##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043345_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1958415
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.26679432091768	33.0	31.0	34.0	31.0	34.0
2	32.58043468825555	34.0	31.0	34.0	31.0	34.0
3	32.65645126288351	34.0	31.0	34.0	31.0	34.0
4	36.18354077149123	37.0	37.0	37.0	35.0	37.0
5	36.164491182920884	37.0	37.0	37.0	35.0	37.0
6	36.31137527030788	37.0	37.0	37.0	35.0	37.0
7	36.25368576118953	37.0	37.0	37.0	35.0	37.0
8	36.167119839257765	37.0	37.0	37.0	35.0	37.0
9	37.782747272666924	39.0	38.0	39.0	35.0	39.0
10	37.61885402225779	39.0	37.0	39.0	35.0	39.0
11	37.381725017424806	39.0	37.0	39.0	35.0	39.0
12	37.40172945979274	39.0	37.0	39.0	35.0	39.0
13	37.38156927923857	39.0	37.0	39.0	35.0	39.0
14	38.75628556766569	40.0	38.0	41.0	35.0	41.0
15	38.834918033205426	41.0	38.0	41.0	35.0	41.0
16	38.80437241340574	41.0	38.0	41.0	35.0	41.0
17	38.75287668854661	41.0	38.0	41.0	34.0	41.0
18	38.50705034428351	40.0	38.0	41.0	35.0	41.0
19	38.23137128749524	40.0	37.0	41.0	35.0	41.0
20	37.9106476410771	40.0	35.0	41.0	34.0	41.0
21	37.8723345154117	40.0	35.0	41.0	34.0	41.0
22	37.8257892224069	40.0	35.0	41.0	34.0	41.0
23	37.795216539905994	40.0	35.0	41.0	34.0	41.0
24	37.70232917946401	39.0	35.0	41.0	34.0	41.0
25	37.678958239188326	39.0	35.0	41.0	34.0	41.0
26	37.64869090565585	39.0	35.0	41.0	34.0	41.0
27	37.628697186245	39.0	35.0	41.0	34.0	41.0
28	37.53187654302076	39.0	35.0	41.0	34.0	41.0
29	37.455494366617906	39.0	35.0	41.0	34.0	41.0
30	37.31242203516619	39.0	35.0	41.0	33.0	41.0
31	37.12186436480521	39.0	35.0	41.0	33.0	41.0
32	36.84963503649635	39.0	35.0	41.0	33.0	41.0
33	36.355814268170946	39.0	35.0	41.0	31.0	41.0
34	36.02602614869678	39.0	35.0	41.0	30.0	41.0
35	35.793593799067104	39.0	35.0	41.0	29.0	41.0
36	35.707450157397695	39.0	35.0	41.0	28.0	41.0
37	35.594517505227444	38.0	35.0	41.0	27.0	41.0
38	35.532570982146275	38.0	35.0	41.0	27.0	41.0
39	35.47861255147658	38.0	35.0	41.0	27.0	41.0
40	35.387825869389275	38.0	35.0	41.0	26.0	41.0
41	35.304104084170106	38.0	35.0	41.0	26.0	41.0
42	35.19048312027839	38.0	35.0	40.0	25.0	41.0
43	35.04859899459512	38.0	35.0	40.0	24.0	41.0
44	34.91590801745289	37.0	35.0	40.0	24.0	41.0
45	34.84618786110196	37.0	35.0	40.0	23.0	41.0
46	34.75450249308752	37.0	35.0	40.0	23.0	41.0
47	34.60480694847619	37.0	35.0	40.0	23.0	41.0
48	34.528445196753495	37.0	35.0	40.0	23.0	41.0
49	34.450573550549805	36.0	34.0	40.0	23.0	41.0
50	34.36763045626182	36.0	34.0	40.0	23.0	41.0
51	33.9637758084982	36.0	34.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	0.0
14	14.0
15	25.0
16	63.0
17	155.0
18	324.0
19	678.0
20	1306.0
21	2142.0
22	3489.0
23	5661.0
24	9847.0
25	18725.0
26	31050.0
27	38307.0
28	38784.0
29	36759.0
30	36939.0
31	40058.0
32	46062.0
33	58640.0
34	120348.0
35	178724.0
36	192698.0
37	171933.0
38	275904.0
39	649510.0
40	269.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.728698973404516	16.97566654667167	23.193756175274395	32.101878304649425
2	37.57334374992022	18.603156123702075	22.787100793243518	21.03639933313419
3	24.17766408039154	19.78308989667665	36.85812251233778	19.181123510594027
4	22.37804551129357	22.34189382740635	34.629075042828	20.65098561847208
5	20.58899671417958	29.65566542331426	31.753739631283462	18.001598231222697
6	73.7686343292918	1.9183880842415932	20.94612224681694	3.366855339649666
7	73.38128027001427	1.7449825496638864	19.24331666168815	5.630420518633691
8	63.759315568967764	3.380386690257172	25.182507282675022	7.677790458100045
9	37.20186987946886	15.751513341145774	29.148571676585398	17.898045102799966
10	25.886290699366583	15.830454729972962	39.760367439996116	18.52288713066434
11	23.377629358435264	12.888892293002248	42.209031282950754	21.52444706561173
12	19.996221434169982	14.151392835532818	44.69956572023805	21.152820010059155
13	15.555283226486724	16.9094395212455	45.43868383361034	22.096593418657434
14	15.302323562676962	17.60301059785592	45.71344684349333	21.381218995973786
15	18.23561400418195	15.186107132553623	44.96998848558656	21.608290377677868
16	17.79260269146223	19.393540184281676	41.62365994949998	21.19019717475612
17	18.697211775849347	19.555201527766076	40.87805700017616	20.869529696208414
18	19.219266600797074	26.25163716576926	37.2399619079715	17.28913432546217
19	19.724777434813355	21.46659415905209	36.33356566407018	22.475062742064374
20	18.636193043864555	26.1666704962942	35.97470403361903	19.222432426222223
21	17.203299607080215	20.837514010054047	37.325388132750206	24.633798250115525
22	18.09790059818782	22.485070835343887	35.19693221303963	24.220096353428666
23	17.817827171462636	20.473086654258672	39.34574643270196	22.363339741576734
24	21.245599119696283	18.93469974443619	36.273057549089444	23.54664358677808
25	16.660054176464133	19.99162588113347	38.979787225894405	24.368532716507993
26	17.173990191047352	19.102079998366026	37.847749327900374	25.876180482686255
27	21.80967772407789	20.68126520681265	36.85424182310695	20.654815246002507
28	22.46408447647715	21.144650137994244	33.10830441964548	23.282960965883127
29	22.408120852832518	21.562743340915997	34.496008251570785	21.533127554680696
30	26.449399131440476	19.852023192224326	31.604741589499675	22.093836086835527
31	27.18565778959005	21.69723985978457	29.937526009553643	21.179576341071733
32	27.868352724014063	21.632136191767323	29.132640426058824	21.366870658159787
33	25.155342458059195	23.62359356928945	31.9516547820559	19.269409190595457
34	27.518273706032687	23.44727751778862	29.965201451173524	19.069247325005172
35	29.321772964361486	25.216922868748455	28.139439291467845	17.321864875422214
36	26.033450519935762	26.206753931112658	27.111618324001807	20.64817722494977
37	24.807816525098104	27.768322852919326	29.723066867849766	17.700793754132807
38	23.207440710983114	23.82559365609434	28.353745248070506	24.613220384852035
39	24.964218513440716	20.769346640012458	32.36622472764966	21.90021011889717
40	19.56000132760421	20.102940388017863	35.20408085109642	25.132977433281507
41	21.921094354363095	23.020146393895065	30.950794392404063	24.10796485933778
42	22.112984224487658	17.861331740208282	33.77169803131614	26.25398600398792
43	24.310220254644697	20.186477329881562	33.81765356168126	21.68564885379248
44	22.006520579141807	21.424059762614156	34.451022893513375	22.11839676473066
45	23.05696187988756	22.164760788699027	32.59794272409066	22.180334607322756
46	23.842801449131056	20.115399442916846	32.79958537899271	23.24221372895939
47	22.137391717281577	20.097578909475263	34.14220172945979	23.622827643783364
48	22.41486099728607	18.887212363058904	31.322932064960696	27.374994574694334
49	21.667879382051304	17.495576780202356	36.21765560414927	24.618888233597065
50	21.136122834026498	17.311703597041486	37.16020353193782	24.391970036994202
51	19.28661698363217	16.478019214517865	36.658215955249524	27.577147846600443
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	434.0
1	2721.5
2	5009.0
3	75772.0
4	146535.0
5	83823.0
6	21111.0
7	19065.5
8	17020.0
9	16709.5
10	16399.0
11	15898.5
12	15398.0
13	14545.0
14	13692.0
15	12770.0
16	11848.0
17	10967.5
18	10087.0
19	9237.5
20	8388.0
21	7870.0
22	7352.0
23	7053.0
24	6754.0
25	6965.5
26	7866.0
27	8555.0
28	9063.5
29	9572.0
30	10695.5
31	11819.0
32	13849.5
33	15880.0
34	18358.0
35	20836.0
36	23486.0
37	26136.0
38	27807.0
39	29478.0
40	33056.0
41	36634.0
42	41897.5
43	47161.0
44	55222.0
45	63283.0
46	73635.0
47	83987.0
48	118587.0
49	153187.0
50	187804.0
51	222421.0
52	228157.5
53	233894.0
54	202785.0
55	171676.0
56	146390.0
57	121104.0
58	105027.0
59	88950.0
60	81736.0
61	74522.0
62	70937.0
63	67352.0
64	65195.0
65	63038.0
66	52025.5
67	41013.0
68	33254.5
69	25496.0
70	21507.0
71	17518.0
72	16063.0
73	14608.0
74	12756.0
75	8645.0
76	6386.0
77	4597.0
78	2808.0
79	2292.5
80	1777.0
81	1200.0
82	623.0
83	473.5
84	324.0
85	248.5
86	173.0
87	109.5
88	46.0
89	35.5
90	25.0
91	21.0
92	17.0
93	10.5
94	4.0
95	4.0
96	4.0
97	2.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1958415.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.003984874081524
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.21555887744793	24.071976086094317
2	10.0820415395139	6.45331009860929
3	3.6530211130606034	3.5073369734127597
4	1.8409678418054127	2.356732278512437
5	1.2275782296350968	1.9643697546496708
6	0.8827104788694115	1.695015168835794
7	0.7219697881225593	1.6174137125032764
8	0.6140117669733482	1.5720658642178487
9	0.5401679185277823	1.5558773304624522
>10	4.83588404749166	29.435802874566626
>50	0.21774405346991496	4.795618041237217
>100	0.15706778772497748	8.763091672654053
>500	0.005477185002249421	1.1798681195289473
>1k	0.004510622943028935	2.588388854749871
>5k	0.0011276557357572338	2.435317915406133
>10k+	1.6109367653674767E-4	6.007815254559262
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	116529	5.950168886574092	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	9224	0.4709931245420404	No Hit
CCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTCTGC	7853	0.4009875332858459	Illumina PCR Primer Index 8 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTCTGCT	6884	0.351508745592737	Illumina PCR Primer Index 8 (95% over 24bp)
CGCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTCTG	6381	0.3258247102886773	Illumina PCR Primer Index 8 (95% over 22bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG	5823	0.29733228146230495	No Hit
CGTTCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTC	5698	0.29094956891159435	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTT	5373	0.27435451627974666	No Hit
GCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTCTGC	4455	0.22747987530732763	Illumina PCR Primer Index 8 (95% over 23bp)
CGTTTTCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCT	3856	0.19689391676432216	No Hit
CGTCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTCT	3097	0.1581380861564071	Illumina PCR Primer Index 8 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCC	2954	0.15083626299839412	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTC	2362	0.12060773635822845	No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	2233	0.11402077700589507	No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	2177	0.1111613217831767	No Hit
TCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTCTGC	1985	0.10135747530528513	Illumina PCR Primer Index 8 (95% over 23bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.36064878996535465	0.0
2	0.0	0.0	0.0	1.164819509654491	0.0
3	0.0	0.0	0.0	2.071522123758243	0.0
4	0.0	0.0	0.0	2.6748671757518196	0.0
5	0.0	0.0	0.0	5.575222820495146	0.0
6	0.0	0.0	0.0	6.871066653390624	0.0
7	0.0	0.0	0.0	10.19211964777639	0.0
8	0.0	0.0	0.0	13.787731405243527	0.0
9	0.0	0.0	0.0	17.335600472831345	0.0
10	0.0	0.0	0.0	19.097382321928702	0.0
11	0.0	0.0	0.0	20.509697893449548	0.0
12	0.0	0.0	0.0	21.527612891036885	0.0
13	0.0	0.0	0.0	22.03394071225966	0.0
14	0.0	0.0	0.0	22.36088877995726	0.0
15	0.0	0.0	0.0	23.192428571063846	0.0
16	0.0	0.0	0.0	24.09509731083555	0.0
17	0.0	0.0	0.0	25.21385916672411	0.0
18	0.0	0.0	0.0	25.83216529693655	0.0
19	0.0	0.0	0.0	26.542178241077607	0.0
20	0.0	0.0	0.0	27.300648738903654	0.0
21	0.0	0.0	0.0	28.084854333734167	0.0
22	0.0	0.0	0.0	28.817079117551692	0.0
23	0.0	0.0	0.0	29.480472729222356	0.0
24	0.0	0.0	0.0	30.00845071141714	0.0
25	0.0	0.0	0.0	30.523561145109692	0.0
26	0.0	0.0	0.0	31.06062810997669	0.0
27	0.0	0.0	0.0	31.599992851361943	0.0
28	0.0	0.0	0.0	32.139357592747196	0.0
29	0.0	0.0	0.0	32.69061971032697	0.0
30	0.0	0.0	0.0	33.212112856570236	0.0
31	0.0	0.0	0.0	33.72130013301573	0.0
32	0.0	0.0	0.0	34.24953342371254	0.0
33	0.0	0.0	0.0	34.75509531942923	0.0
34	0.0	0.0	0.0	35.30314054988345	0.0
35	0.0	0.0	0.0	35.7858778655188	0.0
36	0.0	0.0	0.0	36.29327798245009	0.0
37	0.0	0.0	0.0	36.894121011123794	0.0
38	0.0	0.0	0.0	37.57441604562874	0.0
39	0.0	0.0	0.0	38.24899216968824	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGTTT	20	7.034503E-4	45.000004	8
ACTACGG	110	0.0	45.0	1
TCGGGTA	60	0.0	44.999996	4
CGTTTTT	27965	0.0	44.460934	1
TACGCGG	290	0.0	41.89655	1
CTTACGG	175	0.0	41.142857	1
CACTTAC	2325	0.0	41.129032	36
CCGCACT	2315	0.0	40.91793	33
AGGGCGA	825	0.0	40.909092	5
AATACGG	55	6.184564E-11	40.909092	1
ACCCGCA	2345	0.0	40.8742	31
CGCACTT	2330	0.0	40.751072	34
GCACTTA	2355	0.0	40.6051	35
ACTTACT	2285	0.0	40.568928	37
CCCGCAC	2375	0.0	40.54737	32
GAACGTA	50	1.0822987E-9	40.5	9
CATGCGG	250	0.0	40.499996	1
CTTACTG	2280	0.0	40.460526	38
TATGCGG	395	0.0	40.44304	1
ACATACG	195	0.0	40.384617	16
>>END_MODULE