SRR1043349_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1043349_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1125303
Sequences flagged as poor quality	0
Sequence length	51
%GC	43

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	65595	5.829096696623043	No Hit
CCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTGC	9118	0.8102706559922084	No Hit
CTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTGCT	8293	0.7369570684517859	Illumina Single End Adapter 2 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTGC	5650	0.5020869934586507	No Hit
CGCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTG	3791	0.33688704286756543	No Hit
TCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTGC	3048	0.27086038160388803	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCC	3044	0.2705049217855102	No Hit
GCCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTG	2151	0.19114851733266505	No Hit
ACCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTG	2093	0.18599434996618688	No Hit
CGTTCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTC	2041	0.1813733723272754	No Hit
AGCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTG	1720	0.1528477219024565	No Hit
TCCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTG	1644	0.1460939853532782	No Hit
ACTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTGC	1531	0.13605224548410516	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTC	1335	0.11863471438359269	No Hit
ATCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTG	1318	0.117124010155487	No Hit
TGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTGCTT	1310	0.1164130905187314	Illumina Single End Adapter 2 (95% over 22bp)
TGCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTG	1246	0.11072573342468651	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGC	1158	0.10290561742037478	No Hit
CGTCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCT	1132	0.10059512860091904	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCGGGT	20	7.033223E-4	45.000004	3
TATGCGC	20	7.033223E-4	45.000004	1
TATGCGA	20	7.033223E-4	45.000004	30
CCTCGGT	20	7.033223E-4	45.000004	13
GTCACGG	20	7.033223E-4	45.000004	1
GGCGGTT	20	7.033223E-4	45.000004	3
AGACACG	20	7.033223E-4	45.000004	23
CCAACGG	80	0.0	45.000004	1
AACACGT	20	7.033223E-4	45.000004	40
GAGGCCG	20	7.033223E-4	45.000004	8
TCACGGT	25	3.8907536E-5	45.0	41
CGAACGA	25	3.8907536E-5	45.0	32
GTATTCG	25	3.8907536E-5	45.0	24
CACGGAA	50	2.1827873E-11	45.0	25
GCAACTA	25	3.8907536E-5	45.0	8
TTGCTAC	25	3.8907536E-5	45.0	23
TTGTCGG	35	1.2119017E-7	45.0	1
TCGGGTA	50	2.1827873E-11	45.0	4
ATAGCGG	95	0.0	45.0	1
TAAACGG	75	0.0	45.0	1

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content