##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043352_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2720378
Sequences flagged as poor quality	0
Sequence length	52
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.28467771758189	33.0	31.0	34.0	31.0	34.0
2	32.62754808339135	34.0	31.0	34.0	31.0	34.0
3	32.78380908829582	34.0	31.0	34.0	31.0	34.0
4	36.19111461715982	37.0	37.0	37.0	35.0	37.0
5	36.13966956062723	37.0	37.0	37.0	35.0	37.0
6	36.287275518328705	37.0	37.0	37.0	35.0	37.0
7	36.23139504877631	37.0	37.0	37.0	35.0	37.0
8	36.21612437683292	37.0	37.0	37.0	35.0	37.0
9	37.77847968186774	39.0	38.0	39.0	35.0	39.0
10	37.60216374342095	39.0	37.0	39.0	35.0	39.0
11	37.22643434110995	39.0	37.0	39.0	35.0	39.0
12	37.220236305395794	39.0	37.0	39.0	34.0	39.0
13	37.212104347263505	39.0	37.0	39.0	34.0	39.0
14	38.52061662019028	40.0	37.0	41.0	35.0	41.0
15	38.5601868563854	40.0	37.0	41.0	35.0	41.0
16	38.37714170604232	40.0	37.0	41.0	34.0	41.0
17	38.40496835366262	40.0	37.0	41.0	34.0	41.0
18	38.26737350471148	40.0	37.0	41.0	34.0	41.0
19	38.06479393672497	40.0	36.0	41.0	34.0	41.0
20	37.85834395073038	40.0	35.0	41.0	34.0	41.0
21	37.776758965114404	40.0	35.0	41.0	34.0	41.0
22	37.73941452254062	40.0	35.0	41.0	34.0	41.0
23	37.75240904021427	40.0	35.0	41.0	34.0	41.0
24	37.659536652626954	40.0	35.0	41.0	34.0	41.0
25	37.54504888658855	39.0	35.0	41.0	34.0	41.0
26	37.449304839253955	39.0	35.0	41.0	33.0	41.0
27	37.48436687842646	39.0	35.0	41.0	34.0	41.0
28	37.48048396215526	39.0	35.0	41.0	34.0	41.0
29	37.40248450766769	39.0	35.0	41.0	33.0	41.0
30	37.36537312094128	39.0	35.0	41.0	33.0	41.0
31	37.274704838812845	39.0	35.0	41.0	33.0	41.0
32	37.0682386785954	39.0	35.0	41.0	33.0	41.0
33	36.758312263957436	39.0	35.0	41.0	32.0	41.0
34	36.601682927887225	39.0	35.0	41.0	31.0	41.0
35	36.474759390055354	39.0	35.0	41.0	31.0	41.0
36	36.41009153874939	39.0	35.0	41.0	31.0	41.0
37	36.38874744612697	39.0	35.0	41.0	31.0	41.0
38	36.27145823117228	39.0	35.0	41.0	31.0	41.0
39	36.23746552868756	39.0	35.0	41.0	31.0	41.0
40	36.166667279326624	38.0	35.0	41.0	31.0	41.0
41	36.13706587834485	38.0	35.0	41.0	31.0	41.0
42	36.05935719227254	38.0	35.0	41.0	31.0	41.0
43	35.96970715099152	38.0	35.0	41.0	31.0	41.0
44	35.78901020372904	38.0	35.0	41.0	30.0	41.0
45	35.71172609100647	38.0	35.0	40.0	30.0	41.0
46	35.6783259532315	37.0	35.0	40.0	30.0	41.0
47	35.59599107182899	37.0	35.0	40.0	30.0	41.0
48	35.50440894610969	37.0	35.0	40.0	30.0	41.0
49	35.48457309976775	37.0	35.0	40.0	30.0	41.0
50	35.389209146670055	37.0	35.0	40.0	30.0	41.0
51	35.27117334429259	36.0	35.0	40.0	29.0	41.0
52	34.886487466080084	36.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	3.0
12	5.0
13	8.0
14	19.0
15	50.0
16	93.0
17	216.0
18	487.0
19	951.0
20	1803.0
21	2929.0
22	4525.0
23	7264.0
24	12097.0
25	20489.0
26	30442.0
27	37942.0
28	40331.0
29	41798.0
30	46091.0
31	52732.0
32	63553.0
33	82787.0
34	193113.0
35	284656.0
36	225097.0
37	252041.0
38	379753.0
39	936528.0
40	2573.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.75055084256673	17.767383797398743	23.55915244131514	26.922912918719383
2	33.625327068517684	20.73660351612901	25.320157713376595	20.31791170197671
3	28.299376042594083	21.065895989454404	31.057264835989706	19.57746313196181
4	23.909912519510158	23.38682344880013	29.4656477886529	23.23761624303681
5	21.136842012396805	32.95747870332726	26.916590267970115	18.989089016305822
6	80.23965787107527	2.1920115513358804	14.76548479659812	2.80284578099073
7	80.28160057168526	2.2051347276003557	13.083659697292068	4.429605003422318
8	70.96712295129574	3.685958348435401	18.316351624663927	7.030567075604935
9	41.95130235577555	16.764508461691722	23.048708672103658	18.235480510429063
10	29.889632984827845	16.759031281682176	32.2097884926286	21.14154724086138
11	26.92177337120062	13.939202566702127	37.00063005949909	22.13839400259817
12	20.80615267437099	16.249028627639248	39.53479994324318	23.410018754746584
13	16.924927344655778	19.708731654203937	42.165132933732	21.201208067408277
14	19.333673482141084	19.077679645990372	39.57810274895621	22.010544122912332
15	22.545249226394272	14.652522553850972	40.814475047217705	21.987753172537055
16	19.43042474244388	19.43321847184472	36.6306447118746	24.505712073836797
17	22.212538110512583	20.660400870761343	34.956869964394656	22.17019105433142
18	21.69555113296755	26.620601989870522	32.256032066131986	19.427814811029936
19	24.145431259920496	20.97976825279428	30.959226989778628	23.915573497506596
20	22.64699979194068	26.41592455166157	32.11678671125851	18.82028894513924
21	19.249935119310624	20.086399757680734	35.24502844825241	25.41863667475623
22	22.28359441224712	21.698234583576255	31.43055854737834	24.587612456798283
23	23.467069649879537	20.18241582603594	34.48226680262817	21.868247721456356
24	24.44447058460258	18.142258171474698	31.507643423083113	25.905627820839605
25	18.55929580374492	20.845228126385376	34.72583589486461	25.86964017500509
26	17.74646758648982	20.137458838440832	34.3223993136248	27.79367426144455
27	21.327661082393696	23.0878576433128	34.13654278927414	21.44793848501936
28	23.177808378100394	22.271169668332856	30.862733046657485	23.68828890690926
29	22.63986843004906	21.111477890204963	33.43303761462562	22.815616065120363
30	27.033522547234245	19.64976926000725	30.954742318898333	22.361965873860175
31	28.30452238622721	21.139304905421234	28.11620296885212	22.43996973949944
32	24.847319012284323	20.578978362565792	29.40234776196543	25.171354863184455
33	21.14143696206924	20.881877444972723	33.87547612868506	24.10120946427298
34	21.86593186682145	21.651145539333136	32.70843243108127	23.774490162764145
35	24.90565649332556	24.053054391705857	27.915569086354914	23.12572002861367
36	23.818234083645727	21.381036017788706	30.11074196306543	24.68998793550014
37	23.34859346752547	24.252585486281685	30.107837954872448	22.290983091320395
38	23.824924330368795	20.143891767982243	28.877935345749744	27.153248555899218
39	24.130800940163464	18.418690343768404	33.26651663849656	24.183992077571574
40	18.646232251547396	20.09533233984395	36.28503097731271	24.973404431295947
41	21.04740591197253	23.46063672033813	29.40462685700296	26.087330510686385
42	20.70013799552856	17.39309757688086	32.81540285945556	29.091361568135017
43	22.956442082681157	19.402266890851198	32.221992678958586	25.419298347509063
44	22.570723627378253	20.311074416864127	31.83645802164258	25.281743934115035
45	22.755183287028494	20.3387176340935	31.28973252981755	25.61636654906046
46	23.303415922346087	19.490269366977678	31.808300170049897	25.398014540626342
47	20.017475512594203	21.03442977409757	34.96980934267223	23.978285370635994
48	21.63622114279707	19.61936907297442	31.323661638198807	27.420748146029705
49	20.820562436543746	17.096557904820582	35.886998056887684	26.195881601747995
50	20.090884428561033	17.463014331096634	36.64542207002115	25.80067917032118
51	19.410758357845857	16.202490977356824	34.552955508388905	29.83379515640841
52	17.718971407649967	17.593731459378073	38.879339562369644	25.80795757060232
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	857.0
1	4672.0
2	8487.0
3	64025.0
4	119563.0
5	68852.0
6	18141.0
7	16523.0
8	14905.0
9	14554.0
10	14203.0
11	13857.0
12	13511.0
13	12746.0
14	11189.5
15	10398.0
16	9822.0
17	9246.0
18	8514.5
19	7783.0
20	7535.5
21	7288.0
22	7293.0
23	7298.0
24	7541.5
25	7785.0
26	9264.5
27	10744.0
28	10760.5
29	10777.0
30	12347.5
31	13918.0
32	15540.0
33	17162.0
34	21473.5
35	25785.0
36	29613.0
37	33441.0
38	35275.0
39	41812.5
40	46516.0
41	56016.5
42	65517.0
43	84734.5
44	103952.0
45	129384.5
46	154817.0
47	193727.0
48	232637.0
49	263231.0
50	293825.0
51	276122.5
52	258420.0
53	229029.5
54	199639.0
55	185350.0
56	171061.0
57	160855.5
58	150650.0
59	145091.0
60	139532.0
61	136956.0
62	134380.0
63	130657.5
64	102787.0
65	78639.0
66	64039.0
67	49439.0
68	41652.0
69	33865.0
70	29009.5
71	24154.0
72	25079.0
73	26004.0
74	20393.0
75	14782.0
76	11219.5
77	7657.0
78	5884.5
79	4112.0
80	3062.5
81	2013.0
82	1460.0
83	907.0
84	611.0
85	315.0
86	238.5
87	162.0
88	98.5
89	25.0
90	15.0
91	11.0
92	7.0
93	4.5
94	2.0
95	3.5
96	5.0
97	3.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	2720378.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.56722671812089
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.47112170293632	18.27318372377383
2	11.489593675594	5.875140928059993
3	4.768417988539625	3.657456713992757
4	2.431012003015609	2.4861694014229303
5	1.4170976123966814	1.8115627968926875
6	0.9083667587469498	1.3934651318452733
7	0.6686466838980408	1.1966808953078645
8	0.48873795897836175	0.9996539362361154
9	0.37912311091349754	0.8723813877724215
>10	4.914914680965725	30.67176991147998
>50	0.8602503299191544	14.461053407277483
>100	0.186067631567985	8.29660021460188
>500	0.010279480343502134	1.7864577552587029
>1k	0.005212130878395448	2.489884056478759
>5k	5.791256531550498E-4	0.9959314126361949
>10k+	5.791256531550498E-4	4.732608326963395
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	83806	3.0806748179848538	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	19402	0.7132097083567064	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA	14471	0.5319481336784815	No Hit
CCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCTGCT	10172	0.3739186245440891	Illumina Single End Adapter 2 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCTGCTT	8510	0.3128241736993903	Illumina Single End Adapter 2 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCTGCT	7459	0.2741898368535549	Illumina Single End Adapter 2 (95% over 21bp)
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	5519	0.2028762179373602	No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	5417	0.19912673900465303	No Hit
TGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	4160	0.15291992509864438	No Hit
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	4078	0.14990563811352686	No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAA	3668	0.13483420318793932	No Hit
CGCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCTGC	3568	0.13115824344999114	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCG	3412	0.125423746258792	No Hit
TCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCTGCT	2983	0.10965387898299428	Illumina Single End Adapter 2 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.32282278418660937	0.0
2	0.0	0.0	0.0	1.1661982268640607	0.0
3	0.0	0.0	0.0	1.84514799046309	0.0
4	0.0	0.0	0.0	2.2929901653373173	0.0
5	0.0	0.0	0.0	4.768749048845418	0.0
6	0.0	0.0	0.0	5.331060609959351	0.0
7	0.0	0.0	0.0	7.342509018967217	0.0
8	0.0	0.0	0.0	9.659466441795956	0.0
9	0.0	0.0	0.0	11.594859243825674	0.0
10	0.0	0.0	0.0	12.898685403278515	0.0
11	0.0	0.0	0.0	14.107267445921119	0.0
12	0.0	0.0	0.0	15.018390826568956	0.0
13	0.0	0.0	0.0	15.455646237397891	0.0
14	0.0	0.0	0.0	15.737224753324721	0.0
15	0.0	0.0	0.0	16.613058920488257	0.0
16	0.0	0.0	0.0	17.581674311437602	3.6759597379481824E-5
17	0.0	0.0	0.0	18.776140668686484	3.6759597379481824E-5
18	0.0	0.0	0.0	19.483542360657232	3.6759597379481824E-5
19	0.0	0.0	0.0	20.23016654303189	3.6759597379481824E-5
20	3.6759597379481824E-5	0.0	0.0	21.051817063658063	3.6759597379481824E-5
21	3.6759597379481824E-5	0.0	0.0	21.839281158721324	3.6759597379481824E-5
22	1.8379798689740912E-4	0.0	0.0	22.6299433387566	3.6759597379481824E-5
23	2.2055758427689094E-4	0.0	0.0	23.336977434753553	3.6759597379481824E-5
24	2.940767790358546E-4	0.0	0.0	23.903516349566125	3.6759597379481824E-5
25	2.940767790358546E-4	0.0	0.0	24.449212572664536	3.6759597379481824E-5
26	2.940767790358546E-4	0.0	0.0	24.957818362007046	3.6759597379481824E-5
27	2.940767790358546E-4	0.0	0.0	25.56126391258862	3.6759597379481824E-5
28	2.940767790358546E-4	0.0	0.0	26.11802477449825	3.6759597379481824E-5
29	2.940767790358546E-4	0.0	0.0	26.707979552841554	3.6759597379481824E-5
30	2.940767790358546E-4	0.0	0.0	27.267497384554648	3.6759597379481824E-5
31	2.940767790358546E-4	0.0	0.0	27.82911051331837	3.6759597379481824E-5
32	2.940767790358546E-4	0.0	0.0	28.49019511259097	3.6759597379481824E-5
33	3.308363764153364E-4	0.0	0.0	29.060336467946733	3.6759597379481824E-5
34	3.308363764153364E-4	0.0	0.0	29.69609370462487	3.6759597379481824E-5
35	3.308363764153364E-4	0.0	0.0	30.237305256842983	3.6759597379481824E-5
36	3.308363764153364E-4	0.0	0.0	30.791235629754393	3.6759597379481824E-5
37	3.308363764153364E-4	0.0	0.0	31.54201364663293	3.6759597379481824E-5
38	3.6759597379481824E-4	0.0	0.0	32.20045155489421	3.6759597379481824E-5
39	4.0435557117430004E-4	0.0	0.0	33.07088206124296	3.6759597379481824E-5
40	4.0435557117430004E-4	0.0	0.0	33.818425233552105	3.6759597379481824E-5
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTAAGA	20	6.3129835E-4	46.0	31
CGTTTTT	23150	0.0	45.215122	1
TCACGAC	740	0.0	44.445942	24
TACGGGA	375	0.0	44.16	3
CGAATAT	120	0.0	44.083332	14
CGGTCTA	710	0.0	43.732395	30
ATAACGG	100	0.0	43.7	1
TATACGG	170	0.0	43.294117	1
TCCGTAC	165	0.0	43.21212	46
CTAACGG	225	0.0	42.933334	1
CGACGGT	725	0.0	42.193104	27
TACGCGG	235	0.0	42.085106	1
CATGCGG	280	0.0	41.892857	1
AGTAAGG	385	0.0	41.81818	1
TAGGGTA	1290	0.0	41.186043	4
TCGGCGT	2125	0.0	41.02118	4
GCGATAT	90	0.0	40.88889	8
AGGGTAC	885	0.0	40.802258	5
CGCACTT	5870	0.0	40.788757	34
TCCCCCA	2145	0.0	40.745922	10
>>END_MODULE