FastQCFastQC Report
Thu 26 May 2016
SRR1043351_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1043351_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2668991
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT800492.9992233019894035No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC185550.6952065405990504No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG118310.44327612944367367No Hit
CCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCTGC95310.357101241630264No Hit
CTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCTGCT85540.3204956479808287Illumina Single End Adapter 2 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCTGC77330.28973495976569424No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC53270.19958853364436224No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC53070.19883918679381085No Hit
TGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC40220.15069365164588416No Hit
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC38410.1439120626483941No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA36180.13555684526474612No Hit
CGCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCTG34750.1301990152833037No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCC31560.11824693301700905No Hit
TCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCTGC28260.10588270998291113No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG253.892197E-545.037
ATACTAC207.034965E-445.012
CGTACGT253.892197E-545.019
CGACATA302.1665855E-644.99999614
CGTTTTT221700.044.2286871
CGGTCTA4900.043.16326530
TATACGG1650.042.2727281
TAAACGG2350.041.170211
CATGCGG3950.041.0126571
ACCCGCA59050.040.69432431
CGAATAT1550.040.6451614
CGCACTT58350.040.56555634
CCGCACT58400.040.5308233
GCACTTA58850.040.29736735
CACTTAC58950.040.22900836
CCCGCAC60000.040.19999732
TCACGAC5550.040.13513624
TACGGGT1800.040.03
CTAGCGG7900.039.8734171
ATTGCGG5200.039.807691
Produced by FastQC (version 0.11.5)