##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043349_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1125303
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.169431699728875	33.0	31.0	34.0	30.0	34.0
2	32.477774430531156	34.0	31.0	34.0	31.0	34.0
3	32.547111311353476	34.0	31.0	34.0	31.0	34.0
4	36.117897135260456	37.0	35.0	37.0	35.0	37.0
5	36.06032775172554	37.0	35.0	37.0	35.0	37.0
6	36.29485391934439	37.0	37.0	37.0	35.0	37.0
7	36.222190823271596	37.0	37.0	37.0	35.0	37.0
8	36.12270917255175	37.0	37.0	37.0	35.0	37.0
9	37.69533894426657	39.0	38.0	39.0	35.0	39.0
10	37.547562745322814	39.0	37.0	39.0	35.0	39.0
11	37.30929003121826	39.0	37.0	39.0	34.0	39.0
12	37.33811426789052	39.0	37.0	39.0	34.0	39.0
13	37.3426419373271	39.0	37.0	39.0	34.0	39.0
14	38.75587286268676	40.0	38.0	41.0	35.0	41.0
15	38.86950536877623	41.0	38.0	41.0	35.0	41.0
16	38.91717430771979	41.0	38.0	41.0	35.0	41.0
17	38.87168522611243	41.0	38.0	41.0	35.0	41.0
18	38.623931510002194	40.0	38.0	41.0	35.0	41.0
19	38.35402376071156	40.0	37.0	41.0	35.0	41.0
20	38.09101459784609	40.0	35.0	41.0	34.0	41.0
21	38.045722796437936	40.0	35.0	41.0	34.0	41.0
22	38.01725401958406	40.0	35.0	41.0	34.0	41.0
23	37.972429647837075	40.0	35.0	41.0	34.0	41.0
24	37.88511272075166	40.0	35.0	41.0	34.0	41.0
25	37.870477551379494	40.0	35.0	41.0	34.0	41.0
26	37.8163978946115	40.0	35.0	41.0	34.0	41.0
27	37.80428115805254	40.0	35.0	41.0	34.0	41.0
28	37.743536629689956	40.0	35.0	41.0	34.0	41.0
29	37.640295991390765	40.0	35.0	41.0	34.0	41.0
30	37.586187009187746	40.0	35.0	41.0	34.0	41.0
31	37.258437060951586	39.0	35.0	41.0	33.0	41.0
32	37.0815238206954	39.0	35.0	41.0	33.0	41.0
33	36.74583645471486	39.0	35.0	41.0	32.0	41.0
34	36.34006574229341	39.0	35.0	41.0	31.0	41.0
35	36.20952312399416	39.0	35.0	41.0	30.0	41.0
36	36.13942289321187	39.0	35.0	41.0	30.0	41.0
37	36.00821201045407	39.0	35.0	41.0	30.0	41.0
38	35.989980476369475	39.0	35.0	41.0	30.0	41.0
39	35.93756970344876	39.0	35.0	41.0	30.0	41.0
40	35.867094462558086	39.0	35.0	40.0	29.0	41.0
41	35.787430585362344	39.0	35.0	40.0	29.0	41.0
42	35.754585209494685	39.0	35.0	40.0	29.0	41.0
43	35.64663472860199	39.0	35.0	40.0	28.0	41.0
44	35.58004644082527	38.0	35.0	40.0	27.0	41.0
45	35.566994844943984	38.0	35.0	40.0	27.0	41.0
46	35.494877379692404	38.0	35.0	40.0	27.0	41.0
47	35.34672972523845	38.0	35.0	40.0	26.0	41.0
48	35.314721457243074	38.0	35.0	40.0	26.0	41.0
49	35.26099726029345	38.0	35.0	40.0	26.0	41.0
50	35.16435928812062	38.0	35.0	40.0	26.0	41.0
51	34.71555838738544	38.0	34.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	2.0
14	5.0
15	12.0
16	23.0
17	79.0
18	160.0
19	341.0
20	655.0
21	1099.0
22	1743.0
23	2874.0
24	5443.0
25	10560.0
26	16491.0
27	19345.0
28	18406.0
29	17400.0
30	18722.0
31	21024.0
32	25735.0
33	33325.0
34	59483.0
35	100593.0
36	104839.0
37	87973.0
38	159364.0
39	419570.0
40	37.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.93417061893552	19.976752927878092	25.55676115677289	28.5323152964135
2	33.581355421606446	19.889754137330122	26.629183428818727	19.8997070122447
3	20.464710393556224	21.345717553405617	38.90178911813085	19.28778293490731
4	22.82087579967351	24.210368229712355	36.03393930345871	16.934816667155424
5	20.091210989395744	29.063105670206156	31.760068177193162	19.085615163204935
6	71.63270692426839	1.6488892325000466	22.504960886090235	4.2134429571413206
7	72.2715570828479	1.6183196881195554	18.387936404683895	7.7221868243486425
8	59.059826553381626	3.6429299486449427	27.363741143496462	9.933502354476973
9	32.79205689489853	17.374609327443363	30.578430875950747	19.254902901707364
10	25.576133716874477	19.31071009319268	43.16321915075317	11.94993703917967
11	22.78470776315357	15.629390484162933	41.613858667398915	19.972043085284586
12	18.171727970155594	19.11831746649569	43.882669823149854	18.827284740198863
13	12.773270843497261	22.281376660330597	43.00406201707451	21.94129047909763
14	11.967976624962343	22.225658333799874	44.22062324547255	21.58574179576523
15	12.451135383092376	17.93765767975381	48.05834517458854	21.552861762565282
16	12.962553196783444	25.00322135460405	40.06165450549763	21.972570943114878
17	14.79628153483995	22.12879553329192	41.424398584203544	21.650524347664586
18	16.342442879828813	28.77011791490825	39.484654355315854	15.402784849947082
19	15.86594899329336	23.27133225451278	37.020429164411716	23.842289587782133
20	13.032401051094682	30.17151824886275	35.331372972434984	21.46470772760759
21	13.569678566572737	24.37219131202885	37.04300086287871	25.015129258519703
22	12.257320917121877	26.943143313400924	32.70034826175706	28.099187507720142
23	11.776206052947517	23.45768206429735	43.11709823931865	21.64901364343648
24	16.97791617013373	22.070233528214178	38.4124986781338	22.53935162351829
25	13.197334406821984	25.401336351187194	38.73498959835707	22.66633964363376
26	15.346533333688795	22.601557091734403	36.9118361898973	25.140073384679507
27	21.333365324716986	23.050147382527193	38.16012220708556	17.45636508567026
28	15.92717694700894	22.356378682008312	36.45782513687424	25.258619234108505
29	17.387761340723344	26.392091729960732	34.91406314565943	21.30608378365649
30	25.48975698100867	21.076545605939025	32.22038864199243	21.213308771059882
31	20.14381904251566	23.050236247481788	36.32594954425608	20.47999516574647
32	20.726862009609857	23.898185644222046	35.896909543474074	19.478042802694027
33	25.34917262284025	20.688294619315865	35.74806074452837	18.214472013315525
34	20.025539787950443	18.15448816896427	44.45513786064731	17.364834182437974
35	26.605278756032817	18.9308124123014	38.83469607741204	15.629212754253743
36	27.05982299878344	19.17661287670965	38.23832336712867	15.525240757378237
37	24.568582861682586	18.51981199730206	42.45540978740837	14.456195353606985
38	25.145227552045984	19.112896704265427	36.01989864063279	19.7219771030558
39	30.72221437248457	18.317288765781306	35.57646251720647	15.384034344527652
40	22.74640696772336	17.283611613938646	39.82074161359207	20.14923980474592
41	23.434399446193602	21.621465507512198	32.74584711850942	22.198287927784783
42	23.477498949171913	16.663867420596944	38.8128352985818	21.045798331649344
43	25.73049214300504	19.72722013537687	36.401484755661365	18.140802965956723
44	17.834929792242622	22.25356192954253	38.29741856193399	21.61408971628086
45	21.644126070933783	20.054509763148236	36.63262250256153	21.668741663356446
46	26.247863908653933	17.35274854861313	35.895132244382175	20.504255298350753
47	20.393885024744446	15.860439366108507	39.849711588789866	23.895964020357184
48	21.073968522255786	14.877059778566307	34.27805666562695	29.770915033550967
49	22.226635848300415	14.607354641372147	40.525174108662284	22.640835401665154
50	21.073968522255786	14.32014310812288	40.714989651676035	23.8908987179453
51	18.423304656612487	14.227012635707895	38.73570051799382	28.6139821896858
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	313.0
1	1861.0
2	3409.0
3	39088.5
4	74768.0
5	42224.0
6	9680.0
7	9078.0
8	8476.0
9	8656.5
10	8837.0
11	8822.0
12	8807.0
13	8446.5
14	8086.0
15	7435.5
16	6785.0
17	6036.0
18	5287.0
19	4817.5
20	4348.0
21	4035.5
22	3723.0
23	3418.0
24	3113.0
25	3496.0
26	4538.5
27	5198.0
28	5278.0
29	5358.0
30	6319.0
31	7280.0
32	8569.0
33	9858.0
34	12648.0
35	15438.0
36	17371.5
37	19305.0
38	22491.0
39	25677.0
40	29542.5
41	33408.0
42	41648.0
43	49888.0
44	62001.0
45	74114.0
46	101304.5
47	128495.0
48	159683.5
49	190872.0
50	185158.0
51	179444.0
52	139832.5
53	100221.0
54	76130.5
55	52040.0
56	39958.5
57	27877.0
58	23760.5
59	19644.0
60	15801.5
61	11959.0
62	9686.5
63	7414.0
64	6144.0
65	4874.0
66	3742.5
67	2611.0
68	2143.0
69	1675.0
70	1429.5
71	1184.0
72	998.0
73	812.0
74	683.5
75	402.5
76	250.0
77	208.5
78	167.0
79	139.0
80	111.0
81	67.5
82	24.0
83	26.0
84	28.0
85	17.5
86	7.0
87	3.5
88	0.0
89	0.5
90	1.0
91	1.5
92	2.0
93	1.0
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1125303.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.36926226643827
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.09955782656881	21.896103809293002
2	12.363559030142238	7.509443334659616
3	4.919055851118985	4.481642917376705
4	2.580797236254445	3.1350763249724114
5	1.4614952252638909	2.2192265898593186
6	0.9663521964129981	1.7608441976769003
7	0.6778917340090539	1.4410950300860097
8	0.49324883943466147	1.1983682693926028
9	0.3983624929981945	1.088817752427691
>10	3.44787070044206	22.70007594189281
>50	0.3645758501835755	7.45934173598032
>100	0.21043368905356374	12.154532987970677
>500	0.010315376914252584	2.1674730539525093
>1k	0.005305050984472757	2.852729648809646
>5k	8.841751640787929E-4	2.0640938262800965
>10k+	2.94725054692931E-4	5.871134579369626
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	65595	5.829096696623043	No Hit
CCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTGC	9118	0.8102706559922084	No Hit
CTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTGCT	8293	0.7369570684517859	Illumina Single End Adapter 2 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTGC	5650	0.5020869934586507	No Hit
CGCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTG	3791	0.33688704286756543	No Hit
TCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTGC	3048	0.27086038160388803	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCC	3044	0.2705049217855102	No Hit
GCCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTG	2151	0.19114851733266505	No Hit
ACCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTG	2093	0.18599434996618688	No Hit
CGTTCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTC	2041	0.1813733723272754	No Hit
AGCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTG	1720	0.1528477219024565	No Hit
TCCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTG	1644	0.1460939853532782	No Hit
ACTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTGC	1531	0.13605224548410516	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTC	1335	0.11863471438359269	No Hit
ATCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTG	1318	0.117124010155487	No Hit
TGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTGCTT	1310	0.1164130905187314	Illumina Single End Adapter 2 (95% over 22bp)
TGCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTG	1246	0.11072573342468651	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGC	1158	0.10290561742037478	No Hit
CGTCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCT	1132	0.10059512860091904	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.7584623874636431	0.0
2	0.0	0.0	0.0	2.531229366668355	0.0
3	0.0	0.0	0.0	4.190160339037575	0.0
4	0.0	0.0	0.0	5.203931741051077	0.0
5	0.0	0.0	0.0	10.597412430252119	0.0
6	0.0	0.0	0.0	11.927898530440245	0.0
7	0.0	0.0	0.0	16.4306857797411	0.0
8	0.0	0.0	0.0	21.982968142802427	0.0
9	0.0	0.0	0.0	26.349703146619177	0.0
10	0.0	0.0	0.0	28.863248387323235	0.0
11	0.0	0.0	0.0	31.082739493274257	0.0
12	0.0	0.0	0.0	32.61654860957449	0.0
13	0.0	0.0	0.0	33.2617081799302	0.0
14	0.0	0.0	0.0	33.7358915776462	0.0
15	0.0	0.0	0.0	35.23539882147297	0.0
16	0.0	0.0	0.0	36.966843596791264	0.0
17	0.0	0.0	0.0	38.836651106413115	0.0
18	0.0	0.0	0.0	39.85602100056607	0.0
19	0.0	0.0	0.0	40.95759097771889	0.0
20	0.0	0.0	0.0	42.141538767780766	0.0
21	0.0	0.0	0.0	43.325842017661024	0.0
22	4.4432477297225723E-4	0.0	0.0	44.42385739663006	0.0
23	4.4432477297225723E-4	0.0	0.0	45.29837741479406	0.0
24	6.220546821611602E-4	0.0	0.0	46.06430445844364	0.0
25	6.220546821611602E-4	0.0	0.0	46.757006779507385	0.0
26	6.220546821611602E-4	0.0	0.0	47.52195630865642	0.0
27	6.220546821611602E-4	0.0	0.0	48.19288671584453	0.0
28	6.220546821611602E-4	0.0	0.0	48.88932136500125	0.0
29	6.220546821611602E-4	0.0	0.0	49.66431263401946	0.0
30	6.220546821611602E-4	0.0	0.0	50.33702034029946	0.0
31	6.220546821611602E-4	0.0	0.0	50.933926240310385	0.0
32	6.220546821611602E-4	0.0	0.0	51.52621116268241	0.0
33	6.220546821611602E-4	0.0	0.0	52.13058171887927	0.0
34	6.220546821611602E-4	0.0	0.0	52.80417807470521	0.0
35	6.220546821611602E-4	0.0	0.0	53.34367721404813	0.0
36	6.220546821611602E-4	0.0	0.0	53.86646974192728	0.0
37	6.220546821611602E-4	0.0	0.0	54.40819050513506	0.0
38	6.220546821611602E-4	0.0	0.0	54.925295675920175	0.0
39	6.220546821611602E-4	0.0	0.0	55.47039330740254	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCGGGT	20	7.033223E-4	45.000004	3
TATGCGC	20	7.033223E-4	45.000004	1
TATGCGA	20	7.033223E-4	45.000004	30
CCTCGGT	20	7.033223E-4	45.000004	13
GTCACGG	20	7.033223E-4	45.000004	1
GGCGGTT	20	7.033223E-4	45.000004	3
AGACACG	20	7.033223E-4	45.000004	23
CCAACGG	80	0.0	45.000004	1
AACACGT	20	7.033223E-4	45.000004	40
GAGGCCG	20	7.033223E-4	45.000004	8
TCACGGT	25	3.8907536E-5	45.0	41
CGAACGA	25	3.8907536E-5	45.0	32
GTATTCG	25	3.8907536E-5	45.0	24
CACGGAA	50	2.1827873E-11	45.0	25
GCAACTA	25	3.8907536E-5	45.0	8
TTGCTAC	25	3.8907536E-5	45.0	23
TTGTCGG	35	1.2119017E-7	45.0	1
TCGGGTA	50	2.1827873E-11	45.0	4
ATAGCGG	95	0.0	45.0	1
TAAACGG	75	0.0	45.0	1
>>END_MODULE