##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043348_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	756131
Sequences flagged as poor quality	0
Sequence length	52
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.15743700496343	33.0	31.0	34.0	30.0	34.0
2	32.47742785311011	34.0	31.0	34.0	31.0	34.0
3	32.54885198464287	34.0	31.0	34.0	31.0	34.0
4	36.0878207612173	37.0	35.0	37.0	35.0	37.0
5	35.97667996683114	37.0	35.0	37.0	35.0	37.0
6	36.25270488843864	37.0	36.0	37.0	35.0	37.0
7	36.17457953714369	37.0	37.0	37.0	35.0	37.0
8	36.13800121936543	37.0	36.0	37.0	35.0	37.0
9	37.68062280213349	39.0	38.0	39.0	35.0	39.0
10	37.53411908783002	39.0	37.0	39.0	35.0	39.0
11	37.26245055420291	39.0	37.0	39.0	34.0	39.0
12	37.24815408970139	39.0	37.0	39.0	34.0	39.0
13	37.25550995793057	39.0	37.0	39.0	34.0	39.0
14	38.653184434972246	40.0	38.0	41.0	34.0	41.0
15	38.75956018203195	40.0	38.0	41.0	35.0	41.0
16	38.78486003086767	40.0	38.0	41.0	35.0	41.0
17	38.771912538964806	40.0	38.0	41.0	35.0	41.0
18	38.50562402546649	40.0	38.0	41.0	35.0	41.0
19	38.245103031088526	40.0	37.0	41.0	34.0	41.0
20	37.94382719396507	40.0	35.0	41.0	34.0	41.0
21	37.851847100568556	40.0	35.0	41.0	34.0	41.0
22	37.80630737266426	40.0	35.0	41.0	34.0	41.0
23	37.804141081373466	40.0	35.0	41.0	34.0	41.0
24	37.71616690758612	40.0	35.0	41.0	34.0	41.0
25	37.59886845004371	39.0	35.0	41.0	34.0	41.0
26	37.583261366086035	39.0	35.0	41.0	34.0	41.0
27	37.605551154495714	40.0	35.0	41.0	34.0	41.0
28	37.60074907654891	40.0	35.0	41.0	33.0	41.0
29	37.56355975353477	40.0	35.0	41.0	33.0	41.0
30	37.54253297378364	40.0	35.0	41.0	33.0	41.0
31	37.42540908916577	40.0	35.0	41.0	33.0	41.0
32	37.27344997097064	40.0	35.0	41.0	33.0	41.0
33	37.12013262252176	40.0	35.0	41.0	33.0	41.0
34	36.933478458097866	40.0	35.0	41.0	32.0	41.0
35	36.802706144834694	40.0	35.0	41.0	32.0	41.0
36	36.75136318971184	40.0	35.0	41.0	32.0	41.0
37	36.68971514195292	40.0	35.0	41.0	31.0	41.0
38	36.62571300475711	40.0	35.0	41.0	31.0	41.0
39	36.61949318305955	40.0	35.0	41.0	31.0	41.0
40	36.59692301995289	40.0	35.0	41.0	31.0	41.0
41	36.583426681355476	40.0	35.0	41.0	31.0	41.0
42	36.51432225368355	40.0	35.0	41.0	31.0	41.0
43	36.40459920304815	39.0	35.0	41.0	31.0	41.0
44	36.249496449689275	39.0	35.0	41.0	31.0	41.0
45	36.17829450187864	39.0	35.0	41.0	30.0	41.0
46	36.15817893989269	39.0	35.0	41.0	30.0	41.0
47	36.07602518611193	39.0	35.0	41.0	30.0	41.0
48	36.06861773951868	39.0	35.0	41.0	30.0	41.0
49	36.037103359074024	39.0	35.0	41.0	30.0	41.0
50	35.91288943318023	39.0	35.0	41.0	30.0	41.0
51	35.83264143382562	39.0	35.0	41.0	29.0	41.0
52	35.527653277011524	38.0	35.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	1.0
14	4.0
15	8.0
16	23.0
17	36.0
18	126.0
19	237.0
20	432.0
21	774.0
22	1247.0
23	2095.0
24	3793.0
25	6872.0
26	9475.0
27	10311.0
28	9724.0
29	9859.0
30	11213.0
31	13546.0
32	17125.0
33	22497.0
34	38536.0
35	70951.0
36	74864.0
37	58104.0
38	91347.0
39	301204.0
40	1726.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.66971728443881	20.079853887752254	25.41847907307067	27.831949754738268
2	33.130767023174556	19.988996615665805	26.920731989562658	19.959504371596985
3	20.96898553293014	22.052924691620895	37.96392424064084	19.01416553480812
4	22.18451564609836	24.142377445178152	35.98331506048555	17.689791848237938
5	19.788370004668504	28.85174658888473	31.257678894265677	20.10220451218109
6	71.23196906356173	1.4902179648764566	22.627296063777308	4.650516907784498
7	71.67250119357624	1.5087332750541904	18.025844727963804	8.792920803405758
8	57.5771923119142	3.4803493045517246	28.152132368597506	10.790326014936564
9	30.541533147034045	19.942047079143695	29.90606125129111	19.61035852253115
10	24.097014935242704	19.436579111291564	44.58288312474954	11.883522828716188
11	21.670319032019584	15.62850881659395	40.59600783462125	22.105164316765215
12	16.485370921176358	19.84735449280614	45.14455828421266	18.522716301804845
13	11.856675628958474	23.906836249274267	43.13128280681522	21.105205314952038
14	10.052887661000542	22.98292227140535	45.95301607790184	21.01117398969226
15	10.195455549369091	19.063627863425783	49.361816933838185	21.379099653366943
16	10.3319398358221	26.14110517886451	39.41036672216851	24.116588263144877
17	12.34534756543509	22.44822656391551	42.14838434080867	23.05804152984073
18	14.468524633958928	30.000489333197557	39.44938112575731	16.081604907086206
19	14.502645705572181	23.485877447161936	36.90775804721669	25.103718800049197
20	11.490998252948232	31.957954375630678	35.263466251218375	21.287581120202717
21	12.79526960275402	24.443647992212988	36.24874525710492	26.512337147928072
22	10.527276358197192	28.381589962585846	31.723338945235678	29.367794733981277
23	10.325856233906558	23.392507382979932	43.50846612557877	22.77317025753474
24	16.402052025376555	21.79714890673706	38.49954571363956	23.301253354246818
25	11.046498556467068	26.025119985822563	38.088902584340545	24.839478873369828
26	13.966495223711235	21.271975358767197	36.701576843166066	28.059952574355503
27	19.50336648014696	21.50394574485109	39.83873164835194	19.153956126650012
28	12.936250464535906	21.759324773088263	37.161417796651634	28.143006965724194
29	14.75233788854048	21.826112141943657	34.66806677678868	28.753483192727185
30	18.020819143772705	21.704704608063945	36.03343865018099	24.24103759798236
31	16.042458251281854	19.952362751957004	34.62720084218211	29.377978154579033
32	11.813825911118576	19.459855501229285	39.13686914040028	29.589449447251866
33	9.92817382173195	21.525502855986595	41.9382355702914	26.608087751990066
34	10.525028070532752	15.614754586176204	45.62979166308484	28.230425680206206
35	12.63127685546552	20.235514745460772	41.36042564053054	25.772782758543162
36	12.08719124067126	22.15092358334733	41.668573302774256	24.093311873207156
37	11.785523936989755	21.91921770169455	39.835557595178614	26.459700766137082
38	11.247125167464368	20.088450281763347	36.816239514052455	31.84818503671983
39	18.559614669944757	17.928507097315148	39.13885292363361	24.37302530910649
40	11.831415455787424	15.816439214897947	43.63013816388959	28.72200716542504
41	16.22073423785032	19.243094119934245	34.80217052336169	29.734001118853744
42	17.228496120381255	14.181934082850722	43.04069003915988	25.54887975760814
43	21.19804637027182	19.484718917753668	38.705594665474635	20.61164004649988
44	13.124445367271015	22.18954123028946	38.6971305236791	25.98888287876043
45	16.742204723784635	20.697603986610787	35.93874606384343	26.621445225761143
46	21.3691807372003	15.86867884004227	36.38866810116237	26.373472321595067
47	13.947318652455726	15.578517479114067	41.713274551631926	28.760889316798277
48	15.937317739915438	13.786367706124997	36.187909237949505	34.08840531601005
49	18.414401737265106	13.915974877369134	41.6179207042166	26.051702681149163
50	17.11780101596152	13.517631204116748	41.02397600415801	28.340591775763723
51	14.20282993290845	12.929902358189254	39.69669276884561	33.17057494005668
52	12.985712793153567	13.076173308593352	49.25403137816066	24.684082520092417
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	315.0
1	1248.5
2	2182.0
3	25993.5
4	49805.0
5	27286.5
6	4768.0
7	4515.0
8	4262.0
9	4388.5
10	4515.0
11	4748.0
12	4981.0
13	4923.0
14	4623.0
15	4381.0
16	4007.5
17	3634.0
18	3340.0
19	3046.0
20	2820.5
21	2595.0
22	2513.0
23	2431.0
24	2374.0
25	2317.0
26	3185.5
27	4054.0
28	3548.5
29	3043.0
30	3273.0
31	3503.0
32	4386.0
33	5269.0
34	6541.0
35	7813.0
36	9255.0
37	10697.0
38	12579.5
39	18035.0
40	21608.0
41	31036.5
42	40465.0
43	59232.5
44	78000.0
45	102402.5
46	126805.0
47	129331.5
48	131858.0
49	110163.0
50	88468.0
51	68535.0
52	48602.0
53	37357.5
54	26113.0
55	20149.0
56	14185.0
57	12606.0
58	11027.0
59	9684.5
60	8342.0
61	6790.0
62	5238.0
63	4364.5
64	3011.0
65	2531.0
66	2188.0
67	1845.0
68	1514.0
69	1183.0
70	1209.5
71	1236.0
72	1122.0
73	1008.0
74	823.0
75	638.0
76	471.0
77	304.0
78	230.5
79	157.0
80	107.5
81	58.0
82	39.0
83	20.0
84	13.0
85	6.0
86	5.0
87	4.0
88	2.5
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	756131.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.432093016216026
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.02053313854664	22.221676565659358
2	11.71531849337538	7.130433242099908
3	4.745243756608067	4.332230981571453
4	2.4517977163418263	2.98453344642642
5	1.449601826409915	2.205720880889159
6	0.9569907386706764	1.7473938704929983
7	0.6485885447771084	1.3816534846736408
8	0.4885380284204062	1.1893787778278873
9	0.4093291987136202	1.1211069824555457
>10	3.522671994071929	22.532613963309117
>50	0.35813917752537405	7.522244524607167
>100	0.21322988694801276	11.937169841938882
>500	0.013054891037633435	2.6256741341926135
>1k	0.0052219564150533734	2.5866075144525205
>5k	0.0013054891037633434	2.421997994666299
>10k+	4.351630345877812E-4	6.059563794737047
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	45757	6.051464627161166	No Hit
CTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGCTT	6627	0.8764354324845828	Illumina Single End Adapter 1 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGCT	6500	0.859639401109067	No Hit
GCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGCT	5162	0.6826859366961545	No Hit
TCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGCT	2709	0.3582712519391481	No Hit
CGCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGC	2431	0.3215051360147911	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCG	2319	0.30669288787260407	No Hit
ACCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGC	1824	0.24122804117275976	No Hit
GCCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGC	1618	0.21398408476838007	No Hit
AGCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGC	1531	0.20247814201507414	No Hit
TCCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGC	1387	0.1834338229751194	No Hit
ACTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGCT	1352	0.17880499543068595	No Hit
CGTTCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCT	1208	0.15976067639073124	No Hit
TGCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGC	1114	0.14732896812853857	No Hit
TGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGCTTG	1032	0.1364842864530088	Illumina Single End Adapter 1 (95% over 22bp)
CGGCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTG	1007	0.1331779810641278	No Hit
GGCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGC	971	0.1284169013041391	No Hit
AGCTACCATTACATTTATTATTTTACTTCTACTCACAATTCTAGAATTTGCA	966	0.12775564022636288	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCC	896	0.11849798513749601	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCT	877	0.11598519304194643	No Hit
ATAGCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCT	868	0.11479492310194928	No Hit
ATCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGC	851	0.11254663543751016	No Hit
CGTCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTG	851	0.11254663543751016	No Hit
GTCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGC	761	0.10064393603753848	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.8966700214645346	0.0
2	0.0	0.0	0.0	3.0244759175328086	0.0
3	0.0	0.0	0.0	4.943323313023801	0.0
4	0.0	0.0	0.0	6.240453043189606	0.0
5	0.0	0.0	0.0	12.465432577159248	0.0
6	0.0	0.0	0.0	13.852097057255952	0.0
7	0.0	0.0	0.0	19.102642267014577	0.0
8	0.0	0.0	0.0	25.341376031401968	0.0
9	0.0	0.0	0.0	30.28681537987465	0.0
10	0.0	0.0	0.0	32.996795528817096	0.0
11	0.0	0.0	0.0	35.6652484820752	0.0
12	0.0	0.0	0.0	37.55949696547292	0.0
13	0.0	0.0	0.0	38.26731082312457	0.0
14	1.3225221555524108E-4	0.0	0.0	38.79274887552554	0.0
15	1.3225221555524108E-4	0.0	0.0	40.47393903966376	0.0
16	1.3225221555524108E-4	0.0	0.0	42.4003248114414	0.0
17	1.3225221555524108E-4	0.0	0.0	44.84011368400449	0.0
18	1.3225221555524108E-4	0.0	0.0	45.888741501142	0.0
19	1.3225221555524108E-4	0.0	0.0	47.1770103328656	0.0
20	1.3225221555524108E-4	0.0	0.0	48.39849179573381	0.0
21	1.3225221555524108E-4	0.0	0.0	49.49618518484231	0.0
22	3.9675664666572326E-4	0.0	0.0	50.59387857395081	0.0
23	3.9675664666572326E-4	0.0	0.0	51.41278429266886	0.0
24	0.002909548742215304	0.0	0.0	52.101421579065004	0.0
25	0.002909548742215304	0.0	0.0	52.7435060855857	0.0
26	0.002909548742215304	0.0	0.0	53.355437086959796	0.0
27	0.003306305388881027	0.0	0.0	53.96128448641836	0.0
28	0.003306305388881027	0.0	0.0	54.58247314288133	0.0
29	0.003306305388881027	0.0	0.0	55.31276987717737	0.0
30	0.003306305388881027	0.0	0.0	55.947580511842524	0.0
31	0.003306305388881027	0.0	0.0	56.46905099777684	0.0
32	0.003306305388881027	0.0	0.0	56.98205734191562	0.0
33	0.003306305388881027	0.0	0.0	57.48289648222332	0.0
34	0.003306305388881027	0.0	0.0	58.075518660126356	0.0
35	0.003306305388881027	0.0	0.0	58.57053870294962	0.0
36	0.003306305388881027	0.0	0.0	59.05656559511513	0.0
37	0.003306305388881027	0.0	0.0	59.535318615425105	0.0
38	0.003306305388881027	0.0	0.0	60.013410374657305	0.0
39	0.0035708098199915095	0.0	0.0	60.46121637652735	0.0
40	0.0035708098199915095	0.0	0.0	60.95094633072841	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATAATGG	75	0.0	46.000004	1
GGTACTC	30	1.8605515E-6	46.0	7
CTGTCGG	25	3.416038E-5	46.0	1
AGTCCAG	20	6.310078E-4	46.0	23
CGTATGG	20	6.310078E-4	46.0	1
CGCGGAA	20	6.310078E-4	46.0	3
GACACAA	25	3.416038E-5	46.0	22
ACCGCAG	25	3.416038E-5	46.0	1
AAGACGG	25	3.416038E-5	46.0	1
CGTCTAT	40	5.6061253E-9	46.0	20
CTCCACG	20	6.310078E-4	46.0	18
TGACGTA	20	6.310078E-4	46.0	11
ACTTATG	25	3.416038E-5	46.0	29
TCGATGG	30	1.8605515E-6	46.0	1
TGTGATT	50	1.6370905E-11	46.0	42
AAAGTGT	25	3.416038E-5	46.0	37
GCCGATG	25	3.416038E-5	46.0	8
GTAGCGG	105	0.0	46.0	1
GCTTATC	20	6.310078E-4	46.0	9
CTTGCGG	135	0.0	46.0	1
>>END_MODULE