FastQCFastQC Report
Thu 26 May 2016
SRR1043352_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1043352_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2720378
Sequences flagged as poor quality0
Sequence length52
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT838063.0806748179848538No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC194020.7132097083567064No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA144710.5319481336784815No Hit
CCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCTGCT101720.3739186245440891Illumina Single End Adapter 2 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCTGCTT85100.3128241736993903Illumina Single End Adapter 2 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCTGCT74590.2741898368535549Illumina Single End Adapter 2 (95% over 21bp)
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC55190.2028762179373602No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC54170.19912673900465303No Hit
TGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC41600.15291992509864438No Hit
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC40780.14990563811352686No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAA36680.13483420318793932No Hit
CGCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCTGC35680.13115824344999114No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCG34120.125423746258792No Hit
TCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCTGCT29830.10965387898299428Illumina Single End Adapter 2 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAAGA206.3129835E-446.031
CGTTTTT231500.045.2151221
TCACGAC7400.044.44594224
TACGGGA3750.044.163
CGAATAT1200.044.08333214
CGGTCTA7100.043.73239530
ATAACGG1000.043.71
TATACGG1700.043.2941171
TCCGTAC1650.043.2121246
CTAACGG2250.042.9333341
CGACGGT7250.042.19310427
TACGCGG2350.042.0851061
CATGCGG2800.041.8928571
AGTAAGG3850.041.818181
TAGGGTA12900.041.1860434
TCGGCGT21250.041.021184
GCGATAT900.040.888898
AGGGTAC8850.040.8022585
CGCACTT58700.040.78875734
TCCCCCA21450.040.74592210