FastQCFastQC Report
Thu 26 May 2016
SRR1043350_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1043350_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1148977
Sequences flagged as poor quality0
Sequence length52
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT704186.128756276235295No Hit
CCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTGCT94160.8195116177260294Illumina Single End Adapter 2 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTGCTT85340.7427476790222954Illumina Single End Adapter 2 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTGCT58520.5093226409231865Illumina Single End Adapter 2 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTGC37320.3248106794130779No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCG34020.2960894778572591No Hit
TCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTGCT30030.261362934157951Illumina Single End Adapter 2 (95% over 21bp)
ACCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTGC21300.18538230095119398No Hit
GCCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTGC21300.18538230095119398No Hit
CGTTCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCT20650.17972509458413877No Hit
AGCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTGC18330.15953321955095706No Hit
TCCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTGC16740.14569482243769893No Hit
ACTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTGCT14820.12898430516885892Illumina Single End Adapter 2 (95% over 21bp)
ATCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTGC13240.11523294199970929No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCT13120.11418853467040681No Hit
TGCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTGC13110.11410150072629825No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCC12560.10931463380032845No Hit
CGTCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTG12290.10696471730939783No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCCGG351.01956175E-746.0000041
GATCGCG351.01956175E-746.0000048
GACCGAC351.01956175E-746.0000048
ACGTAGG750.046.0000041
CCGTTGG351.01956175E-746.0000041
TCACGGA253.4171557E-546.02
ACACGTA206.311454E-446.014
TCGCAAA206.311454E-446.019
CGAACGA206.311454E-446.032
GATCGTC206.311454E-446.08
CGACGGT650.046.027
GCTATTG206.311454E-446.06
CGTTGGC253.4171557E-546.02
TGTGGCG206.311454E-446.012
ATAGCGG1600.046.01
TACGGAC206.311454E-446.015
GCCGATC253.4171557E-546.08
TCTACGG1300.046.01
CTACGGC206.311454E-446.02
AGTCTTG301.8614028E-646.017
Produced by FastQC (version 0.11.5)