FastQCFastQC Report
Thu 26 May 2016
SRR1043343_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1043343_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2266556
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT979644.322152199195608No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC117730.5194224188592736No Hit
CCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTGC102020.4501102112632558Illumina PCR Primer Index 5 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTGCT93610.41300545850179743Illumina PCR Primer Index 5 (95% over 24bp)
GCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTGC73110.3225598661581713Illumina PCR Primer Index 5 (95% over 23bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG65750.2900876925167523No Hit
CGCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTG49340.2176870988407081Illumina PCR Primer Index 5 (95% over 22bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCC39260.17321433928832997No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC32690.14422762993722635No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC31820.14038920723776513No Hit
AGCCCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTC30710.13549190931086635No Hit
TCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTGC29250.12905041834395445Illumina PCR Primer Index 5 (95% over 23bp)
GCCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTG28280.1247707976330609Illumina PCR Primer Index 5 (95% over 22bp)
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA27860.12291776598504515No Hit
CGTTCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTC25670.11325552953467728No Hit
AGGCCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTC23850.105225725726609No Hit
GGGCCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTC23820.10509336632317931No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCGTA207.0347375E-445.00000418
CTGCGTA253.8920112E-545.024
AATGCGC351.2126293E-745.09
CGTAAGG302.16644E-644.9999961
CGTTTTT229350.044.2151761
TACGCGG1900.043.815791
TACGGGT1750.041.1428573
CTTACGG2800.040.982141
CACTTAC31050.040.797136
CCGCACT31200.040.67307733
CGCACTT31400.040.62898334
TACGGGA3050.040.5737723
TAGTAGG5050.040.5445561
GCACTTA31300.040.54313335
TAGGGTA6400.040.429694
ACGGGTA1950.040.3846174
ACTTACT30900.040.2669937
TCACGAC3750.040.19999724
ACCCGCA32200.040.17857431
CACGACG3700.040.13513625
Produced by FastQC (version 0.11.5)