FastQCFastQC Report
Thu 26 May 2016
SRR1043344_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1043344_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2306704
Sequences flagged as poor quality0
Sequence length52
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1032134.47447960379832No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC121090.5249481511281898No Hit
CCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTGCT107020.46395202852208167Illumina PCR Primer Index 5 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTGCTT96780.41955968342708905Illumina PCR Primer Index 5 (96% over 25bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA80110.34729206694920545No Hit
GCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTGCT73620.31915668416927356Illumina PCR Primer Index 5 (95% over 24bp)
CGCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTGC50520.21901379630849907Illumina PCR Primer Index 5 (95% over 23bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCG41840.18138434753657168No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC34530.1496941089970798No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC32270.1398965797085365No Hit
AGCCCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCT30140.13066262511358198Illumina PCR Primer Index 5 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTGCT29530.1280181592436654Illumina PCR Primer Index 5 (95% over 24bp)
CGTTCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCT28710.12446330348410545Illumina PCR Primer Index 5 (95% over 21bp)
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAA28420.12320609839840742No Hit
GCCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTGC27660.11991135403588843Illumina PCR Primer Index 5 (95% over 23bp)
GGGCCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCT23230.10070646255436327Illumina PCR Primer Index 5 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTAACG253.418234E-546.09
CGTTTTT238850.045.114091
CTTACGG1700.043.2941171
CGGTCTA2100.042.71428330
GCGCGAC17700.042.1016968
TCACGAC2300.042.024
ATAGCGG2050.041.5121961
CACTTAC33350.041.2413836
ACCCGCA33900.041.1828931
CCCGCAC34050.041.0014732
TAGGGTA7350.040.99324
CCGCACT33700.040.9495633
ACTTACT33400.040.8353337
CATGCGG4000.040.8249971
CGCACTT34000.040.7235334
GCACTTA33850.040.56425535
GTTTTTT273600.040.5190052
CTTATGA38450.040.43692825
AACGCAA40650.040.34194617
CGCATCG402.9122748E-740.2520
Produced by FastQC (version 0.11.5)