##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043351_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2668991
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.31289802026309	33.0	31.0	34.0	31.0	34.0
2	32.59029798152186	34.0	31.0	34.0	31.0	34.0
3	32.717849179708736	34.0	31.0	34.0	31.0	34.0
4	36.17639512459952	37.0	37.0	37.0	35.0	37.0
5	36.165842822250056	37.0	37.0	37.0	35.0	37.0
6	36.31910373620593	37.0	37.0	37.0	35.0	37.0
7	36.27145127128566	37.0	37.0	37.0	35.0	37.0
8	36.17879865462267	37.0	37.0	37.0	35.0	37.0
9	37.74903100085388	39.0	38.0	39.0	35.0	39.0
10	37.557729494029765	39.0	37.0	39.0	35.0	39.0
11	37.225488583513396	39.0	37.0	39.0	35.0	39.0
12	37.22901276175154	39.0	37.0	39.0	35.0	39.0
13	37.23561488217832	39.0	37.0	39.0	35.0	39.0
14	38.5469666252153	40.0	37.0	41.0	35.0	41.0
15	38.596277394715834	40.0	37.0	41.0	35.0	41.0
16	38.459605521337465	40.0	37.0	41.0	34.0	41.0
17	38.454140160082964	40.0	37.0	41.0	34.0	41.0
18	38.335878614802375	40.0	37.0	41.0	34.0	41.0
19	38.153927457979435	40.0	37.0	41.0	34.0	41.0
20	37.966332220678154	40.0	35.0	41.0	34.0	41.0
21	37.885464581933775	40.0	35.0	41.0	34.0	41.0
22	37.8665799172796	40.0	35.0	41.0	34.0	41.0
23	37.84321490780599	40.0	35.0	41.0	34.0	41.0
24	37.754269684686086	40.0	35.0	41.0	34.0	41.0
25	37.71009681186636	40.0	35.0	41.0	34.0	41.0
26	37.677207603922234	40.0	35.0	41.0	34.0	41.0
27	37.660051682452284	40.0	35.0	41.0	34.0	41.0
28	37.574188148255274	39.0	35.0	41.0	34.0	41.0
29	37.54226597242179	39.0	35.0	41.0	34.0	41.0
30	37.462983951613175	40.0	35.0	41.0	34.0	41.0
31	37.35811248520508	40.0	35.0	41.0	33.0	41.0
32	37.168188277892284	39.0	35.0	41.0	33.0	41.0
33	36.84263866007791	39.0	35.0	41.0	32.0	41.0
34	36.645539081997654	39.0	35.0	41.0	32.0	41.0
35	36.51258883975255	39.0	35.0	41.0	31.0	41.0
36	36.43578003822418	39.0	35.0	41.0	31.0	41.0
37	36.35995475443716	39.0	35.0	41.0	31.0	41.0
38	36.2518240039026	39.0	35.0	41.0	31.0	41.0
39	36.17428046778726	38.0	35.0	41.0	31.0	41.0
40	36.079911472163076	38.0	35.0	41.0	31.0	41.0
41	36.00423718176644	38.0	35.0	41.0	31.0	41.0
42	35.90650436813013	38.0	35.0	41.0	30.0	41.0
43	35.80135864077474	38.0	35.0	40.0	30.0	41.0
44	35.70405445353693	38.0	35.0	40.0	30.0	41.0
45	35.65249414479105	38.0	35.0	40.0	30.0	41.0
46	35.556026228638466	37.0	35.0	40.0	30.0	41.0
47	35.41228127033774	37.0	35.0	40.0	29.0	41.0
48	35.34862238201627	37.0	35.0	40.0	29.0	41.0
49	35.30916477425364	37.0	35.0	40.0	29.0	41.0
50	35.24612859316498	36.0	35.0	40.0	29.0	41.0
51	34.83768135598809	36.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	0.0
12	1.0
13	9.0
14	20.0
15	45.0
16	97.0
17	197.0
18	436.0
19	890.0
20	1572.0
21	2680.0
22	4171.0
23	6488.0
24	10520.0
25	17815.0
26	28306.0
27	36297.0
28	40211.0
29	41719.0
30	44872.0
31	51091.0
32	60189.0
33	79536.0
34	187280.0
35	281747.0
36	215090.0
37	247485.0
38	395624.0
39	914334.0
40	267.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.003292630061324	17.794889529413922	23.620461814970525	26.581356025554225
2	33.38418900625742	20.7594180722228	25.39768774042325	20.458705181096526
3	28.281024551974888	21.117905605526584	30.95731682871917	19.643753013779364
4	24.006000769579217	23.530352856191723	29.167464408834647	23.296181965394414
5	21.31423448037105	33.00003634332225	26.704173974359595	18.981555201947103
6	80.42668559017247	2.175091635752987	14.585886576612662	2.812336197461887
7	80.49985931012881	2.171757042268033	12.87992353664737	4.448460110955788
8	71.11897342478862	3.6520917455323003	18.181402634928332	7.047532194750751
9	42.09047538938873	16.751274170651005	22.827203238976825	18.331047200983445
10	30.137418972188367	16.828194624860107	31.94173378628853	21.092652616663003
11	27.125456773739586	13.933467741180095	36.89240615648385	22.048669328596464
12	20.987069645420313	16.234711919223408	39.35292400761187	23.42529442774442
13	17.014707055962347	19.66301122783854	42.06866939603768	21.253612320161437
14	19.394183045203224	19.114564267919974	39.499046643469384	21.992206043407414
15	22.692920283358017	14.65433941141053	40.69410500072874	21.958635304502714
16	19.48935009522325	19.39751763868818	36.62159220469458	24.491540061393987
17	22.331510297337083	20.606851053450537	34.8128937115187	22.248744937693683
18	21.79591463590548	26.761686345139417	32.13221775569869	19.310181263256414
19	24.271194619989352	21.070509417229207	30.707109915320057	23.95118604746138
20	22.686775639183494	26.62627936924478	31.783321862081966	18.90362312948976
21	19.27807175071029	20.1717802720204	35.11877709591377	25.431370881355537
22	22.35267934586516	21.741249783157755	31.166721806105752	24.739349064871334
23	23.54533979320275	20.241844202546954	34.279133949870946	21.933682054379354
24	24.516867984942625	18.081439765064776	31.416591513422116	25.985100736570487
25	18.42126856178983	20.880475055929377	34.73975745890488	25.95849892337591
26	17.738089038142128	20.06867014538453	34.25103344297526	27.942207373498075
27	21.317306802458308	23.094232989170816	34.024056281943245	21.564403926427627
28	23.129527225831783	22.349045013639987	30.72108523408284	23.800342526445387
29	22.674411416149397	21.208089498990443	33.2185084176005	22.89899066725965
30	27.15655466803747	19.554655673248806	30.81377943949605	22.475010219217676
31	28.4390992700987	21.16972294024221	27.89705922575235	22.494118563906735
32	24.8890685656115	20.520900969692292	29.235167896781967	25.354862567914243
33	21.206291066549117	20.852412016376228	33.74994520401155	24.1913517130631
34	21.937316386604525	21.618956377147768	32.6361535126945	23.807573723553208
35	25.051114822043235	24.060403350929246	27.659141600702284	23.229340226325228
36	23.778986141204673	21.32064139594326	30.068404127252585	24.831968335599484
37	23.383293536771014	24.125371722872053	30.09571781995518	22.395616920401753
38	23.949462549705114	20.031502541597177	28.84202307164018	27.177011837057524
39	24.14773972636101	18.347345494982935	33.251404744339716	24.25351003431634
40	18.74554841136594	19.965822290146352	36.30045961189079	24.98816968659692
41	21.14488209214643	23.349198255070924	29.270237329387772	26.235682323394872
42	20.83371581245497	17.34483181097276	32.80988957999484	29.011562796577433
43	23.048597765972232	19.23674527190238	32.187144879844105	25.527512082281284
44	22.575834837959363	20.18579305812571	31.8528237824706	25.385548321444322
45	22.8063713965315	20.186392535606153	31.343380326123242	25.663855741739106
46	23.44001909335775	19.448173485785453	31.742257654671747	25.369549766185052
47	20.160952210029933	20.783022498015168	35.012444777820534	24.04358051413437
48	21.693029313324775	19.492272547940402	31.33524991279476	27.479448225940068
49	20.92082738383157	16.956108132249227	35.91323462686836	26.20982985705085
50	20.107561246928146	17.35843245631027	36.75003025487909	25.783976041882497
51	19.409694525009638	16.20814757337136	34.44739978516226	29.934758116456745
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	763.0
1	4407.0
2	8051.0
3	61099.0
4	114147.0
5	66316.5
6	18486.0
7	16672.0
8	14858.0
9	14351.5
10	13845.0
11	13405.0
12	12965.0
13	12263.5
14	11562.0
15	10759.0
16	9956.0
17	9193.0
18	8430.0
19	7771.5
20	7113.0
21	7070.5
22	7028.0
23	6729.5
24	6431.0
25	7153.5
26	9101.5
27	10327.0
28	11248.0
29	12169.0
30	13453.5
31	14738.0
32	16802.5
33	18867.0
34	23098.5
35	27330.0
36	31473.0
37	35616.0
38	37181.5
39	38747.0
40	46567.5
41	54388.0
42	67115.5
43	79843.0
44	95704.0
45	111565.0
46	143232.0
47	174899.0
48	227032.5
49	279166.0
50	291985.5
51	304805.0
52	265310.5
53	225816.0
54	202587.0
55	179358.0
56	168485.0
57	157612.0
58	151269.5
59	144927.0
60	140822.5
61	136718.0
62	130116.5
63	123515.0
64	115730.0
65	107945.0
66	84179.5
67	60414.0
68	50124.0
69	39834.0
70	34238.5
71	28643.0
72	26811.5
73	24980.0
74	20952.5
75	13143.5
76	9362.0
77	7102.5
78	4843.0
79	3657.0
80	2471.0
81	1747.5
82	1024.0
83	707.0
84	390.0
85	284.0
86	178.0
87	106.5
88	35.0
89	25.0
90	15.0
91	11.0
92	7.0
93	6.0
94	5.0
95	3.5
96	2.0
97	1.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2668991.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.203471311566737
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.67040769540115	17.81139592927768
2	11.759442781156487	5.927575575497762
3	4.856528461967484	3.672041272950145
4	2.53422017716374	2.5548458212935965
5	1.4865553317279487	1.8733177328130968
6	0.943014106055007	1.4260337381016075
7	0.6504681734385753	1.1475839163842447
8	0.5042863389455786	1.016781302114393
9	0.4116386394080663	0.9337250375148207
>10	5.138652261666316	31.56055328463887
>50	0.8383895221844689	13.851437694478177
>100	0.18967535442901948	8.388998357434728
>500	0.010092950293426153	1.7539890071136666
>1k	0.005423077769602112	2.5048958555203957
>5k	7.532052457780711E-4	1.3839648315361568
>10k+	4.519231474668427E-4	4.192860643330832
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	80049	2.9992233019894035	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	18555	0.6952065405990504	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG	11831	0.44327612944367367	No Hit
CCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCTGC	9531	0.357101241630264	No Hit
CTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCTGCT	8554	0.3204956479808287	Illumina Single End Adapter 2 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCTGC	7733	0.28973495976569424	No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	5327	0.19958853364436224	No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	5307	0.19883918679381085	No Hit
TGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	4022	0.15069365164588416	No Hit
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	3841	0.1439120626483941	No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA	3618	0.13555684526474612	No Hit
CGCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCTG	3475	0.1301990152833037	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCC	3156	0.11824693301700905	No Hit
TCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCTGC	2826	0.10588270998291113	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.3293379408173351	0.0
2	0.0	0.0	0.0	1.1703673785336857	0.0
3	0.0	0.0	0.0	1.8614525114547034	0.0
4	0.0	0.0	0.0	2.323349910134579	0.0
5	0.0	0.0	0.0	4.85336968165123	0.0
6	0.0	0.0	0.0	5.431378374824044	0.0
7	0.0	0.0	0.0	7.472149587615695	0.0
8	0.0	0.0	0.0	9.871895409163987	0.0
9	0.0	0.0	0.0	11.872838836848832	0.0
10	0.0	0.0	0.0	13.217766564218463	0.0
11	0.0	0.0	0.0	14.462057009559043	0.0
12	0.0	0.0	0.0	15.398852974775862	0.0
13	0.0	0.0	0.0	15.85576721689957	0.0
14	0.0	0.0	0.0	16.15374499202133	0.0
15	0.0	0.0	0.0	17.054459906384096	0.0
16	0.0	0.0	0.0	18.04494657344292	3.746734252756941E-5
17	0.0	0.0	0.0	19.267168754034763	3.746734252756941E-5
18	0.0	0.0	0.0	20.00512553245777	3.746734252756941E-5
19	0.0	0.0	0.0	20.763352143188193	3.746734252756941E-5
20	0.0	0.0	0.0	21.605805339920593	3.746734252756941E-5
21	0.0	0.0	0.0	22.40846821888871	3.746734252756941E-5
22	1.1240202758270822E-4	0.0	0.0	23.218774435732456	3.746734252756941E-5
23	1.8733671263784703E-4	0.0	0.0	23.93563710031244	3.746734252756941E-5
24	2.997387402205553E-4	0.0	0.0	24.51757986445065	3.746734252756941E-5
25	2.997387402205553E-4	0.0	0.0	25.069511287224273	3.746734252756941E-5
26	2.997387402205553E-4	0.0	0.0	25.588021840463306	3.746734252756941E-5
27	2.997387402205553E-4	0.0	0.0	26.208780771460077	3.746734252756941E-5
28	2.997387402205553E-4	0.0	0.0	26.762623028702606	3.746734252756941E-5
29	2.997387402205553E-4	0.0	0.0	27.36048941341503	3.746734252756941E-5
30	2.997387402205553E-4	0.0	0.0	27.93119197479497	3.746734252756941E-5
31	2.997387402205553E-4	0.0	0.0	28.50676529070349	3.746734252756941E-5
32	3.3720608274812465E-4	0.0	0.0	29.180915184802046	3.746734252756941E-5
33	3.3720608274812465E-4	0.0	0.0	29.769639537937746	3.746734252756941E-5
34	3.3720608274812465E-4	0.0	0.0	30.40062705344454	3.746734252756941E-5
35	3.3720608274812465E-4	0.0	0.0	30.95109724985959	3.746734252756941E-5
36	3.3720608274812465E-4	0.0	0.0	31.502728933892996	3.746734252756941E-5
37	3.3720608274812465E-4	0.0	0.0	32.26953556606223	3.746734252756941E-5
38	3.3720608274812465E-4	0.0	0.0	32.94226170114474	3.746734252756941E-5
39	3.7467342527569406E-4	0.0	0.0	33.83462139812386	3.746734252756941E-5
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTCTAG	25	3.892197E-5	45.0	37
ATACTAC	20	7.034965E-4	45.0	12
CGTACGT	25	3.892197E-5	45.0	19
CGACATA	30	2.1665855E-6	44.999996	14
CGTTTTT	22170	0.0	44.228687	1
CGGTCTA	490	0.0	43.163265	30
TATACGG	165	0.0	42.272728	1
TAAACGG	235	0.0	41.17021	1
CATGCGG	395	0.0	41.012657	1
ACCCGCA	5905	0.0	40.694324	31
CGAATAT	155	0.0	40.64516	14
CGCACTT	5835	0.0	40.565556	34
CCGCACT	5840	0.0	40.53082	33
GCACTTA	5885	0.0	40.297367	35
CACTTAC	5895	0.0	40.229008	36
CCCGCAC	6000	0.0	40.199997	32
TCACGAC	555	0.0	40.135136	24
TACGGGT	180	0.0	40.0	3
CTAGCGG	790	0.0	39.873417	1
ATTGCGG	520	0.0	39.80769	1
>>END_MODULE