FastQCFastQC Report
Thu 26 May 2016
SRR1043347_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1043347_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences751764
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT431805.743823859615517No Hit
CTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGCT65180.8670274181791094No Hit
CCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGC63790.8485375729617273No Hit
GCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGC53570.7125906534497528No Hit
TCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGC26230.3489126906848426No Hit
CGCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTG24970.3321521115669279No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCC22370.2975667895775802No Hit
ACCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTG19420.2583257511665895No Hit
GCCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTG15960.2123006688269191No Hit
AGCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTG15740.20937421850474353No Hit
ACTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGC13950.1855635545197695No Hit
TCCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTG13010.1730596304159284No Hit
TGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGCTT12500.16627558648724866Illumina Single End Adapter 1 (95% over 21bp)
CGTTCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTC11980.15935852208937912No Hit
TGCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTG11850.15762925598991173No Hit
CGGCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCT10370.1379422265498215No Hit
GGCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTG9880.13142422355952135No Hit
AGCTACCATTACATTTATTATTTTACTTCTACTCACAATTCTAGAATTTGC9090.12091560649352723No Hit
CGTCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCT8670.11532874678755567No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGC8660.11519572631836587No Hit
ATAGCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTC8470.1126683374037597No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTC8400.11173719411943109No Hit
ATCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTG8090.10761355957454734No Hit
GTCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTG7650.10176065893019617No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCATGT207.0317264E-445.0000049
TCGTTTC207.0317264E-445.00000445
GGTACTC351.2111741E-745.0000047
ATAGGTT207.0317264E-445.00000434
CAACGCA406.8102963E-945.00000416
GCGAAAA207.0317264E-445.00000410
CAGTTGA406.8102963E-945.00000443
AACTTCG750.045.00000410
TTGTCGC207.0317264E-445.00000437
GTGATGA351.2111741E-745.00000440
GGTCATG207.0317264E-445.0000048
TGACGTA351.2111741E-745.00000411
TATGCGC207.0317264E-445.0000041
CCATGGT207.0317264E-445.00000435
CCGTCGG207.0317264E-445.0000041
ATCGTAA207.0317264E-445.00000433
GAGACTA207.0317264E-445.00000410
TGCGCTC207.0317264E-445.00000414
CGCTCAG207.0317264E-445.00000416
TGACCAA207.0317264E-445.00000414