##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043344_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2306704
Sequences flagged as poor quality	0
Sequence length	52
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.2648293842643	33.0	31.0	34.0	31.0	34.0
2	32.601023798458755	34.0	31.0	34.0	31.0	34.0
3	32.72338973704472	34.0	31.0	34.0	31.0	34.0
4	36.172641136444035	37.0	37.0	37.0	35.0	37.0
5	36.104781541108004	37.0	35.0	37.0	35.0	37.0
6	36.27432041562333	37.0	37.0	37.0	35.0	37.0
7	36.195408253508035	37.0	37.0	37.0	35.0	37.0
8	36.17193753511504	37.0	37.0	37.0	35.0	37.0
9	37.73325489529649	39.0	38.0	39.0	35.0	39.0
10	37.62225495772323	39.0	37.0	39.0	35.0	39.0
11	37.424775783975754	39.0	37.0	39.0	35.0	39.0
12	37.43378387517428	39.0	37.0	39.0	35.0	39.0
13	37.42938582496931	39.0	37.0	39.0	35.0	39.0
14	38.66478013650646	40.0	38.0	41.0	34.0	41.0
15	38.78867119491708	40.0	38.0	41.0	35.0	41.0
16	38.74385486824491	40.0	38.0	41.0	35.0	41.0
17	38.65316096040064	40.0	38.0	41.0	34.0	41.0
18	38.49334288231173	40.0	38.0	41.0	34.0	41.0
19	38.27259544354195	40.0	37.0	41.0	34.0	41.0
20	37.874788442730406	40.0	35.0	41.0	34.0	41.0
21	37.87677222565184	40.0	35.0	41.0	34.0	41.0
22	37.8515561597847	40.0	35.0	41.0	34.0	41.0
23	37.85644625404907	40.0	35.0	41.0	34.0	41.0
24	37.78760994041715	40.0	35.0	41.0	34.0	41.0
25	37.676512027551	40.0	35.0	41.0	34.0	41.0
26	37.587285147986044	40.0	35.0	41.0	33.0	41.0
27	37.622779082188266	40.0	35.0	41.0	33.0	41.0
28	37.58897110335786	40.0	35.0	41.0	33.0	41.0
29	37.57384129042998	40.0	35.0	41.0	33.0	41.0
30	37.53194774882256	40.0	35.0	41.0	33.0	41.0
31	37.411619349513416	40.0	35.0	41.0	33.0	41.0
32	37.181604575186064	40.0	35.0	41.0	33.0	41.0
33	36.79404726397492	39.0	35.0	41.0	31.0	41.0
34	36.64614705657943	39.0	35.0	41.0	31.0	41.0
35	36.54383570670532	39.0	35.0	41.0	31.0	41.0
36	36.481516917645266	39.0	35.0	41.0	31.0	41.0
37	36.402072394204026	39.0	35.0	41.0	31.0	41.0
38	36.26824291283147	39.0	35.0	41.0	31.0	41.0
39	36.234235948782334	39.0	35.0	41.0	31.0	41.0
40	36.14378134342334	39.0	35.0	41.0	31.0	41.0
41	36.09484615277903	39.0	35.0	41.0	31.0	41.0
42	36.01281568853221	38.0	35.0	41.0	30.0	41.0
43	35.90547031608737	38.0	35.0	41.0	30.0	41.0
44	35.71951017555785	38.0	35.0	40.0	30.0	41.0
45	35.590971793520104	38.0	35.0	40.0	29.0	41.0
46	35.54150554210683	38.0	35.0	40.0	29.0	41.0
47	35.454843794435696	37.0	35.0	40.0	29.0	41.0
48	35.35255238643536	37.0	35.0	40.0	29.0	41.0
49	35.279404726397495	37.0	35.0	40.0	29.0	41.0
50	35.167331829311436	37.0	35.0	40.0	28.0	41.0
51	35.038104585590524	36.0	35.0	40.0	28.0	41.0
52	34.652152161699114	36.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	2.0
11	2.0
12	0.0
13	8.0
14	16.0
15	35.0
16	74.0
17	183.0
18	403.0
19	822.0
20	1500.0
21	2539.0
22	4043.0
23	6470.0
24	10960.0
25	19214.0
26	28255.0
27	34659.0
28	35324.0
29	35391.0
30	37208.0
31	42653.0
32	52263.0
33	68543.0
34	131882.0
35	197123.0
36	232338.0
37	238694.0
38	336964.0
39	787017.0
40	2118.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.247572293627616	17.217900519529163	22.93523573028876	28.59929145655446
2	35.501694192232726	19.640057848774703	23.23458059638341	21.62366736260916
3	26.90765698589849	20.639882707100693	32.68750563574694	19.764954671253875
4	23.59440136229009	23.307108324258337	30.781192558733157	22.31729775471842
5	21.772624489314623	31.29564955018069	28.066063092620464	18.865662867884218
6	76.8016182397048	2.350670046958778	17.651202755099916	3.1965089582365143
7	75.97541773890364	2.5767502028868896	15.984755738057418	5.463076320152044
8	65.03586936165195	4.24987341245344	22.50895650243811	8.2053007234565
9	38.151145530592565	15.41589211272881	27.200152251871067	19.23281010480755
10	24.65101720897003	15.82587102636489	36.322085538499955	23.201026226165126
11	21.677770533193684	13.222719516678344	40.09443777788568	25.005072172242297
12	20.96272430272805	14.879412356331805	42.59735969591244	21.56050364502771
13	18.1056173657305	18.337333268594495	42.677517358100566	20.879532007574443
14	15.12664823921925	18.62289223064598	44.23419736559177	22.016262164543
15	18.266756376197378	16.936546691729845	42.441206153888835	22.35549077818394
16	19.326580263440825	19.724767460411044	37.25900679064154	23.689645485506595
17	18.049606711567673	19.5581661106063	39.684675623747125	22.707551554078893
18	21.66684585451796	23.756060595551055	35.5726178998259	19.004475650105086
19	22.088529781020885	19.709464239885136	33.65056808329114	24.55143789580284
20	20.082290575643864	23.950147049643128	36.281031289666984	19.68653108504602
21	20.573424245156726	19.13062967766996	34.381394405177254	25.914551671996062
22	20.620287648523608	21.303123417655666	32.39492366597535	25.68166526784538
23	18.685318965935814	19.640014496875192	36.876989852187364	24.79767668500163
24	24.236659753483757	18.22708938814863	33.40051432693575	24.13573653143186
25	17.99194868522359	20.256001636967724	34.810968377390424	26.941081300418258
26	18.896009197538998	20.309714640456686	34.71446704908822	26.07980911291609
27	22.45754114962301	19.724507349014004	34.3137654419466	23.50418605941638
28	21.892535843350515	21.860065270619895	31.267600871199775	24.979798014829818
29	23.51359342160936	18.884260832772647	32.86910674278104	24.73303900283695
30	23.872850612822454	19.757411440739688	29.45245683884885	26.91728110758901
31	26.68890330098704	21.680371647164094	28.890919684536897	22.739805367311973
32	24.908744251538124	18.993420915730844	31.522683447897954	24.575151384833077
33	22.397585472605066	20.812640026635407	33.83290617261686	22.956868328142665
34	20.26688296374394	24.639528955600717	32.40567493705304	22.6879131436023
35	25.14089367339719	22.07821202893826	33.12661702585161	19.65427727181294
36	24.382538895324238	22.428105209857875	32.285590175419124	20.903765719398763
37	21.401879044732226	26.173709327247884	33.30843489238325	19.11597673563665
38	19.377778856758386	21.73304420506489	34.887397776220965	24.00177916195576
39	23.028008795233372	18.705824414402542	39.28856931795324	18.97759747241085
40	19.2153392893063	18.152654176695407	39.40050392247987	23.231502611518426
41	22.6485496188501	22.69775402479035	31.350836518252883	23.302859838106667
42	20.171422081029903	17.561984545914864	34.80802044822396	27.458572924831277
43	23.463348570080946	19.613309726778986	35.324384923249795	21.59895677989027
44	23.066938800990506	20.245076958292003	33.74576885460813	22.942215386109357
45	22.83704367790579	21.503452545276723	32.337959270023376	23.32154450679411
46	22.91785161858652	21.010541447884084	32.91761751832919	23.153989415200215
47	21.160452316378695	20.202158577780242	35.51248881520993	23.124900290631135
48	22.296055323960072	18.71527512849503	32.232007227628685	26.75666231991621
49	21.04609000547968	18.863798736205425	36.37458468880273	23.715526569512168
50	21.629606572841595	16.943136180454882	36.87083388245739	24.55642336424613
51	20.613871567396597	16.57295431056607	35.690361658886445	27.122812463150886
52	17.515511309643543	17.07457913975959	40.22739805367312	25.18251149692375
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	524.0
1	3787.5
2	7051.0
3	67094.0
4	127137.0
5	71468.0
6	15799.0
7	14780.5
8	13762.0
9	13559.5
10	13357.0
11	13115.0
12	12873.0
13	12527.0
14	11429.5
15	10678.0
16	10580.5
17	10483.0
18	9657.5
19	8832.0
20	8713.0
21	8594.0
22	8199.0
23	7804.0
24	7953.0
25	8102.0
26	8817.5
27	9533.0
28	9820.0
29	10107.0
30	11862.5
31	13618.0
32	15544.0
33	17470.0
34	18800.5
35	20131.0
36	22789.5
37	25448.0
38	28378.0
39	36720.0
40	42132.0
41	55155.5
42	68179.0
43	94587.5
44	120996.0
45	162057.0
46	203118.0
47	227193.5
48	251269.0
49	237063.5
50	222858.0
51	196121.5
52	169385.0
53	146793.0
54	124201.0
55	117972.5
56	111744.0
57	107314.0
58	102884.0
59	98194.5
60	93505.0
61	90383.5
62	87262.0
63	90012.0
64	82646.5
65	72531.0
66	63469.5
67	54408.0
68	43422.5
69	32437.0
70	27998.0
71	23559.0
72	22400.5
73	21242.0
74	17259.0
75	13276.0
76	10237.5
77	7199.0
78	5385.5
79	3572.0
80	2717.0
81	1862.0
82	1390.5
83	919.0
84	686.5
85	454.0
86	281.0
87	108.0
88	68.5
89	19.5
90	10.0
91	8.0
92	6.0
93	4.5
94	3.0
95	2.5
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	2306704.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.147868346663024
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.59615628253849	20.43427049514667
2	11.405388606841274	6.420747538957972
3	4.357456897737812	3.679593692513479
4	2.2097407491056966	2.487979667443347
5	1.3200536770091222	1.85783485554906
6	0.8483488377825622	1.4327526838768887
7	0.6417493985003525	1.2644714306376674
8	0.5030408811337119	1.1327622796112868
9	0.4134644133243674	1.04743276850561
>10	5.085798496668627	32.36970458895276
>50	0.4407376592284228	8.211534067492115
>100	0.16428390481138158	8.440633889364946
>500	0.006657847176239716	1.242402719634176
>1k	0.006038512555194161	3.173494104356199
>5k	6.19334621045555E-4	1.3119601876433016
>10k+	4.645009657841662E-4	5.4924250303145685
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	103213	4.47447960379832	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	12109	0.5249481511281898	No Hit
CCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTGCT	10702	0.46395202852208167	Illumina PCR Primer Index 5 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTGCTT	9678	0.41955968342708905	Illumina PCR Primer Index 5 (96% over 25bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA	8011	0.34729206694920545	No Hit
GCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTGCT	7362	0.31915668416927356	Illumina PCR Primer Index 5 (95% over 24bp)
CGCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTGC	5052	0.21901379630849907	Illumina PCR Primer Index 5 (95% over 23bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCG	4184	0.18138434753657168	No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	3453	0.1496941089970798	No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	3227	0.1398965797085365	No Hit
AGCCCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCT	3014	0.13066262511358198	Illumina PCR Primer Index 5 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTGCT	2953	0.1280181592436654	Illumina PCR Primer Index 5 (95% over 24bp)
CGTTCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCT	2871	0.12446330348410545	Illumina PCR Primer Index 5 (95% over 21bp)
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAA	2842	0.12320609839840742	No Hit
GCCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTGC	2766	0.11991135403588843	Illumina PCR Primer Index 5 (95% over 23bp)
GGGCCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCT	2323	0.10070646255436327	Illumina PCR Primer Index 5 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.4335189950682879	0.0
2	0.0	0.0	0.0	1.4435749016778918	0.0
3	0.0	0.0	0.0	2.3190231603187925	0.0
4	0.0	0.0	0.0	2.9267300876055184	0.0
5	0.0	0.0	0.0	5.98165174205273	0.0
6	0.0	0.0	0.0	6.851290846159715	0.0
7	0.0	0.0	0.0	9.657979524030825	0.0
8	0.0	0.0	0.0	12.686716631175912	0.0
9	0.0	0.0	0.0	15.324029437673842	0.0
10	0.0	0.0	0.0	16.92159028639999	0.0
11	0.0	0.0	0.0	18.38515041375053	0.0
12	0.0	0.0	0.0	19.42871733867891	0.0
13	0.0	0.0	0.0	19.91703313472383	0.0
14	0.0	0.0	0.0	20.269440725814842	0.0
15	0.0	0.0	0.0	21.32670685098738	0.0
16	0.0	0.0	0.0	22.409637300667967	0.0
17	0.0	0.0	0.0	23.82420978157579	0.0
18	4.335189950682879E-5	0.0	0.0	24.542767515901478	0.0
19	4.335189950682879E-5	0.0	0.0	25.387349222093516	0.0
20	4.335189950682879E-5	0.0	0.0	26.353012783608126	0.0
21	4.335189950682879E-5	0.0	0.0	27.28898029396056	0.0
22	8.670379901365758E-5	0.0	0.0	28.095195569089054	0.0
23	8.670379901365758E-5	0.0	0.0	28.855414478840803	0.0
24	2.6011139704097274E-4	0.0	0.0	29.44946555778288	0.0
25	2.6011139704097274E-4	0.0	0.0	30.0590366167484	0.0
26	2.6011139704097274E-4	0.0	0.0	30.60284284416206	0.0
27	3.034632965478015E-4	0.0	0.0	31.161735532604098	0.0
28	3.468151960546303E-4	0.0	0.0	31.737318702356262	0.0
29	3.468151960546303E-4	0.0	0.0	32.31758387725517	0.0
30	3.468151960546303E-4	0.0	0.0	32.889568839348264	0.0
31	3.468151960546303E-4	0.0	0.0	33.4490684543834	0.0
32	3.468151960546303E-4	0.0	0.0	34.05257024741796	0.0
33	3.901670955614591E-4	0.0	0.0	34.58137671760226	0.0
34	3.901670955614591E-4	0.0	0.0	35.18782643980329	0.0
35	3.901670955614591E-4	0.0	0.0	35.70189326415526	0.0
36	3.901670955614591E-4	0.0	0.0	36.298675512766266	0.0
37	3.901670955614591E-4	0.0	0.0	36.897712060151626	0.0
38	3.901670955614591E-4	0.0	0.0	37.64171735948782	0.0
39	4.335189950682879E-4	0.0	0.0	38.30677884982208	0.0
40	4.335189950682879E-4	0.0	0.0	38.9404535649134	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACTAACG	25	3.418234E-5	46.0	9
CGTTTTT	23885	0.0	45.11409	1
CTTACGG	170	0.0	43.294117	1
CGGTCTA	210	0.0	42.714283	30
GCGCGAC	1770	0.0	42.101696	8
TCACGAC	230	0.0	42.0	24
ATAGCGG	205	0.0	41.512196	1
CACTTAC	3335	0.0	41.24138	36
ACCCGCA	3390	0.0	41.18289	31
CCCGCAC	3405	0.0	41.00147	32
TAGGGTA	735	0.0	40.9932	4
CCGCACT	3370	0.0	40.94956	33
ACTTACT	3340	0.0	40.83533	37
CATGCGG	400	0.0	40.824997	1
CGCACTT	3400	0.0	40.72353	34
GCACTTA	3385	0.0	40.564255	35
GTTTTTT	27360	0.0	40.519005	2
CTTATGA	3845	0.0	40.436928	25
AACGCAA	4065	0.0	40.341946	17
CGCATCG	40	2.9122748E-7	40.25	20
>>END_MODULE