	41.0	28.0	41.0
42	35.4684975306411	38.0	35.0	41.0	28.0	41.0
43	35.34826044902923	38.0	35.0	40.0	28.0	41.0
44	35.159813401404904	37.0	35.0	40.0	27.0	41.0
45	35.05851448099216	37.0	35.0	40.0	26.0	41.0
46	35.0122308010085	37.0	35.0	40.0	26.0	41.0
47	34.90484480648935	37.0	35.0	40.0	26.0	41.0
48	34.79788929051264	36.0	35.0	40.0	25.0	41.0
49	34.726477407733476	36.0	35.0	40.0	26.0	41.0
50	34.58732780341397	36.0	35.0	40.0	26.0	41.0
51	34.398482945647636	35.0	34.0	40.0	24.0	41.0
52	33.93634541499	35.0	34.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	2.0
11	3.0
12	3.0
13	9.0
14	24.0
15	35.0
16	116.0
17	211.0
18	498.0
19	1008.0
20	1825.0
21	2934.0
22	4619.0
23	7408.0
24	12744.0
25	23370.0
26	35169.0
27	43268.0
28	45647.0
29	44032.0
30	44928.0
31	50200.0
32	57551.0
33	73418.0
34	161008.0
35	222033.0
36	230774.0
37	220307.0
38	340537.0
39	792634.0
40	1923.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.535873005108265	16.613328955491994	21.54501684903767	32.305781190362076
2	37.38203709393488	19.105431679829827	22.24378979910588	21.268741427129413
3	25.764574965501758	20.30882803560773	34.58963320002696	19.336963798863554
4	22.347460279980595	22.73232711903166	32.96940459565825	21.9508080053295
5	20.77491099928502	31.104659216892955	29.780058960259925	18.340370823562104
6	76.30771813704105	1.960807017007004	18.756872252908003	2.9746025930439464
7	75.9328585801486	1.859700385280363	17.43537342669604	4.772067607874987
8	66.83656991720008	3.44957632392828	22.447119577510744	7.266734181360899
9	38.360683125199785	17.64850372523146	26.840440502365563	17.15037264720319
10	26.39317288324272	15.948961206894769	37.50154554615983	20.15632036370268
11	23.996097987006245	12.759615571496449	41.24927271456626	21.99501372693104
12	20.3393874633566	13.91082519138927	43.32565970526486	22.424127639989262
13	16.418848591888562	17.57659189186842	44.19492862368029	21.80963089256273
14	17.036240007708088	17.968819459118805	43.14631432211622	21.84862621105689
15	20.368499556908972	14.326210105783588	43.548507819119614	21.756782518187823
16	18.761875893987316	18.72536171983001	40.43165295076293	22.08110943541974
17	19.439931288842832	20.474072248441942	39.23421134139347	20.851785121321758
18	20.059721144187982	25.945243625630056	35.665333327268314	18.32970190291365
19	21.184630634110192	21.15555989296343	34.89324256204618	22.76656691088019
20	20.890780439815913	25.13361996064078	35.38529483644916	18.59030476309414
21	18.20328759895114	19.937442080786887	36.53861342902832	25.32065689123366
22	19.481862628135598	22.17766730252882	34.69173228948834	23.648737779847238
23	20.45930943963769	20.211525825197594	37.5411611507382	21.78800358442652
24	23.15895988775303	18.585921408099036	34.58376115842975	23.671357545718188
25	18.116488899567	19.045098520038756	37.24954398634709	25.588868594047153
26	17.949756000130673	18.390696701194546	37.39841264655809	26.261134652116684
27	21.517641556521088	20.814981480325145	36.44160068545747	21.22577627769629
28	22.5932176265456	22.060970813885643	32.64429198271966	22.701519576849105
29	23.32862880798796	18.842099560878804	35.511130206733085	22.318141424400153
30	25.111909947693338	21.37199838394799	30.88545011473225	22.630641553626422
31	26.155851427423016	20.114802548466056	29.47665636026877	24.25268966384216
32	24.864829324148687	23.2038272478444	28.484405387556812	23.446938040450096
33	20.332812430863946	23.37386833972986	31.46686493766745	24.826454291738738
34	19.16497914391423	27.48289147598728	29.56639108045152	23.785738299646976
35	20.38020228769778	28.795044658530443	28.045532306773644	22.779220746998124
36	20.686913080138066	29.203275606753508	26.404255327947318	23.7055559851611
37	19.47015989734679	31.192781193256746	28.06132892571826	21.275729983678204
38	18.33995729950596	28.864268585528563	26.98575285569375	25.810021259271725
39	20.700187202340214	25.66181423755055	32.12598093075167	21.512017629357562
40	17.67786393321752	24.388945840340842	34.73866726986042	23.194522956581213
41	19.739364057895024	26.12843478250082	29.77952137898694	24.35267978061722
42	18.872080054949077	21.077858722814412	32.28742072226939	27.762640499967123
43	21.710385119088727	21.136124262324774	33.46422748123738	23.68926313734912
44	22.19280228298361	21.779733103303688	33.05091018712377	22.976554426588937
45	22.10348108685701	22.493268862176155	32.06932813506027	23.333921915906576
46	21.892542465819133	21.93604519652524	31.44577521080423	24.7256371268514
47	19.141449625119765	23.467283424012265	33.6291822272323	23.76208472363567
48	21.084929984174433	21.20849097214957	30.689811883771622	27.016767159904376
49	20.12600905038749	18.60759006864086	35.185107840870984	26.081293040100668
50	19.3449861655527	18.809679274877297	36.83267038535066	25.01266417421934
51	18.4317182875638	17.97006003128723	35.260865447956135	28.337356233192835
52	15.872252494480488	19.47206210800504	38.765853995407404	25.88983140210707
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	708.0
1	3786.5
2	6865.0
3	87517.5
4	168170.0
5	95620.5
6	23071.0
7	20829.0
8	18587.0
9	17886.0
10	17185.0
11	16846.0
12	16507.0
13	15635.5
14	13932.5
15	13101.0
16	12224.0
17	11347.0
18	10600.5
19	9854.0
20	9505.0
21	9156.0
22	8721.0
23	8286.0
24	8332.0
25	8378.0
26	9439.0
27	10500.0
28	10930.5
29	11361.0
30	12859.0
31	14357.0
32	16196.0
33	18035.0
34	21664.0
35	25293.0
36	26870.0
37	28447.0
38	31215.0
39	38788.0
40	43593.0
41	54231.5
42	64870.0
43	87479.0
44	110088.0
45	146870.5
46	183653.0
47	205431.5
48	227210.0
49	227302.0
50	227394.0
51	204998.0
52	182602.0
53	156775.0
54	130948.0
55	124902.5
56	118857.0
57	114013.5
58	109170.0
59	108367.0
60	107564.0
61	105702.0
62	103840.0
63	106012.5
64	91066.5
65	73948.0
66	61941.5
67	49935.0
68	41273.5
69	32612.0
70	28118.0
71	23624.0
72	23662.0
73	23700.0
74	18830.5
75	13961.0
76	10361.5
77	6762.0
78	5402.0
79	4042.0
80	3008.0
81	1974.0
82	1491.5
83	1009.0
84	714.0
85	419.0
86	318.5
87	218.0
88	131.5
89	35.5
90	26.0
91	25.5
92	25.0
93	16.5
94	8.0
95	5.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	2418239.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.76809832531568
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.27827162160516	22.85401164688053
2	9.843154302891234	6.057102788452233
3	3.671998849632889	3.389412649678524
4	1.9229811440925795	2.3666589167667413
5	1.3712066400529743	2.109471036273783
6	0.981386718023884	1.8117241821190633
7	0.8262958950560553	1.7796487341422609
8	0.6795076725754169	1.6725727106085473
9	0.6061460097028212	1.6784964023430735
>10	5.435808740916242	31.622780895280933
>50	0.2335162671156447	4.940242075715931
>100	0.1377224789304765	7.452104224377841
>500	0.00687850145659446	1.4860336140324952
>1k	0.004046177327408506	2.6533231847982046
>5k	6.743628879014176E-4	1.4588111049343295
>10k+	4.046177327408506E-4	6.667605833595563
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	126136	5.216027034548695	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	19977	0.826097006954234	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA	14561	0.6021323781479002	No Hit
CCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTCTGCT	9792	0.40492275577393305	No Hit
CTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTCTGCTT	6985	0.28884655321496344	Illumina Single End Adapter 2 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTCTGC	6777	0.28024525284721646	No Hit
CGTTCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTCT	5883	0.24327620222815033	No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	5717	0.23641170289619845	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTC	4981	0.20597633236417076	No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	4759	0.1967960983178255	No Hit
GCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTCTGCT	4070	0.16830429084966372	No Hit
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	3419	0.14138387479484038	No Hit
GGGCCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTCT	3401	0.14063953149378536	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCG	3160	0.13067360174077086	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTT	3105	0.12839921943199165	No Hit
TGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	2780	0.11495968760738702	No Hit
CGTCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTCTG	2756	0.113967229872647	No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAA	2532	0.10470429101507336	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.29864707334552126	0.0
2	0.0	0.0	0.0	0.9980403094979445	0.0
3	0.0	0.0	0.0	1.6872608538692826	0.0
4	0.0	0.0	0.0	2.1354795783212497	0.0
5	0.0	0.0	0.0	4.422350313595968	0.0
6	0.0	0.0	0.0	5.442472807691878	0.0
7	0.0	0.0	0.0	8.038907651394258	0.0
8	0.0	0.0	0.0	11.003668371902032	0.0
9	0.0	0.0	0.0	13.931005165328985	0.0
10	4.1352405614168E-5	0.0	0.0	15.54606471899593	0.0
11	4.1352405614168E-5	0.0	0.0	16.790565365954315	0.0
12	4.1352405614168E-5	0.0	0.0	17.64474065632057	0.0
13	4.1352405614168E-5	0.0	0.0	18.116943776028755	0.0
14	4.1352405614168E-5	0.0	0.0	18.412696180981285	0.0
15	8.2704811228336E-5	0.0	0.0	19.08810502187749	0.0
16	8.2704811228336E-5	0.0	0.0	19.817106580449657	0.0
17	1.2405721684250398E-4	0.0	0.0	20.786903196913126	0.0
18	1.2405721684250398E-4	0.0	0.0	21.316420750802546	0.0
19	1.65409622456672E-4	0.0	0.0	21.893989800015632	0.0
20	1.65409622456672E-4	0.0	0.0	22.534952087035236	0.0
21	1.65409622456672E-4	0.0	0.0	23.207714373972134	0.0
22	2.0676202807084E-4	0.0	0.0	23.845244411325762	0.0
23	2.0676202807084E-4	0.0	0.0	24.433027504725548	0.0
24	2.4811443368500795E-4	0.0	0.0	24.901839727173368	0.0
25	2.4811443368500795E-4	0.0	0.0	25.354152339781137	0.0
26	2.4811443368500795E-4	0.0	0.0	25.82552841137704	0.0
27	2.8946683929917594E-4	0.0	0.0	26.339910984811674	0.0
28	3.30819244913344E-4	0.0	0.0	26.813850905555654	0.0
29	3.30819244913344E-4	0.0	0.0	27.31413230867586	0.0
30	3.72171650527512E-4	0.0	0.0	27.7666516833117	0.0
31	3.72171650527512E-4	0.0	0.0	28.23099784595319	0.0
32	3.72171650527512E-4	0.0	0.0	28.762004086444723	0.0
33	3.72171650527512E-4	0.0	0.0	29.252857141084895	0.0
34	3.72171650527512E-4	0.0	0.0	29.823727100588485	0.0
35	3.72171650527512E-4	0.0	0.0	30.292828789875607	0.0
36	3.72171650527512E-4	0.0	0.0	30.789347124084923	0.0
37	3.72171650527512E-4	0.0	0.0	31.412900048340962	0.0
38	3.72171650527512E-4	0.0	0.0	32.03107715986716	0.0
39	3.72171650527512E-4	0.0	0.0	32.7348951034203	0.0
40	4.1352405614168E-4	0.0	0.0	33.36994399643708	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	31825	0.0	45.31343	1
CACTTAC	5245	0.0	42.141087	36
CGGTCTA	595	0.0	42.134453	30
CTTACTG	5185	0.0	41.963356	38
TCACGAC	620	0.0	41.919353	24
CGACGGT	600	0.0	41.783333	27
ACGGGTA	215	0.0	41.72093	4
ACTTACT	5265	0.0	41.63153	37
GCACTTA	5305	0.0	41.577755	35
TCGGCGT	2165	0.0	41.53811	4
ACCCGCA	5405	0.0	41.531914	31
CCGCACT	5330	0.0	41.46904	33
CACGACG	605	0.0	41.43802	25
ACTACGG	100	0.0	41.399998	1
CGCACTT	5335	0.0	41.343956	34
TACGGGA	390	0.0	41.28205	3
CCCGCAC	5490	0.0	40.930786	32
TCGACGT	45	1.5896148E-8	40.88889	25
GTTTTTT	35905	0.0	40.67679	2
TTACTGG	5235	0.0	40.508118	39
>>END_MODULE