FastQCFastQC Report
Thu 26 May 2016
SRR1043348_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1043348_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences756131
Sequences flagged as poor quality0
Sequence length52
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT457576.051464627161166No Hit
CTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGCTT66270.8764354324845828Illumina Single End Adapter 1 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGCT65000.859639401109067No Hit
GCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGCT51620.6826859366961545No Hit
TCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGCT27090.3582712519391481No Hit
CGCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGC24310.3215051360147911No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCG23190.30669288787260407No Hit
ACCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGC18240.24122804117275976No Hit
GCCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGC16180.21398408476838007No Hit
AGCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGC15310.20247814201507414No Hit
TCCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGC13870.1834338229751194No Hit
ACTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGCT13520.17880499543068595No Hit
CGTTCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCT12080.15976067639073124No Hit
TGCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGC11140.14732896812853857No Hit
TGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGCTTG10320.1364842864530088Illumina Single End Adapter 1 (95% over 22bp)
CGGCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTG10070.1331779810641278No Hit
GGCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGC9710.1284169013041391No Hit
AGCTACCATTACATTTATTATTTTACTTCTACTCACAATTCTAGAATTTGCA9660.12775564022636288No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCC8960.11849798513749601No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCT8770.11598519304194643No Hit
ATAGCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCT8680.11479492310194928No Hit
ATCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGC8510.11254663543751016No Hit
CGTCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTG8510.11254663543751016No Hit
GTCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGC7610.10064393603753848No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAATGG750.046.0000041
GGTACTC301.8605515E-646.07
CTGTCGG253.416038E-546.01
AGTCCAG206.310078E-446.023
CGTATGG206.310078E-446.01
CGCGGAA206.310078E-446.03
GACACAA253.416038E-546.022
ACCGCAG253.416038E-546.01
AAGACGG253.416038E-546.01
CGTCTAT405.6061253E-946.020
CTCCACG206.310078E-446.018
TGACGTA206.310078E-446.011
ACTTATG253.416038E-546.029
TCGATGG301.8605515E-646.01
TGTGATT501.6370905E-1146.042
AAAGTGT253.416038E-546.037
GCCGATG253.416038E-546.08
GTAGCGG1050.046.01
GCTTATC206.310078E-446.09
CTTGCGG1350.046.01
Produced by FastQC (version 0.11.5)