FastQCFastQC Report
Thu 26 May 2016
SRR1043346_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1043346_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2016921
Sequences flagged as poor quality0
Sequence length52
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1259166.242981257074521No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC94380.4679409852939208No Hit
CCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTCTGCT87180.4322430080305575Illumina PCR Primer Index 8 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTCTGCTT69840.34627037945462413Illumina PCR Primer Index 8 (96% over 25bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA66960.3319911885492788No Hit
CGCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTCTGC66610.33025587021008757Illumina PCR Primer Index 8 (95% over 23bp)
CGTTCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTCT58840.2917318030800413Illumina PCR Primer Index 8 (95% over 21bp)
CGTTTCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTC57650.28583172072679097No Hit
GCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTCTGCT46190.22901244024927103Illumina PCR Primer Index 8 (95% over 24bp)
CGTTTTCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTT41340.2049658861204777No Hit
CGTCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTCTG32360.16044257558922734Illumina PCR Primer Index 8 (95% over 22bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCG31550.15642655314709897No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCT25360.1257362088054019No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC23790.11795206654102962No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC23410.11606800662990766No Hit
TCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTCTGCT20530.10178881572456235Illumina PCR Primer Index 8 (95% over 24bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGAA206.312592E-446.00000443
ATAACGG405.6152203E-946.0000041
CGTTTTT299900.045.4554861
ACTACGG1000.043.6999971
TCGATGG1800.043.4444431
ATTACGG1750.043.371431
CGGTCTA1600.043.12500430
CGTCATA601.8189894E-1242.16666837
CACGACC4000.041.97526
AATGCGG2250.041.911111
ACACGAC3900.041.87179625
TAGTAGG4600.041.51
CGATACG508.731149E-1041.3999989
CACTTAC24300.041.36213736
ACGGGAT3950.041.3417744
GTTTTTT335150.041.2030452
CGCACTT24550.041.1283134
GCACTTA24450.041.10838335
ACCCGCA24850.041.09456631
CCGCACT24600.040.9512233
Produced by FastQC (version 0.11.5)