FastQCFastQC Report
Thu 26 May 2016
SRR1043345_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1043345_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1958415
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1165295.950168886574092No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC92240.4709931245420404No Hit
CCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTCTGC78530.4009875332858459Illumina PCR Primer Index 8 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTCTGCT68840.351508745592737Illumina PCR Primer Index 8 (95% over 24bp)
CGCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTCTG63810.3258247102886773Illumina PCR Primer Index 8 (95% over 22bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG58230.29733228146230495No Hit
CGTTCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTC56980.29094956891159435No Hit
CGTTTCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTT53730.27435451627974666No Hit
GCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTCTGC44550.22747987530732763Illumina PCR Primer Index 8 (95% over 23bp)
CGTTTTCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCT38560.19689391676432216No Hit
CGTCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTCT30970.1581380861564071Illumina PCR Primer Index 8 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCC29540.15083626299839412No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTC23620.12060773635822845No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC22330.11402077700589507No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC21770.1111613217831767No Hit
TCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTCTGC19850.10135747530528513Illumina PCR Primer Index 8 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGTTT207.034503E-445.0000048
ACTACGG1100.045.01
TCGGGTA600.044.9999964
CGTTTTT279650.044.4609341
TACGCGG2900.041.896551
CTTACGG1750.041.1428571
CACTTAC23250.041.12903236
CCGCACT23150.040.9179333
AGGGCGA8250.040.9090925
AATACGG556.184564E-1140.9090921
ACCCGCA23450.040.874231
CGCACTT23300.040.75107234
GCACTTA23550.040.605135
ACTTACT22850.040.56892837
CCCGCAC23750.040.5473732
GAACGTA501.0822987E-940.59
CATGCGG2500.040.4999961
CTTACTG22800.040.46052638
TATGCGG3950.040.443041
ACATACG1950.040.38461716
Produced by FastQC (version 0.11.5)