##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043353_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2378453
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.31689337565216	33.0	31.0	34.0	31.0	34.0
2	32.61111697393221	34.0	31.0	34.0	31.0	34.0
3	32.706991056791956	34.0	31.0	34.0	31.0	34.0
4	36.18429416095252	37.0	37.0	37.0	35.0	37.0
5	36.1808726092128	37.0	37.0	37.0	35.0	37.0
6	36.32822258837992	37.0	37.0	37.0	35.0	37.0
7	36.28320551215433	37.0	37.0	37.0	35.0	37.0
8	36.194642063559805	37.0	37.0	37.0	35.0	37.0
9	37.79531233116652	39.0	38.0	39.0	35.0	39.0
10	37.60558943144977	39.0	37.0	39.0	35.0	39.0
11	37.36242339032977	39.0	37.0	39.0	35.0	39.0
12	37.385166324497476	39.0	37.0	39.0	35.0	39.0
13	37.387239941255935	39.0	37.0	39.0	35.0	39.0
14	38.732526982875	40.0	38.0	41.0	35.0	41.0
15	38.78986172945187	41.0	38.0	41.0	35.0	41.0
16	38.68081816205744	41.0	38.0	41.0	34.0	41.0
17	38.64439995240604	40.0	38.0	41.0	34.0	41.0
18	38.442865173287004	40.0	38.0	41.0	34.0	41.0
19	38.19590254673941	40.0	37.0	41.0	34.0	41.0
20	37.902851979837315	40.0	35.0	41.0	34.0	41.0
21	37.842990801163616	40.0	35.0	41.0	34.0	41.0
22	37.80971497019281	40.0	35.0	41.0	34.0	41.0
23	37.790472630739394	40.0	35.0	41.0	34.0	41.0
24	37.70520922633325	40.0	35.0	41.0	34.0	41.0
25	37.670109100326975	39.0	35.0	41.0	34.0	41.0
26	37.637315515589336	39.0	35.0	41.0	34.0	41.0
27	37.61037909935576	39.0	35.0	41.0	34.0	41.0
28	37.51948682610083	39.0	35.0	41.0	34.0	41.0
29	37.466588997133854	39.0	35.0	41.0	34.0	41.0
30	37.336326175038984	39.0	35.0	41.0	33.0	41.0
31	37.20821222870496	39.0	35.0	41.0	33.0	41.0
32	36.933561016341294	39.0	35.0	41.0	33.0	41.0
33	36.5292549400808	39.0	35.0	41.0	31.0	41.0
34	36.23354382028991	39.0	35.0	41.0	31.0	41.0
35	36.05254718087765	39.0	35.0	41.0	30.0	41.0
36	35.976093704605475	39.0	35.0	41.0	30.0	41.0
37	35.851312176444104	39.0	35.0	41.0	30.0	41.0
38	35.72102412786799	38.0	35.0	41.0	29.0	41.0
39	35.643119708482786	38.0	35.0	41.0	29.0	41.0
40	35.51590887017738	38.0	35.0	41.0	28.0	41.0
41	35.425998327484294	38.0	35.0	41.0	28.0	41.0
42	35.299623326590854	38.0	35.0	40.0	27.0	41.0
43	35.171931503376356	37.0	35.0	40.0	27.0	41.0
44	35.03732215856273	37.0	35.0	40.0	26.0	41.0
45	34.96691042454906	37.0	35.0	40.0	26.0	41.0
46	34.86734150306943	37.0	35.0	40.0	25.0	41.0
47	34.708223370400844	36.0	35.0	40.0	24.0	41.0
48	34.63135155498133	36.0	35.0	40.0	24.0	41.0
49	34.544618287601224	36.0	34.0	40.0	25.0	41.0
50	34.44168457396467	36.0	34.0	40.0	24.0	41.0
51	33.98698229479414	35.0	34.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	4.0
11	2.0
12	3.0
13	8.0
14	21.0
15	25.0
16	86.0
17	223.0
18	470.0
19	923.0
20	1670.0
21	2666.0
22	4178.0
23	6504.0
24	11077.0
25	19782.0
26	32559.0
27	42125.0
28	45269.0
29	44418.0
30	44754.0
31	48887.0
32	55686.0
33	71057.0
34	156113.0
35	221336.0
36	220947.0
37	217802.0
38	354287.0
39	775364.0
40	207.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.82362905636563	16.724274139535236	21.63444053760995	31.817656266489188
2	37.0679176759011	19.179525515114236	22.352133929070703	21.40042287991396
3	25.84389937492984	20.390606835619625	34.256089987903906	19.509403801546636
4	22.509084686558868	22.847539976615053	32.55384907753064	22.089526259295432
5	20.93469999196957	31.22378285381296	29.449730560158223	18.391786594059248
6	76.66045955080887	1.9320541545281744	18.431476257886953	2.9760100367760054
7	76.27163538653066	1.8185770330546789	17.09968622461743	4.810101355797235
8	67.15461688753152	3.384006326801497	22.201195482946268	7.260181302720718
9	38.549679140180615	17.654458591361696	26.502857109221832	17.293005159235854
10	26.66859509101084	15.903026042557913	37.20792464681875	20.220454219612495
11	24.20892908121371	12.73760717575668	41.053323315617334	22.00014042741227
12	20.561011716439214	13.90315469761227	43.144556566810444	22.39127701913807
13	16.510059269617688	17.54556428064797	44.05094403799444	21.893432411739898
14	17.198994472457517	17.911474391127342	43.01363112914151	21.875900007273636
15	20.522835641486296	14.280837166006643	43.43024646692619	21.76608072558087
16	18.899805882226808	18.635011917410182	40.334620864906725	22.13056133545628
17	19.606988239834884	20.294704162747802	39.135690299535035	20.96261729788228
18	20.166932035234666	26.045458960088762	35.5359134698058	18.251695534870777
19	21.29215923123139	21.19608838181793	34.60131438376121	22.91043800318947
20	20.937390816635855	25.339748147220064	35.03121566833568	18.691645367808405
21	18.20792758990823	20.032264669514177	36.33517248396332	25.42463525661428
22	19.530257692710347	22.29196877129798	34.3299194896851	23.84785404630657
23	20.54474063603527	20.290331572665092	37.21868794548389	21.946239845815747
24	23.156942769102436	18.551638396890752	34.38941194129125	23.902006892715562
25	17.991904822168024	19.141811925650835	37.20632696967314	25.659956282508002
26	18.02373223267393	18.270489263399362	37.254593637124636	26.451184866802073
27	21.55039431092395	20.857591047626336	36.21778525789662	21.374229383553093
28	22.614783642981383	22.0862468167334	32.38285557881531	22.916113961469915
29	23.450158569456704	18.874369180303333	35.18101892280402	22.49445332743594
30	25.18847334801234	21.348330196140097	30.630792367980362	22.832404087867197
31	26.3955184315183	20.082633543736204	29.11943183237171	24.40241619237378
32	25.039763241064676	23.214711411156745	28.148422525061456	23.59710282271712
33	20.47192019350393	23.315070762382103	31.22714638464582	24.98586265946815
34	19.27618498242345	27.673576059732945	29.198516851079255	23.851722106764356
35	20.390606835619625	28.91614843766095	27.707169323926102	22.98607540279333
36	20.643123912896325	29.375522661158328	26.096458496341953	23.8848949296034
37	19.46963004944811	31.2580908683081	27.87475724767317	21.39752183457062
38	18.377827941102893	29.0424069762993	26.77193957585035	25.80782550674745
39	20.73763912929959	25.789788572656256	31.909018172736648	21.563554125307498
40	17.710251159051705	24.539732338625146	34.46572204706168	23.284294455261467
41	19.869595909610155	26.14073097092942	29.52873149059494	24.460941628865484
42	18.9908734795264	21.041786404860638	32.109526654510304	27.85781346110266
43	21.891960867000527	21.046327171484993	33.251066975046385	23.810644986468095
44	22.214018944246533	21.70108049223592	32.98908996730228	23.095810596215273
45	22.200480732644287	22.45245123616065	31.94824535107484	23.39882268012023
46	22.040082356052444	21.912226140268483	31.327842088954462	24.719849414724614
47	19.379823776210838	23.237078891195242	33.541171509380256	23.84192582321366
48	21.132643781483175	21.158080483406653	30.502095269488194	27.20718046562198
49	20.21107837741591	18.527210754217133	35.082971999026256	26.1787388693407
50	19.454115763481557	18.788220746846797	36.751493512800124	25.006169976871522
51	18.502741067408103	17.99955685481277	35.01784563327507	28.479856444504055
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	642.0
1	3657.0
2	6672.0
3	83576.5
4	160481.0
5	91906.0
6	23331.0
7	20894.0
8	18457.0
9	17709.0
10	16961.0
11	16417.5
12	15874.0
13	15090.5
14	14307.0
15	13483.0
16	12659.0
17	11670.5
18	10682.0
19	9939.0
20	9196.0
21	8728.0
22	8260.0
23	8083.0
24	7906.0
25	7862.5
26	9007.5
27	10196.0
28	10989.5
29	11783.0
30	13500.5
31	15218.0
32	17257.5
33	19297.0
34	23009.0
35	26721.0
36	28463.5
37	30206.0
38	33690.0
39	37174.0
40	43485.0
41	49796.0
42	61371.5
43	72947.0
44	101758.5
45	130570.0
46	169047.0
47	207524.0
48	235590.5
49	263657.0
50	238836.5
51	214016.0
52	179383.5
53	144751.0
54	132376.0
55	120001.0
56	117155.0
57	114309.0
58	111790.5
59	109272.0
60	107633.5
61	105995.0
62	102968.5
63	99942.0
64	99080.5
65	98219.0
66	79183.0
67	60147.0
68	49399.0
69	38651.0
70	33013.5
71	27376.0
72	25292.5
73	23209.0
74	19881.0
75	12505.0
76	8457.0
77	6513.5
78	4570.0
79	3562.5
80	2555.0
81	1873.5
82	1192.0
83	855.0
84	518.0
85	377.0
86	236.0
87	155.5
88	75.0
89	50.0
90	25.0
91	29.5
92	34.0
93	21.0
94	8.0
95	6.5
96	5.0
97	3.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2378453.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.543178145359345
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.76252546372932	22.529419556902898
2	10.060574976861048	6.145638675260229
3	3.7095222995090666	3.3990180128426544
4	1.9752833696138217	2.4132572738272255
5	1.355938129897451	2.0707329927771627
6	1.0656600963411886	1.9529187698969834
7	0.8477616877312508	1.8125335377230267
8	0.7031994612369992	1.7182357133025905
9	0.6229930917217196	1.7125370085406206
>10	5.510474217476116	31.706189629536624
>50	0.23235254668661007	4.927447499550984
>100	0.1420833126187366	7.715909129102775
>500	0.006231078523242542	1.3562408168784885
>1k	0.004292520760455973	2.687063748920256
>5k	6.923420581380601E-4	1.4255584761919424
>10k+	4.154052348828361E-4	6.427299158745717
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	120534	5.067747817594041	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	19288	0.810947283801698	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG	12150	0.5108362452400783	No Hit
CCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTCTGC	9269	0.3897070911218342	No Hit
CTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTCTGCT	6707	0.2819900161996054	No Hit
CGCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTCTG	6706	0.2819479720641947	No Hit
CGTTCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTC	5637	0.23700279131014992	No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	5388	0.2265338015928841	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTT	4732	0.19895284876346098	No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	4664	0.19609384755553297	No Hit
GCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTCTGC	4100	0.17238095518389474	No Hit
GGGCCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTC	3569	0.15005551928080985	No Hit
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	3204	0.13470940985590213	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCT	3140	0.13201858518961695	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCC	3033	0.12751986270067142	No Hit
TGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	2687	0.11297259184856713	No Hit
CGTCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTCT	2660	0.11183740019247805	No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA	2475	0.10405923514149742	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.2925010500522819	0.0
2	0.0	0.0	0.0	0.9864815491413957	0.0
3	0.0	0.0	0.0	1.6861800506463656	0.0
4	0.0	0.0	0.0	2.1451338327896328	0.0
5	0.0	0.0	0.0	4.477448156427728	0.0
6	0.0	0.0	0.0	5.499793353074456	0.0
7	0.0	0.0	0.0	8.168124406914915	0.0
8	0.0	0.0	0.0	11.209344897712926	0.0
9	0.0	0.0	0.0	14.250102903021418	0.0
10	0.0	0.0	0.0	15.921357285596983	0.0
11	0.0	0.0	0.0	17.194537794103983	0.0
12	0.0	0.0	0.0	18.08225766916563	0.0
13	0.0	0.0	0.0	18.56580727052416	0.0
14	0.0	0.0	0.0	18.868062559991724	0.0
15	4.204413541070604E-5	0.0	0.0	19.568854209017374	0.0
16	4.204413541070604E-5	0.0	0.0	20.31538987736987	0.0
17	4.204413541070604E-5	0.0	0.0	21.309985944645533	0.0
18	4.204413541070604E-5	0.0	0.0	21.86353903146289	0.0
19	4.204413541070604E-5	0.0	0.0	22.465358785731734	0.0
20	4.204413541070604E-5	0.0	0.0	23.123391549044694	0.0
21	4.204413541070604E-5	0.0	0.0	23.817834533623326	0.0
22	1.6817654164282415E-4	0.0	0.0	24.481879608300016	0.0
23	1.6817654164282415E-4	0.0	0.0	25.08163919993374	0.0
24	2.522648124642362E-4	0.0	0.0	25.555602738418628	0.0
25	2.522648124642362E-4	0.0	0.0	26.020022258165287	0.0
26	2.522648124642362E-4	0.0	0.0	26.47763062797541	0.0
27	2.522648124642362E-4	0.0	0.0	26.998473377443236	0.0
28	2.522648124642362E-4	0.0	0.0	27.484251317978536	0.0
29	2.522648124642362E-4	0.0	0.0	27.980372115824867	0.0
30	2.522648124642362E-4	0.0	0.0	28.44445528248824	0.0
31	2.522648124642362E-4	0.0	0.0	28.920100586389555	0.0
32	2.522648124642362E-4	0.0	0.0	29.466716390864146	0.0
33	2.522648124642362E-4	0.0	0.0	29.954386317492926	0.0
34	2.522648124642362E-4	0.0	0.0	30.5256820294536	0.0
35	2.522648124642362E-4	0.0	0.0	31.004648820052363	0.0
36	2.9430894787494223E-4	0.0	0.0	31.504049060460726	0.0
37	2.9430894787494223E-4	0.0	0.0	32.14438124276578	0.0
38	2.9430894787494223E-4	0.0	0.0	32.7653310786465	0.0
39	2.9430894787494223E-4	0.0	0.0	33.47331227482738	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATAACGG	95	0.0	45.0	1
TATAACG	20	7.034811E-4	45.0	21
CGTTTTT	30170	0.0	44.366093	1
TCTACGG	150	0.0	42.0	1
CGGTCTA	595	0.0	41.97479	30
ACGGGAT	510	0.0	41.911766	4
ACGGGTA	200	0.0	41.625004	4
ATGACGG	195	0.0	41.53846	1
TCACGAC	625	0.0	41.399998	24
CGACGGT	605	0.0	41.280994	27
CACGACG	605	0.0	41.280994	25
AATACGG	120	0.0	41.250004	1
TCGGCGT	1820	0.0	40.92033	4
CACTTAC	4975	0.0	40.79397	36
ACTTACT	4930	0.0	40.709938	37
TGTACGG	260	0.0	40.673077	1
CTTACTG	4925	0.0	40.614216	38
ATTGCGG	550	0.0	40.5	1
CGCACTT	5065	0.0	40.380062	34
GCACTTA	5060	0.0	40.28656	35
>>END_MODULE